Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,865,863 T→A 100% T166S (ACC→TCC)  dgoD ← galactonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,865,8630TA96.3% 680.5 / 11.5 213T166S (ACC→TCC) dgoDgalactonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (3/5);  new base A (107/98);  total (110/103)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

CCAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCAACT  >  CP009273/3865719‑3866007
                                                                                                                                                |                                                                                                                                                
ccAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGtt                                                                                                                                               <  1:1135649/148‑1 (MQ=255)
  aGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTaa                                                                                                                                             <  2:1005850/148‑1 (MQ=255)
   gCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAc                                                                                                                                            >  2:2947778/1‑148 (MQ=255)
    cACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAcc                                                                                                                                           >  2:2606030/1‑148 (MQ=255)
      ccGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgc                                                                                                                                         <  1:1907082/148‑1 (MQ=255)
      ccGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgc                                                                                                                                         >  1:1663368/1‑148 (MQ=255)
      ccGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGACACGGAGTTAAccgc                                                                                                                                         >  1:1663425/1‑148 (MQ=255)
       cGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgcc                                                                                                                                        >  1:2753032/1‑148 (MQ=255)
       cGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTAAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgcc                                                                                                                                        >  1:2753617/1‑148 (MQ=255)
          ctcctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCa                                                                                                                                     >  1:3396224/1‑148 (MQ=255)
            cctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATc                                                                                                                                   <  2:3357497/148‑1 (MQ=255)
              tcAATAAACCGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTa                                                                                                                                 <  1:354451/148‑1 (MQ=255)
               cAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTAc                                                                                                                                <  2:3489746/148‑1 (MQ=255)
               cAATAAACAGCGGGCGATACGGCTCCAGTTATTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCAACGGAGTTAAACGCCGCATCTAc                                                                                                                                >  1:1215836/1‑148 (MQ=255)
                 aTAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcg                                                                                                                              >  1:3067000/1‑148 (MQ=255)
                 aTAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcg                                                                                                                              >  1:899098/1‑148 (MQ=255)
                  tAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcgc                                                                                                                             >  1:478197/1‑148 (MQ=255)
                   aaaCAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACcgcg                                                                                                                            >  2:2885161/1‑148 (MQ=255)
                        gCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGa                                                                                                                       <  1:2959638/148‑1 (MQ=255)
                         cGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTGCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAg                                                                                                                      <  1:1541665/148‑1 (MQ=255)
                          gggCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGt                                                                                                                     >  2:3127936/1‑148 (MQ=255)
                             cGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCACTCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTAt                                                                                                                  <  2:2908325/148‑1 (MQ=255)
                             cGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTAt                                                                                                                  <  1:2010292/148‑1 (MQ=255)
                               aTACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTCCCGCGCGGGAGTTATCa                                                                                                                >  2:1609386/1‑148 (MQ=255)
                               aTACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCa                                                                                                                <  2:3420184/148‑1 (MQ=255)
                                tACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCaa                                                                                                               <  2:2677381/148‑1 (MQ=255)
                                  cGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAtt                                                                                                             >  1:2424863/1‑148 (MQ=255)
                                   ggCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTa                                                                                                            >  2:669210/1‑148 (MQ=255)
                                   ggCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTa                                                                                                            >  2:2458593/1‑148 (MQ=255)
                                     cTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGc                                                                                                          <  2:2491724/148‑1 (MQ=255)
                                       ccAGTTATTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGccc                                                                                                        <  2:1002075/148‑1 (MQ=255)
                                            tCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGtt                                                                                                   >  2:3529188/1‑148 (MQ=255)
                                             cTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttc                                                                                                  >  2:1341475/1‑148 (MQ=255)
                                             cTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttc                                                                                                  <  1:596518/148‑1 (MQ=255)
                                             cTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttc                                                                                                  <  1:1330362/148‑1 (MQ=255)
                                               ttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAGACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttctt                                                                                                <  2:729007/148‑1 (MQ=255)
                                               ttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttctt                                                                                                >  2:2017325/1‑148 (MQ=255)
                                                  aTCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGAATGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcac                                                                                             >  2:1231132/1‑148 (MQ=255)
                                                   tCAGCTCTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAAATCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcaca                                                                                            <  2:2181924/148‑1 (MQ=255)
                                                    cAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACaa                                                                                           <  2:2167218/148‑1 (MQ=255)
                                                    cAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACaa                                                                                           <  2:1108779/148‑1 (MQ=255)
                                                    cAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACaa                                                                                           >  2:3372770/1‑148 (MQ=255)
                                                     aGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAAc                                                                                          <  1:2581488/148‑1 (MQ=255)
                                                      gCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAAcc                                                                                         >  2:1084301/1‑148 (MQ=255)
                                                        aCTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGt                                                                                       <  1:1560159/148‑1 (MQ=255)
                                                        aCTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGt                                                                                       <  2:1406822/148‑1 (MQ=255)
                                                         cTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGtt                                                                                      >  2:3432802/1‑148 (MQ=255)
                                                          tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                     <  1:1941989/148‑1 (MQ=255)
                                                          tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                     <  1:1341475/148‑1 (MQ=255)
                                                             cGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGt                                                                                  <  1:3229017/148‑1 (MQ=255)
                                                                  tCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGaa                                                                             >  2:1659661/1‑148 (MQ=255)
                                                                  tCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTACACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGaa                                                                             <  2:282669/148‑1 (MQ=255)
                                                                   cGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAg                                                                            >  2:757223/1‑148 (MQ=255)
                                                                   cGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAg                                                                            >  2:600874/1‑148 (MQ=255)
                                                                     gcgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGt                                                                          <  1:1628517/148‑1 (MQ=255)
                                                                     gcgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGt                                                                          <  1:1628560/148‑1 (MQ=255)
                                                                      cgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGNGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTa                                                                         <  1:1806701/148‑1 (MQ=255)
                                                                      cgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTa                                                                         <  2:1850683/148‑1 (MQ=255)
                                                                       gcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTAt                                                                        <  1:720447/148‑1 (MQ=255)
                                                                          cTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAATCTTCACGAATTTGTGCCACGGAGTTAACCGCCTCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGa                                                                     <  2:3097191/148‑1 (MQ=255)
                                                                          cTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGa                                                                     <  1:776925/148‑1 (MQ=255)
                                                                           tGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCTAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGaa                                                                    <  2:1921312/148‑1 (MQ=255)
                                                                            gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                   >  2:2466262/1‑148 (MQ=255)
                                                                            gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                   <  2:837401/148‑1 (MQ=255)
                                                                               gcgACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCg                                                                <  1:3476223/148‑1 (MQ=255)
                                                                                cgACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGa                                                               <  2:786264/148‑1 (MQ=255)
                                                                                  aCCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAtt                                                             >  1:2091492/1‑148 (MQ=255)
                                                                                  aCCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAtt                                                             >  2:393401/1‑148 (MQ=255)
                                                                                        gAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGt                                                       <  1:1577666/148‑1 (MQ=255)
                                                                                        gAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGt                                                       >  1:962217/1‑148 (MQ=255)
                                                                                           aTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGc                                                    >  2:1715029/1‑148 (MQ=255)
                                                                                                gACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGtttt                                               >  1:2515930/1‑148 (MQ=255)
                                                                                                  ccAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTaa                                             <  2:108624/148‑1 (MQ=255)
                                                                                                   cAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAAt                                            <  1:2139447/148‑1 (MQ=255)
                                                                                                   cAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAAt                                            >  2:2482080/1‑148 (MQ=255)
                                                                                                    aaaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAGTTCGCGTAGCGTTTTAATg                                           >  2:220490/1‑148 (MQ=255)
                                                                                                      aCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGcc                                         >  1:1727164/1‑148 (MQ=255)
                                                                                                        tCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGt                                       >  2:1749849/1‑148 (MQ=255)
                                                                                                        tCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGt                                       <  1:3127936/148‑1 (MQ=255)
                                                                                                          aaTCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCg                                     <  2:2754671/148‑1 (MQ=255)
                                                                                                          aaTCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCg                                     <  2:2753115/148‑1 (MQ=255)
                                                                                                            tCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGAt                                   >  1:474156/1‑148 (MQ=255)
                                                                                                              tGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATaa                                 <  2:3401926/148‑1 (MQ=255)
                                                                                                                aTTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACa                               <  2:135988/148‑1 (MQ=255)
                                                                                                                 ttGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACAt                              <  2:2656141/148‑1 (MQ=255)
                                                                                                                 ttGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACAt                              <  1:2075006/148‑1 (MQ=255)
                                                                                                                  tGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATc                             >  1:150399/1‑148 (MQ=255)
                                                                                                                    ccAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATccg                           >  1:2853631/1‑148 (MQ=255)
                                                                                                                       aaaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATccgccg                        >  1:1270244/1‑148 (MQ=255)
                                                                                                                          gCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGAc                     >  2:1650459/1‑148 (MQ=255)
                                                                                                                            ttCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGa                   <  1:1786997/148‑1 (MQ=255)
                                                                                                                             tCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGAt                  >  2:2142410/1‑148 (MQ=255)
                                                                                                                                cGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgc               >  2:3023692/1‑148 (MQ=255)
                                                                                                                                  aaTTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgccg             >  1:1883148/1‑148 (MQ=255)
                                                                                                                                     ttGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGcca          <  2:1220451/148‑1 (MQ=255)
                                                                                                                                      tgtgCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGccaa         >  2:1599662/1‑148 (MQ=255)
                                                                                                                                           cACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGccaacccaa    <  1:2348387/148‑1 (MQ=255)
                                                                                                                                           cACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGccaacccaa    <  2:1547375/148‑1 (MQ=255)
                                                                                                                                            aCGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGccaacccaac   >  1:3164025/1‑148 (MQ=255)
                                                                                                                                            aCGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGccaacccaac   >  2:2554853/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCAACT  >  CP009273/3865719‑3866007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑