Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,582,807 G→T 100% P194Q (CCG→CAG)  ugpE ← glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,582,8070GT99.2% 840.1 / ‑2.2 256P194Q (CCG→CAG) ugpEglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (135/119);  total (137/119)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

TTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582662‑3582951
                                                                                                                                                 |                                                                                                                                                
ttCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGa                                                                                                                                                >  2:1054411/1‑148 (MQ=255)
   gCCTGTAGCTATCAGCCCTTTTATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaa                                                                                                                                             <  1:2730459/148‑1 (MQ=255)
   gCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaa                                                                                                                                             <  2:1416482/148‑1 (MQ=255)
    ccTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaaa                                                                                                                                            >  1:3323171/1‑148 (MQ=255)
      tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACCACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACg                                                                                                                                          <  2:2584568/148‑1 (MQ=255)
      tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACg                                                                                                                                          >  1:905953/1‑148 (MQ=255)
       gTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGa                                                                                                                                         >  2:3141461/1‑148 (MQ=255)
        tAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGAt                                                                                                                                        <  2:1615581/148‑1 (MQ=255)
                atcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaaa                                                                                                                                >  1:1902712/1‑148 (MQ=255)
                  cccTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaag                                                                                                                              >  2:31161/1‑148 (MQ=255)
                    cTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaa                                                                                                                            <  2:793833/148‑1 (MQ=255)
                     tttGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaag                                                                                                                           >  2:1726832/1‑148 (MQ=255)
                     tttGAGCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACACCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaag                                                                                                                           <  2:790532/148‑1 (MQ=255)
                       tGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAgcg                                                                                                                         <  2:229125/148‑1 (MQ=255)
                          tCCCTGCCACGGTGGTGCCGAGATCCACATCGTTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                      <  1:2429624/148‑1 (MQ=255)
                          tCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                      >  2:299536/1‑148 (MQ=255)
                          tCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                      >  1:1536881/1‑148 (MQ=255)
                           cccTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAtt                                                                                                                     >  1:487356/1‑148 (MQ=255)
                           cccTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAtt                                                                                                                     >  2:508242/1‑148 (MQ=255)
                            ccTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTg                                                                                                                    >  1:3473188/1‑148 (MQ=255)
                               gCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCg                                                                                                                 <  2:2081962/148‑1 (MQ=255)
                               gCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCg                                                                                                                 <  2:2447772/148‑1 (MQ=255)
                                ccACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTATTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGa                                                                                                                <  2:2392868/148‑1 (MQ=255)
                                  aCGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATg                                                                                                              >  1:2616775/1‑148 (MQ=255)
                                      tggtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGcc                                                                                                          >  2:1147082/1‑148 (MQ=255)
                                         tgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTc                                                                                                       >  1:622889/1‑148 (MQ=255)
                                             gataTCAACATCGGTAATAATCACCAACGGCCACAAATACTGATTCCAGCCGTATATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATc                                                                                                   <  1:3094537/145‑1 (MQ=255)
                                             gagaTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATc                                                                                                   <  2:2612864/148‑1 (MQ=255)
                                                aTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTATTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgc                                                                                                >  1:1857664/1‑148 (MQ=255)
                                                 tCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               >  1:3561196/1‑148 (MQ=255)
                                                  ccACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcgc                                                                                              >  1:1421814/1‑148 (MQ=255)
                                                    acaTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCgg                                                                                            >  1:1978059/1‑148 (MQ=255)
                                                     caTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGc                                                                                           >  1:2237347/1‑148 (MQ=255)
                                                     caTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGc                                                                                           >  1:3123422/1‑148 (MQ=255)
                                                       tCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCtt                                                                                         >  1:1593541/1‑148 (MQ=255)
                                                           taataaTCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTccac                                                                                     <  2:3535601/148‑1 (MQ=255)
                                                              taatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAg                                                                                  <  1:3198999/148‑1 (MQ=255)
                                                               aatCAACACCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGc                                                                                 <  1:3035788/148‑1 (MQ=255)
                                                               aatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGc                                                                                 >  2:3251145/1‑148 (MQ=255)
                                                                atCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCt                                                                                >  2:2728056/1‑148 (MQ=255)
                                                                  caacaaCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCa                                                                              <  2:1090862/148‑1 (MQ=255)
                                                                  caacaaCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCa                                                                              >  1:3411030/1‑148 (MQ=255)
                                                                    acaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATc                                                                            >  1:2938946/1‑148 (MQ=255)
                                                                     caacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATcc                                                                           >  2:654861/1‑148 (MQ=255)
                                                                     caacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATcc                                                                           <  2:1166548/148‑1 (MQ=255)
                                                                       acGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCgg                                                                         >  2:519705/1‑148 (MQ=255)
                                                                         ggCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCa                                                                       <  1:12295/148‑1 (MQ=255)
                                                                            cacaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCg                                                                    <  2:2177768/148‑1 (MQ=255)
                                                                             acaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                   >  2:135931/1‑148 (MQ=255)
                                                                             acaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                   >  1:2572501/1‑148 (MQ=255)
                                                                              caAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTc                                                                  >  1:1881594/1‑148 (MQ=255)
                                                                              caAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTc                                                                  <  1:1818109/148‑1 (MQ=255)
                                                                               aaaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCa                                                                 >  2:713336/1‑148 (MQ=255)
                                                                                 aTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATa                                                               >  1:2702651/1‑148 (MQ=255)
                                                                                  tACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaa                                                              <  2:3398439/148‑1 (MQ=255)
                                                                                  tACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaa                                                              <  2:872978/148‑1 (MQ=255)
                                                                                    cTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAg                                                            >  1:136546/1‑148 (MQ=255)
                                                                                     tGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGa                                                           <  1:1054411/148‑1 (MQ=255)
                                                                                      gagtCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          <  2:1966332/145‑1 (MQ=255)
                                                                                      gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          <  1:1064869/148‑1 (MQ=255)
                                                                                      gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          >  1:2125642/1‑148 (MQ=255)
                                                                                      gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          >  1:2127237/1‑148 (MQ=255)
                                                                                       aTTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAAc                                                         <  1:401741/148‑1 (MQ=255)
                                                                                         tCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTg                                                       >  2:1671100/1‑148 (MQ=255)
                                                                                         tCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTg                                                       <  1:2191658/148‑1 (MQ=255)
                                                                                         tCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTg                                                       <  2:278866/148‑1 (MQ=255)
                                                                                                gTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAAc                                                >  1:2920120/1‑148 (MQ=255)
                                                                                                  aGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAg                                              >  2:1486094/1‑148 (MQ=255)
                                                                                                  aGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAg                                              >  2:407863/1‑148 (MQ=255)
                                                                                                  aGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAg                                              <  2:1209766/148‑1 (MQ=255)
                                                                                                   gATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAgg                                             >  1:2345648/1‑148 (MQ=255)
                                                                                                    aTAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGa                                            >  1:50004/1‑148 (MQ=255)
                                                                                                     tAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGaa                                           <  2:1081411/148‑1 (MQ=255)
                                                                                                        aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                        >  1:1628209/1‑148 (MQ=255)
                                                                                                        aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                        <  2:3551405/148‑1 (MQ=255)
                                                                                                        aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                        >  1:738525/1‑148 (MQ=255)
                                                                                                        aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                        >  1:264472/1‑148 (MQ=255)
                                                                                                         ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                       <  2:3062864/148‑1 (MQ=255)
                                                                                                          gTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAg                                      <  1:169886/148‑1 (MQ=255)
                                                                                                          gTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAg                                      >  1:762992/1‑148 (MQ=255)
                                                                                                             aTCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCgg                                   <  2:2127237/148‑1 (MQ=255)
                                                                                                             aTCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCgg                                   <  2:2924586/148‑1 (MQ=255)
                                                                                                                    aCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGa                            >  1:188810/1‑148 (MQ=255)
                                                                                                                       gcgcCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGc                         <  2:1839098/148‑1 (MQ=255)
                                                                                                                          ccgccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCAt                      <  1:1925919/148‑1 (MQ=255)
                                                                                                                            gccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCa                    <  2:1009119/148‑1 (MQ=255)
                                                                                                                             ccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAg                   >  1:3110692/1‑148 (MQ=255)
                                                                                                                               aGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCg                 >  2:260489/1‑148 (MQ=255)
                                                                                                                                      ttttGGAGAGCTTAATAACGATGTCGCAAAAGAAGCGCTTTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          <  1:1752196/148‑1 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          >  2:2017851/1‑148 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          <  2:1946856/148‑1 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          >  1:3176968/1‑148 (MQ=255)
                                                                                                                                       tttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGtt         <  2:13248/148‑1 (MQ=255)
                                                                                                                                       tttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGtt         <  2:13035/148‑1 (MQ=255)
                                                                                                                                        ttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTa        <  2:2256683/148‑1 (MQ=255)
                                                                                                                                          ggAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTaaa      <  2:1868132/148‑1 (MQ=255)
                                                                                                                                              agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  >  2:1509145/1‑148 (MQ=255)
                                                                                                                                              agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  >  1:3011278/1‑148 (MQ=255)
                                                                                                                                              agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  >  1:3174288/1‑148 (MQ=255)
                                                                                                                                              agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  <  1:1697409/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582662‑3582951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑