Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,186,181 G→C 8.6% W205C (TGG→TGC)  yqiJ → DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,186,1810GC 8.6% 1023.4 / 68.4 278W205C (TGG→TGC) yqiJDUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (123/131);  new base C (9/15);  total (132/146)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

CTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCG  >  CP009273/3186034‑3186328
                                                                                                                                                   |                                                                                                                                                   
cTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTgg                                                                                                                                                     >  1:2664513/1‑148 (MQ=255)
 tGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGc                                                                                                                                                    >  1:1155141/1‑148 (MQ=255)
 tGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGc                                                                                                                                                    <  1:1914702/148‑1 (MQ=255)
   cGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGccc                                                                                                                                                  >  1:745256/1‑148 (MQ=255)
      aGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCaa                                                                                                                                               >  1:2631961/1‑148 (MQ=255)
      aGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCaa                                                                                                                                               >  1:2321152/1‑148 (MQ=255)
       ggAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCaaa                                                                                                                                              <  1:3317514/148‑1 (MQ=255)
        gAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAat                                                                                                                                             >  1:1292233/1‑148 (MQ=255)
         aaaaCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAata                                                                                                                                            <  1:779201/148‑1 (MQ=255)
          aaaCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAatat                                                                                                                                           >  2:1024008/1‑148 (MQ=255)
          aaaCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAatat                                                                                                                                           <  1:2850356/148‑1 (MQ=255)
             cTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGt                                                                                                                                        >  1:595357/1‑148 (MQ=255)
               cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTaa                                                                                                                                      <  1:550576/148‑1 (MQ=255)
               cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTaa                                                                                                                                      <  1:3132307/148‑1 (MQ=255)
               cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTaa                                                                                                                                      >  1:222112/1‑148 (MQ=255)
               cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTaa                                                                                                                                      >  1:2032923/1‑148 (MQ=255)
               cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTaa                                                                                                                                      <  2:363664/148‑1 (MQ=255)
               cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGAGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTaa                                                                                                                                      >  2:542178/1‑148 (MQ=255)
                aCCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAAt                                                                                                                                     <  2:1378365/148‑1 (MQ=255)
                 ccGATCAATTTGGCCAACTACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAAtt                                                                                                                                    <  1:2315392/148‑1 (MQ=255)
                   gATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATtta                                                                                                                                  <  2:3489018/148‑1 (MQ=255)
                   gATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATtta                                                                                                                                  >  2:2114119/1‑148 (MQ=255)
                    aTCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCAAAATATTGTAATttat                                                                                                                                 >  1:1763363/1‑148 (MQ=255)
                         tttGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATc                                                                                                                            >  1:2696607/1‑148 (MQ=255)
                            ggCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCaaa                                                                                                                         <  1:2661615/148‑1 (MQ=255)
                                aaaTACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGa                                                                                                                     <  2:928024/148‑1 (MQ=255)
                                 aaTACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGaa                                                                                                                    <  1:2180837/148‑1 (MQ=255)
                                   tACATTATTTGCTACTGGAGCCTTAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAAt                                                                                                                  >  2:736669/1‑148 (MQ=255)
                                   tACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAAt                                                                                                                  <  1:3416056/148‑1 (MQ=255)
                                   tACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAAt                                                                                                                  <  2:3189682/148‑1 (MQ=255)
                                    aCATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAAtt                                                                                                                 >  2:2803709/1‑148 (MQ=255)
                                       ttattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaa                                                                                                              >  2:3407614/1‑148 (MQ=255)
                                        tattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaat                                                                                                             >  1:1835829/1‑148 (MQ=255)
                                         attTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaata                                                                                                            <  2:1236814/148‑1 (MQ=255)
                                          tttACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaataa                                                                                                           <  1:751762/148‑1 (MQ=255)
                                             actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATgg                                                                                                        >  2:1499399/1‑148 (MQ=255)
                                             actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATgg                                                                                                        >  2:1142426/1‑148 (MQ=255)
                                                actGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatg                                                                                                     <  1:459464/148‑1 (MQ=255)
                                                       ccTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGt                                                                                              <  2:1928130/148‑1 (MQ=255)
                                                       ccTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGt                                                                                              >  1:1546064/1‑148 (MQ=255)
                                                          gaagaaGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTaa                                                                                           >  2:309302/1‑148 (MQ=255)
                                                          gaagaaGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTaa                                                                                           <  1:1603992/148‑1 (MQ=255)
                                                          gaagaaGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTaa                                                                                           <  2:2495937/148‑1 (MQ=255)
                                                           aagaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAt                                                                                          <  2:800213/148‑1 (MQ=255)
                                                            agaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAtt                                                                                         <  1:3407456/148‑1 (MQ=255)
                                                              aagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCt                                                                                       <  2:1894518/148‑1 (MQ=255)
                                                               cggAAAAATTTTCACGACAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCtg                                                                                      <  2:2696607/147‑1 (MQ=255)
                                                               agGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCtg                                                                                      <  1:14932/148‑1 (MQ=255)
                                                                ggAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCtgt                                                                                     <  1:651629/148‑1 (MQ=255)
                                                                   aaaaTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGcc                                                                                  >  1:2974680/1‑148 (MQ=255)
                                                                      aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctc                                                                               >  2:3210914/1‑148 (MQ=255)
                                                                       ttttCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctcc                                                                              <  1:846474/148‑1 (MQ=255)
                                                                            aCTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGAt                                                                         >  2:2934638/1‑148 (MQ=255)
                                                                               aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                      <  2:2977035/148‑1 (MQ=255)
                                                                               aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                      >  1:2276737/1‑148 (MQ=255)
                                                                               aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                      >  1:2521330/1‑148 (MQ=255)
                                                                                  ggAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAc                                                                   >  1:1890878/1‑148 (MQ=255)
                                                                                  ggAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAc                                                                   >  1:241815/1‑148 (MQ=255)
                                                                                  ggAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAc                                                                   <  2:2164703/148‑1 (MQ=255)
                                                                                         aaGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatt                                                            >  2:3325747/1‑148 (MQ=255)
                                                                                         aaGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatt                                                            >  2:1660388/1‑148 (MQ=255)
                                                                                          aGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatta                                                           <  1:1963484/148‑1 (MQ=255)
                                                                                           gtgtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAattat                                                          <  2:2410200/148‑1 (MQ=255)
                                                                                           gtgtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAattat                                                          >  1:1044137/1‑148 (MQ=255)
                                                                                           gtgtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAattat                                                          >  1:508565/1‑148 (MQ=255)
                                                                                               tAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGcc                                                      <  1:3017869/148‑1 (MQ=255)
                                                                                               tAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGcc                                                      <  1:2114119/148‑1 (MQ=255)
                                                                                                 attattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGt                                                    >  1:2794160/1‑148 (MQ=255)
                                                                                                  ttattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTa                                                   >  2:835234/1‑148 (MQ=255)
                                                                                                  ttattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTa                                                   >  2:2330304/1‑148 (MQ=255)
                                                                                                    attTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATg                                                 <  2:1546132/148‑1 (MQ=255)
                                                                                                        gCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCAtt                                             >  1:691247/1‑148 (MQ=255)
                                                                                                        gCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCAtt                                             <  1:1989253/148‑1 (MQ=255)
                                                                                                        gCCGACTGTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCAtt                                             <  2:341131/148‑1 (MQ=255)
                                                                                                         ccGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTc                                            >  1:1142/1‑148 (MQ=255)
                                                                                                              tttCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGttt                                       >  1:3472616/1‑148 (MQ=255)
                                                                                                              tttCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGttt                                       >  1:3504142/1‑148 (MQ=255)
                                                                                                              tttCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGttt                                       <  1:435846/148‑1 (MQ=255)
                                                                                                                tCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttat                                     <  1:3225459/148‑1 (MQ=255)
                                                                                                                 cTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttatt                                    <  2:1292233/148‑1 (MQ=255)
                                                                                                                  tGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttatta                                   >  2:222513/1‑148 (MQ=255)
                                                                                                                     aaCGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTg                                <  1:90561/148‑1 (MQ=255)
                                                                                                                     aaCGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTg                                <  2:532842/148‑1 (MQ=255)
                                                                                                                      aCGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTgg                               <  2:595357/148‑1 (MQ=255)
                                                                                                                      aCGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTgg                               >  1:102216/1‑148 (MQ=255)
                                                                                                                        gcgATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGaa                             <  1:1499399/148‑1 (MQ=255)
                                                                                                                         cgATATTTGGCGTAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAat                            <  2:2707426/148‑1 (MQ=255)
                                                                                                                           atatTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAatta                          <  2:745259/148‑1 (MQ=255)
                                                                                                                            tatTTGTCGGAAATTCTTCCCTGGCCCCAATTTTTGTAATTTATTAATCAAAAGGAATTTTAATAATGGATGATATTGTTTATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCAGTATGCATTCTGTTTATTATTGGAtttat                         <  1:542178/148‑1 (MQ=255)
                                                                                                                               ttGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAGTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTAtttt                      <  1:309302/148‑1 (MQ=255)
                                                                                                                                  gCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGc                   <  1:1241926/148‑1 (MQ=255)
                                                                                                                                   cGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGcc                  <  2:1066589/148‑1 (MQ=255)
                                                                                                                                      aaaaTAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAgg               >  1:1722658/1‑148 (MQ=255)
                                                                                                                                        aataatCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAACGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGct             >  1:386260/1‑148 (MQ=255)
                                                                                                                                         ataatCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGctc            <  2:2032923/148‑1 (MQ=255)
                                                                                                                                          taatCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGctct           <  1:1908064/148‑1 (MQ=255)
                                                                                                                                          taatCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGctct           <  2:1155141/148‑1 (MQ=255)
                                                                                                                                             tCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtc        >  2:410550/1‑148 (MQ=255)
                                                                                                                                             tCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtc        <  2:872379/148‑1 (MQ=255)
                                                                                                                                                   gCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTcgcg  <  2:3472616/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCG  >  CP009273/3186034‑3186328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑