Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,916,830 C→A 100% D71Y (GAT→TAT)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,916,8300CA99.5% 679.5 / ‑4.3 206D71Y (GAT→TAT) rsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (107/98);  total (107/99)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

GTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTG  >  CP009273/3916686‑3916974
                                                                                                                                                |                                                                                                                                                
gTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGAt                                                                                                                                               >  1:3207036/1‑148 (MQ=255)
gTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGAt                                                                                                                                               >  1:3392464/1‑148 (MQ=255)
 ttCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATa                                                                                                                                              >  1:2953850/1‑148 (MQ=255)
 ttCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATa                                                                                                                                              >  2:1916720/1‑148 (MQ=255)
  tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaa                                                                                                                                             <  2:2941043/148‑1 (MQ=255)
     atatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAAcc                                                                                                                                          >  2:1291014/1‑148 (MQ=255)
     atatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAAcc                                                                                                                                          >  1:3052986/1‑148 (MQ=255)
      tatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCg                                                                                                                                         >  2:2922448/1‑148 (MQ=255)
           tcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAc                                                                                                                                    <  1:589065/148‑1 (MQ=255)
            ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAcc                                                                                                                                   <  1:2039272/148‑1 (MQ=255)
                tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGc                                                                                                                               >  1:1309872/1‑148 (MQ=255)
                  tAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAg                                                                                                                             >  1:1674477/1‑148 (MQ=255)
                   aaGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGa                                                                                                                            <  1:1342735/148‑1 (MQ=255)
                      cTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATAc                                                                                                                         >  1:2455232/1‑148 (MQ=255)
                        cATGTTGCACCTGACGAAGGAAACGCACTCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       <  2:2729621/148‑1 (MQ=255)
                        cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       >  1:2082910/1‑148 (MQ=255)
                         aTGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGt                                                                                                                      <  2:640949/148‑1 (MQ=255)
                           gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                    >  1:3121898/1‑148 (MQ=255)
                           gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                    >  1:489380/1‑148 (MQ=255)
                            ttGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGcc                                                                                                                   >  1:184226/1‑148 (MQ=255)
                            ttGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGcc                                                                                                                   <  1:1208619/148‑1 (MQ=255)
                                 ccTGACGAAGTAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCcaccac                                                                                                              >  1:917131/1‑148 (MQ=255)
                                 ccTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCcaccac                                                                                                              >  2:225917/1‑148 (MQ=255)
                                  cTGATGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACcaca                                                                                                             <  1:1143272/148‑1 (MQ=255)
                                     aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                          <  1:225917/148‑1 (MQ=255)
                                     aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                          <  2:2863088/148‑1 (MQ=255)
                                     aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                          >  2:2989879/1‑148 (MQ=255)
                                      cGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAGTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACCTAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGc                                                                                                         >  2:1140856/1‑148 (MQ=255)
                                      cGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGc                                                                                                         <  2:2772153/148‑1 (MQ=255)
                                       gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCt                                                                                                        <  2:3038025/148‑1 (MQ=255)
                                         aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                      >  1:2504837/1‑148 (MQ=255)
                                         aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                      >  1:2406039/1‑148 (MQ=255)
                                          ggAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATc                                                                                                     >  1:891213/1‑148 (MQ=255)
                                          ggAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATc                                                                                                     >  2:1776788/1‑148 (MQ=255)
                                               cgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGaa                                                                                                >  2:3225900/1‑148 (MQ=255)
                                               cgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGaa                                                                                                <  1:1591553/148‑1 (MQ=255)
                                                 cacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAata                                                                                              <  2:132022/148‑1 (MQ=255)
                                                   cgcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATg                                                                                            >  2:2829718/1‑148 (MQ=255)
                                                    gcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATgg                                                                                           >  2:241890/1‑148 (MQ=255)
                                                    gcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATgg                                                                                           <  2:7155/148‑1 (MQ=255)
                                                    gcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATgg                                                                                           >  2:34741/1‑148 (MQ=255)
                                                    gcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATgg                                                                                           <  2:7388/148‑1 (MQ=255)
                                                     cgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGc                                                                                          >  2:908958/1‑148 (MQ=255)
                                                        ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTa                                                                                       >  1:653642/1‑148 (MQ=255)
                                                          aCCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTAcc                                                                                     >  2:1559013/1‑148 (MQ=255)
                                                               ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                                >  1:1536887/1‑148 (MQ=255)
                                                               ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                                <  2:446049/148‑1 (MQ=255)
                                                                   aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtctc                                                                             <  2:65312/147‑1 (MQ=255)
                                                                   aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCa                                                                            <  1:1120784/148‑1 (MQ=255)
                                                                         cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGa                                                                      <  1:903816/148‑1 (MQ=255)
                                                                            agTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                                   <  2:928167/148‑1 (MQ=255)
                                                                            agTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                                   >  1:1476162/1‑148 (MQ=255)
                                                                              tGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgcg                                                                 >  2:2028135/1‑148 (MQ=255)
                                                                               gAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCgg                                                                <  1:772529/148‑1 (MQ=255)
                                                                               gAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCgg                                                                >  2:3521474/1‑148 (MQ=255)
                                                                                aaaTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGa                                                               >  2:57920/1‑148 (MQ=255)
                                                                                       cTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGt                                                        <  1:2677549/148‑1 (MQ=255)
                                                                                         tCAGGACGCACGATAGAGAGTGGAATGCGTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATAGAGAATATGGCGTAACAGAATCTCATTAGGATCTAGGACCGAAGTCa                                                      <  2:1243771/148‑1 (MQ=255)
                                                                                           aGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAgg                                                    <  2:587441/148‑1 (MQ=255)
                                                                                            ggACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGt                                                   >  1:3291516/1‑148 (MQ=255)
                                                                                             gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGtt                                                  <  2:716224/148‑1 (MQ=255)
                                                                                             gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGtt                                                  >  2:323045/1‑148 (MQ=255)
                                                                                              aCGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTg                                                 <  2:3052986/148‑1 (MQ=255)
                                                                                                gCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTa                                               >  2:2401984/1‑148 (MQ=255)
                                                                                                 cACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTAc                                              >  2:623660/1‑148 (MQ=255)
                                                                                                   cGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGc                                            <  1:914662/148‑1 (MQ=255)
                                                                                                     aTAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATATCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCtt                                          >  2:3183844/1‑148 (MQ=255)
                                                                                                      tAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCttt                                         <  2:1470749/148‑1 (MQ=255)
                                                                                                      tAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCttt                                         >  2:3316096/1‑148 (MQ=255)
                                                                                                      tAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCttt                                         >  2:1723166/1‑148 (MQ=255)
                                                                                                       agagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTg                                        >  1:283129/1‑148 (MQ=255)
                                                                                                       agagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTg                                        <  1:291278/148‑1 (MQ=255)
                                                                                                         agagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGtt                                      >  2:2473266/1‑148 (MQ=255)
                                                                                                             tGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAt                                  >  1:3493138/1‑148 (MQ=255)
                                                                                                             tGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAt                                  <  2:2201019/148‑1 (MQ=255)
                                                                                                              ggAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAtt                                 >  2:2415211/1‑148 (MQ=255)
                                                                                                              ggAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAtt                                 <  1:2173526/148‑1 (MQ=255)
                                                                                                               gAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAttt                                <  2:1828323/148‑1 (MQ=255)
                                                                                                               gAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAttt                                <  2:1828240/148‑1 (MQ=255)
                                                                                                                 aTGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt                              <  2:3270062/148‑1 (MQ=255)
                                                                                                                        gCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCat                       <  1:2964430/148‑1 (MQ=255)
                                                                                                                        gCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCat                       >  1:1022219/1‑148 (MQ=255)
                                                                                                                         cAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata                      >  1:3003963/1‑148 (MQ=255)
                                                                                                                         cAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata                      >  1:3420691/1‑148 (MQ=255)
                                                                                                                           gTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATAtt                    >  1:2024165/1‑148 (MQ=255)
                                                                                                                           gTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATAtt                    <  2:1309872/148‑1 (MQ=255)
                                                                                                                           gTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATAtt                    <  1:2984380/148‑1 (MQ=255)
                                                                                                                            tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                   <  2:328124/148‑1 (MQ=255)
                                                                                                                            tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                   <  1:653027/148‑1 (MQ=255)
                                                                                                                             ccTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa                  <  1:261093/148‑1 (MQ=255)
                                                                                                                                ggTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGt               >  1:438800/1‑148 (MQ=255)
                                                                                                                                      gTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCaa         >  1:1253001/1‑148 (MQ=255)
                                                                                                                                      gTGCCGACATAGATAAACCGTTCACCGTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCaa         <  2:3415020/148‑1 (MQ=255)
                                                                                                                                       tGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAAt        <  1:34741/148‑1 (MQ=255)
                                                                                                                                          cGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAg     <  2:3420691/148‑1 (MQ=255)
                                                                                                                                          cGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAg     <  1:296688/148‑1 (MQ=255)
                                                                                                                                           gACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGc    >  2:2093168/1‑148 (MQ=255)
                                                                                                                                           gACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGc    <  2:2151549/148‑1 (MQ=255)
                                                                                                                                            aCATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCt   >  2:1868816/1‑148 (MQ=255)
                                                                                                                                             cATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTg  >  1:325490/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTG  >  CP009273/3916686‑3916974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑