Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,616,714 T→G 20.5% I1388R (ATA→AGA)  rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,616,7140TG20.5% 506.3 / 171.5 291I1388R (ATA→AGA) rhsBRhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (128/103);  new base G (33/27);  total (161/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

TATCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCAGTGTAATA  >  CP009273/3616567‑3616861
                                                                                                                                                   |                                                                                                                                                   
  tCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAg                                                                                                                                                   <  2:3070881/148‑1 (MQ=255)
  tCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAg                                                                                                                                                   <  1:2996103/148‑1 (MQ=255)
   cAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGa                                                                                                                                                  >  1:1474805/1‑148 (MQ=255)
    aGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGAt                                                                                                                                                 >  1:3565861/1‑148 (MQ=255)
    aGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGAt                                                                                                                                                 <  1:737991/148‑1 (MQ=255)
    aGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGAt                                                                                                                                                 <  1:1580093/148‑1 (MQ=255)
    aGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGAt                                                                                                                                                 <  2:477203/148‑1 (MQ=255)
     gCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATa                                                                                                                                                >  2:1825612/1‑148 (MQ=255)
        tATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAAcc                                                                                                                                             <  2:147305/148‑1 (MQ=255)
         aTCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCa                                                                                                                                            >  2:69684/1‑148 (MQ=255)
                tCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGAAAGAGATAACCACGggaag                                                                                                                                     >  2:2792499/1‑148 (MQ=255)
                 cATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGggaagg                                                                                                                                    >  2:861206/1‑148 (MQ=255)
                 cATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGggaagg                                                                                                                                    <  1:450211/148‑1 (MQ=255)
                 cATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGggaagg                                                                                                                                    >  2:554337/1‑148 (MQ=255)
                  aTGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGggaagga                                                                                                                                   <  1:3495856/148‑1 (MQ=255)
                    ggTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGaaa                                                                                                                                 <  2:1101425/148‑1 (MQ=255)
                           aTAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATaacaaa                                                                                                                          <  2:1304350/148‑1 (MQ=255)
                           aTAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATaacaaa                                                                                                                          <  1:1262513/148‑1 (MQ=255)
                            tAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATaacaaac                                                                                                                         >  1:1079864/1‑148 (MQ=255)
                            tAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATaacaaac                                                                                                                         >  2:1968961/1‑148 (MQ=255)
                               tCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGa                                                                                                                      >  2:429492/1‑148 (MQ=255)
                                cATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGaa                                                                                                                     <  1:3230627/148‑1 (MQ=255)
                                   ggAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAg                                                                                                                  <  2:2409427/148‑1 (MQ=255)
                                   ggAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAg                                                                                                                  <  2:2565320/148‑1 (MQ=255)
                                     agaATATTGTGACTAAATATTTTCAGGTATAGATGAGAAAATAGTTAAAATTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGa                                                                                                                <  1:2394423/148‑1 (MQ=255)
                                     agaATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGa                                                                                                                >  2:2279626/1‑148 (MQ=255)
                                      gaATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGAt                                                                                                               >  1:2281406/1‑148 (MQ=255)
                                       aaTATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGAtt                                                                                                              >  2:1337145/1‑148 (MQ=255)
                                            tGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAATTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACATAAAAGGATTACCTa                                                                                                         >  2:2987505/1‑148 (MQ=255)
                                              ggACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAAt                                                                                                       >  2:2171700/1‑148 (MQ=255)
                                                  gAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCt                                                                                                   >  1:756/1‑148 (MQ=255)
                                                   aaaTATTTTCAGGAAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTg                                                                                                  >  1:1974355/1‑148 (MQ=255)
                                                     atatTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCa                                                                                                >  1:142029/1‑148 (MQ=255)
                                                         tttCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAACGGATTACCTAATGTCTGCAGAGt                                                                                            >  2:3459306/1‑148 (MQ=255)
                                                            cAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCa                                                                                         >  2:1530242/1‑148 (MQ=255)
                                                             aGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCAt                                                                                        <  2:1465774/148‑1 (MQ=255)
                                                                tAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGt                                                                                     >  2:1513136/1‑148 (MQ=255)
                                                                 caaGATCAGAAAATACTTAACAGTAAAGAAAACAATAACCCGTCATTTTATTTAGGTACGCCCAGCGGTAATTTTAAAGCAATCCATAACCACGGGAATTAAATAACAAACAGAAAATGATTACCTAATTTCTGCAGAGTTCATGGTa                                                                                    <  2:3268454/147‑1 (MQ=255)
                                                                 aaaGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTa                                                                                    <  1:140610/148‑1 (MQ=255)
                                                                   aGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAat                                                                                  >  2:1407217/1‑148 (MQ=255)
                                                                   aGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAat                                                                                  <  1:485983/148‑1 (MQ=255)
                                                                    gATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTAcc                                                                                                           <  2:598252/122‑1 (MQ=255)
                                                                    gATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTAcc                                                                                                           >  1:598252/1‑122 (MQ=255)
                                                                    gATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGAAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAata                                                                                 >  2:3483289/1‑148 (MQ=255)
                                                                     aTGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAatat                                                                                <  1:2627867/148‑1 (MQ=255)
                                                                      tGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTTGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATg                                                                               <  1:971306/148‑1 (MQ=255)
                                                                        agaAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTa                                                                             >  1:2344022/1‑148 (MQ=255)
                                                                          aaaaTAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTaaa                                                                           >  1:2074443/1‑148 (MQ=255)
                                                                          aaaaTAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTaaa                                                                           >  2:1169473/1‑148 (MQ=255)
                                                                          aaaaTAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTaaa                                                                           >  1:2212647/1‑148 (MQ=255)
                                                                           aaaTAGTTAAAAGTAAATATAACAATATCAAGACATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGTAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTaaaa                                                                          >  1:595845/1‑148 (MQ=255)
                                                                             aTAGTTAAAAGTAAATATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTaaaaaa                                                                        <  1:856279/148‑1 (MQ=255)
                                                                             aTAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTaaaaaa                                                                        >  1:3456928/1‑148 (MQ=255)
                                                                                gTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAata                                                                     <  1:92816/148‑1 (MQ=255)
                                                                                gTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAata                                                                     <  2:3211897/148‑1 (MQ=255)
                                                                                    aaaGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGt                                                                 <  2:3540718/148‑1 (MQ=255)
                                                                                    aaaGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGt                                                                 >  2:2760871/1‑148 (MQ=255)
                                                                                       gTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTa                                                              <  1:843803/148‑1 (MQ=255)
                                                                                         aaaGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAgg                                                            >  2:2707084/1‑148 (MQ=255)
                                                                                         aaaGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAgg                                                            <  1:3344673/148‑1 (MQ=255)
                                                                                           aGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGaa                                                          <  1:1282227/148‑1 (MQ=255)
                                                                                           aGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGaa                                                          >  1:1441984/1‑148 (MQ=255)
                                                                                           aGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGaa                                                          <  1:1040123/148‑1 (MQ=255)
                                                                                             aTAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAATTATTGTTTAGGAACt                                                        >  2:1371713/1‑148 (MQ=255)
                                                                                             aTAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACt                                                        <  1:524460/148‑1 (MQ=255)
                                                                                              tAACAATATCTAGTCATTTTATTTAGGTACGCCCAGTTGTAATTTTATAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACtg                                                       <  1:212777/148‑1 (MQ=255)
                                                                                              tAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGTAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACtg                                                       <  2:1783138/148‑1 (MQ=255)
                                                                                               aaCAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACtgt                                                      >  2:2730344/1‑148 (MQ=255)
                                                                                                 cAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTgtgt                                                    >  1:2708488/1‑148 (MQ=255)
                                                                                                 cAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTgtgt                                                    >  1:403587/1‑148 (MQ=255)
                                                                                                     atCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCAtt                                                <  2:1737382/148‑1 (MQ=255)
                                                                                                     atCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCAtt                                                <  1:1027423/148‑1 (MQ=255)
                                                                                                     atCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCAtt                                                <  1:3261091/148‑1 (MQ=255)
                                                                                                      tCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTg                                               >  1:1521243/1‑148 (MQ=255)
                                                                                                      tCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTg                                               >  1:1000637/1‑148 (MQ=255)
                                                                                                          gTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATc                                           >  2:2544208/1‑148 (MQ=255)
                                                                                                            cATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCtt                                         >  1:242282/1‑148 (MQ=255)
                                                                                                             aTTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCttt                                        >  1:1103167/1‑148 (MQ=255)
                                                                                                                 tatttaGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCtttgttt                                    <  1:2878826/148‑1 (MQ=255)
                                                                                                                  atttaGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCtttgtttg                                   >  2:413440/1‑148 (MQ=255)
                                                                                                                    ttaGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTtgtttgtt                                 <  1:1697012/148‑1 (MQ=255)
                                                                                                                     taGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTgtttgttt                                <  2:1613699/148‑1 (MQ=255)
                                                                                                                     taGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTgtttgttt                                <  2:3380232/148‑1 (MQ=255)
                                                                                                                       ggTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTACTGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGttttt                              <  1:1337185/148‑1 (MQ=255)
                                                                                                                           cGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTtacat                          <  1:2477702/148‑1 (MQ=255)
                                                                                                                             cccAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATTTTTGTTTGTTTTtacatac                        >  2:2096546/1‑148 (MQ=255)
                                                                                                                                  tGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAacac                   >  1:3248200/1‑148 (MQ=255)
                                                                                                                                  tGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAacac                   <  1:279425/148‑1 (MQ=255)
                                                                                                                                  tGGTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAacac                   >  2:148767/1‑148 (MQ=255)
                                                                                                                                   ggTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAAcaca                  >  1:1947014/1‑148 (MQ=255)
                                                                                                                                    gTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACAt                 <  1:2544208/148‑1 (MQ=255)
                                                                                                                                    gTAATTTTAAAGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACAt                 <  1:1825612/148‑1 (MQ=255)
                                                                                                                                           tAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCa          <  1:1559717/148‑1 (MQ=255)
                                                                                                                                            aaaGCAAGAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCAg         >  2:3080573/1‑148 (MQ=255)
                                                                                                                                              aGCACTAGATCCCCACGGGAAGGAAATAACACACAGACAAGGATTACCTACTGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTGGTTTTTACATACAACACATGGGCGCAgtg       <  1:681641/148‑1 (MQ=255)
                                                                                                                                              aGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCAgtg       <  1:1169473/148‑1 (MQ=255)
                                                                                                                                               gCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCAgtgt      >  2:2196734/1‑148 (MQ=255)
                                                                                                                                                cAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCAGTGta     <  2:638183/148‑1 (MQ=255)
                                                                                                                                                  aTAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATTTTTGTTTGTTTTTACATACAACACATGGGCGCAGTGtaat   >  2:894705/1‑148 (MQ=255)
                                                                                                                                                   tAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCAGTGtaata  <  2:3329945/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TATCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAAAAAAATATTGTTTAGGAACTGTGTCATTGTATCTTTGTTTGTTTTTACATACAACACATGGGCGCAGTGTAATA  >  CP009273/3616567‑3616861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑