Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,041,321 G→T 100% I360I (ATC→ATA)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,041,3210GT95.2% 682.3 / 22.2 230I360I (ATC→ATA) gcvPglycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base G (3/8);  new base T (109/110);  total (112/118)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 230 total aligned reads displayed.

CCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGC  >  CP009273/3041179‑3041464
                                                                                                                                              |                                                                                                                                               
 cGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGt                                                                                                                                           <  2:2676484/148‑1 (MQ=255)
 cGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGt                                                                                                                                           <  2:376465/148‑1 (MQ=255)
 cGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGt                                                                                                                                           <  2:376290/148‑1 (MQ=255)
  gCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTCTACGtt                                                                                                                                          >  2:2393229/1‑148 (MQ=255)
   cTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGttt                                                                                                                                         <  2:3108384/148‑1 (MQ=255)
    ttCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTc                                                                                                                                        >  2:3417082/1‑148 (MQ=255)
        gCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGc                                                                                                                                    <  1:429434/148‑1 (MQ=255)
         cACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGcc                                                                                                                                   >  2:2592819/1‑148 (MQ=255)
           cgcgTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCaa                                                                                                                                 <  1:3120228/148‑1 (MQ=255)
               tCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCgg                                                                                                                             <  1:420365/148‑1 (MQ=255)
               tCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCgg                                                                                                                             <  1:2579844/148‑1 (MQ=255)
                  gTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGcc                                                                                                                          >  2:1794159/1‑148 (MQ=255)
                     cGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTg                                                                                                                       <  1:2346684/148‑1 (MQ=255)
                      gCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGa                                                                                                                      >  1:1606764/1‑148 (MQ=255)
                       cccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGAt                                                                                                                     <  2:3185462/148‑1 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    <  2:1887151/148‑1 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    >  2:1167337/1‑148 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    >  1:3151182/1‑148 (MQ=255)
                        ccGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                    >  1:3148752/1‑148 (MQ=255)
                         cGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATaa                                                                                                                   <  1:3062245/148‑1 (MQ=255)
                          gCTTTGTCGGCCACTTCCACACCCAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATaaa                                                                                                                  <  2:1972808/148‑1 (MQ=255)
                          gCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATaaa                                                                                                                  <  1:871791/148‑1 (MQ=255)
                            tttGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACg                                                                                                                >  2:1987936/1‑148 (MQ=255)
                            tttGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACg                                                                                                                >  1:1172556/1‑148 (MQ=255)
                             ttGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGAAGTTTCATACCTTTTTGTAGAAGGACCGACGCCAGGATATCGGACAGACGGTGAATGAGGTTCGCTATACGTTTCAGTCCAAAAGGGACGAGATAAACgg                                                                                                               >  1:3419874/1‑148 (MQ=255)
                               gTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCa                                                                                                             <  2:1192723/148‑1 (MQ=255)
                                  ggCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATAc                                                                                                          <  1:2490403/148‑1 (MQ=255)
                                  ggCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATAc                                                                                                          <  1:2490369/148‑1 (MQ=255)
                                   gCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACa                                                                                                         >  2:2455529/1‑148 (MQ=255)
                                   gCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACa                                                                                                         >  1:21807/1‑148 (MQ=255)
                                   gCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACa                                                                                                         >  1:1823288/1‑148 (MQ=255)
                                    ccACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAg                                                                                                        >  1:97961/1‑148 (MQ=255)
                                     cACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAgg                                                                                                       <  2:3205016/148‑1 (MQ=255)
                                     cACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAgg                                                                                                       >  2:2867195/1‑148 (MQ=255)
                                      aCTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGGTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGc                                                                                                      <  1:3285530/148‑1 (MQ=255)
                                       cTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCt                                                                                                     <  2:2210138/148‑1 (MQ=255)
                                         tCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTgg                                                                                                   <  1:3384027/148‑1 (MQ=255)
                                         tCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTgg                                                                                                   >  1:3357439/1‑148 (MQ=255)
                                           cacacacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCg                                                                                                 <  2:2241368/148‑1 (MQ=255)
                                           cacacacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCg                                                                                                 <  2:1456521/148‑1 (MQ=255)
                                             cacacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGAt                                                                                               >  2:2335970/1‑148 (MQ=255)
                                               cacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGt                                                                                             <  1:752405/148‑1 (MQ=255)
                                               cacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGt                                                                                             <  1:930220/148‑1 (MQ=255)
                                                acaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGtt                                                                                            <  1:1882776/148‑1 (MQ=255)
                                                acaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGtt                                                                                            >  2:110139/1‑148 (MQ=255)
                                                     gtgtCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCca                                                                                       >  1:2507799/1‑148 (MQ=255)
                                                         cGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGCTTGCcagcag                                                                                   >  2:2976728/1‑148 (MQ=255)
                                                          gAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGt                                                                                  <  1:2652351/148‑1 (MQ=255)
                                                          gAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGt                                                                                  <  2:2855138/148‑1 (MQ=255)
                                                            aaTAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTAc                                                                                >  1:1854723/1‑148 (MQ=255)
                                                              tAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCt                                                                              <  2:2392939/148‑1 (MQ=255)
                                                               aGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTg                                                                             <  1:1269937/148‑1 (MQ=255)
                                                                  gcgcATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGa                                                                          >  2:2131618/1‑148 (MQ=255)
                                                                  gcgcATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGa                                                                          >  2:466700/1‑148 (MQ=255)
                                                                      aTGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTa                                                                      <  2:2861349/148‑1 (MQ=255)
                                                                          cgcAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACaaa                                                                  <  2:1434124/148‑1 (MQ=255)
                                                                            cAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATg                                                                >  1:3077400/1‑148 (MQ=255)
                                                                            cAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATg                                                                <  2:3248017/148‑1 (MQ=255)
                                                                              gTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGtt                                                              >  2:1914007/1‑148 (MQ=255)
                                                                               tttCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTg                                                             >  1:1099581/1‑148 (MQ=255)
                                                                               tttCAGACCTTTATGTAGCAGGCCACCCGCCAGGATATCGGTCAGACGGTGAATGCGGATAGCTCTAAGTTTCAGGCCAACCGGGCCGAGATAAGCGGAAAACAGGCTGGCGATGTATGACATAAGTAACTGGGAAGTACAAATGtag                                                             >  2:294023/1‑146 (MQ=255)
                                                                                   aGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGt                                                         <  1:1813732/148‑1 (MQ=255)
                                                                                    gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGtt                                                        >  1:3205684/1‑148 (MQ=255)
                                                                                    gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGtt                                                        >  1:2116067/1‑148 (MQ=255)
                                                                                    gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGtt                                                        <  2:206099/148‑1 (MQ=255)
                                                                                      ccTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCg                                                      <  1:716889/148‑1 (MQ=255)
                                                                                       cTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGc                                                     <  2:481069/148‑1 (MQ=255)
                                                                                       cTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGc                                                     <  1:1088124/148‑1 (MQ=255)
                                                                                       cTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGc                                                     >  1:1419965/1‑148 (MQ=255)
                                                                                        tttttGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                    >  2:1481381/1‑148 (MQ=255)
                                                                                          tttGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCttt                                                  <  2:1348283/148‑1 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 <  2:1926409/148‑1 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 >  1:1595109/1‑148 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 >  2:3542540/1‑148 (MQ=255)
                                                                                           ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                 <  2:1044948/148‑1 (MQ=255)
                                                                                             gttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtctc                                               <  2:2582489/148‑1 (MQ=255)
                                                                                              ttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCa                                              >  2:893233/1‑148 (MQ=255)
                                                                                                    gCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcgg                                        <  2:2101623/148‑1 (MQ=255)
                                                                                                     cccGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGa                                       <  2:826442/148‑1 (MQ=255)
                                                                                                               ggATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                             >  2:2656016/1‑148 (MQ=255)
                                                                                                               ggATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                             >  2:1783124/1‑148 (MQ=255)
                                                                                                               ggATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                             >  2:3401242/1‑148 (MQ=255)
                                                                                                                gATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcg                            >  1:652617/1‑148 (MQ=255)
                                                                                                                    tCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAg                        >  2:1251262/1‑148 (MQ=255)
                                                                                                                    tCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAg                        <  1:1783124/148‑1 (MQ=255)
                                                                                                                      ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                      <  1:1679740/148‑1 (MQ=255)
                                                                                                                      ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                      <  1:1485343/148‑1 (MQ=255)
                                                                                                                      ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                      <  1:805768/148‑1 (MQ=255)
                                                                                                                        tCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                    <  2:3345668/148‑1 (MQ=255)
                                                                                                                        tCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                    <  2:1753187/148‑1 (MQ=255)
                                                                                                                         cAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGc                   <  1:527308/148‑1 (MQ=255)
                                                                                                                         cAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGc                   <  1:609452/148‑1 (MQ=255)
                                                                                                                           gACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                 >  2:1837150/1‑148 (MQ=255)
                                                                                                                           gACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                 <  1:1028918/148‑1 (MQ=255)
                                                                                                                           gACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGCTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                 <  1:2976728/148‑1 (MQ=255)
                                                                                                                               gTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGcc             >  1:220874/1‑148 (MQ=255)
                                                                                                                                  aaTGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg          <  1:2867195/148‑1 (MQ=255)
                                                                                                                                   aTGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATgc         >  2:765012/1‑148 (MQ=255)
                                                                                                                                         ttAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg   >  2:691338/1‑148 (MQ=255)
                                                                                                                                          tAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcgc  >  1:2041651/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGC  >  CP009273/3041179‑3041464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑