Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,592 T→A 39.7% N292Y (AAT→TAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,5920TA39.7% 180.5 / 272.7 219N292Y (AAT→TAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base T (68/64);  new base A (40/47);  total (108/111)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

GGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATT  >  CP009273/3030446‑3030739
                                                                                                                                                  |                                                                                                                                                   
ggCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTg                                                                                                                                                    >  2:2832256/1‑148 (MQ=255)
ggCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTg                                                                                                                                                    >  2:1056083/1‑148 (MQ=255)
  cTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTgag                                                                                                                                                  >  1:295782/1‑148 (MQ=255)
      aaTTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGt                                                                                                                                              <  1:3128624/148‑1 (MQ=255)
      aaTTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGt                                                                                                                                              >  1:573405/1‑148 (MQ=255)
      aaTTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGt                                                                                                                                              >  1:2360428/1‑148 (MQ=255)
       aTTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTg                                                                                                                                             >  2:176141/1‑148 (MQ=255)
         ttGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGc                                                                                                                                           >  2:1464582/1‑148 (MQ=255)
          tGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGcc                                                                                                                                          >  2:1796589/1‑148 (MQ=255)
              ttCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAg                                                                                                                                      >  2:323277/1‑148 (MQ=255)
              ttCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAg                                                                                                                                      >  1:1786710/1‑148 (MQ=255)
                    gTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCaaa                                                                                                                                <  1:2649045/148‑1 (MQ=255)
                     ttCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGGGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCaaaa                                                                                                                               >  2:1267145/1‑148 (MQ=255)
                     ttCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCaaaa                                                                                                                               <  1:2241612/148‑1 (MQ=255)
                          tctcTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTaa                                                                                                                          <  2:3035083/148‑1 (MQ=255)
                          tctcTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTaa                                                                                                                          >  1:712761/1‑148 (MQ=255)
                          tctcTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTaa                                                                                                                          <  1:2778810/148‑1 (MQ=255)
                           ctctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTaaa                                                                                                                         <  2:1887051/148‑1 (MQ=255)
                             ctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATc                                                                                                                       >  1:17258/1‑148 (MQ=255)
                             ctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATc                                                                                                                       <  2:2249783/148‑1 (MQ=255)
                               tttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGc                                                                                                                     <  2:2332549/148‑1 (MQ=255)
                                ttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCt                                                                                                                    <  1:860928/148‑1 (MQ=255)
                                ttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCt                                                                                                                    <  1:2642443/148‑1 (MQ=255)
                                 tCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTg                                                                                                                   <  2:3074784/148‑1 (MQ=255)
                                    cgTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcg                                                                                                                >  2:120289/1‑148 (MQ=255)
                                     gTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcgg                                                                                                               >  2:221078/1‑148 (MQ=255)
                                      tCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcggc                                                                                                              <  2:433239/148‑1 (MQ=255)
                                       cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                             >  2:1231075/1‑148 (MQ=255)
                                       cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                             >  2:1006971/1‑148 (MQ=255)
                                            ttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGttt                                                                                                        <  2:3553403/148‑1 (MQ=255)
                                              aGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGttttt                                                                                                      <  2:1143948/148‑1 (MQ=255)
                                               gcgcATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTtg                                                                                                     <  1:2472943/148‑1 (MQ=255)
                                                    tCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCAt                                                                                                >  2:183041/1‑148 (MQ=255)
                                                     cGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATc                                                                                               <  1:3205252/148‑1 (MQ=255)
                                                      gAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCa                                                                                              <  1:2105389/148‑1 (MQ=255)
                                                           cATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGtt                                                                                         >  2:289539/1‑148 (MQ=255)
                                                                  cAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACt                                                                                  <  1:2355949/148‑1 (MQ=255)
                                                                  cAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACt                                                                                  <  2:2122454/148‑1 (MQ=255)
                                                                   aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                                 <  1:1029534/148‑1 (MQ=255)
                                                                     cACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCa                                                                               <  1:3062408/148‑1 (MQ=255)
                                                                     cACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCa                                                                               <  1:3275191/148‑1 (MQ=255)
                                                                     cACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCa                                                                               >  2:623118/1‑148 (MQ=255)
                                                                            gcTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAgcg                                                                        <  1:2184707/148‑1 (MQ=255)
                                                                            gcTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAgcg                                                                        <  1:2821822/148‑1 (MQ=255)
                                                                              ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCt                                                                      >  2:3542836/1‑148 (MQ=255)
                                                                               tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                     <  1:2661957/148‑1 (MQ=255)
                                                                               tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                     <  1:2982494/148‑1 (MQ=255)
                                                                               tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                     >  2:1596297/1‑148 (MQ=255)
                                                                                cGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCttt                                                                    >  1:2927556/1‑148 (MQ=255)
                                                                                cGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCttt                                                                    >  1:3371463/1‑148 (MQ=255)
                                                                                  ccGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTat                                                                  >  2:2044426/1‑147 (MQ=255)
                                                                                    gATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATc                                                                >  1:2810885/1‑148 (MQ=255)
                                                                                    gATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATc                                                                >  1:1036105/1‑148 (MQ=255)
                                                                                     aTGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcc                                                               >  1:1386188/1‑148 (MQ=255)
                                                                                      tgttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATccc                                                              <  1:1976999/148‑1 (MQ=255)
                                                                                      tgttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATccc                                                              <  2:1562552/148‑1 (MQ=255)
                                                                                       gttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcccc                                                             >  2:2613791/1‑148 (MQ=255)
                                                                                       gttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcccc                                                             >  2:2708832/1‑148 (MQ=255)
                                                                                          gtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGa                                                          <  1:782458/148‑1 (MQ=255)
                                                                                             gCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACgg                                                       <  2:2012935/148‑1 (MQ=255)
                                                                                              cacaACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGc                                                      >  2:539926/1‑148 (MQ=255)
                                                                                                caacaacaGAGCCACACCGACGGACAGATCGTCCAGACGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTGTAATCCCCGGACGGCAc                                                    >  2:3034809/1‑148 (MQ=255)
                                                                                                 aacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACt                                                   >  2:1705884/1‑148 (MQ=255)
                                                                                                   caacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACttt                                                 >  2:2021344/1‑148 (MQ=255)
                                                                                                     acaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTcc                                               >  2:137499/1‑148 (MQ=255)
                                                                                                     acaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTcc                                               >  2:3466072/1‑148 (MQ=255)
                                                                                                     acaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTcc                                               >  2:3466015/1‑148 (MQ=255)
                                                                                                       aGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCgg                                             <  2:1410671/148‑1 (MQ=255)
                                                                                                       aGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCgg                                             <  2:820824/148‑1 (MQ=255)
                                                                                                         cgcCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCt                                           <  2:1361017/148‑1 (MQ=255)
                                                                                                           ccACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCg                                         >  1:306922/1‑148 (MQ=255)
                                                                                                            cacaCCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCgg                                        <  2:3223459/148‑1 (MQ=255)
                                                                                                             acacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGa                                       >  2:2004552/1‑148 (MQ=255)
                                                                                                             acacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGa                                       <  1:3493800/148‑1 (MQ=255)
                                                                                                             acacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGa                                       <  1:1535692/148‑1 (MQ=255)
                                                                                                                ccGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGc                                    <  1:2454735/148‑1 (MQ=255)
                                                                                                                ccGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGc                                    <  1:2695766/148‑1 (MQ=255)
                                                                                                                ccGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGc                                    <  1:3466378/148‑1 (MQ=255)
                                                                                                                 cGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCg                                   <  2:414903/148‑1 (MQ=255)
                                                                                                                 cGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCg                                   >  2:2974096/1‑148 (MQ=255)
                                                                                                                    cGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACt                                <  1:941776/148‑1 (MQ=255)
                                                                                                                     ggACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTc                               <  1:616982/148‑1 (MQ=255)
                                                                                                                       aCATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCAt                             >  1:1067846/1‑148 (MQ=255)
                                                                                                                       aCATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCAt                             >  1:1683560/1‑148 (MQ=255)
                                                                                                                           atCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                         >  1:2113457/1‑148 (MQ=255)
                                                                                                                           atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                         >  2:48009/1‑148 (MQ=255)
                                                                                                                             cGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTg                       <  1:1629988/148‑1 (MQ=255)
                                                                                                                              gTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGc                      <  1:287436/148‑1 (MQ=255)
                                                                                                                               tCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGcc                     <  1:3466026/148‑1 (MQ=255)
                                                                                                                                ccAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCa                    >  1:3537708/1‑148 (MQ=255)
                                                                                                                                        ccGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTCCCGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAACCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGaa            <  2:295782/148‑1 (MQ=255)
                                                                                                                                         cGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAAt           <  2:24111/148‑1 (MQ=255)
                                                                                                                                          ggCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATg          <  1:323277/148‑1 (MQ=255)
                                                                                                                                          ggCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGATTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATg          <  1:120419/148‑1 (MQ=255)
                                                                                                                                          ggCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGATTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATg          <  1:120199/148‑1 (MQ=255)
                                                                                                                                           gcagcaTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGc         >  1:483945/1‑148 (MQ=255)
                                                                                                                                            cagcaTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCg        <  1:1006971/148‑1 (MQ=255)
                                                                                                                                            cagcaTAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCg        <  2:17258/148‑1 (MQ=255)
                                                                                                                                               caTAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGatt     >  1:916163/1‑148 (MQ=255)
                                                                                                                                                 ttGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGattat   >  1:1003348/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATT  >  CP009273/3030446‑3030739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑