Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,339 A→C 8.8% Q12P (CAA→CCA) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3390AC 8.8% 375.1 / 4.5 198Q12P (CAA→CCA) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (90/85);  new base C (16/7);  total (106/92)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAG  >  CP009273/3822192‑3822481
                                                                                                                                                   |                                                                                                                                               
tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCa                                                                                                                                                 >  1:821554/1‑148 (MQ=255)
   aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc‑c                                                                                                                                             >  1:901506/1‑148 (MQ=255)
     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑Cgg                                                                                                                                           <  1:284834/148‑1 (MQ=255)
      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGt                                                                                                                                          >  2:3025878/1‑148 (MQ=255)
         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTgc                                                                                                                                       >  2:1446628/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTgcg                                                                                                                                      >  2:1237966/1‑148 (MQ=255)
           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGGCAC‑CGGGTgcgc                                                                                                                                     >  1:2405190/1‑148 (MQ=255)
           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTgcgc                                                                                                                                     >  2:1727532/1‑148 (MQ=255)
           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTgcgc                                                                                                                                     <  1:840695/148‑1 (MQ=255)
             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAg                                                                                                                                   <  1:2098233/148‑1 (MQ=255)
              tttATCTCAGTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGa                                                                                                                                  >  2:1621278/1‑148 (MQ=255)
               ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGAc                                                                                                                                 >  2:1151748/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTTGCGCCGACTCa                                                                                                                              >  1:2247620/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCa                                                                                                                              >  1:188999/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCa                                                                                                                              >  1:1138155/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTCCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCa                                                                                                                              >  1:2095304/1‑148 (MQ=255)
                   ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCaa                                                                                                                             >  2:2242089/1‑148 (MQ=255)
                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAAA‑CCCTTGAGCAGACTaaaa                                                                                                                            >  1:328912/1‑148 (MQ=255)
                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCCTTGCGCAGACTCaaa                                                                                                                            >  1:1259311/1‑148 (MQ=255)
                     cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCaaaa                                                                                                                           >  2:580156/1‑148 (MQ=255)
                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAAcg                                                                                                                         <  2:2336543/148‑2 (MQ=255)
                            atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                    <  2:3048601/148‑1 (MQ=255)
                             tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                   >  2:2570970/1‑148 (MQ=255)
                              atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGTTGCGCAGACTCAAAAAAGCGAAca                                                                                                                  >  2:1552819/1‑147 (MQ=255)
                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                <  2:817966/148‑1 (MQ=255)
                                  aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCCAACAGAtt                                                                                                              >  2:1219392/1‑148 (MQ=255)
                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CCGTTGCGCAGACACAAAAAATCGAACAGAtttt                                                                                                            >  1:565449/1‑147 (MQ=255)
                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTCATTTa                                                                                                            >  2:2272603/1‑148 (MQ=255)
                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGTTGCGCAGACTCAAAACACCGAACTGATTTa                                                                                                            >  1:3093094/1‑148 (MQ=255)
                                     tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                           >  2:3498287/1‑148 (MQ=255)
                                               gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CCGGTGCGCCGACACAAAAAACAGCACAGATTTAAACTCTTGAAg                                                                                                 >  1:706541/1‑148 (MQ=255)
                                               gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAA‑CCGTTTCGCCCACAAAAAAAAGCGAAAATATTTTACGTCTTGAAg                                                                                                 >  1:291080/1‑148 (MQ=255)
                                                cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGa                                                                                                >  2:2657118/1‑148 (MQ=255)
                                                cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGACTTGaata                                                                                                >  2:1253357/1‑146 (MQ=255)
                                                 aaccGTTTGCTTTTCTCGTTCGGTCAGCTAAACGGCCCGTTTTGCTACCAGGGGATTTTTTTCGATGTGTGTTTCCACCCTCGAGTCATAAAATGCGCACC‑GGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAt                                                                                               <  1:3208478/145‑1 (MQ=255)
                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                              >  2:547007/1‑148 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                            >  2:1196009/1‑148 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                            <  2:2133274/148‑1 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                            <  2:173705/148‑1 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCTAGTCAGAAAATGCGAAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                            <  2:42812/148‑1 (MQ=255)
                                                     gTTTGCTTTTCTCTTTCGGTCAGAAAAACTGCCCGCTTTGCTACCAGGGGTTTGTTTTCGATGTCTGTTACCACCCTCGAGACACAAAATGCGCCCC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                           <  1:2079342/148‑1 (MQ=255)
                                                      tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CCGGTGCGCCGACTCAAAAAATCCAAAAGATTTAACGTCTTGAAGATCGTcc                                                                                          >  1:117886/1‑148 (MQ=255)
                                                      tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                          >  2:3233405/1‑148 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTccg                                                                                         >  2:641729/1‑148 (MQ=255)
                                                         gCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CCGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgcc                                                                                       >  1:1452791/1‑148 (MQ=255)
                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTTGCGCAGACACAAAAAACCGAACAGATTTAACGTCTTGAATATCGTACGCCGCtt                                                                                   >  2:537530/1‑148 (MQ=255)
                                                             ttCTCTTTCGGCAACATAACATGCCCGCTTTGCAACCTGGGTTTTTTTTTCGTTGTGTGTTCCCACCCTCGTGCAAGAAAATGCCCACC‑GGTTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                   <  1:557600/148‑1 (MQ=255)
                                                                  tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                              >  2:1284226/1‑148 (MQ=255)
                                                                  tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                              >  2:2678904/1‑148 (MQ=255)
                                                                   ttCGGTCAGATAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaa                                                                             <  2:874397/148‑1 (MQ=255)
                                                                       gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGTTGCGCAGACTCAAAACAGCGAACAGATTTAACGTCTTGTATATCGTGCGCCGCGTCCTCAAAacc                                                                         >  1:1329685/1‑148 (MQ=255)
                                                                         cAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTg                                                                       <  2:1281634/148‑1 (MQ=255)
                                                                         cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCGTCCTCAAAcccag                                                                       >  1:2946149/1‑146 (MQ=255)
                                                                           gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CCGGTGCGCAGAAACAAAAAATCGAACTGAATTAGCGGCGATCAGAACGTCCGCCGCGGACACAAACCCGGtt                                                                     >  1:3409140/1‑148 (MQ=255)
                                                                           gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCATCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                     <  2:3427965/148‑1 (MQ=255)
                                                                             tAATATGCCCGTTTTGATACCTGGTGTTTGTTTTCGTTGTCTGTTACCACCCTCGATACATAAATGGCGCACG‑CGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                   <  1:1383524/148‑1 (MQ=255)
                                                                             tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                   <  2:2657431/148‑1 (MQ=255)
                                                                                aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCGGGTGTgccg                                                                >  2:2140014/1‑148 (MQ=255)
                                                                                aaTGCCCGCTTTGCGACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTTGCGCAGACTCAAAACACCGAACTGATTTAACGTCTTGTAGATCGTCCGCCGCGTCCTCAAACCCAGTTTggcg                                                                >  1:2161044/1‑148 (MQ=255)
                                                                                  tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                              >  2:3203767/1‑148 (MQ=255)
                                                                                       gCTTTGCTACCCCGGGATTGTTGTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATCCGCAAC‑CGGTTGCGCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                         <  2:1828630/148‑1 (MQ=255)
                                                                                         tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                       >  2:2054024/1‑148 (MQ=255)
                                                                                            gCTACCAGGTGTTTGTTTTCGTTGTCTTTTTCCCCCCTCGAGAAAGAAATTGCGCAAC‑GGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                    <  1:203675/148‑1 (MQ=255)
                                                                                            gCTACCAGGGTATTGTTTTCGATGTCTTTTCCCACCCTCGAGTCATAAAAGGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                    <  1:1199903/148‑1 (MQ=255)
                                                                                            gCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                    <  2:240952/148‑1 (MQ=255)
                                                                                            gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                    <  2:2095304/148‑1 (MQ=255)
                                                                                              tcccACGGTTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGGC‑CTTTATGGGGAACGGGGTTGCGCAGACTCAACACAGCGAACTGATGTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCGGTCAGCATctg                                                  <  2:165868/146‑1 (MQ=255)
                                                                                              tACCACGGTTTTGTTTTCGTTGTCTGTTCCCCCCCCCGAGCCACAAAAGGCGCACC‑CGGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctg                                                  <  1:352885/148‑1 (MQ=255)
                                                                                               aCCACGGGATGTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                 <  2:3415694/148‑1 (MQ=255)
                                                                                                   cGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAAC‑CGGTTGCGCAGACTCAAAAAACAGAACTGATTTAACCTCTTGAAAATCGTCCGCCGCGTACTCAAACCCGGTTTTGCGCCAGTCAGCCTCTTCTGGc                                             >  1:750308/1‑148 (MQ=255)
                                                                                                        ttgttttgttTGTCTTTTCCCACCCTCCACACCCCAAATGCGCCCC‑CGGGGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                        <  1:641729/148‑1 (MQ=255)
                                                                                                        ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                        <  2:719970/148‑1 (MQ=255)
                                                                                                        ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                        <  2:605703/148‑1 (MQ=255)
                                                                                                         tGTTTTGGATGTCTGTTACCCCCCTCTCGACAGAAAATGCCCACC‑CGGTGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                       <  1:333218/148‑1 (MQ=255)
                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                     <  2:2950858/148‑1 (MQ=255)
                                                                                                             ttCGATGTGTGTGACCACCCTCGACTCACAAAAGGCGCCCC‑CGGGGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                   <  1:3004296/148‑1 (MQ=255)
                                                                                                              tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                  <  2:3528738/148‑1 (MQ=255)
                                                                                                                gATGTCTGTTTCCACCCTCGAGCCAGAAAATGCGCCCC‑GGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                <  1:2647065/148‑1 (MQ=255)
                                                                                                                 aTGTCTTTCACCACCCTCGACTCACAAAATGCGCACC‑CGGGTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                               <  1:617885/148‑1 (MQ=255)
                                                                                                                  tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                              <  2:951547/148‑1 (MQ=255)
                                                                                                                    tCTGTTTCCACCCTCGAGTCAGAAACTGCGCACC‑CGGGGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                            <  1:3148839/148‑1 (MQ=255)
                                                                                                                     cTGTTTCCACCCTAGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCACTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                           >  2:2705889/1‑148 (MQ=255)
                                                                                                                      tttttCCACCCTCTATTCATAAAATGCGAAAC‑GTTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTAGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                          <  2:3284626/146‑1 (MQ=255)
                                                                                                                      tGTTTCCACCCTCGAGTCATAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                          <  2:1515485/148‑1 (MQ=255)
                                                                                                                                 tCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt               >  2:3263500/1‑148 (MQ=255)
                                                                                                                                 tCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt               >  2:177410/1‑148 (MQ=255)
                                                                                                                                 tCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt               >  2:1971442/1‑148 (MQ=255)
                                                                                                                                 tCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGGCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt               >  2:1493083/1‑148 (MQ=255)
                                                                                                                                  cGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa              <  2:1793286/148‑1 (MQ=255)
                                                                                                                                     gacataaacTGCGCCCC‑CGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTa           <  1:1933202/143‑1 (MQ=255)
                                                                                                                                        aGAAACTGCGCACC‑CGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc        <  1:2430906/148‑1 (MQ=255)
                                                                                                                                         gAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt       >  2:3372313/1‑148 (MQ=255)
                                                                                                                                         gAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCGTCCTCAAAACCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATAACGCCACCGCTATTAATCt       >  1:1157935/1‑148 (MQ=255)
                                                                                                                                          aaatTGCGCACC‑GGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg      <  1:49332/148‑1 (MQ=255)
                                                                                                                                          aaaaTGCGCAAC‑CGGTTGCGCAGACTCAAAACACCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCAACTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATATg      >  2:702199/1‑148 (MQ=255)
                                                                                                                                          aaaaGGCGCACC‑GGGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg      <  1:48938/148‑1 (MQ=255)
                                                                                                                                             aTGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa   <  2:3040579/148‑1 (MQ=255)
                                                                                                                                             aTGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa   >  2:2010294/1‑148 (MQ=255)
                                                                                                                                              tGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg  >  2:2963883/1‑148 (MQ=255)
                                                                                                                                              tGCGAAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACAGTATTGAATATCGTCCGCCGCTAACTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg  >  2:3563437/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAG  >  CP009273/3822192‑3822481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑