Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,130,160 C→A 5.4% P574P (CCG→CCT)  gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,130,1600CA 5.4% 813.9 / 12.4 212P574P (CCG→CCT) gssfused glutathionylspermidine amidase/glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (76/124);  new base A (9/3);  total (85/127)
Fisher's exact test for biased strand distribution p-value = 1.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCAC  >  CP009273/3130013‑3130306
                                                                                                                                                   |                                                                                                                                                  
caacaTGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTc                                                                                                                                                    <  2:1200401/148‑1 (MQ=255)
     tGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCaa                                                                                                                                               <  2:2164220/148‑1 (MQ=255)
        aTTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAaca                                                                                                                                            <  1:1338039/148‑1 (MQ=255)
          tACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAAcacc                                                                                                                                          >  2:364090/1‑148 (MQ=255)
            cTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACCCGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAAcaccac                                                                                                                                        <  2:573914/148‑1 (MQ=255)
               tttCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGt                                                                                                                                     <  1:2030261/148‑1 (MQ=255)
                ttCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTAGGCAAACACCACAGtt                                                                                                                                    >  1:210579/1‑148 (MQ=255)
                    ccacAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTg                                                                                                                                <  2:1773246/148‑1 (MQ=255)
                    ccacAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTg                                                                                                                                >  1:1531359/1‑148 (MQ=255)
                        aaTTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAg                                                                                                                            <  1:1774508/148‑1 (MQ=255)
                         aTTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGa                                                                                                                           <  2:2107037/148‑1 (MQ=255)
                          ttAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGAGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGAt                                                                                                                          <  1:2736243/148‑1 (MQ=255)
                            aaCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGt                                                                                                                        >  1:2445312/1‑148 (MQ=255)
                            aaCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGt                                                                                                                        <  1:61115/148‑1 (MQ=255)
                             aCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGtt                                                                                                                       <  2:2229630/148‑1 (MQ=255)
                              cGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGttt                                                                                                                      <  2:1166800/148‑1 (MQ=255)
                               ggTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGtttt                                                                                                                     <  2:2015436/148‑1 (MQ=255)
                               ggTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGtttt                                                                                                                     <  2:219041/148‑1 (MQ=255)
                                 ttCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTc                                                                                                                   >  1:1712310/1‑148 (MQ=255)
                                 ttCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTc                                                                                                                   >  2:285845/1‑148 (MQ=255)
                                   cAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTg                                                                                                                 <  2:1139750/148‑1 (MQ=255)
                                   cAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTg                                                                                                                 >  1:2695369/1‑148 (MQ=255)
                                     atatCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCt                                                                                                               <  2:576865/148‑1 (MQ=255)
                                      tatCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTAGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTc                                                                                                              >  1:1062632/1‑148 (MQ=255)
                                       atCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCg                                                                                                             >  1:1267299/1‑148 (MQ=255)
                                        tctctctctTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCgg                                                                                                            <  2:1661274/145‑1 (MQ=255)
                                        tCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCgg                                                                                                            <  2:930679/148‑1 (MQ=255)
                                         cACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGc                                                                                                           >  2:1571607/1‑148 (MQ=255)
                                            tctctTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCaaa                                                                                                        >  1:1415067/1‑148 (MQ=255)
                                            tctctTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCACCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCaaa                                                                                                        >  1:2203443/1‑148 (MQ=255)
                                            tctctTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCACCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCaaa                                                                                                        >  1:1150682/1‑148 (MQ=255)
                                                ttttttGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTa                                                                                                    <  2:1411885/148‑1 (MQ=255)
                                                ttttttGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTa                                                                                                    <  1:802712/148‑1 (MQ=255)
                                                 tttttGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTAc                                                                                                   <  2:469938/148‑1 (MQ=255)
                                                        tgaCCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGt                                                                                            <  2:122651/148‑1 (MQ=255)
                                                         gaCCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGtt                                                                                           <  1:2222846/148‑1 (MQ=255)
                                                         gaCCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGtt                                                                                           <  1:1767973/148‑1 (MQ=255)
                                                            cAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTg                                                                                        <  1:137377/148‑1 (MQ=255)
                                                             aGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGt                                                                                       >  1:1347719/1‑148 (MQ=255)
                                                             aGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGt                                                                                       <  2:223026/148‑1 (MQ=255)
                                                              gTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTc                                                                                      <  1:2909492/148‑1 (MQ=255)
                                                              gTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTc                                                                                      <  2:1495675/148‑1 (MQ=255)
                                                              gTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTc                                                                                      <  2:542032/148‑1 (MQ=255)
                                                               tGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTcc                                                                                     >  1:100999/1‑148 (MQ=255)
                                                                 aTTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAg                                                                                   >  1:2999065/1‑148 (MQ=255)
                                                                 aTTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAg                                                                                   <  2:3318267/148‑1 (MQ=255)
                                                                  ttCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGc                                                                                  <  2:2431409/148‑1 (MQ=255)
                                                                       caCCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACctc                                                                             <  1:1179177/148‑1 (MQ=255)
                                                                       caCCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACctc                                                                             >  2:490718/1‑148 (MQ=255)
                                                                       caCCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACctc                                                                             >  1:3250625/1‑148 (MQ=255)
                                                                       caCCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACctc                                                                             <  1:2860146/148‑1 (MQ=255)
                                                                         ccGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCtt                                                                           >  1:2346200/1‑148 (MQ=255)
                                                                         ccGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCtt                                                                           >  2:542953/1‑148 (MQ=255)
                                                                           gcgcAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCa                                                                         <  2:3402185/148‑1 (MQ=255)
                                                                            cgcAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTAGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCat                                                                        >  1:3483721/1‑148 (MQ=255)
                                                                            cgcAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTAGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCGCTTCat                                                                        >  1:1982501/1‑148 (MQ=255)
                                                                             gcAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCatg                                                                       <  1:1594254/148‑1 (MQ=255)
                                                                              cAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCatga                                                                      >  1:2189606/1‑148 (MQ=255)
                                                                                  cacGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGc                                                                  <  2:584362/148‑1 (MQ=255)
                                                                                    cGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTg                                                                <  2:2361268/148‑1 (MQ=255)
                                                                                      tCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTAGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGAc                                                              >  2:3208627/1‑148 (MQ=255)
                                                                                        ccACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGa                                                            <  1:2818372/148‑1 (MQ=255)
                                                                                         cACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAg                                                           <  2:721828/148‑1 (MQ=255)
                                                                                             gTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGTCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCg                                                       <  2:2293506/148‑1 (MQ=255)
                                                                                             gTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCg                                                       <  2:841364/148‑1 (MQ=255)
                                                                                             gTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCg                                                       <  2:2621903/148‑1 (MQ=255)
                                                                                              tAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGa                                                      >  2:3493203/1‑148 (MQ=255)
                                                                                               aGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGat                                                     >  1:1433944/1‑148 (MQ=255)
                                                                                                gTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGata                                                    <  2:1781527/148‑1 (MQ=255)
                                                                                                 ttGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGatat                                                   >  2:1239516/1‑148 (MQ=255)
                                                                                                 ttGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGatat                                                   >  1:3433737/1‑148 (MQ=255)
                                                                                                      gccAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATtgctg                                              <  2:1322700/148‑1 (MQ=255)
                                                                                                      gccAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGAGAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATtgctg                                              >  2:2031034/1‑148 (MQ=255)
                                                                                                         aaCGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCca                                           <  1:3190915/148‑1 (MQ=255)
                                                                                                          aCGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCcac                                          >  2:2547687/1‑148 (MQ=255)
                                                                                                           cGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCcaca                                         >  1:3298996/1‑148 (MQ=255)
                                                                                                               gAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGa                                     <  2:1303567/148‑1 (MQ=255)
                                                                                                               gAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGa                                     <  1:729893/148‑1 (MQ=255)
                                                                                                                aaGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGAc                                    <  2:286394/148‑1 (MQ=255)
                                                                                                                   gTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCgg                                 >  2:885548/1‑148 (MQ=255)
                                                                                                                      cATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGa                              <  1:451358/148‑1 (MQ=255)
                                                                                                                       aTACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGAt                             >  2:752846/1‑148 (MQ=255)
                                                                                                                       aTACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGAt                             <  2:1808354/148‑1 (MQ=255)
                                                                                                                        tACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATc                            >  2:168418/1‑148 (MQ=255)
                                                                                                                           cTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGt                         <  1:64259/148‑1 (MQ=255)
                                                                                                                            tGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGtt                        >  2:2679077/1‑148 (MQ=255)
                                                                                                                              aaTGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTc                      <  1:1200310/148‑1 (MQ=255)
                                                                                                                                tGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCAc                    <  1:1181602/148‑1 (MQ=255)
                                                                                                                                     ttACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGt               >  2:2061729/1‑148 (MQ=255)
                                                                                                                                     ttACCGTCCACTTTAGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGt               >  1:90019/1‑148 (MQ=255)
                                                                                                                                       aCCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTaa             >  1:165424/1‑148 (MQ=255)
                                                                                                                                        ccGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAAc            <  2:215292/148‑1 (MQ=255)
                                                                                                                                            ccACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGt        <  1:2110032/148‑1 (MQ=255)
                                                                                                                                             cACTTTCGGCAAACACCACATTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTAATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGtt       <  1:3034789/148‑1 (MQ=255)
                                                                                                                                              aCTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGttt      <  2:412316/148‑1 (MQ=255)
                                                                                                                                              aCTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGttt      <  1:1207493/148‑1 (MQ=255)
                                                                                                                                                tttCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTc    >  1:3203066/1‑148 (MQ=255)
                                                                                                                                                tttCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTc    >  1:657811/1‑148 (MQ=255)
                                                                                                                                                tttCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTc    <  1:2057868/148‑1 (MQ=255)
                                                                                                                                                  tCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCAc  <  1:364090/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGAATGTATTTACCGTCCACTTTCGGCAAACACCACAGTTGCTGATAGATGTTTTTCTGCTCGGCAAATTTACCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCAC  >  CP009273/3130013‑3130306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑