Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,871,008 G→T 13.8% intergenic (‑183/+57) yidB ← / ← gyrB DUF937 family protein/DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,871,0080GT13.8% 656.8 / 59.0 253intergenic (‑183/+57)yidB/gyrBDUF937 family protein/DNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base G (134/82);  new base T (17/20);  total (151/102)
Fisher's exact test for biased strand distribution p-value = 7.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

Only 100 of 258 total aligned reads displayed.

AAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCGTGGTGAACAAC  >  CP009273/3870861‑3871155
                                                                                                                                                   |                                                                                                                                                   
 caGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGa                                                                                                                                                    <  1:1689108/147‑1 (MQ=255)
 aaGTATAGATTTGCGGGATGATAAGTGCGGATTGCAGAAATACAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGa                                                                                                                                                    <  2:1808719/148‑1 (MQ=255)
   gTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAtt                                                                                                                                                  >  1:3377076/1‑148 (MQ=255)
    tataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATAttt                                                                                                                                                 <  2:973823/148‑1 (MQ=255)
     ataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATAATGAAAATTCAATACATTGCAAGAtttt                                                                                                                                                >  1:2747836/1‑148 (MQ=255)
       aGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCg                                                                                                                                              <  2:3334804/148‑1 (MQ=255)
       aGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCg                                                                                                                                              <  2:2852580/148‑1 (MQ=255)
                ggATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGa                                                                                                                                     >  2:2554501/1‑148 (MQ=255)
                ggATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGa                                                                                                                                     >  2:3111867/1‑148 (MQ=255)
                 gatgatAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGAt                                                                                                                                    >  1:264122/1‑148 (MQ=255)
                  atgatAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATa                                                                                                                                   <  1:1531569/148‑1 (MQ=255)
                  atgatAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATa                                                                                                                                   <  2:3279645/148‑1 (MQ=255)
                     atAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGc                                                                                                                                <  1:871697/148‑1 (MQ=255)
                       aaTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGt                                                                                                                              <  2:3068751/148‑1 (MQ=255)
                       aaTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGt                                                                                                                              <  1:3456075/148‑1 (MQ=255)
                        aTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTa                                                                                                                             >  2:2646898/1‑148 (MQ=255)
                         ttGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAg                                                                                                                            <  2:2279589/148‑1 (MQ=255)
                         ttGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAg                                                                                                                            >  2:1147106/1‑148 (MQ=255)
                          tGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAgc                                                                                                                           >  1:2222403/1‑148 (MQ=255)
                            cGGAGTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAgcgc                                                                                                                         <  1:1825301/148‑1 (MQ=255)
                             ggATTGCAGAAAAAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCa                                                                                                                        <  1:1000407/148‑1 (MQ=255)
                              gATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCAt                                                                                                                       <  2:2207006/148‑1 (MQ=255)
                              gATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCAt                                                                                                                       >  1:3473726/1‑148 (MQ=255)
                                 tGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                    >  2:1649739/1‑148 (MQ=255)
                                      aaaTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACg                                                                                                               >  2:1125180/1‑148 (MQ=255)
                                      aaaTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGGAGGCCTGATAAGCGTAGCGCAGAAGGCACg                                                                                                               >  1:152051/1‑148 (MQ=255)
                                        aTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCt                                                                                                             >  2:853104/1‑148 (MQ=255)
                                        aTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCt                                                                                                             >  2:854267/1‑148 (MQ=255)
                                            aacgggCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCa                                                                                                         <  2:816252/145‑1 (MQ=255)
                                             aaGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCAt                                                                                                        >  1:2851571/1‑148 (MQ=255)
                                              aGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATg                                                                                                       >  1:235677/1‑148 (MQ=255)
                                              aGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATg                                                                                                       >  2:2784849/1‑148 (MQ=255)
                                               gggCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATgg                                                                                                      >  2:1778929/1‑148 (MQ=255)
                                                ggCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGt                                                                                                     >  2:1050195/1‑148 (MQ=255)
                                                    gagaTGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATAAAATGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAgc                                                                                                 >  1:2414727/1‑148 (MQ=255)
                                                     agaTGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAAAACAATGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAgcg                                                                                                >  1:779538/1‑148 (MQ=255)
                                                        tgatCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCa                                                                                             <  2:103410/148‑1 (MQ=255)
                                                         gatCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCAt                                                                                            >  2:2504245/1‑148 (MQ=255)
                                                           tctcCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTTCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTa                                                                                          >  1:428209/1‑148 (MQ=255)
                                                            ctcCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAAAATGAAAATTCAATACATTTCAAGATTTTCGTAGGCCTGATAATCGTAGCGCATCAGGCACGCGCGCATGGTTAGCGCCATTaa                                                                                         >  1:2416225/1‑148 (MQ=25)
                                                              ccGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAat                                                                                       <  2:1496486/148‑1 (MQ=255)
                                                                   cTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGa                                                                                  >  2:2587821/1‑148 (MQ=255)
                                                                   cTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGa                                                                                  >  1:2902070/1‑148 (MQ=255)
                                                                    ttttCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCAACAACATCATGAAAATTCAATCCATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGat                                                                                 <  2:2736118/148‑1 (MQ=255)
                                                                      ttCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACAATGAAAGATATTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGatat                                                                               >  2:2387652/1‑148 (MQ=255)
                                                                       tCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATTAAAATTCAATACCTTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATAtt                                                                              <  2:44036/148‑1 (MQ=255)
                                                                       tCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATAtt                                                                              <  2:2682820/148‑1 (MQ=255)
                                                                          tataGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTcgc                                                                           >  2:881781/1‑148 (MQ=255)
                                                                             aGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTcgccgc                                                                        >  1:2967031/1‑148 (MQ=255)
                                                                              gCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCt                                                                       <  2:2581189/148‑1 (MQ=255)
                                                                                 tcttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTc                                                                    >  1:2712739/1‑148 (MQ=255)
                                                                                  cttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCa                                                                   >  2:1525072/1‑148 (MQ=255)
                                                                                  cttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCa                                                                   >  1:2684818/1‑148 (MQ=255)
                                                                                  cttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCa                                                                   >  1:568053/1‑148 (MQ=255)
                                                                                    tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATTAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAgg                                                                 <  2:664270/148‑1 (MQ=255)
                                                                                     gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAggg                                                                <  2:713073/148‑1 (MQ=255)
                                                                                       gggATGCGGCTTGACCGCCTAACCCGCACAACAAAATCATTAAAATTCAATACATTCAAATTTTTTCTTTGGCCTGTTACGGGTAGCGCAACCGCCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCg                                                              <  1:116989/147‑1 (MQ=255)
                                                                                        ggATGCGGGGTGAACGCATTAACCGGCCTAAAAAATCATTAAAATTCATAACATTGCATTTTTTTGGTAGGCCTTATACGGGTCGCCAATCTGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATAGTCGCCGCTTTCAGGGCGt                                                             <  1:3469515/148‑1 (MQ=255)
                                                                                         gATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGtt                                                            >  1:1868480/1‑148 (MQ=255)
                                                                                           tGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTtct                                                          >  1:89215/1‑148 (MQ=255)
                                                                                            gcggcgTGAACGCCTTATCCGGCCTACAAAATCTTGAAAATTCAATACAGTGCAAGATTTTGGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTtctc                                                         <  2:611758/148‑1 (MQ=255)
                                                                                            gcggcgTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTtctc                                                         >  2:2839467/1‑148 (MQ=255)
                                                                                              ggcgTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCtt                                                       <  2:1996483/148‑1 (MQ=255)
                                                                                                 gTGAACGCCTTATCCGGCCAAAAAAATCATTAAAATTCAATACTTTGAAATATTTTCGTAGGCCTTATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCaa                                                    <  1:1948460/148‑1 (MQ=255)
                                                                                                   gAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATa                                                  >  1:3314728/1‑148 (MQ=255)
                                                                                                   gAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATa                                                  >  2:2403139/1‑148 (MQ=255)
                                                                                                     aCGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATaaa                                                <  2:543849/148‑1 (MQ=255)
                                                                                                     aCGCCTTATCCGGCCAACAAAATCATGAAAATTCAATACTTTGCAAGTTTTTCGTAGGCCTTATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATaaa                                                <  1:3111867/148‑1 (MQ=255)
                                                                                                     aCGCCTTATCCGGCCAACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATaaa                                                <  2:2243792/148‑1 (MQ=255)
                                                                                                     aCGCCTTATCCGGCATAAAAAATCATTAAAATTAAATACATTGAAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATaaa                                                <  1:2634541/148‑1 (MQ=38)
                                                                                                       gCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACg                                              >  1:1306051/1‑148 (MQ=255)
                                                                                                           tATCCGGCCAACAAAATCATGAAAATTCAATACATTGAAAGTGTTTCGTTGGCCTGATAAGCGTAGCGCATCCGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACg                                          <  1:1400625/148‑1 (MQ=38)
                                                                                                             tCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGgc                                        >  2:1891801/1‑148 (MQ=255)
                                                                                                              ccGGCCTACAAAATAATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGgcg                                       >  2:3524316/1‑148 (MQ=255)
                                                                                                                 gCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCgg                                    >  1:2193579/1‑148 (MQ=255)
                                                                                                                 gCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCgg                                    >  2:1929363/1‑148 (MQ=255)
                                                                                                                 gCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCgg                                    <  2:2851571/148‑1 (MQ=255)
                                                                                                                     aCAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCAGGGTTAGCGCCATTAAATATCGATCTTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCa                                >  1:502331/1‑148 (MQ=255)
                                                                                                                        aaaTCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGAGATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACg                             >  2:2223112/1‑148 (MQ=255)
                                                                                                                           tCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCg                          <  2:944319/148‑1 (MQ=255)
                                                                                                                            cATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGt                         >  1:3195117/1‑148 (MQ=255)
                                                                                                                             aTGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTc                        >  2:1651515/1‑148 (MQ=255)
                                                                                                                             aTGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTc                        >  1:3525267/1‑148 (MQ=255)
                                                                                                                                  aaTTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGAGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCa                   >  2:2133019/1‑148 (MQ=255)
                                                                                                                                  aaTTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGAGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCa                   >  2:1370348/1‑148 (MQ=255)
                                                                                                                                   aTTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCAt                  >  1:99918/1‑148 (MQ=255)
                                                                                                                                    ttCAATCCATTGCAATTTTTTCGTTGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATc                 <  1:1029601/148‑1 (MQ=255)
                                                                                                                                    ttCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATc                 >  1:3561710/1‑148 (MQ=255)
                                                                                                                                     tCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCa                >  2:2963797/1‑148 (MQ=255)
                                                                                                                                     tCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCa                >  1:3507202/1‑148 (MQ=255)
                                                                                                                                     tCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAAAGCACGGCGCGGTTCAACGGCGTCGCCCATCa                >  2:2964001/1‑148 (MQ=255)
                                                                                                                                        aTACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCg             >  1:700745/1‑148 (MQ=255)
                                                                                                                                         tACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCgt            <  2:3132252/148‑1 (MQ=255)
                                                                                                                                           ccttGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATCAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCgtgg          <  2:1317335/146‑1 (MQ=255)
                                                                                                                                            aTTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCgtggt         <  2:3468393/148‑1 (MQ=255)
                                                                                                                                            aTTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCgtggt         >  2:2120855/1‑148 (MQ=255)
                                                                                                                                             ttGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCgtggtg        >  2:1248885/1‑148 (MQ=255)
                                                                                                                                                cAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCGTGGTGaac     >  1:1120123/1‑148 (MQ=255)
                                                                                                                                                   gATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCGTGGTGaacaac  >  1:652983/1‑148 (MQ=255)
                                                                                                                                                   gATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCGTGGTGaacaac  >  1:652770/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCGTGGTGAACAAC  >  CP009273/3870861‑3871155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑