Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,659 C→A 100% R615S (CGC→AGC)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,6590CA95.1% 467.8 / 15.7 162R615S (CGC→AGC) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (90/64);  total (95/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 162 total aligned reads displayed.

GCGGCCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATC  >  CP009273/32516‑32802
                                                                                                                                               |                                                                                                                                               
gcggcCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCct                                                                                                                                             <  2:2678084/148‑1 (MQ=255)
 cggcCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCctc                                                                                                                                            <  2:580655/148‑1 (MQ=255)
   gcCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTa                                                                                                                                          <  2:2473408/148‑1 (MQ=255)
      cGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACtt                                                                                                                                       <  1:736137/148‑1 (MQ=255)
        aaCCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCg                                                                                                                                     >  2:3190294/1‑148 (MQ=255)
        aaCCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCg                                                                                                                                     <  2:2401766/148‑1 (MQ=255)
        aaCCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCg                                                                                                                                     <  2:545693/148‑1 (MQ=255)
        aaCCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCg                                                                                                                                     >  2:519981/1‑148 (MQ=255)
          ccGTATCGGTCAGGGTCTCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAg                                                                                                                                   >  1:2693838/1‑148 (MQ=255)
          ccGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAg                                                                                                                                   >  2:740619/1‑148 (MQ=255)
          ccGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAg                                                                                                                                   >  2:42207/1‑148 (MQ=255)
          ccGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGCAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAg                                                                                                                                   >  2:462024/1‑148 (MQ=255)
          ccGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGCAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAg                                                                                                                                   >  2:460092/1‑148 (MQ=255)
          ccGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGCAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAg                                                                                                                                   >  2:1633233/1‑148 (MQ=255)
          ccGTATCGGTCAGGGTATCGAATTCGACTACTGGTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAg                                                                                                                                   <  2:2061095/148‑1 (MQ=255)
             tATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGACACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCg                                                                                                                                >  2:378566/1‑148 (MQ=255)
                cGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTa                                                                                                                             <  1:3161645/148‑1 (MQ=255)
                cGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTa                                                                                                                             >  1:2848222/1‑148 (MQ=255)
                cGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTa                                                                                                                             >  1:1067620/1‑148 (MQ=255)
                   tCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAAct                                                                                                                          >  1:1141245/1‑148 (MQ=255)
                   tCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAAct                                                                                                                          >  1:3252999/1‑148 (MQ=255)
                    cAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAActc                                                                                                                         <  2:2080162/148‑1 (MQ=255)
                          aTCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAg                                                                                                                   <  2:205417/148‑1 (MQ=255)
                          aTCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAg                                                                                                                   >  2:2910181/1‑148 (MQ=255)
                           tCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGa                                                                                                                  >  1:1017873/1‑148 (MQ=255)
                           tCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGa                                                                                                                  <  2:1043539/148‑1 (MQ=255)
                             gAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtg                                                                                                                <  1:275530/148‑1 (MQ=255)
                                 tCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCt                                                                                                            >  1:1856492/1‑148 (MQ=255)
                                 tCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCt                                                                                                            >  2:2479845/1‑148 (MQ=255)
                                  cGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTg                                                                                                           >  1:2470201/1‑148 (MQ=255)
                                    actactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGa                                                                                                         <  1:805470/148‑1 (MQ=255)
                                      tactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa                                                                                                       >  2:2453367/1‑148 (MQ=255)
                                      tactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa                                                                                                       <  1:2637252/148‑1 (MQ=255)
                                      tactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa                                                                                                       <  1:1492967/148‑1 (MQ=255)
                                        ctGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATc                                                                                                     >  2:706134/1‑148 (MQ=255)
                                         tGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCg                                                                                                    <  2:160548/148‑1 (MQ=255)
                                           ttGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTg                                                                                                  <  2:2298443/148‑1 (MQ=255)
                                            tGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGc                                                                                                 >  1:1622923/1‑148 (MQ=255)
                                            tGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGc                                                                                                 >  1:679232/1‑148 (MQ=255)
                                               gTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTa                                                                                              <  1:914314/148‑1 (MQ=255)
                                                    cGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCgag                                                                                         >  1:265671/1‑148 (MQ=255)
                                                        tCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGc                                                                                     <  2:359482/148‑1 (MQ=255)
                                                                 cTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCg                                                                            <  2:2978380/148‑1 (MQ=255)
                                                                   gcgcGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGtt                                                                          >  1:1150435/1‑148 (MQ=255)
                                                                   gcgcGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGtt                                                                          >  1:3302100/1‑148 (MQ=255)
                                                                      cgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATc                                                                       <  1:757035/148‑1 (MQ=255)
                                                                      cgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATc                                                                       <  1:760406/148‑1 (MQ=255)
                                                                        aaGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGt                                                                     >  2:1856868/1‑148 (MQ=255)
                                                                         aGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc                                                                    >  1:2038269/1‑148 (MQ=255)
                                                                         aGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATAGAGAAGCCGAAAGGCGTTATCGTc                                                                    >  2:3093039/1‑148 (MQ=255)
                                                                           aCGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaa                                                                                 <  1:3440506/133‑1 (MQ=255)
                                                                           aCGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaa                                                                                 >  2:3440506/1‑133 (MQ=255)
                                                                           aCGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCa                                                                  >  1:2941940/1‑148 (MQ=255)
                                                                               ttACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAc                                                              >  1:435499/1‑148 (MQ=255)
                                                                                tACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcg                                                             <  2:1772039/148‑1 (MQ=255)
                                                                                  cGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcggc                                                           >  2:2374878/1‑148 (MQ=255)
                                                                                       ccATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATAGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCa                                                      >  1:3425685/1‑148 (MQ=255)
                                                                                        cATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAg                                                     >  1:2111057/1‑148 (MQ=255)
                                                                                         aTTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGa                                                    >  1:2060778/1‑148 (MQ=255)
                                                                                           tATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcc                                                  >  2:2286082/1‑148 (MQ=255)
                                                                                           tATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcc                                                  >  2:2160595/1‑148 (MQ=255)
                                                                                             tGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcccc                                                >  2:1017704/1‑148 (MQ=255)
                                                                                               gTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGc                                              >  1:2226823/1‑148 (MQ=255)
                                                                                                 tAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTg                                            >  1:154034/1‑148 (MQ=255)
                                                                                                   aCTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaa                                          >  1:1367358/1‑148 (MQ=255)
                                                                                                    cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaaa                                         >  2:3476463/1‑148 (MQ=255)
                                                                                                    cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaaa                                         >  1:2526374/1‑148 (MQ=255)
                                                                                                     tGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                        >  2:889598/1‑148 (MQ=255)
                                                                                                     tGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                        <  1:1355871/148‑1 (MQ=255)
                                                                                                     tGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                        >  2:3038957/1‑148 (MQ=255)
                                                                                                     tGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAGCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                        >  2:1262873/1‑148 (MQ=255)
                                                                                                      gTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACt                                       >  1:1146745/1‑148 (MQ=255)
                                                                                                         aCCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgc                                    >  1:1072226/1‑148 (MQ=255)
                                                                                                         aCCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgc                                    >  2:3440455/1‑148 (MQ=255)
                                                                                                          cccGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcg                                   >  2:2664753/1‑148 (MQ=255)
                                                                                                             ggAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgc                                >  1:2749163/1‑148 (MQ=255)
                                                                                                                aaCCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcgcg                             <  1:35863/148‑1 (MQ=255)
                                                                                                                aaCCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcgcg                             <  1:1644839/148‑1 (MQ=255)
                                                                                                                 aCCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcgcgc                            >  1:1000166/1‑148 (MQ=255)
                                                                                                                  ccGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCt                           >  2:3313790/1‑148 (MQ=255)
                                                                                                                   cGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTg                          >  2:3128015/1‑148 (MQ=255)
                                                                                                                     tctcCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGa                        <  1:42207/148‑1 (MQ=255)
                                                                                                                     tctcCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGa                        <  1:1852427/148‑1 (MQ=255)
                                                                                                                      ctcCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGaa                       >  1:519094/1‑148 (MQ=255)
                                                                                                                       tcCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAg                      <  2:1017873/148‑1 (MQ=255)
                                                                                                                       tcCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAg                      <  2:2828127/148‑1 (MQ=255)
                                                                                                                        ccACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgc                     <  2:2563094/148‑1 (MQ=255)
                                                                                                                          aCCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctg                   >  2:3315136/1‑148 (MQ=255)
                                                                                                                              aCTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTgg               >  2:3296352/1‑148 (MQ=255)
                                                                                                                               cTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGc              >  1:2420242/1‑148 (MQ=255)
                                                                                                                               cTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGc              >  1:629326/1‑148 (MQ=255)
                                                                                                                                     cacTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCg        >  2:285675/1‑148 (MQ=255)
                                                                                                                                      acTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCgg       >  2:3208108/1‑148 (MQ=255)
                                                                                                                                      acTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCgg       <  1:2614963/148‑1 (MQ=255)
                                                                                                                                        ttCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGtt     >  1:3096690/1‑148 (MQ=255)
                                                                                                                                         tCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTa    <  1:1747221/148‑1 (MQ=255)
                                                                                                                                         tCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTa    <  1:1747179/148‑1 (MQ=255)
                                                                                                                                         tCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTa    <  2:2000578/148‑1 (MQ=255)
                                                                                                                                           cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTAta  <  1:460092/148‑2 (MQ=255)
                                                                                                                                           cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTAta  <  2:2749163/148‑2 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GCGGCCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATC  >  CP009273/32516‑32802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑