Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,088,065 C→A 93.8% V125V (GTG→GTT)  yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,088,0650CA93.8% 554.8 / 27.6 194V125V (GTG→GTT) yggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base C (9/3);  new base A (103/79);  total (112/82)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 194 total aligned reads displayed.

CCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAAT  >  CP009273/3087920‑3088209
                                                                                                                                                 |                                                                                                                                                
    ctgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACTGGATCTTCCAGCGTCAGAATATGCGCATCGTCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGa                                                                                                                                            <  1:2376099/148‑1 (MQ=255)
     tgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGaa                                                                                                                                           <  2:294138/148‑1 (MQ=255)
      gctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAAt                                                                                                                                          <  1:3126501/148‑1 (MQ=255)
       ctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATc                                                                                                                                         <  1:3158556/148‑1 (MQ=255)
         ggATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAg                                                                                                                                       >  1:959310/1‑148 (MQ=255)
          gATCAAACATCTCTGGCTGGCATAGATATATTCAACAGGATCTTCCAGCGTCAGTATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCTCCAGCGTGGTAGATTTGCCACTCCGCGTCGCCCCCGTAACCAGAATCAgg                                                                                                                                      <  1:3364063/148‑1 (MQ=255)
          gATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAgg                                                                                                                                      <  1:2803659/148‑1 (MQ=255)
          gATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAgg                                                                                                                                      >  1:3494136/1‑148 (MQ=255)
           aTCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGc                                                                                                                                     <  2:2065536/148‑1 (MQ=255)
            tCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGcc                                                                                                                                    >  2:391504/1‑148 (MQ=255)
             cAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCa                                                                                                                                   <  1:719631/148‑1 (MQ=255)
               aaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAtt                                                                                                                                 >  1:2192011/1‑148 (MQ=255)
                aCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtc                                                                                                                                >  1:2546722/1‑148 (MQ=255)
                aCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtc                                                                                                                                <  2:2896135/148‑1 (MQ=255)
                aCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtc                                                                                                                                >  2:2121487/1‑148 (MQ=255)
                 cATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtct                                                                                                                               >  2:2108940/1‑148 (MQ=255)
                    cGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGc                                                                                                                            >  1:2444405/1‑148 (MQ=255)
                        ggctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCtt                                                                                                                        <  1:1410504/148‑1 (MQ=255)
                         gctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTg                                                                                                                       >  1:2080213/1‑148 (MQ=255)
                            ggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGACTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGt                                                                                                                    >  1:1581402/1‑148 (MQ=255)
                                  gagaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTcc                                                                                                              >  1:862964/1‑148 (MQ=255)
                                  gagaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTcc                                                                                                              >  1:305180/1‑148 (MQ=255)
                                   agaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCg                                                                                                             >  2:1773946/1‑148 (MQ=255)
                                    gaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCgg                                                                                                            >  1:188189/1‑148 (MQ=255)
                                       atTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCaa                                                                                                         >  2:2898195/1‑148 (MQ=255)
                                       atTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCaa                                                                                                         >  1:2711392/1‑148 (MQ=255)
                                       atTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCaa                                                                                                         >  1:2485989/1‑148 (MQ=255)
                                       atTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCaa                                                                                                         <  2:875091/148‑1 (MQ=255)
                                        ttCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAAt                                                                                                        >  2:3418914/1‑148 (MQ=255)
                                        ttCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAAt                                                                                                        >  2:3142321/1‑148 (MQ=255)
                                          cAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATAc                                                                                                      >  1:481530/1‑148 (MQ=255)
                                          cAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATAc                                                                                                      >  1:2272128/1‑148 (MQ=255)
                                            aCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                                                                                    >  2:1652454/1‑148 (MQ=255)
                                            aCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                                                                                    >  1:1574383/1‑148 (MQ=255)
                                            aCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                                                                                    <  2:3494136/148‑1 (MQ=255)
                                             cAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGt                                                                                                   >  1:157297/1‑148 (MQ=255)
                                               ggATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAAGCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                                                 >  1:1265593/1‑148 (MQ=255)
                                                gATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTgg                                                                                                >  2:2458608/1‑148 (MQ=255)
                                                 aTCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGt                                                                                               >  2:154420/1‑148 (MQ=255)
                                                      ccAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGcc                                                                                          <  2:2667931/148‑1 (MQ=255)
                                                      ccAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGcc                                                                                          >  1:1873470/1‑148 (MQ=255)
                                                        aGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCaa                                                                                        >  1:2607459/1‑148 (MQ=255)
                                                         gCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAg                                                                                       <  2:2047202/148‑1 (MQ=255)
                                                            tCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTg                                                                                    >  1:2545328/1‑148 (MQ=255)
                                                             cAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGt                                                                                   <  1:3153724/148‑1 (MQ=255)
                                                                 atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGa                                                                               >  1:1192520/1‑148 (MQ=255)
                                                                 atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGa                                                                               >  2:3329989/1‑148 (MQ=255)
                                                                 atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGa                                                                               >  2:1711085/1‑148 (MQ=255)
                                                                  tatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAg                                                                              >  1:3113147/1‑148 (MQ=255)
                                                                  tatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAg                                                                              <  1:2391921/148‑1 (MQ=255)
                                                                     gcgcATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTg                                                                           >  1:2925737/1‑148 (MQ=255)
                                                                     gcgcATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTg                                                                           >  1:1843095/1‑148 (MQ=255)
                                                                          tCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGc                                                                      >  2:1921631/1‑148 (MQ=255)
                                                                           cGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCa                                                                     <  2:270614/148‑1 (MQ=255)
                                                                             gCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGt                                                                   <  2:3295951/148‑1 (MQ=255)
                                                                              cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTg                                                                  <  1:322328/148‑1 (MQ=255)
                                                                              cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTg                                                                  <  1:21601/148‑1 (MQ=255)
                                                                                tGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCg                                                                >  1:1415689/1‑148 (MQ=255)
                                                                                 gTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                               <  1:1774529/148‑1 (MQ=255)
                                                                                  ttgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGaa                                                              >  2:2256314/1‑148 (MQ=255)
                                                                                   tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCCTTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAg                                                             <  2:1840776/148‑1 (MQ=255)
                                                                                   tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAg                                                             >  2:115534/1‑148 (MQ=255)
                                                                                   tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAg                                                             >  2:2982439/1‑148 (MQ=255)
                                                                                     attgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                           >  2:17506/1‑148 (MQ=255)
                                                                                      ttgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTa                                                          <  1:593170/148‑1 (MQ=255)
                                                                                      ttgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTa                                                          >  1:374704/1‑148 (MQ=255)
                                                                                       tgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTaa                                                         <  1:2174674/148‑1 (MQ=255)
                                                                                           aTAGCCAACCATCGCCGCCAGCGTGTTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGc                                                     <  1:2002930/148‑1 (MQ=255)
                                                                                           aTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGc                                                     <  1:2004947/148‑1 (MQ=255)
                                                                                               ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTa                                                 <  2:937840/148‑1 (MQ=255)
                                                                                               ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTa                                                 <  1:2668529/148‑1 (MQ=255)
                                                                                                  aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACg                                              >  1:1128048/1‑148 (MQ=255)
                                                                                                  aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACg                                              >  1:1416440/1‑148 (MQ=255)
                                                                                                   ccATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGc                                             >  1:3328031/1‑148 (MQ=255)
                                                                                                    cATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGTTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGcc                                            <  1:673405/148‑1 (MQ=255)
                                                                                                    cATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGTTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGcc                                            <  1:2457220/148‑1 (MQ=255)
                                                                                                      tCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAg                                          >  1:2784698/1‑148 (MQ=255)
                                                                                                       cgccgcCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGc                                         >  1:2509010/1‑148 (MQ=255)
                                                                                                          cgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGaa                                      <  2:471373/148‑1 (MQ=255)
                                                                                                           gccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGaaa                                     <  2:2225704/148‑1 (MQ=255)
                                                                                                           gccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGaaa                                     <  2:466059/148‑1 (MQ=255)
                                                                                                           gccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGaaa                                     <  2:1853572/148‑1 (MQ=255)
                                                                                                              aGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgc                                  >  2:518703/1‑148 (MQ=255)
                                                                                                              aGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgc                                  >  1:436770/1‑148 (MQ=255)
                                                                                                               gCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcc                                 <  1:3029498/148‑1 (MQ=255)
                                                                                                                   ggTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatgc                             <  1:259708/148‑1 (MQ=255)
                                                                                                                        aTTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTg                        <  1:891575/148‑1 (MQ=255)
                                                                                                                            gCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGcgcg                    <  1:391504/148‑1 (MQ=255)
                                                                                                                             ccACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGa                   >  1:19400/1‑148 (MQ=255)
                                                                                                                              cACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGaa                  <  2:2546722/148‑1 (MQ=255)
                                                                                                                               aCTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAAt                 <  2:3549056/148‑1 (MQ=255)
                                                                                                                                     cGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTg           >  2:2620194/1‑148 (MQ=255)
                                                                                                                                          cccccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAACTGCCATGCCGTTGCGCGAATGCGCTGCCACg      >  2:586208/1‑148 (MQ=255)
                                                                                                                                          cccccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACg      >  2:1258223/1‑148 (MQ=255)
                                                                                                                                            cccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCa    >  1:1495938/1‑148 (MQ=255)
                                                                                                                                            cccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCa    <  2:2392374/148‑1 (MQ=255)
                                                                                                                                            cccGTAACCAGAATCAGGCCAGTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCa    <  2:2732173/148‑1 (MQ=255)
                                                                                                                                             ccGTAACCAGAATCAGGCCAGTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCaa   <  1:3295089/148‑1 (MQ=255)
                                                                                                                                              cGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAAt  >  2:2153141/1‑148 (MQ=255)
                                                                                                                                              cGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAAt  >  2:1058835/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAAT  >  CP009273/3087920‑3088209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑