BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,618,536	C→A	100%	R267R (CGG→AGG)	yhhI →	putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,618,536	0	C	A	97.0%	642.7 / 9.9	243	R267R (CGG→AGG)	yhhI	putative transposase
Reads supporting (aligned to +/- strand): ref base C (5/3); new base A (127/108); total (132/111)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 243 total aligned reads displayed.

AGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGA  >  CP009273/3618391‑3618678

agagCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAgg                                                                                                                                              >  1:1300147/1‑148 (MQ=21)

 gagCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGt                                                                                                                                             >  2:72834/1‑148 (MQ=18)

 gagCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGt                                                                                                                                             <  1:2231784/148‑1 (MQ=18)

 gagCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGt                                                                                                                                             <  1:1534600/148‑1 (MQ=18)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTc                                                                                                                                            >  2:631731/1‑148 (MQ=32)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTc                                                                                                                                            >  1:40636/1‑148 (MQ=17)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTc                                                                                                                                            >  1:2761998/1‑148 (MQ=17)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTc                                                                                                                                            >  2:3255967/1‑148 (MQ=17)

   gCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTcc                                                                                                                                           <  2:1414516/148‑1 (MQ=17)

     aTGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCat                                                                                                                                         <  1:720349/148‑1 (MQ=17)

      tGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCata                                                                                                                                        >  2:1445525/1‑148 (MQ=17)

          aGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAg                                                                                                                                    <  2:2994042/148‑1 (MQ=17)

            ttACGCAATCAGTGAAAAGAGTCACTGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGAAGTCTCCTTTATGTCCATAATAgaa                                                                                                                                  >  1:2778458/1‑146 (MQ=255)

            ttACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCa                                                                                                                                  >  2:2877123/1‑148 (MQ=17)

               cGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGaa                                                                                                                               <  1:53191/148‑1 (MQ=17)

                  aaTCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACaa                                                                                                                            <  1:374750/148‑1 (MQ=17)

                    tCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACaaaa                                                                                                                          >  1:312992/1‑148 (MQ=14)

                     cAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAaaag                                                                                                                         >  1:2886319/1‑148 (MQ=11)

                     cAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAaaag                                                                                                                         <  1:2824886/148‑1 (MQ=11)

                     cAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAaaag                                                                                                                         >  1:1107992/1‑148 (MQ=11)

                                 tCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGaa                                                                                                             <  1:686759/148‑1 (MQ=18)

                                  cACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGaaa                                                                                                            <  2:1490537/148‑1 (MQ=21)

                                   aCGGCAGAGAAGAAATCCGTCTTCATATTGTTTGAGATGTCACTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAAt                                                                                                           >  1:799448/1‑148 (MQ=255)

                                    cGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATg                                                                                                          <  1:1403083/148‑1 (MQ=21)

                                    cGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATg                                                                                                          <  1:1199644/148‑1 (MQ=21)

                                    cGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATg                                                                                                          <  2:1650744/148‑1 (MQ=21)

                                         gagaAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGt                                                                                                     >  2:2235629/1‑148 (MQ=21)

                                         gagaAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGt                                                                                                     <  1:1483000/148‑1 (MQ=21)

                                          agaagaAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTc                                                                                                    >  1:1635831/1‑148 (MQ=21)

                                            aagaaATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAg                                                                                                  <  1:917916/148‑1 (MQ=21)

                                               aaaTCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGata                                                                                               <  2:895595/148‑1 (MQ=21)

                                               aaaTCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGAAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGACAGAAATGACGGTCAGata                                                                                               >  1:3554033/1‑148 (MQ=255)

                                                 aTCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGAtatt                                                                                             >  2:3114484/1‑148 (MQ=21)

                                                 aTCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGAtatt                                                                                             >  1:2068990/1‑148 (MQ=21)

                                                   ccGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGAtattat                                                                                           <  2:935127/148‑1 (MQ=21)

                                                   ccGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGAtattat                                                                                           <  1:3017744/148‑1 (MQ=21)

                                                    cGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATtata                                                                                          >  2:2683095/1‑148 (MQ=21)

                                                      tCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATc                                                                                        >  2:1136957/1‑148 (MQ=21)

                                                       cTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCa                                                                                       <  1:2030564/148‑1 (MQ=21)

                                                       cTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCa                                                                                       <  1:3111563/148‑1 (MQ=21)

                                                       cTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCa                                                                                       >  2:2730703/1‑148 (MQ=21)

                                                         tCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGt                                                                                     >  2:2974683/1‑148 (MQ=21)

                                                         tCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGt                                                                                     <  1:77614/148‑1 (MQ=21)

                                                         tCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGt                                                                                     <  2:1849894/148‑1 (MQ=21)

                                                          cATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTGTGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGtt                                                                                    <  2:939514/148‑1 (MQ=21)

                                                          cATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGtt                                                                                    <  2:2628765/148‑1 (MQ=21)

                                                            tatTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTct                                                                                  >  2:3550309/1‑148 (MQ=21)

                                                            tatTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTct                                                                                  >  2:1788706/1‑148 (MQ=21)

                                                            tatTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTct                                                                                  >  2:1149963/1‑148 (MQ=21)

                                                             atTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTctg                                                                                 >  1:1887792/1‑148 (MQ=21)

                                                             atTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTctg                                                                                 <  1:2811419/148‑1 (MQ=21)

                                                             atTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTctg                                                                                 >  2:444578/1‑148 (MQ=21)

                                                             atTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTctg                                                                                 >  2:1230395/1‑148 (MQ=21)

                                                                  ttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGAt                                                                            <  2:2951113/148‑1 (MQ=21)

                                                                      gATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTaa                                                                        <  1:2317221/148‑1 (MQ=21)

                                                                          tCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGc                                                                    >  1:1001531/1‑148 (MQ=21)

                                                                          tCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGc                                                                    <  2:49730/148‑1 (MQ=21)

                                                                                atgaACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGaa                                                              <  2:2084253/148‑1 (MQ=37)

                                                                                 tgaACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGaaa                                                             <  2:2375101/148‑1 (MQ=38)

                                                                                  gatatttttGATTTCAAGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAAt                                                            <  1:3278170/141‑1 (MQ=255)

                                                                                   aaCTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAAtt                                                           >  2:3439193/1‑148 (MQ=255)

                                                                                    aCTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTc                                                          >  1:117235/1‑148 (MQ=255)

                                                                                      ttATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGc                                                        <  2:1348665/148‑1 (MQ=255)

                                                                                        aTTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCca                                                      <  2:1596797/148‑1 (MQ=255)

                                                                                        aTTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCca                                                      <  1:386906/148‑1 (MQ=255)

                                                                                         ttGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCcac                                                     >  1:1033399/1‑148 (MQ=255)

                                                                                          tGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCcaca                                                    >  2:1369725/1‑148 (MQ=255)

                                                                                          tGATTACACGTTTGAATGGAAAGGGCTGAAGAAATTATGAGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGATCCAGAAATGACTGTCACATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCcaca                                                    >  1:2935424/1‑148 (MQ=14)

                                                                                             tttCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCg                                                 <  2:3272480/148‑1 (MQ=255)

                                                                                              ttCACGTTTGAATTGAATTGGATGATTAAATTATTCTTGGCATTCTCCTTTATGTCCATAATAGAAGAACAAAAGAAAGTGACAGTAATGACGGTCAGATTTTATATCAGTTATGCTGATTTAACCGCTGAGAAATTCGACAAAGCGa                                                <  2:2778458/148‑1 (MQ=255)

                                                                                              ttCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGa                                                <  2:3096533/148‑1 (MQ=255)

                                                                                               tCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGAt                                               <  1:3198076/148‑1 (MQ=255)

                                                                                                     ttGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGaaa                                         >  1:160421/1‑148 (MQ=255)

                                                                                                      tGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAAt                                        <  1:1145420/148‑1 (MQ=255)

                                                                                                        aaTGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCa                                      <  2:406353/148‑1 (MQ=255)

                                                                                                             aaaGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGc                                 >  2:1474266/1‑148 (MQ=255)

                                                                                                              aaGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCa                                >  2:5769/1‑148 (MQ=255)

                                                                                                              aaGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCa                                >  2:561779/1‑148 (MQ=255)

                                                                                                                   cTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTgg                           <  1:561829/148‑1 (MQ=255)

                                                                                                                   cTGAAGAAATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGAAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTgg                           <  1:183079/148‑1 (MQ=255)

                                                                                                                      aagaaATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGgaga                        <  2:245567/148‑1 (MQ=255)

                                                                                                                       agaaATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGaa                       <  1:631731/148‑1 (MQ=255)

                                                                                                                       agaaATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGaa                       >  2:813785/1‑148 (MQ=255)

                                                                                                                        gaaATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAAt                      >  2:2598702/1‑148 (MQ=255)

                                                                                                                        gaaATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAAt                      >  1:365830/1‑148 (MQ=255)

                                                                                                                        gaaATTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAAt                      >  2:1965156/1‑148 (MQ=255)

                                                                                                                         aaaTTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATa                     <  1:3298268/148‑1 (MQ=255)

                                                                                                                         aaaTTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATa                     <  2:1278161/148‑1 (MQ=255)

                                                                                                                           aTTATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg                   >  1:2534814/1‑148 (MQ=255)

                                                                                                                            ttATGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGc                  <  1:444578/148‑1 (MQ=255)

                                                                                                                              aTGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTg                >  1:309978/1‑148 (MQ=255)

                                                                                                                              aTGCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAAAAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTg                <  1:2204051/148‑1 (MQ=255)

                                                                                                                                gCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCa              <  2:105950/148‑1 (MQ=255)

                                                                                                                                gCGTGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCa              >  1:1236342/1‑148 (MQ=255)

                                                                                                                                   tGGCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTg           >  2:3368572/1‑148 (MQ=255)

                                                                                                                                     gCAGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc         >  1:405203/1‑148 (MQ=255)

                                                                                                                                       aGTCTCCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGt       <  1:1225517/148‑1 (MQ=255)

                                                                                                                                           tcCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTgg   >  1:2983244/1‑148 (MQ=255)

                                                                                                                                           tcCTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTgg   >  1:2567007/1‑148 (MQ=255)

                                                                                                                                            ccTTTAGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGa  <  1:1734464/148‑1 (MQ=255)

AGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGA  >  CP009273/3618391‑3618678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`