Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,639,239 G→T 100% P165P (CCG→CCT)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,639,2390GT95.8% 570.7 / 15.2 191P165P (CCG→CCT) rlmJ23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (86/96);  total (91/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 191 total aligned reads displayed.

GAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCG  >  CP009273/3639096‑3639383
                                                                                                                                               |                                                                                                                                                
gAACTGCACCCGAGCGATTACCCGTTTTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGt                                                                                                                                              <  1:2836556/148‑1 (MQ=255)
  aCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTat                                                                                                                                            >  2:2064329/1‑148 (MQ=255)
   cTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatg                                                                                                                                           >  1:3390483/1‑148 (MQ=255)
    tGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatga                                                                                                                                          >  2:288405/1‑148 (MQ=255)
      cACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaa                                                                                                                                        >  2:2335714/1‑148 (MQ=255)
      cACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaa                                                                                                                                        <  2:404085/148‑1 (MQ=255)
      cACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaa                                                                                                                                        >  2:540778/1‑148 (MQ=255)
      cACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaa                                                                                                                                        <  2:2197829/148‑1 (MQ=255)
          cGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaatgaa                                                                                                                                    >  1:423375/1‑148 (MQ=255)
           gAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTAtgaaatgaaa                                                                                                                                   >  2:224608/1‑148 (MQ=255)
            aGCGATTACCCGTTGGTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGaaaa                                                                                                                                  >  2:2899905/1‑148 (MQ=255)
                 ttACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGAc                                                                                                                             <  2:549782/148‑1 (MQ=255)
                  tACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGaaaa                                                                                                                                  >  2:1520499/1‑142 (MQ=255)
                   aCCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTa                                                                                                                           <  1:1460841/148‑1 (MQ=255)
                      cGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCa                                                                                                                        <  1:3415059/148‑1 (MQ=255)
                       gttgttGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCaa                                                                                                                       >  1:3269773/1‑148 (MQ=255)
                        ttgttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAg                                                                                                                      >  1:500835/1‑148 (MQ=255)
                            tgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggt                                                                                                                  <  1:1733951/148‑1 (MQ=255)
                            tgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggt                                                                                                                  <  1:223646/148‑1 (MQ=255)
                                ttCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTc                                                                                                              >  2:3532224/1‑148 (MQ=255)
                                ttCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTc                                                                                                              >  2:3532150/1‑148 (MQ=255)
                                 tCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCa                                                                                                             >  1:2571688/1‑148 (MQ=255)
                                 tCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCa                                                                                                             >  1:1405523/1‑148 (MQ=255)
                                      aTTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCTGCggg                                                                                                        >  1:1238752/1‑148 (MQ=255)
                                        ttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGAt                                                                                                      <  1:1186231/148‑1 (MQ=255)
                                           aGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGCTAGc                                                                                                   >  2:2956277/1‑148 (MQ=255)
                                           aGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGc                                                                                                   <  2:1754661/148‑1 (MQ=255)
                                           aGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGc                                                                                                   <  1:798226/148‑1 (MQ=255)
                                           aGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGc                                                                                                   <  1:1944073/148‑1 (MQ=255)
                                             aaaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAg                                                                                                 <  1:2653092/148‑1 (MQ=255)
                                             aaaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAg                                                                                                 <  1:1589821/148‑1 (MQ=255)
                                             aaaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAg                                                                                                 <  2:853753/148‑1 (MQ=255)
                                               aGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGaa                                                                                               <  2:1955840/148‑1 (MQ=255)
                                               aGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGaa                                                                                               >  1:448449/1‑148 (MQ=255)
                                               aGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGATTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGaa                                                                                               <  1:2175444/148‑1 (MQ=255)
                                                gATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAg                                                                                              >  2:1229890/1‑148 (MQ=255)
                                                 aTAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAgg                                                                                             <  2:2600810/148‑1 (MQ=255)
                                                  tAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGt                                                                                            <  2:2939513/148‑1 (MQ=255)
                                                    gCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTa                                                                                          <  1:708490/148‑1 (MQ=255)
                                                    gCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTa                                                                                          >  2:1564817/1‑148 (MQ=255)
                                                       gTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaa                                                                                       <  1:3379120/148‑1 (MQ=255)
                                                        tGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaaa                                                                                      >  1:1757639/1‑148 (MQ=255)
                                                        tGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaaa                                                                                      <  2:1129202/148‑1 (MQ=255)
                                                             gtgtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGttt                                                                                 <  1:2004099/148‑1 (MQ=255)
                                                             gtgtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTGTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGttt                                                                                 <  1:1211257/148‑1 (MQ=255)
                                                                tCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGc                                                                              >  2:472243/1‑148 (MQ=255)
                                                                 cGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGcc                                                                             >  1:2016784/1‑148 (MQ=255)
                                                                 cGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGcc                                                                             >  1:215343/1‑148 (MQ=255)
                                                                  gAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCa                                                                            <  2:1038217/148‑1 (MQ=255)
                                                                   ataaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCAc                                                                           <  1:2948010/146‑1 (MQ=255)
                                                                      aaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCCCTgg                                                                        >  2:2848121/1‑148 (MQ=255)
                                                                       aGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGt                                                                       <  2:207875/148‑1 (MQ=255)
                                                                       aGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGt                                                                       >  1:238370/1‑148 (MQ=255)
                                                                        gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTa                                                                      >  2:368280/1‑148 (MQ=255)
                                                                        gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATAGACCCACCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGAAGAAGGTTACAAACGTTTCGCCACTGGTa                                                                      >  1:2267055/1‑148 (MQ=255)
                                                                         ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                     <  2:1450972/148‑1 (MQ=255)
                                                                         ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                     <  2:3137557/148‑1 (MQ=255)
                                                                         ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                     <  2:137288/148‑1 (MQ=255)
                                                                         ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                     >  2:87446/1‑148 (MQ=255)
                                                                         ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                     >  2:87909/1‑148 (MQ=255)
                                                                         ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                     <  1:1394788/148‑1 (MQ=255)
                                                                          cGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAtt                                                                    <  2:1862703/148‑1 (MQ=255)
                                                                           gACGGTTTCCAGCAGCTTAAGGCCCAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                   <  1:572403/148‑1 (MQ=255)
                                                                           gACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                   <  1:280474/148‑1 (MQ=255)
                                                                                 ttCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCAc                                                             >  1:3103684/1‑148 (MQ=255)
                                                                                   ccAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtg                                                           <  2:780800/148‑1 (MQ=255)
                                                                                    cagcagCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgt                                                          <  1:817221/148‑1 (MQ=255)
                                                                                          cTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATc                                                    >  1:3475082/1‑148 (MQ=255)
                                                                                          cTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATc                                                    <  1:1564817/148‑1 (MQ=255)
                                                                                            tAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCg                                                  <  1:1170546/148‑1 (MQ=255)
                                                                                              aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggt                                                <  2:886404/148‑1 (MQ=255)
                                                                                               ggCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtg                                               <  1:244013/148‑1 (MQ=255)
                                                                                                gCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                              >  2:3490111/1‑148 (MQ=255)
                                                                                                  cAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGgtggtg                                            >  2:2615233/1‑148 (MQ=255)
                                                                                                    aaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgct                                          >  1:1002899/1‑148 (MQ=255)
                                                                                                     aCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                         >  2:85323/1‑148 (MQ=255)
                                                                                                     aCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                         >  1:1592482/1‑148 (MQ=255)
                                                                                                     aCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                         >  2:1889409/1‑148 (MQ=255)
                                                                                                         ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTc                                     <  1:288405/148‑1 (MQ=255)
                                                                                                           gccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAg                                   <  2:2458404/148‑1 (MQ=255)
                                                                                                              ggTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCaa                                <  1:2899905/148‑1 (MQ=255)
                                                                                                               gTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCaaa                               <  1:1410472/148‑1 (MQ=255)
                                                                                                                tttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                              <  2:3195625/148‑1 (MQ=255)
                                                                                                                tttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                              <  2:3103684/148‑1 (MQ=255)
                                                                                                                   cccGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTaa                           >  2:1231708/1‑148 (MQ=255)
                                                                                                                     cgccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAAGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgc                         >  1:3434039/1‑148 (MQ=255)
                                                                                                                       ccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgcgc                       >  1:2754569/1‑148 (MQ=255)
                                                                                                                        cgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGACAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                      <  2:3350838/148‑1 (MQ=255)
                                                                                                                        cgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                      <  1:2895692/148‑1 (MQ=255)
                                                                                                                         gTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCAt                     >  2:2648561/1‑148 (MQ=255)
                                                                                                                           ggTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGa                   >  2:1860543/1‑148 (MQ=255)
                                                                                                                              ttAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATcc                <  1:1527094/148‑1 (MQ=255)
                                                                                                                               tAATCCTTATCGACACTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa               <  2:489280/148‑1 (MQ=255)
                                                                                                                                 aTCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACg             >  1:3265953/1‑148 (MQ=255)
                                                                                                                                 aTCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACg             >  1:1014554/1‑148 (MQ=255)
                                                                                                                                 aTCATTATCGACACTCCGTATTAAATGAAAACTGACTTTCAAGCTGTGGTCAGGGGGATAGCAGAAGGTTACAAACGTTTCGACACTGGTATTTTCTCACTGTTGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACg             <  2:3254931/148‑1 (MQ=255)
                                                                                                                                  tCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGa            >  1:2022025/1‑148 (MQ=255)
                                                                                                                                            cccTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCg  >  1:1618603/1‑148 (MQ=255)
                                                                                                                                            cccTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCg  <  1:2064329/148‑1 (MQ=255)
                                                                                                                                            cccTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCg  >  1:3222479/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCG  >  CP009273/3639096‑3639383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑