Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,064,914 G→T 100% I356I (ATC→ATA)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,064,9140GT100.0% 581.6 / NA 185I356I (ATC→ATA) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (86/99);  total (86/99)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACC  >  CP009273/3064769‑3065060
                                                                                                                                                 |                                                                                                                                                  
     aaaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGt                                                                                                                                             <  2:665568/148‑1 (MQ=255)
      aaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTc                                                                                                                                            >  1:944374/1‑148 (MQ=255)
      aaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTc                                                                                                                                            >  2:2365991/1‑148 (MQ=255)
       aGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCa                                                                                                                                           <  1:3485399/148‑1 (MQ=255)
       aGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCa                                                                                                                                           >  2:2271714/1‑148 (MQ=255)
        gcgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAg                                                                                                                                          >  2:3534006/1‑148 (MQ=255)
        gcgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAg                                                                                                                                          >  2:3533808/1‑148 (MQ=255)
         cgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGc                                                                                                                                         >  2:2021879/1‑148 (MQ=255)
         cgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGc                                                                                                                                         >  2:2760696/1‑148 (MQ=255)
             cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGCTGTAGGATATTTTGTCAGCAATg                                                                                                                                     >  2:1905814/1‑148 (MQ=255)
             cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATg                                                                                                                                     >  2:3246796/1‑148 (MQ=255)
               gAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGcc                                                                                                                                   >  1:983529/1‑148 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                               <  1:2869918/148‑1 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                               <  2:1075937/148‑1 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                               <  2:1075760/148‑1 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                               <  2:1509022/148‑1 (MQ=255)
                      gggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAgg                                                                                                                            <  2:1404702/148‑1 (MQ=255)
                      gggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAgg                                                                                                                            >  1:2171700/1‑148 (MQ=255)
                       ggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGt                                                                                                                           <  2:1607632/148‑1 (MQ=255)
                         cAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCg                                                                                                                         <  2:2586712/148‑1 (MQ=255)
                         cAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCg                                                                                                                         >  1:1487636/1‑148 (MQ=255)
                          aGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGa                                                                                                                        <  2:3302505/148‑1 (MQ=255)
                          aGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGa                                                                                                                        <  1:370906/148‑1 (MQ=255)
                          aGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGa                                                                                                                        <  1:754719/148‑1 (MQ=255)
                             tttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                     >  2:586328/1‑148 (MQ=255)
                             tttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                     >  2:586482/1‑148 (MQ=255)
                                     ccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCaga                                                                                                             <  2:1131180/148‑1 (MQ=255)
                                        tgtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgtg                                                                                                          >  1:2105508/1‑148 (MQ=255)
                                         gtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgtgt                                                                                                         >  1:3358934/1‑148 (MQ=255)
                                          tgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTc                                                                                                        >  2:1715553/1‑148 (MQ=255)
                                           gATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcg                                                                                                       <  1:1696586/148‑1 (MQ=255)
                                             ttttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgcc                                                                                                     <  1:1274151/148‑1 (MQ=255)
                                              tttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccg                                                                                                    <  2:3051482/148‑1 (MQ=255)
                                              tttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccg                                                                                                    <  2:3154285/148‑1 (MQ=255)
                                                  tACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgccgc                                                                                                >  1:2872763/1‑148 (MQ=255)
                                                    cttcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCa                                                                                              >  1:1048278/1‑148 (MQ=255)
                                                     ttcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAg                                                                                             >  2:942152/1‑148 (MQ=255)
                                                              cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                    >  1:725414/1‑148 (MQ=255)
                                                              cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                    <  1:1053006/148‑1 (MQ=255)
                                                              cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                    <  1:2062287/148‑1 (MQ=255)
                                                                 tctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACg                                                                                 >  1:224106/1‑148 (MQ=255)
                                                                 tctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACg                                                                                 <  1:2713934/148‑1 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                                >  1:1636490/1‑148 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                                >  1:649691/1‑148 (MQ=255)
                                                                   ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCt                                                                               <  2:1865494/148‑1 (MQ=255)
                                                                       aGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGc                                                                           <  1:3265384/148‑1 (MQ=255)
                                                                        gCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCt                                                                          >  2:2823036/1‑148 (MQ=255)
                                                                        gCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCt                                                                          <  1:2634204/148‑1 (MQ=255)
                                                                          aTCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGt                                                                        <  2:2466197/148‑1 (MQ=255)
                                                                           tCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTc                                                                       >  2:1745245/1‑148 (MQ=255)
                                                                            cGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCt                                                                      >  1:2893548/1‑148 (MQ=255)
                                                                                aCTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGa                                                                  >  1:2302552/1‑148 (MQ=255)
                                                                                 cTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                                 <  2:3037262/148‑1 (MQ=255)
                                                                                 cTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                                 <  2:146122/148‑1 (MQ=255)
                                                                                   gcaggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAg                                                               <  2:271187/148‑1 (MQ=255)
                                                                                     aggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCg                                                             <  2:1604917/148‑1 (MQ=255)
                                                                                     aggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCg                                                             <  2:3366765/148‑1 (MQ=255)
                                                                                      ggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGt                                                            >  2:750745/1‑148 (MQ=255)
                                                                                       gcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGtt                                                           <  2:1234315/148‑1 (MQ=255)
                                                                                           tACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGcc                                                       <  2:983546/148‑1 (MQ=255)
                                                                                           tACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGcc                                                       >  1:3206211/1‑148 (MQ=255)
                                                                                            aCTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCa                                                      >  2:3258073/1‑148 (MQ=255)
                                                                                              tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACg                                                    >  2:1024657/1‑148 (MQ=255)
                                                                                              tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACg                                                    <  2:297255/148‑1 (MQ=255)
                                                                                              tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACg                                                    <  2:2460336/148‑1 (MQ=255)
                                                                                               ttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGa                                                   >  2:1562550/1‑148 (MQ=255)
                                                                                               ttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGa                                                   >  2:1562567/1‑148 (MQ=255)
                                                                                                tACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAt                                                  <  1:2939146/148‑1 (MQ=255)
                                                                                                tACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAt                                                  >  1:1578597/1‑148 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                                >  1:3159645/1‑148 (MQ=255)
                                                                                                   cTTCCACGACTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTc                                               <  2:843213/148‑1 (MQ=255)
                                                                                                   cTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTc                                               >  2:361710/1‑148 (MQ=255)
                                                                                                    ttCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCa                                              <  1:3534006/148‑1 (MQ=255)
                                                                                                    ttCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCa                                              <  1:3533808/148‑1 (MQ=255)
                                                                                                      ccACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGt                                            <  1:2077395/148‑1 (MQ=255)
                                                                                                        aCGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTAc                                          <  1:489550/148‑1 (MQ=255)
                                                                                                        aCGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTAc                                          <  1:1715553/148‑1 (MQ=255)
                                                                                                         cGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTAcc                                         >  1:2035290/1‑148 (MQ=255)
                                                                                                            aTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCttt                                      >  1:1183926/1‑148 (MQ=255)
                                                                                                                 aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGa                                 <  1:353599/148‑1 (MQ=255)
                                                                                                                 aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGa                                 >  1:2474472/1‑148 (MQ=255)
                                                                                                                 aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGa                                 >  1:691373/1‑148 (MQ=255)
                                                                                                                  ggAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGaa                                >  1:2621068/1‑148 (MQ=255)
                                                                                                                  ggAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGaa                                >  1:281715/1‑148 (MQ=255)
                                                                                                                   gAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                               <  2:910382/148‑1 (MQ=255)
                                                                                                                   gAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                               >  1:193203/1‑148 (MQ=255)
                                                                                                                     aTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGtt                             <  1:2638955/148‑1 (MQ=255)
                                                                                                                     aTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGtt                             <  1:2639340/148‑1 (MQ=255)
                                                                                                                      tGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTc                            >  2:2928232/1‑148 (MQ=255)
                                                                                                                          ccgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGa                        >  1:1360993/1‑148 (MQ=255)
                                                                                                                            gccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAAt                      <  1:1083193/148‑1 (MQ=255)
                                                                                                                                ccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGa                  >  1:1240580/1‑148 (MQ=255)
                                                                                                                                  aGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAac                <  1:1115737/148‑1 (MQ=255)
                                                                                                                                   gTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAaca               <  1:380472/148‑1 (MQ=255)
                                                                                                                                     ggAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACg             <  1:3030166/148‑1 (MQ=255)
                                                                                                                                        gaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCa          <  2:2721670/148‑1 (MQ=255)
                                                                                                                                         aTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCaa         <  1:2744441/148‑1 (MQ=255)
                                                                                                                                           gTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAAcc       <  1:1381854/148‑1 (MQ=255)
                                                                                                                                             aGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCgg     <  2:488947/148‑1 (MQ=255)
                                                                                                                                             aGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCgg     >  1:888718/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACC  >  CP009273/3064769‑3065060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑