Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,826,247 A→T 44.6% Y551N (TAC→AAC)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,826,2470AT44.6% 95.7 / 306.0 217Y551N (TAC→AAC) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (57/63);  new base T (51/46);  total (108/109)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

CCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAA  >  CP009273/3826100‑3826392
                                                                                                                                                   |                                                                                                                                                 
   gATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGc                                                                                                                                                >  1:2676858/1‑148 (MQ=255)
   gATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGc                                                                                                                                                <  2:2934989/148‑1 (MQ=255)
    aTCGTCCGGGACCTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGcc                                                                                                                                               >  2:1748469/1‑148 (MQ=255)
    aTCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGcc                                                                                                                                               >  2:464106/1‑148 (MQ=255)
      cGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCa                                                                                                                                             <  2:2133340/148‑1 (MQ=255)
      cGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCa                                                                                                                                             >  2:1458480/1‑148 (MQ=255)
        tCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACg                                                                                                                                           >  1:621783/1‑148 (MQ=255)
               aCTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACg                                                                                                                                    <  2:139351/148‑1 (MQ=255)
               aCTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGGTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACg                                                                                                                                    <  2:1527952/148‑1 (MQ=255)
                 tCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGAt                                                                                                                                  <  2:2667762/148‑1 (MQ=255)
                 tCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGAt                                                                                                                                  >  1:2565497/1‑148 (MQ=255)
                 tCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGAt                                                                                                                                  >  2:1332636/1‑148 (MQ=255)
                    atcatcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAg                                                                                                                               >  2:1348614/1‑148 (MQ=255)
                     tcatcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGa                                                                                                                              <  2:1171413/148‑1 (MQ=255)
                      catcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGAt                                                                                                                             <  1:2424695/148‑1 (MQ=255)
                        tcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATACGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGAttt                                                                                                                           >  2:1989337/1‑148 (MQ=255)
                         catGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTg                                                                                                                          <  1:2518905/148‑1 (MQ=255)
                         catGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGAATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTg                                                                                                                          <  1:2787734/148‑1 (MQ=255)
                         catGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGAATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTg                                                                                                                          <  1:1972216/148‑1 (MQ=255)
                          atGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGc                                                                                                                         <  1:2966895/148‑1 (MQ=255)
                            ggCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTa                                                                                                                       >  1:176888/1‑148 (MQ=255)
                             gCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTAc                                                                                                                      >  2:255538/1‑148 (MQ=255)
                               ccGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCg                                                                                                                    <  2:3055507/148‑1 (MQ=255)
                                    tcatcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTaa                                                                                                               >  1:3333530/1‑148 (MQ=255)
                                     catcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa                                                                                                              >  2:1194394/1‑148 (MQ=255)
                                     catcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa                                                                                                              >  1:1476359/1‑148 (MQ=255)
                                     catcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa                                                                                                              >  1:3247068/1‑148 (MQ=255)
                                        cGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACgg                                                                                                           >  2:1824019/1‑148 (MQ=255)
                                         ggCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGc                                                                                                          <  2:1267445/148‑1 (MQ=255)
                                          gCGTACCCCGCGCGGTCGCACGCGCAGCTACACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTAGCTACCGTGTAAACGGCt                                                                                                         <  2:2475157/148‑1 (MQ=255)
                                           cGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTACACGGCTa                                                                                                        >  2:2651091/1‑148 (MQ=255)
                                           cGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTa                                                                                                        >  2:864254/1‑148 (MQ=255)
                                               ccccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGc                                                                                                    <  2:222248/148‑1 (MQ=255)
                                               ccccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACAACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGc                                                                                                    <  1:2712462/148‑1 (MQ=255)
                                                cccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCt                                                                                                   <  1:3556224/148‑1 (MQ=255)
                                                   gcgcgTTCGCACGCGCAGCTTCACGATACTGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGga                                                                                                <  1:490427/148‑1 (MQ=255)
                                                   gcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGga                                                                                                >  2:3313645/1‑148 (MQ=255)
                                                   gcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGga                                                                                                >  2:2504702/1‑148 (MQ=255)
                                                      cggtCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGagag                                                                                             <  1:2005354/145‑1 (MQ=255)
                                                             aCGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaacca                                                                                      >  2:2805134/1‑148 (MQ=255)
                                                              cgcgCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaaccaa                                                                                     <  1:1217785/148‑1 (MQ=255)
                                                              cgcgCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaaccaa                                                                                     <  2:3503689/148‑1 (MQ=255)
                                                              cgcgCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaaccaa                                                                                     <  1:1440695/148‑1 (MQ=255)
                                                                   aGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAAcgcg                                                                                <  2:321856/148‑1 (MQ=255)
                                                                      ttCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCAc                                                                             <  1:2382841/148‑1 (MQ=255)
                                                                      ttCACGATACAGAAACTGCATCATGCGGCATTTCTGTTGCGTGAAGAAGCGCACCAAATCACAGGACTCATCATCGTAGGCCCACGGCACAAGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCAc                                                                             <  1:592577/148‑1 (MQ=255)
                                                                       tCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGATGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCAcc                                                                            >  1:290662/1‑148 (MQ=255)
                                                                           gATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCg                                                                        <  2:2167747/148‑1 (MQ=255)
                                                                            aTACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGt                                                                       >  2:1377538/1‑148 (MQ=255)
                                                                              aCAGATACGGCATCCTGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGCCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGttt                                                                     <  2:89239/148‑1 (MQ=255)
                                                                               cAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTg                                                                    >  1:1109673/1‑148 (MQ=255)
                                                                                  aTACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTaa                                                                 <  1:2154721/148‑1 (MQ=255)
                                                                                  aTACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACAGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTaa                                                                 >  2:2336656/1‑148 (MQ=255)
                                                                                        catcatGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTgc                                                           >  2:1430436/1‑148 (MQ=255)
                                                                                           catGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGcc                                                        <  1:2364041/148‑1 (MQ=255)
                                                                                            atGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCg                                                       <  1:2840835/148‑1 (MQ=255)
                                                                                              gCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGa                                                     <  1:1667677/148‑1 (MQ=255)
                                                                                                  cATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCgg                                                 <  1:685273/148‑1 (MQ=255)
                                                                                                   aTTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGt                                                <  2:139872/148‑1 (MQ=255)
                                                                                                   aTTTCAGGTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGt                                                <  2:3541922/148‑1 (MQ=255)
                                                                                                    tttCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTa                                               <  1:1628717/148‑1 (MQ=255)
                                                                                                    tttCAGGTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTa                                               <  2:430097/148‑1 (MQ=255)
                                                                                                     ttCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTAt                                              >  1:210532/1‑148 (MQ=255)
                                                                                                       cAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTAttt                                            <  2:2178083/148‑1 (MQ=255)
                                                                                                         gTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTc                                          >  1:879252/1‑148 (MQ=255)
                                                                                                          ttGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCa                                         >  1:2330671/1‑148 (MQ=255)
                                                                                                             cGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAg                                      <  1:79839/148‑1 (MQ=255)
                                                                                                                gaagaaGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAgccg                                   <  2:129498/148‑1 (MQ=255)
                                                                                                                    aagCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGa                               <  1:478789/148‑1 (MQ=255)
                                                                                                                     agCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGat                              >  2:1361906/1‑148 (MQ=255)
                                                                                                                     agCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGat                              <  1:1419481/148‑1 (MQ=255)
                                                                                                                      gcgcACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGata                             >  2:62628/1‑148 (MQ=255)
                                                                                                                         caccacATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCg                          >  2:3329556/1‑148 (MQ=255)
                                                                                                                         caccacATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCg                          <  2:375366/148‑1 (MQ=255)
                                                                                                                           ccacATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTg                        >  2:3095567/1‑148 (MQ=255)
                                                                                                                           ccacATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTg                        >  1:1164824/1‑148 (MQ=255)
                                                                                                                           ccacATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTg                        >  2:1434772/1‑148 (MQ=255)
                                                                                                                           ccacATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTg                        >  1:1060994/1‑148 (MQ=255)
                                                                                                                             acaTCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGc                      <  1:1713541/148‑1 (MQ=255)
                                                                                                                              caTCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCt                     <  2:1591329/148‑1 (MQ=255)
                                                                                                                               aTCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTc                    >  2:975688/1‑148 (MQ=255)
                                                                                                                               aTCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTc                    <  1:1081093/148‑1 (MQ=255)
                                                                                                                               aTCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTc                    >  2:2755800/1‑148 (MQ=255)
                                                                                                                                  acaGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAg                 >  2:502765/1‑148 (MQ=255)
                                                                                                                                    aGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGaa               <  2:1311658/148‑1 (MQ=255)
                                                                                                                                     ggACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAg              <  1:1729170/148‑1 (MQ=255)
                                                                                                                                     ggACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAg              <  1:3190524/148‑1 (MQ=255)
                                                                                                                                      gACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGc             >  1:2976358/1‑148 (MQ=255)
                                                                                                                                      gACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGc             >  2:1009664/1‑148 (MQ=255)
                                                                                                                                       aCTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGcc            >  1:2825762/1‑148 (MQ=255)
                                                                                                                                        cTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCa           <  1:464106/148‑1 (MQ=255)
                                                                                                                                         tcatcaTCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCaa          >  2:1571849/1‑148 (MQ=255)
                                                                                                                                          catcatCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCaaa         >  1:1263415/1‑148 (MQ=255)
                                                                                                                                          catcatCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCaaa         >  1:3445546/1‑148 (MQ=255)
                                                                                                                                             catcGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAAcc      >  2:700061/1‑148 (MQ=255)
                                                                                                                                             catcGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAAcc      <  1:2558446/148‑1 (MQ=255)
                                                                                                                                             catcGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAAcc      <  1:2554486/148‑1 (MQ=255)
                                                                                                                                               tcGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTg    >  1:2864328/1‑148 (MQ=255)
                                                                                                                                               tcGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTg    >  1:18717/1‑148 (MQ=255)
                                                                                                                                                 gTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGaa  <  2:2685634/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAA  >  CP009273/3826100‑3826392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑