Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,607,110 C→A 100% I28I (ATC→ATA)  nikA → nickel‑binding, heme‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,607,1100CA96.0% 604.6 / 14.8 199I28I (ATC→ATA) nikAnickel‑binding, heme‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base A (92/99);  total (98/101)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 215 total aligned reads displayed.

TGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACT  >  CP009273/3606965‑3607257
                                                                                                                                                 |                                                                                                                                                   
tGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaac                                                                                                                                                   >  2:1007215/1‑148 (MQ=255)
 gACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacc                                                                                                                                                  >  2:2204297/1‑148 (MQ=255)
  aCGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                                 <  1:1055528/148‑1 (MQ=255)
  aCGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                                 <  2:1745789/148‑1 (MQ=255)
  aCGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                                 <  1:2833110/148‑1 (MQ=255)
   cGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaaccac                                                                                                                                                >  2:1912368/1‑148 (MQ=255)
   cGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaaccac                                                                                                                                                <  2:1389707/148‑1 (MQ=255)
   cGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaaccac                                                                                                                                                <  1:1511610/148‑1 (MQ=255)
    gAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAaccacc                                                                                                                                               <  1:2332433/148‑1 (MQ=255)
      aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGc                                                                                                                                             <  1:184844/148‑1 (MQ=255)
       tACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                            >  2:1457398/1‑148 (MQ=255)
       tACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                            <  1:933201/148‑1 (MQ=255)
        aCTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCt                                                                                                                                           >  2:819602/1‑148 (MQ=255)
        aCTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCt                                                                                                                                           >  2:2604795/1‑148 (MQ=255)
          ttAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTgg                                                                                                                                         <  1:3481793/148‑1 (MQ=255)
              aaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCgg                                                                                                                                     >  2:1581987/1‑148 (MQ=255)
               aTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                    >  1:1266830/1‑148 (MQ=255)
               aTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                    <  1:1332940/148‑1 (MQ=255)
               aTCGTCAGACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                    <  2:3331147/148‑1 (MQ=255)
                   tCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAAt                                                                                                                                >  1:72974/1‑148 (MQ=255)
                     aTACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGt                                                                                                                              >  1:1756315/1‑148 (MQ=255)
                      tACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTc                                                                                                                             >  1:122036/1‑148 (MQ=255)
                      tACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTc                                                                                                                             >  1:3094762/1‑148 (MQ=255)
                       aCTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCg                                                                                                                            >  2:2870848/1‑148 (MQ=255)
                       aCTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCg                                                                                                                            >  1:2075725/1‑148 (MQ=255)
                         ttATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCggg                                                                                                                          >  1:1577283/1‑148 (MQ=255)
                         ttATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCggg                                                                                                                          <  1:1972460/148‑1 (MQ=255)
                          tATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                         >  1:1398113/1‑148 (MQ=255)
                            tttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCa                                                                                                                       >  2:2318526/1‑148 (MQ=255)
                                 gCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTaaa                                                                                                                  <  1:804361/148‑1 (MQ=255)
                                 gCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTaaa                                                                                                                  <  2:2776972/148‑1 (MQ=255)
                                 gCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTaaa                                                                                                                  <  2:400064/148‑1 (MQ=255)
                                  ccATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAc                                                                                                                 >  1:2915138/1‑148 (MQ=255)
                                   cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAcc                                                                                                                >  2:604353/1‑148 (MQ=255)
                                     tCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCg                                                                                                              >  2:2278572/1‑148 (MQ=255)
                                     tCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCg                                                                                                              >  2:1802962/1‑148 (MQ=255)
                                      cTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGc                                                                                                             >  1:3241108/1‑148 (MQ=255)
                                        aTTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                           >  1:2548153/1‑148 (MQ=255)
                                         ttttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAcc                                                                                                          <  1:3533329/148‑1 (MQ=255)
                                         ttttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAcc                                                                                                          <  2:2439384/148‑1 (MQ=255)
                                          tttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCt                                                                                                         >  1:944486/1‑148 (MQ=255)
                                            tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                       >  1:734249/1‑148 (MQ=255)
                                            tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                       <  2:2934501/148‑1 (MQ=255)
                                                ccATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTacac                                                                                                   >  1:39994/1‑148 (MQ=255)
                                                  aTTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGc                                                                                                 >  1:1537319/1‑148 (MQ=255)
                                                    tGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCt                                                                                               >  1:3055040/1‑148 (MQ=255)
                                                     ggggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTa                                                                                              <  1:2736185/148‑1 (MQ=255)
                                                      gggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTaa                                                                                             <  1:1607750/148‑1 (MQ=255)
                                                       ggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAAc                                                                                            >  1:1747510/1‑148 (MQ=255)
                                                        gTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAAcc                                                                                           <  2:1540433/148‑1 (MQ=255)
                                                          tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAg                                                                                         >  2:3039028/1‑148 (MQ=255)
                                                          tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAg                                                                                         >  1:1542637/1‑148 (MQ=255)
                                                          tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAg                                                                                         <  1:1085008/148‑1 (MQ=255)
                                                           aCCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGa                                                                                        >  1:1139511/1‑148 (MQ=255)
                                                              aTGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGt                                                                                     <  2:1263776/148‑1 (MQ=255)
                                                                gCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTc                                                                                   >  2:2886895/1‑148 (MQ=255)
                                                                    tcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGccc                                                                               >  2:3230479/1‑148 (MQ=255)
                                                                    tcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGccc                                                                               >  2:2357853/1‑148 (MQ=255)
                                                                      cacaCTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCag                                                                             <  1:2170318/148‑1 (MQ=255)
                                                                        cacTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGACTGTCGGGCCCCTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCcgag                                                                           >  2:507667/1‑148 (MQ=255)
                                                                        cacTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCagag                                                                           <  2:1673462/148‑1 (MQ=255)
                                                                        cacTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCagag                                                                           <  2:1277499/148‑1 (MQ=255)
                                                                         acTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGc                                                                          <  1:3019964/148‑1 (MQ=255)
                                                                          cTCAGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                         >  2:1360462/1‑148 (MQ=255)
                                                                            ccgccgCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATg                                                                       <  2:1715415/148‑1 (MQ=255)
                                                                            ccgccgCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATg                                                                       <  2:3479622/148‑1 (MQ=255)
                                                                            ccgccgCACTCGATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATg                                                                       <  2:3479701/148‑1 (MQ=255)
                                                                              gccgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGt                                                                     <  2:2020446/148‑1 (MQ=255)
                                                                                 gcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTa                                                                  <  2:2014122/148‑1 (MQ=255)
                                                                                       tttttGGGCTGCGGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCAT‑GTTTATGAACCa                                                           <  2:370301/146‑1 (MQ=255)
                                                                                        aTTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCa                                                           <  1:1793922/148‑1 (MQ=255)
                                                                                         tttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCATAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAt                                                          <  2:2504552/148‑1 (MQ=255)
                                                                                                  ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAat                                                 <  1:2218950/148‑1 (MQ=255)
                                                                                                  ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAat                                                 <  2:2287000/148‑1 (MQ=255)
                                                                                                  ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAat                                                 <  2:2618765/148‑1 (MQ=255)
                                                                                                   tgGCTTGTGAGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAata                                                <  1:2088238/148‑1 (MQ=255)
                                                                                                     gCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                              >  1:427456/1‑148 (MQ=255)
                                                                                                        tgtgCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAgg                                           >  1:2154560/1‑148 (MQ=255)
                                                                                                        tgtgCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAgg                                           >  2:700165/1‑148 (MQ=255)
                                                                                                           gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCTCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAg                                        >  1:344369/1‑148 (MQ=255)
                                                                                                           gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCTCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAg                                        >  1:344310/1‑148 (MQ=255)
                                                                                                           gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAg                                        >  1:1903778/1‑148 (MQ=255)
                                                                                                           gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAg                                        >  2:615071/1‑148 (MQ=255)
                                                                                                           gCGTCTTTTATCGTCAATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGAGGTTCTCCCAGAGCATGGTTTATGAACCATTGGTGAACTATCAGGCAg                                        >  1:2768730/1‑148 (MQ=255)
                                                                                                            cGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGa                                       <  1:163635/148‑1 (MQ=255)
                                                                                                              tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACg                                     >  1:2601240/1‑148 (MQ=255)
                                                                                                              tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACg                                     <  2:3126334/148‑1 (MQ=255)
                                                                                                              tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACg                                     <  2:66003/148‑1 (MQ=255)
                                                                                                              tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACg                                     >  1:3145467/1‑148 (MQ=255)
                                                                                                              tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACg                                     <  2:516150/148‑1 (MQ=255)
                                                                                                                 tttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGtt                                  <  1:2604795/148‑1 (MQ=255)
                                                                                                                    aTCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAACCCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCgg                               <  2:2226700/148‑1 (MQ=255)
                                                                                                                     tCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                              <  2:1790650/148‑1 (MQ=255)
                                                                                                                     tCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                              <  1:1248118/148‑1 (MQ=255)
                                                                                                                          cccgCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATcc                         <  1:2710561/145‑1 (MQ=255)
                                                                                                                                gCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggc                   >  2:714121/1‑148 (MQ=255)
                                                                                                                                   gCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctgg                >  2:3431799/1‑148 (MQ=255)
                                                                                                                                           tGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGc        >  1:3405733/1‑148 (MQ=255)
                                                                                                                                              aaTAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTgg     >  1:1737046/1‑148 (MQ=255)
                                                                                                                                              aaTAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTgg     >  2:1191900/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACT  >  CP009273/3606965‑3607257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑