Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 255,487 A→T 100% S109T (TCT→ACT)  phoE ← outer membrane phosphoporin protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273255,4870AT98.9% 580.4 / ‑2.4 180S109T (TCT→ACT) phoEouter membrane phosphoporin protein E
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (93/85);  total (95/85)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 180 total aligned reads displayed.

TGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAA  >  CP009273/255343‑255631
                                                                                                                                                |                                                                                                                                                
 gtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAccc                                                                                                                                              >  2:1029181/1‑148 (MQ=255)
  ttCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCa                                                                                                                                             >  2:2887898/1‑148 (MQ=255)
  ttCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCa                                                                                                                                             >  2:2681072/1‑148 (MQ=255)
     cGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAAt                                                                                                                                          <  1:97517/148‑1 (MQ=255)
        tACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCtt                                                                                                                                       <  2:528162/148‑1 (MQ=255)
               ccAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGAACATAATCGAAAGTACCCAAATCTTTATAttt                                                                                                                                >  2:1218591/1‑148 (MQ=255)
                 aGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCa                                                                                                                              >  2:2474183/1‑148 (MQ=255)
                   aCCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAc                                                                                                                            >  2:3154119/1‑148 (MQ=255)
                           cgcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaaa                                                                                                                    <  1:2326451/148‑1 (MQ=255)
                           cgcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaaa                                                                                                                    <  1:3482242/148‑1 (MQ=255)
                           cgcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaaa                                                                                                                    >  2:1619806/1‑148 (MQ=255)
                             cgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAg                                                                                                                  >  2:1659834/1‑148 (MQ=255)
                               tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCg                                                                                                                <  1:1054163/148‑1 (MQ=255)
                                  ggTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgaga                                                                                                             <  2:1950592/148‑1 (MQ=255)
                                  ggTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgaga                                                                                                             <  2:276599/148‑1 (MQ=255)
                                  ggTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgaga                                                                                                             <  2:276625/148‑1 (MQ=255)
                                  ggTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgaga                                                                                                             <  2:3521132/148‑1 (MQ=255)
                                   gTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAc                                                                                                            >  1:705207/1‑148 (MQ=255)
                                    tCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAcg                                                                                                           >  2:891/1‑148 (MQ=255)
                                         aaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttt                                                                                                      >  2:62626/1‑148 (MQ=255)
                                         aaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttt                                                                                                      >  2:62399/1‑148 (MQ=255)
                                          aGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtttt                                                                                                     <  1:3176855/148‑1 (MQ=255)
                                           gTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttttt                                                                                                    <  1:2600017/148‑1 (MQ=255)
                                             tGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTg                                                                                                  <  1:654111/148‑1 (MQ=255)
                                              gTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGc                                                                                                 <  2:601233/148‑1 (MQ=255)
                                               tCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGACAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                >  1:3146174/1‑148 (MQ=255)
                                               tCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                >  2:2778739/1‑148 (MQ=255)
                                                  gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCtgtg                                                                                             <  2:2346436/148‑1 (MQ=255)
                                                         ccGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATc                                                                                      >  2:1765014/1‑148 (MQ=255)
                                                          cGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCa                                                                                     <  1:2024156/148‑1 (MQ=255)
                                                            aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCAct                                                                                   >  2:2296149/1‑148 (MQ=255)
                                                            aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCAct                                                                                   <  1:2913540/148‑1 (MQ=255)
                                                            aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCAct                                                                                   <  2:518170/148‑1 (MQ=255)
                                                              gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActct                                                                                 >  1:263625/1‑148 (MQ=255)
                                                              gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActct                                                                                 <  2:2000202/148‑1 (MQ=255)
                                                              gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActct                                                                                 >  2:3477236/1‑148 (MQ=255)
                                                               aaTCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActctc                                                                                <  2:718819/148‑1 (MQ=255)
                                                                 tCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTg                                                                              <  2:2958690/148‑1 (MQ=255)
                                                                    ccaccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCtt                                                                           <  1:799077/148‑1 (MQ=255)
                                                                    ccaccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCtt                                                                           <  1:799325/148‑1 (MQ=255)
                                                                     caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCttt                                                                          >  1:2590446/1‑148 (MQ=255)
                                                                     caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCttt                                                                          <  1:2490034/148‑1 (MQ=255)
                                                                       ccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttat                                                                        <  1:3411798/148‑1 (MQ=255)
                                                                         aaaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttatta                                                                      >  2:484299/1‑148 (MQ=255)
                                                                          aaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTAc                                                                     <  2:1744353/148‑1 (MQ=255)
                                                                             tCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCgg                                                                  >  2:1395653/1‑148 (MQ=255)
                                                                                  gAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAAc                                                             >  2:3242799/1‑148 (MQ=255)
                                                                                    aCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActc                                                           >  1:738975/1‑148 (MQ=255)
                                                                                       tatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGc                                                        <  2:2249042/148‑1 (MQ=255)
                                                                                        atCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCt                                                       >  1:2043220/1‑148 (MQ=255)
                                                                                        atCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCt                                                       >  2:2599640/1‑148 (MQ=255)
                                                                                        atCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCt                                                       <  1:555493/148‑1 (MQ=255)
                                                                                          cGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTc                                                     >  1:2897541/1‑148 (MQ=255)
                                                                                              ccAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCaa                                                 >  2:2574046/1‑148 (MQ=255)
                                                                                              ccAGGCGTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCaa                                                 <  2:2293558/148‑1 (MQ=255)
                                                                                                 ggCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGa                                              <  1:2662536/148‑1 (MQ=255)
                                                                                                 ggCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGa                                              <  1:2662896/148‑1 (MQ=255)
                                                                                                  gCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGAc                                             >  2:470145/1‑148 (MQ=255)
                                                                                                     tCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCAt                                          <  2:2265246/148‑1 (MQ=255)
                                                                                                           tCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcag                                    >  2:1706228/1‑148 (MQ=255)
                                                                                                           tCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcag                                    >  1:2373681/1‑148 (MQ=255)
                                                                                                            cATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagt                                   >  2:2177464/1‑148 (MQ=255)
                                                                                                            cATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagt                                   >  2:217973/1‑148 (MQ=255)
                                                                                                            cATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagt                                   <  2:866007/148‑1 (MQ=255)
                                                                                                               aCAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcag                                >  1:586138/1‑148 (MQ=255)
                                                                                                               aCAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcag                                <  2:3484946/148‑1 (MQ=255)
                                                                                                               aCAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcag                                <  1:1003102/148‑1 (MQ=255)
                                                                                                                cAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGTCGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcagt                               >  2:2358324/1‑148 (MQ=255)
                                                                                                                cAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcagt                               >  1:2967963/1‑148 (MQ=255)
                                                                                                                cAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGATTCCCAACGACCATAACcagtcagt                               >  2:2145259/1‑148 (MQ=255)
                                                                                                                  aCGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTg                             <  2:1493346/148‑1 (MQ=255)
                                                                                                                  aCGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTg                             >  1:2344159/1‑148 (MQ=255)
                                                                                                                  aCGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTg                             <  2:1003849/148‑1 (MQ=255)
                                                                                                                   cGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGa                            >  1:1589584/1‑148 (MQ=255)
                                                                                                                   cGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGa                            >  1:1590748/1‑148 (MQ=255)
                                                                                                                      ccccAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCg                         >  2:2392214/1‑148 (MQ=255)
                                                                                                                      ccccAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCg                         <  1:3154119/148‑1 (MQ=255)
                                                                                                                         cAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACACTCTGCTTTATTACCGGCAAACTCTTCTTCCCAACCACCATAAACCGACCGTTGATCGTTa                      >  1:24650/1‑148 (MQ=255)
                                                                                                                          aGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTaa                     >  2:2830932/1‑148 (MQ=255)
                                                                                                                             ttACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                  <  1:173445/148‑1 (MQ=255)
                                                                                                                             ttACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                  <  1:2395886/148‑1 (MQ=255)
                                                                                                                             ttACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                  >  2:2443314/1‑148 (MQ=255)
                                                                                                                             ttACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                  >  2:1138918/1‑148 (MQ=255)
                                                                                                                               aCGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtgt                <  2:1480923/148‑1 (MQ=255)
                                                                                                                               aCGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtgt                <  2:3248653/148‑1 (MQ=255)
                                                                                                                                  aCCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGtt             >  1:517685/1‑148 (MQ=255)
                                                                                                                                   ccATAATCGAAATTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt            <  2:489698/148‑1 (MQ=255)
                                                                                                                                   ccATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt            >  2:1255587/1‑148 (MQ=255)
                                                                                                                                   ccATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt            <  1:1892944/148‑1 (MQ=255)
                                                                                                                                    cATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTc           >  1:3254309/1‑148 (MQ=255)
                                                                                                                                    cATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTc           >  1:2489673/1‑148 (MQ=255)
                                                                                                                                     aTAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCg          >  1:2175811/1‑148 (MQ=255)
                                                                                                                                      tAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGc         >  1:2834075/1‑148 (MQ=255)
                                                                                                                                       aaTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGcc        >  2:3077064/1‑148 (MQ=255)
                                                                                                                                        aTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCt       >  2:1085788/1‑148 (MQ=255)
                                                                                                                                        aTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCt       <  1:2474183/148‑1 (MQ=255)
                                                                                                                                         tCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCtt      <  1:470145/148‑1 (MQ=255)
                                                                                                                                         tCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCtt      <  1:2944373/148‑1 (MQ=255)
                                                                                                                                             aaGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGaa  >  2:2801937/1‑148 (MQ=255)
                                                                                                                                             aaGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGaa  >  2:1313579/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAA  >  CP009273/255343‑255631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑