Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,906,384 G→T 100% I215I (ATC→ATA)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,906,3840GT97.3% 603.0 / 6.1 198I215I (ATC→ATA) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base T (120/72);  total (125/73)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

GCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCC  >  CP009273/3906241‑3906528
                                                                                                                                               |                                                                                                                                                
gcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAttt                                                                                                                                              <  1:179917/148‑1 (MQ=255)
   ttCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCg                                                                                                                                           <  1:3236504/148‑1 (MQ=255)
    tCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGc                                                                                                                                          >  2:3165133/1‑148 (MQ=255)
      ggCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCaa                                                                                                                                        <  2:446727/148‑1 (MQ=255)
        cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata                                                                                                                                      <  1:2332146/148‑1 (MQ=255)
        cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata                                                                                                                                      <  2:559085/148‑1 (MQ=255)
         tGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                     >  2:1403955/1‑148 (MQ=255)
         tGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                     >  1:1897510/1‑148 (MQ=255)
           ttCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCg                                                                                                                                   <  2:1223565/148‑1 (MQ=255)
            tCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGACGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGc                                                                                                                                  >  2:3281132/1‑148 (MQ=255)
               tAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCct                                                                                                                               <  2:2814622/148‑1 (MQ=255)
               tAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCct                                                                                                                               >  2:121095/1‑148 (MQ=255)
                aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                              >  1:1576853/1‑148 (MQ=255)
                aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                              <  1:1822587/148‑1 (MQ=255)
                 gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctct                                                                                                                             <  1:327361/148‑1 (MQ=255)
                 gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctct                                                                                                                             >  1:2186880/1‑148 (MQ=255)
                  aTCTCTTTCTGCATGTAGTGAAGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCtt                                                                                                                            >  2:2459994/1‑148 (MQ=255)
                    ctctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCa                                                                                                                          <  2:949591/148‑1 (MQ=255)
                       tttCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAgg                                                                                                                       <  1:778045/148‑1 (MQ=255)
                        ttCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGa                                                                                                                      >  1:2083998/1‑148 (MQ=255)
                          cTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAg                                                                                                                    <  1:3093759/148‑1 (MQ=255)
                          cTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAg                                                                                                                    <  2:205960/148‑1 (MQ=255)
                             cATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATa                                                                                                                 <  2:2646203/148‑1 (MQ=255)
                             cATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATa                                                                                                                 <  2:435995/148‑1 (MQ=255)
                             cATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATa                                                                                                                 <  2:1124914/148‑1 (MQ=255)
                              aTGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaa                                                                                                                <  2:1001854/148‑1 (MQ=255)
                               tGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaaa                                                                                                               <  1:1496218/148‑1 (MQ=255)
                               tGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaaa                                                                                                               >  2:2872032/1‑148 (MQ=255)
                                gTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAg                                                                                                              >  2:3557744/1‑148 (MQ=255)
                                  aGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCg                                                                                                            <  2:1875904/148‑1 (MQ=255)
                                  aGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCg                                                                                                            >  2:1857754/1‑148 (MQ=255)
                                  aGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCg                                                                                                            <  2:3017439/148‑1 (MQ=255)
                                  aGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCg                                                                                                            >  2:3482048/1‑148 (MQ=255)
                                     gACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACg                                                                                                         >  2:166806/1‑148 (MQ=255)
                                     gACGGTAAAGGCCTTTATCGCCCGCGTCATATTGCAGTTTGGATTCGATATCCTGACTTTTTACTTCCGCGCCAGTTTTATCTAAGATGTTTACCGAACGGCGTGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACg                                                                                                         <  2:1943168/148‑1 (MQ=255)
                                              tGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCgg                                                                                                >  1:1494126/1‑148 (MQ=255)
                                              tGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCgg                                                                                                >  1:1493980/1‑148 (MQ=255)
                                                ccTTTATCGCCCGCGTCATTTTGCAGTTTGGATTCTATATCCTGACGTTTTACTTCCGCGCCTGTTTTATCTATGTTGTTTACCGAACGGCGAGTTTTTTCCGCAATAGCGCCCTCTTCACGTAATATAACGCGACGGGTCACCGGCa                                                                                              <  2:802589/148‑1 (MQ=255)
                                                  tttATCGCCCGGTTCTTATTGAAGTTTGGATTCTATATCCTGACTTTTTACTTCCGCGCCTGTTTTATCGATTATGTTTCCCGAACGGCGTGTTATTTCCGCAATATCGCCCTCTTCAAGTAAGAAAAAGCGACGGGTCACCGGCAAc                                                                                            <  2:107214/148‑1 (MQ=255)
                                                      tCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAgcg                                                                                        >  2:78353/1‑148 (MQ=255)
                                                           cgcgTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGc                                                                                   >  2:2512628/1‑148 (MQ=255)
                                                             cgTCATATTGCAGATTGGATTCTATATCCTGAGGTTTAACTTCCGCGCCAGTTTTATCGAATATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTg                                                                                 <  2:2271667/148‑1 (MQ=255)
                                                               tCATATTGCAGATTGTATTCGATAACCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGt                                                                               <  2:1999999/148‑1 (MQ=255)
                                                                cATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTc                                                                              >  2:1661595/1‑148 (MQ=255)
                                                                 atatTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCa                                                                             >  1:1747370/1‑148 (MQ=255)
                                                                   atTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa                                                                           >  2:995751/1‑148 (MQ=255)
                                                                        agtttGGATTCGATATCCTGACTTTTTACTTCCGCGCATGTTTTATCGAAGATGTTTACCGAACGGCGTGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGa                                                                      <  2:3129643/145‑1 (MQ=255)
                                                                          aTTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATa                                                                    >  2:1776046/1‑148 (MQ=255)
                                                                            tGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaaa                                                                  >  2:420771/1‑148 (MQ=255)
                                                                            tGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaaa                                                                  >  2:1547987/1‑148 (MQ=255)
                                                                             gttttCGATATCCTGCCTTTTTACTTCCGGCCCAGTTTTATCGATTTTGTTTACCGACCGGGGAGTTATTCCCCAAATATCGCCCTCTTCAAGGAAGATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAg                                                                 <  1:2325301/145‑1 (MQ=255)
                                                                              gATTCGATAGCCGGACGTTTTCCTGCCGCGCCCGTTTTATCGATGTTTTTTACCGACCGGCGAGTTATTGCCGCAATATCGCCCTCTTCACGGACGATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGt                                                                <  2:1623280/148‑1 (MQ=255)
                                                                                ttCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGttt                                                              >  1:3533124/1‑148 (MQ=255)
                                                                                 tCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt                                                             >  1:739658/1‑148 (MQ=255)
                                                                                    atatCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGc                                                          >  2:2908045/1‑148 (MQ=255)
                                                                                       tCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCa                                                       >  2:2377423/1‑148 (MQ=255)
                                                                                        ccttagtttttACTTCCGCCCCATTTTAATCTATTTTGTTTACCGAACGGCTTTTTTTTTCCGAAATATCGCCCTCATAAAGGAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCAt                                                      <  1:3528546/141‑1 (MQ=255)
                                                                                        ccTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAATTTTTCGCCCAt                                                      >  1:3514954/1‑148 (MQ=255)
                                                                                         gtGACTTTTTACTCCCGCGCCAGTTTTATCGAATTTGTTTACCGACGGGCGAGTTATTCCCCCAATACCGCCCTCTACAAGGAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATc                                                     <  2:123239/147‑1 (MQ=255)
                                                                                          tGACTTTTTACTTCCGCGCCTTTTTTATCTATTTTTTTTCCCTAAGGGCGTGTGTTTTCCGCATTATCGCCCTCTACATGTAATATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcc                                                    <  1:286454/148‑1 (MQ=255)
                                                                                           gACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATccc                                                   >  1:945962/1‑148 (MQ=255)
                                                                                           gACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATccc                                                   >  2:2660520/1‑148 (MQ=255)
                                                                                                tttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGc                                              >  1:5587/1‑148 (MQ=255)
                                                                                                tttACTGCCGCGCCAGGTTTATCGAAGATGTTTTCCGAACGGCGAGTTATGTCCGCAATATCGCCCTCTTGAGGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGCTCATGTGTGCGCCCATGCCCAGGc                                              >  2:2141934/1‑148 (MQ=255)
                                                                                                 ttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGcc                                             >  2:2580772/1‑148 (MQ=255)
                                                                                                  tACTTCCGCGCCAGTTTTATCGAATTTTTTTACCTAACGGCGATTTTTTTCGGCAATATCGCCCTCTTCAAGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCa                                            <  2:103283/148‑1 (MQ=255)
                                                                                                     ttCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATc                                         >  2:744021/1‑148 (MQ=255)
                                                                                                     ttCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATc                                         >  2:3083054/1‑148 (MQ=255)
                                                                                                      tCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCa                                        >  2:1132489/1‑148 (MQ=255)
                                                                                                      tCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCa                                        >  1:1238311/1‑148 (MQ=255)
                                                                                                      tCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCa                                        >  2:2944209/1‑148 (MQ=255)
                                                                                                        cgcgCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAcc                                      >  1:482368/1‑148 (MQ=255)
                                                                                                        cgcgCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAcc                                      >  1:2274004/1‑148 (MQ=255)
                                                                                                            ccAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCg                                  >  1:2778830/1‑148 (MQ=255)
                                                                                                             cAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCgg                                 >  1:92288/1‑148 (MQ=255)
                                                                                                              tgTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGa                                <  2:3493528/147‑1 (MQ=255)
                                                                                                              aGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGa                                >  2:1355309/1‑148 (MQ=255)
                                                                                                                ttttttCTAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                              <  2:2052463/148‑1 (MQ=255)
                                                                                                                 tttATCGAAGATTTTTACCTACCGGCGTGTTATTTCCGCAATATCGCCCTCTTCAAGGAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTa                             <  2:2083998/148‑1 (MQ=255)
                                                                                                                 tttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTa                             >  1:835963/1‑148 (MQ=255)
                                                                                                                  ttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCGCCAGGCCAATCACCAGCGGACTAc                            >  1:2500610/1‑148 (MQ=255)
                                                                                                                    aTCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCa                          >  1:2129434/1‑148 (MQ=255)
                                                                                                                    aTCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCa                          >  1:328225/1‑148 (MQ=255)
                                                                                                                     tCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAg                         >  1:2976214/1‑148 (MQ=255)
                                                                                                                          tttGTTTACCTAACGGCTATTTATTCCCGAATAAACGCCCTATTCAAGTAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGt                    <  1:1403955/146‑1 (MQ=255)
                                                                                                                           atttttACCGAACGGCTAGTTATTTCCGCAATAGCGCCCTCTACAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTg                   <  2:945939/145‑1 (MQ=255)
                                                                                                                           aTGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTg                   >  2:3321936/1‑148 (MQ=255)
                                                                                                                           aTGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTg                   >  1:1675095/1‑148 (MQ=255)
                                                                                                                             gTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc                 >  2:1914651/1‑148 (MQ=255)
                                                                                                                             gTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc                 >  1:1531003/1‑148 (MQ=255)
                                                                                                                                 aCCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCca             >  1:1142632/1‑148 (MQ=255)
                                                                                                                                 aCCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCca             >  1:1142750/1‑148 (MQ=255)
                                                                                                                                  ccGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcag            >  2:3216070/1‑148 (MQ=255)
                                                                                                                                     aaCGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcagcag         >  1:2986581/1‑148 (MQ=255)
                                                                                                                                      aCGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAgg        >  2:2055784/1‑148 (MQ=255)
                                                                                                                                       cGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAggg       >  2:2825445/1‑148 (MQ=255)
                                                                                                                                         gCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTa     >  2:1563645/1‑148 (MQ=255)
                                                                                                                                          cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt    >  1:2026911/1‑148 (MQ=255)
                                                                                                                                           gAGTTATTACCGCAATATCGCCCTCTTCAAGGAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATc   <  2:2186936/148‑1 (MQ=255)
                                                                                                                                            aGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc  >  2:1713427/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCC  >  CP009273/3906241‑3906528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑