Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,230,857 C→G 100% A63G (GCC→GGC)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,230,8570CG98.1% 861.5 / 4.9 215A63G (GCC→GGC) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base G (112/99);  total (114/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 217 total aligned reads displayed.

CCAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCG  >  CP009273/3230711‑3231001
                                                                                                                                                  |                                                                                                                                                
ccAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGcc                                                                                                                                                 >  1:368296/1‑148 (MQ=255)
    ttCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTg                                                                                                                                             <  2:1737412/148‑1 (MQ=255)
    ttCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTg                                                                                                                                             <  1:1789835/148‑1 (MQ=255)
     tcgtcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGa                                                                                                                                            >  2:2810414/1‑148 (MQ=255)
       gtcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGAcg                                                                                                                                          <  2:763384/148‑1 (MQ=255)
            ggCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgtg                                                                                                                                     >  2:1192775/1‑148 (MQ=255)
             gccgccCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGgtgt                                                                                                                                    <  2:658948/148‑1 (MQ=255)
               cgccCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGtat                                                                                                                                  <  2:2720542/148‑1 (MQ=255)
                 cccATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTatat                                                                                                                                <  1:2283081/148‑1 (MQ=255)
                     tGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGcc                                                                                                                            >  1:3231272/1‑148 (MQ=255)
                       agagCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAg                                                                                                                          >  1:900438/1‑148 (MQ=255)
                         agCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGcc                                                                                                                        <  1:3344902/148‑1 (MQ=255)
                            ggTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGa                                                                                                                     <  2:1823127/148‑1 (MQ=255)
                                aaTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTc                                                                                                                 <  1:1884219/148‑1 (MQ=255)
                                  tACAAGTTCACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTccc                                                                                                               <  2:2806883/148‑1 (MQ=255)
                                  tACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTccc                                                                                                               >  1:205448/1‑148 (MQ=255)
                                     aaGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGc                                                                                                            <  1:1672018/148‑1 (MQ=255)
                                     aaGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGc                                                                                                            <  1:1076827/148‑1 (MQ=255)
                                       gTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAt                                                                                                          >  2:2162318/1‑148 (MQ=255)
                                       gTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAt                                                                                                          >  2:2162532/1‑148 (MQ=255)
                                        ttAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTTCCAGCCCGAATTCCCTGCAtt                                                                                                         >  2:2103458/1‑148 (MQ=255)
                                        ttAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAtt                                                                                                         <  1:1614655/148‑1 (MQ=255)
                                        ttAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAtt                                                                                                         <  1:2563021/148‑1 (MQ=255)
                                         tAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAttt                                                                                                        >  2:2608876/1‑148 (MQ=255)
                                          aaCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAtttt                                                                                                       <  1:62116/148‑1 (MQ=255)
                                           aCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAttttt                                                                                                      <  2:3472525/148‑1 (MQ=255)
                                           aCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAttttt                                                                                                      <  2:2180781/148‑1 (MQ=255)
                                           aCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAttttt                                                                                                      <  2:3510745/148‑1 (MQ=255)
                                            ccgccgTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTc                                                                                                     >  1:1110281/1‑148 (MQ=255)
                                                  tataTTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGac                                                                                               >  1:3237986/1‑148 (MQ=255)
                                                   atatTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGaca                                                                                              >  2:3483442/1‑148 (MQ=255)
                                                      ttCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACaa                                                                                           <  1:1115418/148‑1 (MQ=255)
                                                      ttCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACaa                                                                                           >  1:1794212/1‑148 (MQ=255)
                                                       tCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAAc                                                                                          >  2:512258/1‑148 (MQ=255)
                                                       tCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCACAGACACAAc                                                                                          >  2:3292581/1‑148 (MQ=255)
                                                          cGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACttt                                                                                       >  2:1192389/1‑148 (MQ=255)
                                                           gCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATATGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACTAGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACTCAACtttt                                                                                      <  2:3173807/148‑1 (MQ=255)
                                                            cAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTc                                                                                     >  2:3529757/1‑148 (MQ=255)
                                                            cAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTc                                                                                     >  1:1540851/1‑148 (MQ=255)
                                                            cAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTc                                                                                     >  2:528954/1‑148 (MQ=255)
                                                             aGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTcc                                                                                    >  1:286442/1‑148 (MQ=255)
                                                             aGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTcc                                                                                    <  2:872022/148‑1 (MQ=255)
                                                              gCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCt                                                                                   <  1:1954653/148‑1 (MQ=255)
                                                              gCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCt                                                                                   <  1:3299849/148‑1 (MQ=255)
                                                              gCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCt                                                                                   >  1:752764/1‑148 (MQ=255)
                                                              gCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCt                                                                                   <  2:942065/148‑1 (MQ=255)
                                                               ccTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCtt                                                                                  >  1:1652177/1‑148 (MQ=255)
                                                               ccTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCtt                                                                                  >  1:948840/1‑148 (MQ=255)
                                                               ccTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCtt                                                                                  >  1:3146683/1‑148 (MQ=255)
                                                                cTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTa                                                                                 >  1:46944/1‑148 (MQ=255)
                                                                 ttGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAg                                                                                >  1:845169/1‑148 (MQ=255)
                                                                 ttGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAg                                                                                >  1:2438808/1‑148 (MQ=255)
                                                                  tGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGc                                                                               <  1:291298/148‑1 (MQ=255)
                                                                  tGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGc                                                                               <  2:3120433/148‑1 (MQ=255)
                                                                   gAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGcc                                                                              >  1:2231849/1‑148 (MQ=255)
                                                                   gAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGcc                                                                              <  1:409370/148‑1 (MQ=255)
                                                                      cAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATa                                                                           >  1:596846/1‑148 (MQ=255)
                                                                      cAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATa                                                                           >  1:3457815/1‑148 (MQ=255)
                                                                       aGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaa                                                                          >  1:48766/1‑148 (MQ=255)
                                                                        ggCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaa                                                                         <  2:1048405/148‑1 (MQ=255)
                                                                         gCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaaa                                                                        >  2:1355886/1‑148 (MQ=255)
                                                                         gCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaaa                                                                        <  2:212576/148‑1 (MQ=255)
                                                                         gCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaaa                                                                        <  1:2323192/148‑1 (MQ=255)
                                                                           ccAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAAtt                                                                      <  2:2697517/148‑1 (MQ=255)
                                                                            cAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTa                                                                     <  2:3002862/148‑1 (MQ=255)
                                                                            cAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTa                                                                     <  2:2906705/148‑1 (MQ=255)
                                                                                 cTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtgtg                                                                <  2:299984/148‑1 (MQ=255)
                                                                                  ttCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGa                                                               <  2:1903837/148‑1 (MQ=255)
                                                                                    cGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAtt                                                             <  1:2988376/148‑1 (MQ=255)
                                                                                      ccAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTg                                                           >  2:1109469/1‑148 (MQ=255)
                                                                                       cAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGc                                                          >  2:2410500/1‑148 (MQ=255)
                                                                                       cAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGc                                                          >  2:627398/1‑148 (MQ=255)
                                                                                        aaTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCg                                                         <  2:72876/148‑1 (MQ=255)
                                                                                         aTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCga                                                        >  2:2487064/1‑148 (MQ=255)
                                                                                             tttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaaa                                                    >  2:3069735/1‑148 (MQ=255)
                                                                                             tttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaaa                                                    <  1:1553269/148‑1 (MQ=255)
                                                                                             tttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaaa                                                    >  2:786582/1‑148 (MQ=255)
                                                                                             tttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaaa                                                    <  1:914895/148‑1 (MQ=255)
                                                                                                ttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCg                                                 <  2:1933446/148‑1 (MQ=255)
                                                                                                         gCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCg                                        <  2:1560634/148‑1 (MQ=255)
                                                                                                            tCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAAt                                     <  2:3545798/148‑1 (MQ=255)
                                                                                                                tttACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTgg                                 <  2:435403/148‑1 (MQ=255)
                                                                                                                tttACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTgg                                 <  2:2276287/148‑1 (MQ=255)
                                                                                                                tttACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTgg                                 >  1:1468282/1‑148 (MQ=255)
                                                                                                                tttACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTgg                                 >  1:2301357/1‑148 (MQ=255)
                                                                                                                 ttACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCAGAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGc                                >  1:2163698/1‑148 (MQ=255)
                                                                                                                    ccTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGa                             >  1:903103/1‑148 (MQ=255)
                                                                                                                    ccTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGa                             >  1:2494953/1‑148 (MQ=255)
                                                                                                                     cTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGaa                            >  1:1760341/1‑148 (MQ=255)
                                                                                                                       cGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGt                          <  1:3431213/148‑1 (MQ=255)
                                                                                                                         cTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTgg                        <  2:900438/148‑1 (MQ=255)
                                                                                                                           ggAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAt                      >  2:2535581/1‑148 (MQ=255)
                                                                                                                           ggAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAt                      >  2:101615/1‑148 (MQ=255)
                                                                                                                               gCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATgccg                  >  2:1152820/1‑148 (MQ=255)
                                                                                                                                  aTGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCa               <  1:1192678/148‑1 (MQ=255)
                                                                                                                                   tGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt              >  1:118265/1‑148 (MQ=255)
                                                                                                                                       ggAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGcc          <  1:1227112/148‑1 (MQ=255)
                                                                                                                                        gAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCt         >  1:3385181/1‑148 (MQ=255)
                                                                                                                                             cGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTgcg    >  2:1450196/1‑148 (MQ=255)
                                                                                                                                               aTGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGcgcg  <  1:2213974/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCG  >  CP009273/3230711‑3231001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑