Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,901,415 C→A 100% P104P (CCG→CCT)  pstB ← phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,901,4150CA98.3% 540.8 / 0.8 172P104P (CCG→CCT) pstBphosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (90/79);  total (91/81)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

AGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG  >  CP009273/3901270‑3901556
                                                                                                                                                 |                                                                                                                                             
 gTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGa                                                                                                                                            >  2:2684252/1‑148 (MQ=255)
  tAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGaa                                                                                                                                           >  1:1190350/1‑148 (MQ=255)
  tAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGaa                                                                                                                                           >  2:3525809/1‑148 (MQ=255)
    aCCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAAc                                                                                                                                         <  2:3405236/148‑1 (MQ=255)
        ctctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCg                                                                                                                                     <  1:543473/148‑1 (MQ=255)
        ctctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCg                                                                                                                                     <  1:2212065/148‑1 (MQ=255)
        ctctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAAGGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCg                                                                                                                                     <  1:1837738/148‑1 (MQ=255)
          ctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTc                                                                                                                                   >  2:1835462/1‑148 (MQ=255)
           tGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCg                                                                                                                                  <  2:605397/148‑1 (MQ=255)
            ggTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCgg                                                                                                                                 <  2:3331432/148‑1 (MQ=255)
             gTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGt                                                                                                                                >  1:282713/1‑148 (MQ=255)
             gTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGt                                                                                                                                >  2:3265305/1‑148 (MQ=255)
             gTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGt                                                                                                                                >  2:1863784/1‑148 (MQ=255)
             gTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGt                                                                                                                                >  2:410497/1‑148 (MQ=255)
               gCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGttt                                                                                                                              >  2:3377261/1‑148 (MQ=255)
                cAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTc                                                                                                                             <  2:1633312/148‑1 (MQ=255)
                cAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTc                                                                                                                             <  2:607031/148‑1 (MQ=255)
                 aaTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCt                                                                                                                            >  1:2735492/1‑148 (MQ=255)
                  aTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTg                                                                                                                           >  1:2337022/1‑148 (MQ=255)
                    ttATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGa                                                                                                                         >  2:558839/1‑148 (MQ=255)
                    ttATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGa                                                                                                                         >  2:1940311/1‑148 (MQ=255)
                     tATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGaa                                                                                                                        <  1:3114139/148‑1 (MQ=255)
                     tATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGaa                                                                                                                        <  1:3566970/148‑1 (MQ=255)
                      aTCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCg                                                                                                                                  <  1:588639/137‑1 (MQ=255)
                      aTCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCg                                                                                                                                  >  2:588639/1‑137 (MQ=255)
                         tttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACAcc                                                                                                                    <  2:1707545/148‑1 (MQ=255)
                          ttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCa                                                                                                                   <  1:2272911/148‑1 (MQ=255)
                               ttCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGccc                                                                                                              >  1:2396834/1‑148 (MQ=255)
                                   ttCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACtt                                                                                                          >  2:552490/1‑148 (MQ=255)
                                    tCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACttt                                                                                                         >  1:3352872/1‑148 (MQ=255)
                                      cacaATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcg                                                                                                       >  2:3091003/1‑148 (MQ=255)
                                      cacaATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcg                                                                                                       >  1:3275854/1‑148 (MQ=255)
                                         aaTGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcac                                                                                                    >  2:138007/1‑148 (MQ=255)
                                         aaTGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcac                                                                                                    >  2:2278536/1‑148 (MQ=255)
                                          aTGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCCAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGGTTCTGGAACACCTTGCCCTCTTGcggccg                                                                                                   >  2:1947078/1‑144 (MQ=255)
                                           tGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacgc                                                                                                  >  1:393459/1‑148 (MQ=255)
                                            gCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacgca                                                                                                 >  2:2288669/1‑148 (MQ=255)
                                              ggCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACgcagc                                                                                               >  1:2582709/1‑148 (MQ=255)
                                               gCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACgcagca                                                                                              <  2:3360766/148‑1 (MQ=255)
                                               gCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACgcagca                                                                                              <  1:1455951/148‑1 (MQ=255)
                                                   tGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGc                                                                                          <  2:2371210/148‑1 (MQ=255)
                                                      tCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGat                                                                                       <  2:570856/148‑1 (MQ=255)
                                                      tCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGat                                                                                       <  1:2695113/148‑1 (MQ=255)
                                                       cAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGata                                                                                      <  2:413232/148‑1 (MQ=255)
                                                        aaTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGatat                                                                                     <  1:1074519/148‑1 (MQ=255)
                                                          tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATcc                                                                                   <  2:954453/148‑1 (MQ=255)
                                                             ccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTga                                                                                <  2:2092433/148‑1 (MQ=255)
                                                                cTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGagag                                                                             >  1:957297/1‑148 (MQ=255)
                                                                cTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGagag                                                                             >  1:219986/1‑148 (MQ=255)
                                                                 tGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGt                                                                            <  2:1589026/148‑1 (MQ=255)
                                                                  gCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGtt                                                                           >  1:184007/1‑148 (MQ=255)
                                                                   cACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGGAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTg                                                                          >  2:1390770/1‑148 (MQ=255)
                                                                     cgcgCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGt                                                                        <  2:1097953/148‑1 (MQ=255)
                                                                     cgcgCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGt                                                                        <  1:2360442/148‑1 (MQ=255)
                                                                     cgcgCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGt                                                                        <  1:569797/148‑1 (MQ=255)
                                                                       cgcTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCa                                                                      >  1:192337/1‑148 (MQ=255)
                                                                          tCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGa                                                                   <  1:1035087/148‑1 (MQ=255)
                                                                           cGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAt                                                                  >  1:1234333/1‑148 (MQ=255)
                                                                            gTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtg                                                                 <  1:2421350/148‑1 (MQ=255)
                                                                            gTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtg                                                                 <  2:2646667/148‑1 (MQ=255)
                                                                            gTCCATGTAGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtg                                                                 <  2:3551211/148‑1 (MQ=255)
                                                                            gTCCATGTAGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtg                                                                 <  2:1789085/148‑1 (MQ=255)
                                                                             tCCACGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgt                                                                <  2:1287251/148‑1 (MQ=255)
                                                                              ccATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                               >  1:958963/1‑148 (MQ=255)
                                                                              ccATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                               >  1:1465403/1‑148 (MQ=255)
                                                                              ccATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                               >  1:2313102/1‑148 (MQ=255)
                                                                              ccATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                               >  2:2989563/1‑148 (MQ=255)
                                                                                   tCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGc                                                          >  1:887271/1‑148 (MQ=255)
                                                                                      gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGTAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCAt                                                       <  1:435914/148‑1 (MQ=255)
                                                                                      gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCAt                                                       >  2:439754/1‑148 (MQ=255)
                                                                                      gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCAt                                                       >  2:3556633/1‑148 (MQ=255)
                                                                                      gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCAt                                                       >  2:2236407/1‑148 (MQ=255)
                                                                                        aCGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCa                                                     <  1:552490/148‑1 (MQ=255)
                                                                                           ggAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGc                                                  <  1:3144953/148‑1 (MQ=255)
                                                                                           ggAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGc                                                  <  2:1763758/148‑1 (MQ=255)
                                                                                            gagaGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                 >  1:1120322/1‑148 (MQ=255)
                                                                                            gagaGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                 >  1:612841/1‑148 (MQ=255)
                                                                                                 cTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                            >  2:2803568/1‑148 (MQ=255)
                                                                                                   tctcAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTc                                          >  1:3049763/1‑148 (MQ=255)
                                                                                                       aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCt                                      >  1:1983810/1‑148 (MQ=255)
                                                                                                          cAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTcc                                   >  2:2993250/1‑148 (MQ=255)
                                                                                                            gACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGc                                 >  1:2506632/1‑148 (MQ=255)
                                                                                                                  cGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctg                           <  2:393817/148‑1 (MQ=255)
                                                                                                                    ccAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgct                         <  1:3525809/148‑1 (MQ=255)
                                                                                                                     cAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                        <  1:3105928/148‑1 (MQ=255)
                                                                                                                     cAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                        >  1:758383/1‑148 (MQ=255)
                                                                                                                         aGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCggg                    <  2:3275854/148‑1 (MQ=255)
                                                                                                                         aGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCggg                    <  2:1190350/148‑1 (MQ=255)
                                                                                                                            gATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAc                 >  1:1632689/1‑148 (MQ=255)
                                                                                                                            gATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAc                 >  1:1503933/1‑148 (MQ=255)
                                                                                                                             aTGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                >  2:2305886/1‑148 (MQ=255)
                                                                                                                              tgttgtCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg               >  2:2674159/1‑148 (MQ=255)
                                                                                                                                ttgtCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCTGGTACAGtt             <  2:867073/148‑1 (MQ=255)
                                                                                                                                ttgtCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGtt             <  2:3418769/148‑1 (MQ=255)
                                                                                                                                ttgtCGTAGATGGAAATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGtt             >  2:1666391/1‑148 (MQ=255)
                                                                                                                                   tCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaa          >  2:2217496/1‑148 (MQ=255)
                                                                                                                                   tCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaa          <  1:2105747/148‑1 (MQ=255)
                                                                                                                                   tCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaa          >  2:2217386/1‑148 (MQ=255)
                                                                                                                                      tAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACa       >  1:2466662/1‑148 (MQ=255)
                                                                                                                                           ggACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttg  <  1:483656/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
AGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG  >  CP009273/3901270‑3901556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑