Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,892,304 C→A 100% P150P (CCG→CCT)  yieK ← putative 6‑phosphogluconolactonase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,892,3040CA95.1% 543.3 / 18.0 184P150P (CCG→CCT) yieKputative 6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base C (2/7);  new base A (101/74);  total (103/81)
Fisher's exact test for biased strand distribution p-value = 4.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 184 total aligned reads displayed.

CTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAA  >  CP009273/3892159‑3892446
                                                                                                                                                 |                                                                                                                                              
cTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAgg                                                                                                                                              >  1:1772820/1‑148 (MQ=255)
  ttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGa                                                                                                                                            <  2:1137387/148‑1 (MQ=255)
  ttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGa                                                                                                                                            >  2:1835153/1‑148 (MQ=255)
   tACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGaa                                                                                                                                           <  2:2356500/148‑1 (MQ=255)
     cccGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACt                                                                                                                                         <  1:2026750/148‑1 (MQ=255)
      ccGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTc                                                                                                                                        >  1:2994072/1‑148 (MQ=255)
            cgcTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGt                                                                                                                                  <  1:2856593/148‑1 (MQ=255)
             gcTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGtt                                                                                                                                 <  2:2508495/148‑1 (MQ=255)
              cTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGttt                                                                                                                                >  1:703244/1‑148 (MQ=255)
               tGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTg                                                                                                                               <  2:1047010/148‑1 (MQ=255)
               tGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTg                                                                                                                               <  2:1729341/148‑1 (MQ=255)
                 aCAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCt                                                                                                                             >  1:2922611/1‑148 (MQ=255)
                  cAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTc                                                                                                                            >  1:627797/1‑148 (MQ=255)
                  cAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTc                                                                                                                            <  2:3340829/148‑1 (MQ=255)
                    aTTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCAt                                                                                                                          >  2:2764861/1‑148 (MQ=255)
                    aTTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCAt                                                                                                                          >  2:1898392/1‑148 (MQ=255)
                     ttATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATg                                                                                                                         <  2:1375105/148‑1 (MQ=255)
                      tATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATgg                                                                                                                        >  2:3268327/1‑148 (MQ=255)
                         cAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaaa                                                                                                                     >  2:3468567/1‑148 (MQ=255)
                          aGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAAt                                                                                                                    <  2:2655894/148‑1 (MQ=255)
                          aGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAAt                                                                                                                    <  2:2729523/148‑1 (MQ=255)
                            cAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATgg                                                                                                                  <  1:3152164/148‑1 (MQ=255)
                            cAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATgg                                                                                                                  <  2:1863627/148‑1 (MQ=255)
                            cAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATgg                                                                                                                  >  1:1108373/1‑148 (MQ=255)
                            cAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCACCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATgg                                                                                                                  >  2:940212/1‑148 (MQ=255)
                                  tttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTa                                                                                                            <  2:594886/148‑1 (MQ=255)
                                     cgccgcCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTc                                                                                                         <  1:497441/148‑1 (MQ=255)
                                       ccgccATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCgg                                                                                                       <  2:2176122/148‑1 (MQ=255)
                                           cATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCaaa                                                                                                   <  2:198739/148‑1 (MQ=255)
                                           cATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCaaa                                                                                                   <  2:655299/148‑1 (MQ=255)
                                             tgatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAAtt                                                                                                 >  1:2377121/1‑148 (MQ=255)
                                             tgatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAAtt                                                                                                 >  1:3229853/1‑148 (MQ=255)
                                              gatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTg                                                                                                >  1:105645/1‑148 (MQ=255)
                                                tgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGcc                                                                                              >  1:2416912/1‑148 (MQ=255)
                                                 gCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCca                                                                                             >  1:3350254/1‑148 (MQ=255)
                                                 gCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCca                                                                                             >  1:2166183/1‑148 (MQ=255)
                                                       cGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACaaaaa                                                                                       <  1:3080763/148‑1 (MQ=255)
                                                          ccccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGa                                                                                    >  2:242355/1‑148 (MQ=255)
                                                          ccccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGa                                                                                    <  1:1472343/148‑1 (MQ=255)
                                                            ccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGAcc                                                                                  <  2:444710/148‑1 (MQ=255)
                                                            ccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGAcc                                                                                  <  2:190459/148‑1 (MQ=255)
                                                               tCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATc                                                                               <  2:2994072/148‑1 (MQ=255)
                                                                cGTCACGTAGCTGTCCGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGACCTCCACGGTTTGCTCATGGACATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCt                                                                              >  2:3024379/1‑148 (MQ=255)
                                                                  tCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTTGTATTCGGCAAATTGCCACAAAAATGACCATCTGc                                                                            >  1:977844/1‑148 (MQ=255)
                                                                   cACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCa                                                                           >  1:1657884/1‑148 (MQ=255)
                                                                   cACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCa                                                                           >  1:680947/1‑148 (MQ=255)
                                                                       tAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTa                                                                       >  1:2424961/1‑148 (MQ=255)
                                                                          cTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATc                                                                    <  2:2989287/148‑1 (MQ=255)
                                                                          cTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATc                                                                    <  1:2616487/148‑1 (MQ=255)
                                                                          cTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATc                                                                    >  1:2902784/1‑148 (MQ=255)
                                                                          cTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATc                                                                    >  1:1927304/1‑148 (MQ=255)
                                                                           tGTCAGTCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAAtac                                                                   <  2:3153417/148‑3 (MQ=255)
                                                                            gTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATccc                                                                  <  2:1536911/148‑1 (MQ=255)
                                                                             tCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCa                                                                 >  1:2074908/1‑148 (MQ=255)
                                                                                ggCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGca                                                              >  1:1034448/1‑148 (MQ=255)
                                                                                 gCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGcac                                                             <  2:543614/148‑1 (MQ=255)
                                                                                 gCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGcac                                                             <  2:543544/148‑1 (MQ=255)
                                                                                   aCTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGcacca                                                           >  2:3190976/1‑148 (MQ=255)
                                                                                   aCTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGcacca                                                           >  2:3377142/1‑148 (MQ=255)
                                                                                    cTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGcaccac                                                          >  1:1225772/1‑148 (MQ=255)
                                                                                      aaCGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCAccacca                                                        >  1:321797/1‑148 (MQ=255)
                                                                                       aCGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCaa                                                       <  2:1184408/148‑1 (MQ=255)
                                                                                         gAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAAt                                                     <  2:2475705/148‑1 (MQ=255)
                                                                                          aaaaGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATc                                                    >  2:516153/1‑148 (MQ=255)
                                                                                             aGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAg                                                 >  2:1044420/1‑148 (MQ=255)
                                                                                               tCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTc                                               <  2:130327/148‑1 (MQ=255)
                                                                                               tCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTc                                               >  1:2543716/1‑148 (MQ=255)
                                                                                                    gccCAACTCGCCATGCGCCACAATATCACCCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCt                                          <  1:1360556/148‑1 (MQ=255)
                                                                                                     cccAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACTGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCtt                                         <  1:1350003/148‑1 (MQ=255)
                                                                                                       cAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCa                                       <  2:842803/148‑1 (MQ=255)
                                                                                                         aCTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCAcg                                     >  1:183815/1‑148 (MQ=255)
                                                                                                          cTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCAcgc                                    >  2:354752/1‑148 (MQ=255)
                                                                                                            cGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACgcgc                                  >  1:598095/1‑148 (MQ=255)
                                                                                                               cATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAg                               >  1:18680/1‑148 (MQ=255)
                                                                                                                aTGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGt                              >  1:1925477/1‑148 (MQ=255)
                                                                                                                 tGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGtt                             >  2:1659914/1‑148 (MQ=255)
                                                                                                                  gcgcCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGttt                            >  1:3445569/1‑148 (MQ=255)
                                                                                                                      cacaATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGa                        >  2:1004161/1‑148 (MQ=255)
                                                                                                                        caATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATc                      >  1:2427140/1‑148 (MQ=255)
                                                                                                                          atatCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCAt                    >  1:3241234/1‑148 (MQ=255)
                                                                                                                           tatCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATg                   <  1:2702242/148‑1 (MQ=255)
                                                                                                                            atCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGc                  >  1:982063/1‑148 (MQ=255)
                                                                                                                            atCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGc                  >  1:22532/1‑148 (MQ=255)
                                                                                                                             tCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCt                 >  2:109522/1‑148 (MQ=255)
                                                                                                                              cAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTc                >  2:3055177/1‑148 (MQ=255)
                                                                                                                              cAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTc                >  1:2412946/1‑148 (MQ=255)
                                                                                                                              cAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTc                >  2:241252/1‑148 (MQ=255)
                                                                                                                              cAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTc                >  2:2604399/1‑148 (MQ=255)
                                                                                                                               aaCCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTcg               <  2:1228783/148‑1 (MQ=255)
                                                                                                                                aCCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTcgc              >  1:784944/1‑148 (MQ=255)
                                                                                                                                 ccATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTcgcg             >  1:844255/1‑148 (MQ=255)
                                                                                                                                  cATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCgg            >  1:1136682/1‑148 (MQ=255)
                                                                                                                                     ctctcCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGGGGCAGTTTCTGATCATGCTCGCGGTAg         <  2:1962211/148‑1 (MQ=255)
                                                                                                                                     ctctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAg         <  1:2261194/148‑1 (MQ=255)
                                                                                                                                     ctctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAg         <  2:703244/148‑1 (MQ=255)
                                                                                                                                     ctctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAg         <  1:312995/148‑1 (MQ=255)
                                                                                                                                           cTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCa   >  1:282938/1‑148 (MQ=255)
                                                                                                                                            tGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCaa  <  2:2880864/148‑1 (MQ=255)
                                                                                                                                            tGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCaa  <  2:2074233/148‑1 (MQ=255)
                                                                                                                                            tGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCaa  >  1:233900/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAA  >  CP009273/3892159‑3892446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑