Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,830,819 T→G 100% F169L (TTT→TTG)  setC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,830,8190TG96.5% 896.7 / 7.4 226F169L (TTT→TTG) setCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (3/5);  new base G (96/122);  total (99/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 226 total aligned reads displayed.

CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCG  >  CP009273/3830674‑3830962
                                                                                                                                                 |                                                                                                                                               
cAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAg                                                                                                                                               <  1:2701403/148‑1 (MQ=255)
 aaaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGt                                                                                                                                              <  1:2001456/148‑1 (MQ=255)
 aaaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGt                                                                                                                                              >  1:2704348/1‑148 (MQ=255)
 aaaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGt                                                                                                                                              >  1:531601/1‑148 (MQ=255)
  aaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGtt                                                                                                                                             >  1:1643268/1‑148 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                            <  1:1054102/148‑1 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                            <  2:1767933/148‑1 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                            <  2:184186/148‑1 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGACAATGGGATTGAGttt                                                                                                                                            >  2:2553626/1‑148 (MQ=255)
       gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTaaa                                                                                                                                        <  1:3012578/148‑1 (MQ=255)
       gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTaaa                                                                                                                                        <  1:1769014/148‑1 (MQ=255)
        cAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAg                                                                                                                                       <  2:2672235/148‑1 (MQ=255)
         aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGt                                                                                                                                      >  1:2866826/1‑148 (MQ=255)
           aTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGa                                                                                                                                    <  1:3460253/148‑1 (MQ=255)
             gTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATg                                                                                                                                  <  1:1491570/148‑1 (MQ=255)
               tcgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGAAATGGGATTGAGTTTTAAAGTGATGTa                                                                                                                                <  2:2903055/148‑1 (MQ=255)
                cgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTTCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAt                                                                                                                               <  2:2839511/148‑1 (MQ=255)
                    ctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCa                                                                                                                           <  1:2571225/148‑1 (MQ=255)
                     tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCAc                                                                                                                          <  1:781848/148‑1 (MQ=255)
                     tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCAc                                                                                                                          <  2:1611925/148‑1 (MQ=255)
                        cccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGc                                                                                                                       >  1:3297991/1‑148 (MQ=255)
                            tGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGcc                                                                                                                   >  1:806064/1‑148 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                  >  1:634543/1‑148 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                  >  1:402839/1‑148 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                  <  2:3028000/148‑1 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                  >  1:2425328/1‑148 (MQ=255)
                               acacGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATc                                                                                                                >  1:1097449/1‑148 (MQ=255)
                                   gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAt                                                                                                            <  1:589549/148‑1 (MQ=255)
                                   gCCGAAAGAACAGGCCGTGATACGGTCATGTTCAGTAAATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCGGCCATCGCAt                                                                                                            <  2:272250/148‑1 (MQ=255)
                                       aCAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgt                                                                                                        <  1:496815/148‑1 (MQ=255)
                                        cAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgtt                                                                                                       <  1:462142/148‑1 (MQ=255)
                                        cAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgtt                                                                                                       <  1:2255679/148‑1 (MQ=255)
                                         aGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgttg                                                                                                      <  1:1525005/148‑1 (MQ=255)
                                          gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTtgttgt                                                                                                     >  2:3480832/1‑148 (MQ=255)
                                          gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTtgttgt                                                                                                     >  1:2916128/1‑148 (MQ=255)
                                          gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTtgttgt                                                                                                     <  1:396703/148‑1 (MQ=255)
                                           aaCAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTgttgtt                                                                                                    >  2:529115/1‑148 (MQ=255)
                                           aaCAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTgttgtt                                                                                                    <  2:3290990/148‑1 (MQ=255)
                                             cAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAt                                                                                                                               >  2:3451046/1‑119 (MQ=255)
                                                 ccGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGa                                                                                              <  1:2592377/148‑1 (MQ=255)
                                                 ccGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGa                                                                                              <  2:1470428/148‑1 (MQ=255)
                                                 ccGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGa                                                                                              <  1:2403669/148‑1 (MQ=255)
                                                  cGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGAc                                                                                             <  1:2763728/148‑1 (MQ=255)
                                                    tGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTg                                                                                           <  1:1389371/148‑1 (MQ=255)
                                                     gagaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGa                                                                                          <  2:1986196/148‑1 (MQ=255)
                                                      agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                         <  2:1879592/148‑1 (MQ=255)
                                                      agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                         <  1:2113629/148‑1 (MQ=255)
                                                       gaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATa                                                                                        >  1:1329256/1‑148 (MQ=255)
                                                        aCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAg                                                                                       >  2:1113362/1‑148 (MQ=255)
                                                         cGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGt                                                                                      <  2:589495/148‑1 (MQ=255)
                                                          ggTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTc                                                                                     <  1:283224/148‑1 (MQ=255)
                                                             cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTgg                                                                                  >  1:1001402/1‑148 (MQ=255)
                                                                gTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttg                                                                               <  1:1813980/148‑1 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                              >  2:851631/1‑148 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                              <  2:1536011/148‑1 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                              <  1:2077571/148‑1 (MQ=255)
                                                                  tCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgtt                                                                             >  2:754400/1‑148 (MQ=255)
                                                                     gTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGttttt                                                                          >  1:1159170/1‑148 (MQ=255)
                                                                      tACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTg                                                                         <  1:1710601/148‑1 (MQ=255)
                                                                      tACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTg                                                                         >  1:71084/1‑148 (MQ=255)
                                                                      tACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTg                                                                         >  1:248316/1‑148 (MQ=255)
                                                                       aCATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGc                                                                        >  2:2202890/1‑148 (MQ=255)
                                                                          tttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCAt                                                                     >  1:2113380/1‑148 (MQ=255)
                                                                          tttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCAt                                                                     <  2:1367766/148‑1 (MQ=255)
                                                                           ttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATc                                                                    >  1:84407/1‑148 (MQ=255)
                                                                            tttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCa                                                                   <  2:448957/148‑1 (MQ=255)
                                                                               aCGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATa                                                                >  2:3513548/1‑148 (MQ=255)
                                                                                cGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATAc                                                               <  1:575505/148‑1 (MQ=255)
                                                                                cGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCAACGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATAc                                                               <  1:2366943/148‑1 (MQ=255)
                                                                                  tGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaa                                                             <  1:3359177/148‑1 (MQ=255)
                                                                                   gCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaaa                                                            <  1:3047766/148‑1 (MQ=255)
                                                                                     tCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGa                                                          <  2:1643268/148‑1 (MQ=255)
                                                                                      cAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGaa                                                         <  2:1255650/148‑1 (MQ=255)
                                                                                         aTCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAata                                                      >  2:3293095/1‑148 (MQ=255)
                                                                                         aTCTCGCTTGCCTGGGTTATCGGACCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAata                                                      <  1:3455662/148‑1 (MQ=255)
                                                                                          tctcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAatat                                                     <  2:3397285/148‑1 (MQ=255)
                                                                                             cGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTcc                                                  >  1:2204739/1‑148 (MQ=255)
                                                                                              gCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCt                                                 <  1:2233633/148‑1 (MQ=255)
                                                                                                ttGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGt                                               <  2:2893789/148‑1 (MQ=255)
                                                                                                  gCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCg                                             <  1:1972080/148‑1 (MQ=255)
                                                                                                    cTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGtt                                           >  2:3412709/1‑148 (MQ=255)
                                                                                                     tGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTa                                          <  1:1624933/148‑1 (MQ=255)
                                                                                                      gggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTAc                                         >  1:544931/1‑148 (MQ=255)
                                                                                                       ggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTAcc                                        >  2:815910/1‑148 (MQ=255)
                                                                                                            tCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAcc                                   <  1:3171108/148‑1 (MQ=255)
                                                                                                                     cgctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAt                          >  1:1965835/1‑148 (MQ=255)
                                                                                                                     cgctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAt                          <  1:1437206/148‑1 (MQ=255)
                                                                                                                     cgctcgctCATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAt                          >  1:1966412/1‑148 (MQ=255)
                                                                                                                      gctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAtt                         >  2:410968/1‑148 (MQ=255)
                                                                                                                        tcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAtttt                       <  1:1512671/148‑1 (MQ=255)
                                                                                                                          gctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAc                     <  2:1958973/148‑1 (MQ=255)
                                                                                                                           ctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAcc                    >  2:144666/1‑148 (MQ=255)
                                                                                                                             tATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCt                  <  2:2465348/148‑1 (MQ=255)
                                                                                                                              aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTTTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                 >  1:2301382/1‑148 (MQ=255)
                                                                                                                               tGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcc                >  2:1795949/1‑148 (MQ=255)
                                                                                                                                       cAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAg        >  1:1230383/1‑148 (MQ=255)
                                                                                                                                         aTGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGa      >  2:3457708/1‑148 (MQ=255)
                                                                                                                                         aTGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGa      >  2:1503965/1‑148 (MQ=255)
                                                                                                                                          tGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGaa     >  1:3564803/1‑148 (MQ=255)
                                                                                                                                            ggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTACTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGc   >  1:2985450/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCG  >  CP009273/3830674‑3830962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑