Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,193,107 C→A 5.1% A406A (GCG→GCT)  ygiF ← inorganic triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,193,1070CA 5.1% 826.6 / 13.4 213A406A (GCG→GCT) ygiFinorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base C (110/92);  new base A (5/6);  total (115/98)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 214 total aligned reads displayed.

TCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGG  >  CP009273/3192961‑3193251
                                                                                                                                                  |                                                                                                                                                
   gCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCaa                                                                                                                                              <  1:1265783/148‑1 (MQ=255)
   gCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCaa                                                                                                                                              >  2:3100636/1‑148 (MQ=255)
   gCCAGTACTGCTGTAACGGTGAACAGAGCGGCTTCATAAGCCATTTTATCCTTGCCTGATTAACGATTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGAGTTGCCCGGTCGCaa                                                                                                                                              >  2:378497/1‑148 (MQ=255)
       gTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGc                                                                                                                                          >  1:983509/1‑148 (MQ=255)
       gTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGc                                                                                                                                          >  2:473451/1‑148 (MQ=255)
       gTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGc                                                                                                                                          <  2:1008089/148‑1 (MQ=255)
        tACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCg                                                                                                                                         <  2:3457583/148‑1 (MQ=255)
        tACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCg                                                                                                                                         <  2:1297729/148‑1 (MQ=255)
          ctgctgTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTg                                                                                                                                       <  2:3169600/148‑1 (MQ=255)
          ctgctgTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATAACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTg                                                                                                                                       <  2:3169567/148‑1 (MQ=255)
             ctgTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATg                                                                                                                                    <  2:2295650/148‑1 (MQ=255)
                tAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAg                                                                                                                                 >  2:260424/1‑148 (MQ=255)
                tAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAg                                                                                                                                 <  1:1816407/148‑1 (MQ=255)
                  aCGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGcc                                                                                                                               >  2:2817825/1‑148 (MQ=255)
                     gTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCt                                                                                                                            >  1:2192103/1‑148 (MQ=255)
                      tGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTg                                                                                                                           <  1:339909/148‑1 (MQ=255)
                        aaGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGcc                                                                                                                         <  2:302567/148‑1 (MQ=255)
                        aaGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTAGCAATAGCGTGATGCAGCCCCTGcc                                                                                                                         <  2:1791702/148‑1 (MQ=255)
                        aaGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTAGCAATAGCGTGATGCAGCCCCTGcc                                                                                                                         <  2:245464/148‑1 (MQ=255)
                        aaGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGCTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGcc                                                                                                                         >  2:956869/1‑148 (MQ=255)
                               cGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTtctc                                                                                                                  >  2:1087364/1‑148 (MQ=255)
                                  cTTCATAAGCGATTTTATCCTTGCCTGATAAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATTCAGCCCCTGCCAGTTCTccag                                                                                                               >  2:1666326/1‑148 (MQ=255)
                                   ttCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTccagc                                                                                                              >  2:1228628/1‑148 (MQ=255)
                                    tCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTccagcc                                                                                                             <  2:653585/148‑1 (MQ=255)
                                     cATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTccagcca                                                                                                            <  1:2914563/148‑1 (MQ=255)
                                          gCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCtt                                                                                                       <  2:1633522/148‑1 (MQ=255)
                                                ttctCCGTGCCTGATTAACGTTGTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGcga                                                                                                 <  2:2519204/145‑1 (MQ=255)
                                                ttATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGcga                                                                                                 <  1:352419/148‑1 (MQ=255)
                                                  aTCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGcgacg                                                                                               <  1:1239705/148‑1 (MQ=255)
                                                   tCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGcgacga                                                                                              >  1:1311194/1‑148 (MQ=255)
                                                    ccTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCgacgac                                                                                             >  2:1984026/1‑148 (MQ=255)
                                                     cTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTAGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACa                                                                                            <  1:990504/148‑1 (MQ=255)
                                                      ttGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAg                                                                                           >  1:3031150/1‑148 (MQ=255)
                                                        gCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGa                                                                                         <  1:2478900/148‑1 (MQ=255)
                                                         ccTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGAt                                                                                        >  2:621482/1‑148 (MQ=255)
                                                          cTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATc                                                                                       <  1:3202681/148‑1 (MQ=255)
                                                           tGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCa                                                                                      >  2:1011518/1‑148 (MQ=255)
                                                            gATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCTCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCAt                                                                                     <  1:3172415/148‑1 (MQ=255)
                                                            gATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCAt                                                                                     <  1:3482153/148‑1 (MQ=255)
                                                             aTTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGTAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATa                                                                                    >  2:3126637/1‑148 (MQ=255)
                                                             aTTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATa                                                                                    <  1:573765/148‑1 (MQ=255)
                                                              ttAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAg                                                                                   <  1:2999273/148‑1 (MQ=255)
                                                                  cGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAAc                                                                               <  2:1950643/148‑1 (MQ=255)
                                                                   gTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAAcc                                                                              <  1:1812511/148‑1 (MQ=255)
                                                                     tttCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCg                                                                            >  1:85395/1‑148 (MQ=255)
                                                                     tttCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCg                                                                            >  1:35304/1‑148 (MQ=255)
                                                                          gCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGc                                                                       >  2:1417142/1‑148 (MQ=255)
                                                                             gtgCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAAc                                                                    >  2:3240068/1‑148 (MQ=255)
                                                                               gCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAg                                                                  <  2:838525/148‑1 (MQ=255)
                                                                                cAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGt                                                                 >  1:2753053/1‑148 (MQ=255)
                                                                                 aaCCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTa                                                                <  1:1122377/148‑1 (MQ=255)
                                                                                 aaCCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTa                                                                <  2:3060406/148‑1 (MQ=255)
                                                                                  aCCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTAt                                                               >  2:820264/1‑148 (MQ=255)
                                                                                  aCCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTAt                                                               <  2:613353/148‑1 (MQ=255)
                                                                                    cAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTg                                                             <  1:90737/148‑1 (MQ=255)
                                                                                    cAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTg                                                             <  2:215966/148‑1 (MQ=255)
                                                                                     aGAACGGTTCCTGATTGTTTGCCTCATTACGGAACTGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGGTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATAGa                                                            >  2:3316807/1‑148 (MQ=255)
                                                                                     aGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGa                                                            >  1:2061127/1‑148 (MQ=255)
                                                                                     aGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGa                                                            <  1:2982275/148‑1 (MQ=255)
                                                                                      gAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAg                                                           >  1:1012794/1‑148 (MQ=255)
                                                                                      gAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAg                                                           >  1:1675032/1‑148 (MQ=255)
                                                                                      gAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAg                                                           >  1:3193018/1‑148 (MQ=255)
                                                                                       aaCGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGt                                                          >  2:2481060/1‑148 (MQ=255)
                                                                                         cGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCa                                                        <  2:1916639/148‑1 (MQ=255)
                                                                                          ggTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCaa                                                       <  1:2817825/148‑1 (MQ=255)
                                                                                            ttCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAata                                                     <  1:1995429/148‑1 (MQ=255)
                                                                                            ttCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAata                                                     >  1:2432818/1‑148 (MQ=255)
                                                                                            ttCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAata                                                     >  1:1159259/1‑148 (MQ=255)
                                                                                             tCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAatat                                                    >  1:2287639/1‑148 (MQ=255)
                                                                                             tCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAatat                                                    >  2:3185601/1‑148 (MQ=255)
                                                                                             tCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTAGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAatat                                                    >  1:1679451/1‑148 (MQ=255)
                                                                                                 gATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCAcg                                                <  2:1382652/148‑1 (MQ=255)
                                                                                                       ttGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAg                                          >  1:137640/1‑148 (MQ=255)
                                                                                                          ccTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCATCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCg                                       >  1:1653849/1‑148 (MQ=255)
                                                                                                          ccTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCg                                       <  1:1972836/148‑1 (MQ=255)
                                                                                                            tCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTg                                     >  2:488999/1‑148 (MQ=255)
                                                                                                             cATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTgg                                    >  1:1584505/1‑148 (MQ=255)
                                                                                                             cATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTgg                                    >  1:3261867/1‑148 (MQ=255)
                                                                                                                 aCGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAAc                                >  2:2250808/1‑148 (MQ=255)
                                                                                                                     aaaTGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGt                            >  1:3042270/1‑148 (MQ=255)
                                                                                                                       aTGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCa                          <  2:1648578/148‑1 (MQ=255)
                                                                                                                       aTGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCa                          <  1:1417142/148‑1 (MQ=255)
                                                                                                                       aTGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCa                          <  2:449901/148‑1 (MQ=255)
                                                                                                                        tGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCACCTGGTCAc                         >  2:2638625/1‑148 (MQ=255)
                                                                                                                        tGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCAc                         >  2:1488400/1‑148 (MQ=255)
                                                                                                                         gTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACg                        >  2:213446/1‑148 (MQ=255)
                                                                                                                              aTTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGc                   >  2:739734/1‑148 (MQ=255)
                                                                                                                               tttCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCg                  >  1:420022/1‑148 (MQ=255)
                                                                                                                                ttCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGa                 >  1:820344/1‑148 (MQ=255)
                                                                                                                                 tCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGAt                <  1:3134714/148‑1 (MQ=255)
                                                                                                                                  cGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATc               >  2:2343642/1‑148 (MQ=255)
                                                                                                                                  cGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATc               >  2:38092/1‑148 (MQ=255)
                                                                                                                                    aTGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGc             <  2:328096/148‑1 (MQ=255)
                                                                                                                                    aTGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGc             <  2:2814230/148‑1 (MQ=255)
                                                                                                                                     tGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGcc            >  2:1942953/1‑148 (MQ=255)
                                                                                                                                     tGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGcc            >  1:2535832/1‑148 (MQ=255)
                                                                                                                                         ttGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAAc        >  2:2995219/1‑148 (MQ=255)
                                                                                                                                            cccgcgcGCACTCGCGCGATGCAGCCCCCCCCACGTCTCCAGCCACGCGTGCGCGACGACAGGATCAGAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGc     <  1:3100638/142‑1 (MQ=255)
                                                                                                                                            cccGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGc     <  1:3100577/148‑1 (MQ=255)
                                                                                                                                               ggTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTgg  >  2:2769213/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGG  >  CP009273/3192961‑3193251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑