Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,767,000 G→T 100% D454Y (GAC→TAC)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,0000GT97.6% 641.9 / 6.3 208D454Y (GAC→TAC) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (104/99);  total (105/103)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

GGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTT  >  CP009273/3766856‑3767145
                                                                                                                                                |                                                                                                                                                 
ggTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACa                                                                                                                                                >  2:2681741/1‑148 (MQ=255)
 gTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAg                                                                                                                                               >  1:1970544/1‑148 (MQ=255)
            aTTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTaca                                                                                                                                    >  1:2733131/1‑148 (MQ=255)
              ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACAcc                                                                                                                                  >  2:1034076/1‑148 (MQ=255)
              ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACAcc                                                                                                                                  >  2:596928/1‑148 (MQ=255)
              ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACAcc                                                                                                                                  >  1:685298/1‑148 (MQ=255)
                 tGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGc                                                                                                                               >  1:2802260/1‑148 (MQ=255)
                 tGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGc                                                                                                                               >  1:2238683/1‑148 (MQ=255)
                   ttACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCGGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCt                                                                                                                             >  2:1268692/1‑148 (MQ=255)
                   ttACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCt                                                                                                                             >  2:117410/1‑148 (MQ=255)
                      cTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGAc                                                                                                                          >  2:252736/1‑148 (MQ=255)
                            gcgcGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACg                                                                                                                    >  1:3167530/1‑148 (MQ=255)
                            gcgcGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGACTGACCGAACg                                                                                                                    >  2:2999008/1‑148 (MQ=255)
                             cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGt                                                                                                                   <  1:1693464/148‑1 (MQ=255)
                                gATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTg                                                                                                                >  2:1865123/1‑148 (MQ=255)
                                 aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTgg                                                                                                               >  1:2103401/1‑148 (MQ=255)
                                   caacaaCCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGtt                                                                                                             >  2:730513/1‑148 (MQ=255)
                                         ccTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGcc                                                                                                       >  1:2958561/1‑148 (MQ=255)
                                         ccTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGcc                                                                                                       <  2:1753171/148‑1 (MQ=255)
                                         ccTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGcc                                                                                                       >  1:1180925/1‑148 (MQ=255)
                                         ccTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGcc                                                                                                       <  2:1968200/148‑1 (MQ=255)
                                         ccTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAACCTGACCGAACGTCTGGTTGCTGcc                                                                                                       >  1:268428/1‑148 (MQ=255)
                                            gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa                                                                                                    <  2:1572969/148‑1 (MQ=255)
                                            gccgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa                                                                                                    >  1:325254/1‑148 (MQ=255)
                                             ccgctAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAAc                                                                                                   <  2:1408442/148‑1 (MQ=255)
                                              cgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACg                                                                                                  <  1:1334013/148‑1 (MQ=255)
                                               gccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGc                                                                                                 <  1:588393/148‑1 (MQ=255)
                                                ccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGcc                                                                                                >  2:1684421/1‑148 (MQ=255)
                                                 cAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCca                                                                                               <  2:3349184/148‑1 (MQ=255)
                                                   gATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCcaca                                                                                             >  2:3523201/1‑148 (MQ=255)
                                                    aTGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCacac                                                                                            >  2:41618/1‑148 (MQ=255)
                                                          aCCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCaaa                                                                                      <  2:2441704/148‑1 (MQ=255)
                                                          aCCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCaaa                                                                                      <  2:2008435/148‑1 (MQ=255)
                                                           ccTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAAc                                                                                     <  1:2169887/148‑1 (MQ=255)
                                                            cTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACg                                                                                    <  1:1724693/148‑1 (MQ=255)
                                                             tCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCGCAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGa                                                                                   <  2:2978318/148‑1 (MQ=255)
                                                              cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGaa                                                                                  >  1:1046606/1‑148 (MQ=255)
                                                               gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAag                                                                                 <  1:1960380/148‑1 (MQ=255)
                                                               gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAag                                                                                 <  2:3424120/148‑1 (MQ=255)
                                                                tcatcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                                                                                <  1:2974162/148‑1 (MQ=255)
                                                                 catcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAagag                                                                               <  2:1362794/148‑1 (MQ=255)
                                                                 catcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAagag                                                                               >  2:1208803/1‑148 (MQ=255)
                                                                 catcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAagag                                                                               >  2:1208094/1‑148 (MQ=255)
                                                                   tcactcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaa                                                                             <  1:373066/148‑1 (MQ=255)
                                                                   tcactcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaa                                                                             >  1:1608716/1‑148 (MQ=255)
                                                                   tcactcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaa                                                                             <  2:337882/148‑1 (MQ=255)
                                                                   tcactcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaa                                                                             <  1:25894/148‑1 (MQ=255)
                                                                        cacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaa                                                                        >  2:555417/1‑148 (MQ=255)
                                                                           cGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAg                                                                     >  2:2586132/1‑148 (MQ=255)
                                                                           cGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAg                                                                     <  1:2369288/148‑1 (MQ=255)
                                                                             tgacctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGAc                                                                   >  1:782021/1‑148 (MQ=255)
                                                                             tgacctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGAc                                                                   <  2:2215330/148‑1 (MQ=255)
                                                                              gacctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACa                                                                  >  2:1393792/1‑148 (MQ=255)
                                                                               acctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAg                                                                 >  1:623074/1‑148 (MQ=255)
                                                                                cctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc                                                                >  2:2545663/1‑148 (MQ=255)
                                                                                cctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc                                                                >  1:1160952/1‑148 (MQ=255)
                                                                                cctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc                                                                >  2:785955/1‑148 (MQ=255)
                                                                                cctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc                                                                >  1:2646805/1‑148 (MQ=255)
                                                                                 ctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGcc                                                               <  1:486057/148‑1 (MQ=255)
                                                                                    accGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGa                                                            <  2:1929547/148‑1 (MQ=255)
                                                                                     ccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGaa                                                           <  1:2914290/148‑1 (MQ=255)
                                                                                     ccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGaa                                                           <  1:501996/148‑1 (MQ=255)
                                                                                      cGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGaaa                                                          <  1:2220868/148‑1 (MQ=255)
                                                                                       gAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAg                                                         >  2:1522543/1‑148 (MQ=255)
                                                                                        aaggcgcTTGGCGCCATGTTCAGCAGGCACAGCATTTTTCTCTGAACAACTTCCTCTACAGCGGCCTGTACACCAGCATGACAGAACGTCTGGTTGCTGCCCTTCGCAAGTCGGCAAAAGTAGAGTAAGTAAAAGACAGCCTGAAAGa                                                        <  2:491723/145‑1 (MQ=255)
                                                                                        aaCGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGa                                                        >  1:292430/1‑148 (MQ=255)
                                                                                            cgcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGc                                                    <  1:664262/148‑1 (MQ=255)
                                                                                            cgcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGc                                                    <  1:2043229/148‑1 (MQ=255)
                                                                                             gcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCt                                                   <  1:1442171/148‑1 (MQ=255)
                                                                                                aTGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTg                                                <  1:1093082/148‑1 (MQ=255)
                                                                                                 tGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGa                                               <  2:2213596/148‑1 (MQ=255)
                                                                                                   cgcCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACg                                             <  2:3070280/148‑1 (MQ=255)
                                                                                                     ccAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGAt                                           <  2:3244835/148‑1 (MQ=255)
                                                                                                      cAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGAtt                                          <  1:85990/148‑1 (MQ=255)
                                                                                                        ggTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCACACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGAGTcc                                        >  2:1971984/1‑148 (MQ=255)
                                                                                                        ggTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTcc                                        >  1:3504286/1‑148 (MQ=255)
                                                                                                             cGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGc                                   <  1:274765/148‑1 (MQ=255)
                                                                                                              gCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCt                                  >  2:2925586/1‑148 (MQ=255)
                                                                                                               cAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGTGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTa                                 <  2:247388/148‑1 (MQ=255)
                                                                                                                aGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTaa                                <  2:729270/148‑1 (MQ=255)
                                                                                                                 ggCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAAc                               >  2:2185839/1‑148 (MQ=255)
                                                                                                                 ggCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAAc                               >  2:546903/1‑148 (MQ=255)
                                                                                                                  gCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAAcc                              >  1:1396732/1‑148 (MQ=255)
                                                                                                                    acaGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTg                            <  1:3544603/148‑1 (MQ=255)
                                                                                                                     caGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGt                           <  2:1822879/148‑1 (MQ=255)
                                                                                                                     caGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGt                           >  1:2898837/1‑148 (MQ=255)
                                                                                                                       gCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTc                         >  2:2656916/1‑148 (MQ=255)
                                                                                                                        cATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCa                        <  2:2105162/148‑1 (MQ=255)
                                                                                                                        cATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCa                        >  1:1765288/1‑148 (MQ=255)
                                                                                                                         atatTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCaa                       <  2:1019855/148‑1 (MQ=255)
                                                                                                                         atatTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCaa                       <  2:264180/148‑1 (MQ=255)
                                                                                                                          tatTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAg                      >  1:2163758/1‑148 (MQ=255)
                                                                                                                           atTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGc                     <  2:3179803/148‑1 (MQ=255)
                                                                                                                                       aaCTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGgaga         >  1:1374305/1‑148 (MQ=255)
                                                                                                                                        aCTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGaa        <  1:2784835/148‑1 (MQ=255)
                                                                                                                                         cTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGTGCGGAGAAc       <  1:2992444/148‑1 (MQ=255)
                                                                                                                                         cTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAAc       >  1:1906454/1‑148 (MQ=255)
                                                                                                                                           tCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACAt     <  1:467045/148‑1 (MQ=255)
                                                                                                                                            ccTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATc    <  1:1268692/148‑1 (MQ=255)
                                                                                                                                              tCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATcgt  <  1:906092/148‑3 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTT  >  CP009273/3766856‑3767145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑