Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,122,356 G→T 100% L242F (TTG→TTT)  glcC → glycolate‑inducible glc operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,122,3560GT97.7% 676.3 / 6.3 215L242F (TTG→TTT) glcCglycolate‑inducible glc operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/5);  new base T (86/124);  total (86/129)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 215 total aligned reads displayed.

AAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATG  >  CP009273/3122212‑3122499
                                                                                                                                                |                                                                                                                                               
aaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTAtt                                                                                                                                              >  2:2632155/1‑148 (MQ=255)
 aaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                             >  2:1496536/1‑148 (MQ=255)
   aaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGc                                                                                                                                           <  1:3240788/148‑1 (MQ=255)
   aaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGc                                                                                                                                           >  1:2061636/1‑148 (MQ=255)
    aaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCACACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCt                                                                                                                                          >  2:622088/1‑148 (MQ=255)
     aGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTc                                                                                                                                         >  2:2100129/1‑148 (MQ=255)
      gCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcg                                                                                                                                        >  1:2430807/1‑148 (MQ=255)
           tcgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGt                                                                                                                                   >  2:1111814/1‑148 (MQ=255)
            cgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTg                                                                                                                                  <  2:2478063/148‑1 (MQ=255)
            cgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTg                                                                                                                                  >  2:2717641/1‑148 (MQ=255)
              atcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGcc                                                                                                                                >  1:2693707/1‑148 (MQ=255)
               tcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                               <  2:2686438/148‑1 (MQ=255)
               tcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                               >  1:1687765/1‑148 (MQ=255)
               tcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                               >  1:690616/1‑148 (MQ=255)
               tcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCAGTTTATTCGCTCGGCGGTGCCg                                                                                                                               >  2:3455332/1‑148 (MQ=255)
                cgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGc                                                                                                                              >  1:1840627/1‑148 (MQ=255)
                      cATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGgaga                                                                                                                        >  2:1656359/1‑148 (MQ=255)
                      cATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGAGGTGCCGCTGgaga                                                                                                                        <  1:2362210/148‑1 (MQ=255)
                       aTGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGAt                                                                                                                       <  1:1969102/148‑1 (MQ=255)
                           gcgGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAAc                                                                                                                   <  1:3525925/148‑1 (MQ=255)
                           gcgGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAAc                                                                                                                   <  1:1083658/148‑1 (MQ=255)
                              gATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTg                                                                                                                <  2:1279502/148‑1 (MQ=255)
                              gATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTg                                                                                                                <  1:1603525/148‑1 (MQ=255)
                               aTCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGa                                                                                                               >  2:2899394/1‑148 (MQ=255)
                                 cTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGt                                                                                                             <  1:2194220/148‑1 (MQ=255)
                                      aCGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCt                                                                                                        <  1:1270912/148‑1 (MQ=255)
                                           gtgtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAt                                                                                                   <  2:422846/148‑1 (MQ=255)
                                           gtgtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAt                                                                                                   <  1:1017710/148‑1 (MQ=255)
                                               tGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTAGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTaaa                                                                                               <  1:2340104/148‑1 (MQ=255)
                                                gCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAAt                                                                                              <  1:2523814/148‑1 (MQ=255)
                                                   gCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTg                                                                                           <  2:301926/148‑1 (MQ=255)
                                                    cGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGc                                                                                          <  2:3322491/148‑1 (MQ=255)
                                                      gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCtt                                                                                        >  2:1162330/1‑148 (MQ=255)
                                                      gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCtt                                                                                        <  2:521693/148‑1 (MQ=255)
                                                      gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCtt                                                                                        <  2:2153560/148‑1 (MQ=255)
                                                         gCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTc                                                                                     <  1:809936/148‑1 (MQ=255)
                                                         gCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTc                                                                                     <  1:567736/148‑1 (MQ=255)
                                                          ccGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCa                                                                                    >  2:2171460/1‑148 (MQ=255)
                                                            gCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATa                                                                                  >  2:1577715/1‑148 (MQ=255)
                                                            gCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATa                                                                                  <  2:2636164/148‑1 (MQ=255)
                                                              aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACa                                                                                <  1:389005/148‑1 (MQ=255)
                                                               cGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACaa                                                                               <  1:1332233/148‑1 (MQ=255)
                                                               cGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACaa                                                                               <  1:3254549/148‑1 (MQ=255)
                                                               cGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACaa                                                                               <  1:1668556/148‑1 (MQ=255)
                                                               cGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACaa                                                                               <  2:1361756/148‑1 (MQ=255)
                                                                gTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                              <  1:1551874/148‑1 (MQ=255)
                                                                     ccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                         <  1:162703/148‑1 (MQ=255)
                                                                     ccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                         <  1:591871/148‑1 (MQ=255)
                                                                     ccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                         <  1:3191329/148‑1 (MQ=255)
                                                                      cAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa                                                                        >  2:3303383/1‑148 (MQ=255)
                                                                      cAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa                                                                        >  2:2990116/1‑148 (MQ=255)
                                                                       aGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAAc                                                                       >  1:3031761/1‑148 (MQ=255)
                                                                        gcgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACg                                                                      >  1:2152028/1‑148 (MQ=255)
                                                                            gcagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTg                                                                  <  1:1383555/148‑1 (MQ=255)
                                                                                caCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACg                                                              >  2:1228676/1‑148 (MQ=255)
                                                                                  cgcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGc                                                            >  1:3164205/1‑148 (MQ=255)
                                                                                   gcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                           <  2:1023246/148‑1 (MQ=255)
                                                                                       tCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTCTTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGc                                                       >  2:2824786/1‑148 (MQ=255)
                                                                                       tCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGc                                                       >  2:2958714/1‑148 (MQ=255)
                                                                                        cATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTAGTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCTGTAACGCTTGTACGGCTCCGcc                                                      <  2:3153346/148‑1 (MQ=255)
                                                                                         aTGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGccc                                                     <  2:1443020/148‑1 (MQ=255)
                                                                                         aTGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGccc                                                     <  2:1873683/148‑1 (MQ=255)
                                                                                           gtgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                   >  1:3504897/1‑148 (MQ=255)
                                                                                           gtgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                   <  1:2727192/148‑1 (MQ=255)
                                                                                           gtgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                   >  1:3514061/1‑148 (MQ=255)
                                                                                                gACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCg                                              <  1:2184048/148‑1 (MQ=255)
                                                                                                gACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCg                                              <  1:2793309/148‑1 (MQ=255)
                                                                                                 aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGt                                             >  1:414936/1‑148 (MQ=255)
                                                                                                  ccGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGtt                                            <  2:586711/148‑1 (MQ=255)
                                                                                                   cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc                                           <  2:1397049/148‑1 (MQ=255)
                                                                                                         aaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt                                     >  1:3214814/1‑148 (MQ=255)
                                                                                                           aGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGt                                   <  1:3273991/148‑1 (MQ=255)
                                                                                                              aTCTCCACGATATCGAGCTGGAAGGCCACCATTTTAGTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                <  2:1554160/148‑1 (MQ=255)
                                                                                                               tctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                               >  2:784212/1‑148 (MQ=255)
                                                                                                               tctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                               <  2:180181/148‑1 (MQ=255)
                                                                                                                ctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                              >  1:2760729/1‑148 (MQ=255)
                                                                                                                ctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                              <  2:1105518/148‑1 (MQ=255)
                                                                                                                ctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                              <  2:1995063/148‑1 (MQ=255)
                                                                                                                 tcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                             >  2:2415830/1‑148 (MQ=255)
                                                                                                                   cACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg                           <  2:114007/148‑1 (MQ=255)
                                                                                                                     cGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGcc                         <  1:2857655/148‑1 (MQ=255)
                                                                                                                       atatCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTg                       <  2:2558063/148‑1 (MQ=255)
                                                                                                                         atCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                     <  2:305597/148‑1 (MQ=255)
                                                                                                                            gAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTg                  <  1:1111825/148‑1 (MQ=255)
                                                                                                                               cTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGtt               <  2:1262646/148‑1 (MQ=255)
                                                                                                                               cTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGtt               >  1:312389/1‑148 (MQ=255)
                                                                                                                               cTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGtt               <  1:3440891/148‑1 (MQ=255)
                                                                                                                                 ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc             >  2:150600/1‑148 (MQ=255)
                                                                                                                                 ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc             >  2:3183482/1‑148 (MQ=255)
                                                                                                                                  gAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCg            >  1:1102156/1‑148 (MQ=255)
                                                                                                                                   aaGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa           <  2:1032999/148‑1 (MQ=255)
                                                                                                                                        caccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATc      <  2:1694519/148‑1 (MQ=255)
                                                                                                                                        caccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATc      <  2:414936/148‑1 (MQ=255)
                                                                                                                                         accaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATcc     <  1:298497/148‑1 (MQ=255)
                                                                                                                                         accaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATcc     <  2:3508184/148‑1 (MQ=255)
                                                                                                                                          ccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCa    >  2:2317207/1‑148 (MQ=255)
                                                                                                                                          ccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCa    >  1:573921/1‑148 (MQ=255)
                                                                                                                                          ccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAGTGGTTTCGAGGATCCa    >  2:2011642/1‑148 (MQ=255)
                                                                                                                                           caTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt   >  2:26261/1‑148 (MQ=255)
                                                                                                                                           caTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGGATTGGTTTCGAGGATCCAt   >  2:2105486/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATG  >  CP009273/3122212‑3122499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑