Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,234,522 T→A 41.0% L407Q (CTG→CAG)  sstT → sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,234,5220TA41.0% 101.4 / 226.0 171L407Q (CTG→CAG) sstTsodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (47/54);  new base A (37/33);  total (84/87)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

Only 100 of 176 total aligned reads displayed.

TGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACC  >  CP009273/3234375‑3234668
                                                                                                                                                   |                                                                                                                                                  
tGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCt                                                                                                                                                    <  1:3445591/148‑1 (MQ=255)
tGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCt                                                                                                                                                    <  1:2934022/148‑1 (MQ=255)
tGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCt                                                                                                                                                    <  1:2934010/148‑1 (MQ=255)
   tCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGc                                                                                                                                                 <  1:1223730/148‑1 (MQ=255)
    cGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCa                                                                                                                                                >  1:2023667/1‑148 (MQ=255)
    cGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCa                                                                                                                                                >  1:2023646/1‑148 (MQ=255)
      gTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCaaa                                                                                                                                              <  1:592712/148‑1 (MQ=255)
      gTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCaaa                                                                                                                                              >  2:1512106/1‑148 (MQ=255)
      gTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCaaa                                                                                                                                              >  2:3276059/1‑148 (MQ=255)
       tATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAAt                                                                                                                                             >  1:934268/1‑148 (MQ=255)
             gAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGcc                                                                                                                                       >  2:22574/1‑148 (MQ=255)
              aaCGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGccc                                                                                                                                      >  1:2187578/1‑148 (MQ=255)
               aCGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCt                                                                                                                                     >  1:980273/1‑148 (MQ=255)
                cGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTg                                                                                                                                    >  2:643818/1‑148 (MQ=255)
                   tatCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGt                                                                                                                                 >  1:475805/1‑148 (MQ=255)
                   tatCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGt                                                                                                                                 >  1:694735/1‑148 (MQ=255)
                    atCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGta                                                                                                                                >  2:3409983/1‑148 (MQ=255)
                      cGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaat                                                                                                                              >  2:2452798/1‑148 (MQ=255)
                      cGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGtaat                                                                                                                              <  1:658590/148‑1 (MQ=255)
                        ccATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaatta                                                                                                                            <  1:1502742/148‑1 (MQ=255)
                        ccATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaatta                                                                                                                            <  1:1501852/148‑1 (MQ=255)
                        ccATGCAGGTGGGTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGtaatta                                                                                                                            <  2:3167411/148‑1 (MQ=255)
                           tGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTaattaatt                                                                                                                         >  1:2870302/1‑148 (MQ=255)
                             cAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGt                                                                                                                       <  1:3490597/148‑1 (MQ=255)
                                     tGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAAcccc                                                                                                               >  1:446737/1‑148 (MQ=255)
                                      gCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCt                                                                                                              <  2:2045322/148‑1 (MQ=255)
                                         gTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTc                                                                                                           >  2:2156487/1‑148 (MQ=255)
                                          tCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCg                                                                                                          >  1:1399280/1‑148 (MQ=255)
                                          tCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCg                                                                                                          >  1:1009159/1‑148 (MQ=255)
                                           cGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGt                                                                                                         <  2:2913086/148‑1 (MQ=255)
                                           cGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGt                                                                                                         >  2:2697000/1‑148 (MQ=255)
                                              cTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTa                                                                                                      >  1:2495955/1‑148 (MQ=255)
                                                 tATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcgg                                                                                                   >  1:677048/1‑148 (MQ=255)
                                                   tcatcGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcggcg                                                                                                 >  1:742343/1‑148 (MQ=255)
                                                   tcatcGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcggcg                                                                                                 <  2:455751/148‑1 (MQ=255)
                                                     atcGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGa                                                                                               >  1:108656/1‑148 (MQ=255)
                                                         gCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAggg                                                                                           >  1:3031293/1‑148 (MQ=255)
                                                          cGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAgggg                                                                                          <  2:279145/148‑1 (MQ=255)
                                                          cGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAgggg                                                                                          <  1:402989/148‑1 (MQ=255)
                                                          cGTATTGCAGGACTCGTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATCAATTGTTTAACCCCTTTCGTCTACGGCGGAAgggg                                                                                          <  2:2118112/148‑1 (MQ=255)
                                                             aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGttt                                                                                       >  1:1147924/1‑148 (MQ=255)
                                                              ttGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGtttt                                                                                      <  1:3192564/148‑1 (MQ=255)
                                                                   ggACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACTATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCaa                                                                                 <  2:2766609/148‑1 (MQ=255)
                                                                     aCTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACt                                                                               <  2:792508/148‑1 (MQ=255)
                                                                     aCTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACt                                                                               >  1:2648208/1‑148 (MQ=255)
                                                                      ctctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACtt                                                                              >  1:1593740/1‑148 (MQ=255)
                                                                         ttGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTaa                                                                           <  2:2583642/148‑1 (MQ=255)
                                                                         ttGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTaa                                                                           <  2:2640286/148‑1 (MQ=255)
                                                                         ttGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTaa                                                                           <  1:132964/148‑1 (MQ=255)
                                                                           gCGAAACCGCGCTGAACTCTTCATCTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTTGCAAATAGGGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCTGAAGGGGTTTTCTCAACTTTAAAc                                                                         <  1:1899922/148‑1 (MQ=255)
                                                                           gCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAAc                                                                         >  2:63729/1‑148 (MQ=255)
                                                                           gCGAAACCGCGCTGAACTCTTCAACGGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAAc                                                                         <  2:3164058/148‑1 (MQ=255)
                                                                             gAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACgg                                                                       <  1:2473868/148‑1 (MQ=255)
                                                                             gAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACgg                                                                       <  2:1704467/148‑1 (MQ=255)
                                                                              aaaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGa                                                                      <  1:2508411/148‑1 (MQ=255)
                                                                               aaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGAt                                                                     >  2:3512359/1‑148 (MQ=255)
                                                                                 ccGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCa                                                                   <  2:3242642/148‑1 (MQ=255)
                                                                                  cgcgCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCaa                                                                  >  2:2183503/1‑148 (MQ=255)
                                                                                        gAACTCGTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTcccc                                                            <  2:1965607/148‑1 (MQ=255)
                                                                                         aaCTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCt                                                           <  2:1040130/148‑1 (MQ=255)
                                                                                          aCTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCtt                                                          >  2:920926/1‑148 (MQ=255)
                                                                                           ctctTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCttt                                                         >  1:1555495/1‑148 (MQ=255)
                                                                                            tctTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCtttt                                                        <  2:1170609/148‑1 (MQ=255)
                                                                                                 aaCTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGTTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCa                                                   <  2:3548336/148‑1 (MQ=255)
                                                                                                  aCTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCAt                                                  <  1:959790/148‑1 (MQ=255)
                                                                                                      aCGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGc                                              <  2:836567/148‑1 (MQ=255)
                                                                                                      aCGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAAGTGTTTAACCCCTTTCGTCTCCGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGc                                              <  2:3154004/148‑1 (MQ=255)
                                                                                                      aCGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGc                                              >  1:2726420/1‑148 (MQ=255)
                                                                                                      aCGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGc                                              >  1:3387666/1‑148 (MQ=255)
                                                                                                          gCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGa                                          >  2:586110/1‑148 (MQ=255)
                                                                                                          gCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGa                                          >  1:3538526/1‑148 (MQ=255)
                                                                                                          gCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGa                                          >  2:2417999/1‑148 (MQ=255)
                                                                                                            tGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGaaa                                        <  1:1045282/148‑1 (MQ=255)
                                                                                                              ttCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACg                                      >  1:411640/1‑148 (MQ=255)
                                                                                                               tCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGa                                     >  2:3102146/1‑148 (MQ=255)
                                                                                                               tCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGa                                     >  1:2602069/1‑148 (MQ=255)
                                                                                                               tCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGa                                     >  1:1725881/1‑148 (MQ=255)
                                                                                                               tCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGa                                     >  1:1725231/1‑148 (MQ=255)
                                                                                                                cACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGaa                                    <  2:2870279/148‑1 (MQ=255)
                                                                                                                cACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGaa                                    <  2:2870302/148‑1 (MQ=255)
                                                                                                                cACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGaa                                    <  2:1358917/148‑1 (MQ=255)
                                                                                                                cACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGaa                                    <  2:589236/148‑1 (MQ=255)
                                                                                                                  cTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATg                                  <  1:2269944/148‑1 (MQ=255)
                                                                                                                  cTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATg                                  <  1:2664154/148‑1 (MQ=255)
                                                                                                                     cGGCAGCTTGCCAGGCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGata                               >  2:1150085/1‑148 (MQ=255)
                                                                                                                                 aGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTc                   >  2:640981/1‑148 (MQ=255)
                                                                                                                                  ggCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCa                  >  1:194633/1‑148 (MQ=255)
                                                                                                                                  ggCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCa                  >  2:611448/1‑148 (MQ=255)
                                                                                                                                  ggCAGAAGACGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCa                  <  2:1093404/148‑1 (MQ=255)
                                                                                                                                   gCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCAt                 >  1:1482354/1‑148 (MQ=255)
                                                                                                                                      gaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAATCGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAg              >  1:945854/1‑148 (MQ=255)
                                                                                                                                        agaCGATCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCa            >  1:2646192/1‑148 (MQ=255)
                                                                                                                                          aCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGa          >  1:300957/1‑148 (MQ=255)
                                                                                                                                             aTCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa       <  2:529183/148‑1 (MQ=255)
                                                                                                                                             aTCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa       <  2:363063/148‑1 (MQ=255)
                                                                                                                                             aTCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa       <  2:3416042/148‑1 (MQ=255)
                                                                                                                                             aTCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa       <  2:1656858/148‑1 (MQ=255)
                                                                                                                                              tCGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAAc      >  2:2522205/1‑148 (MQ=255)
                                                                                                                                               cGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACt     >  1:3126209/1‑148 (MQ=255)
                                                                                                                                               cGTCAGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACt     <  2:2023646/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACC  >  CP009273/3234375‑3234668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑