Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,917,554 C→A 100% L480L (CTG→CTT)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,917,5540CA96.8% 640.8 / 9.0 215L480L (CTG→CTT) mnmG5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (123/84);  total (127/88)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 215 total aligned reads displayed.

ACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGC  >  CP009273/3917409‑3917697
                                                                                                                                                 |                                                                                                                                               
aCGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAg                                                                                                                                               <  1:3313951/148‑1 (MQ=255)
     gTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTCCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgct                                                                                                                                          <  1:246471/148‑1 (MQ=255)
       cAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                        >  2:509597/1‑148 (MQ=255)
        aGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctgac                                                                                                                                       >  2:2086927/1‑148 (MQ=255)
           gTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctgacgct                                                                                                                                    <  2:1644935/148‑1 (MQ=255)
            tGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctc                                                                                                                                   >  1:350270/1‑148 (MQ=255)
             ggTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGTGTTACCCAGGTCGATTTAAGACGCTGacgctca                                                                                                                                  >  1:3237489/1‑148 (MQ=255)
             ggTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGTGTTACCCAGGTCGATTTAAGACGCTGacgctca                                                                                                                                  >  1:1466250/1‑148 (MQ=255)
             ggTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctca                                                                                                                                  <  2:656833/148‑1 (MQ=255)
             ggTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGAGTTAAGACGCTGacgctca                                                                                                                                  >  1:2401494/1‑148 (MQ=255)
              gTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGAGTTAAGACGCTGacgctcac                                                                                                                                 >  2:2097246/1‑148 (MQ=255)
               ttAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacg                                                                                                                                >  2:488880/1‑148 (MQ=255)
               ttAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacg                                                                                                                                <  1:1204761/148‑1 (MQ=255)
                 aaTTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacgct                                                                                                                              <  1:1780587/148‑1 (MQ=255)
                   tttttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCg                                                                                                                            >  1:1395529/1‑148 (MQ=255)
                    ttttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGa                                                                                                                           >  2:719781/1‑148 (MQ=255)
                      ttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGata                                                                                                                         >  2:442040/1‑148 (MQ=255)
                       tCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGatat                                                                                                                        <  1:3211418/148‑1 (MQ=255)
                        cATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATAtt                                                                                                                       >  2:2707768/1‑148 (MQ=255)
                             gTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCaa                                                                                                                  >  2:402798/1‑148 (MQ=255)
                                aTTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGtt                                                                                                               >  1:999548/1‑148 (MQ=255)
                                  ttCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTtc                                                                                                             <  1:1022672/148‑1 (MQ=255)
                                     cGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCg                                                                                                          >  1:2350017/1‑148 (MQ=255)
                                        acgacgCAGCAGATCTTCACCACTGGCTTCACGGGAAATCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTa                                                                                                       >  1:3127646/1‑148 (MQ=255)
                                                gcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGTTCGATTTAAGAAGCTGACGCTCACGCTCGATATTCTCAAGTTTATCGTTAAAgcgcgc                                                                                               >  2:2210242/1‑148 (MQ=255)
                                                gcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcgc                                                                                               >  2:415181/1‑148 (MQ=255)
                                                gcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcgc                                                                                               <  2:1151607/148‑1 (MQ=255)
                                                gcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcgc                                                                                               >  2:2466067/1‑148 (MQ=255)
                                                 cagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGcc                                                                                              >  2:1811706/1‑148 (MQ=255)
                                                 cagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGcc                                                                                              <  1:74310/148‑1 (MQ=255)
                                                 cagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGcc                                                                                              >  1:682799/1‑148 (MQ=255)
                                                 cagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGcc                                                                                              >  2:2787536/1‑148 (MQ=255)
                                                  agATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGccc                                                                                             <  1:2898069/148‑1 (MQ=255)
                                                    aTCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa                                                                                           >  2:2655134/1‑148 (MQ=255)
                                                    aTCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa                                                                                           >  2:708431/1‑148 (MQ=255)
                                                        tCACCACTGGCTTCACGGGAAAGCGTCGCAGTCAGGTGAGCAGTCCCTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCGCGAGATTCTCGCGTTGCTCGTGAAAGCGCGCCCAACGtt                                                                                       >  2:2559746/1‑148 (MQ=255)
                                                        tCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGtt                                                                                       <  2:1494589/148‑1 (MQ=255)
                                                        tCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGtt                                                                                       >  2:2967059/1‑148 (MQ=255)
                                                        tCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGtt                                                                                       >  2:633795/1‑148 (MQ=255)
                                                         caccacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTc                                                                                      >  1:2255786/1‑148 (MQ=255)
                                                             acTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCa                                                                                  >  1:2807544/1‑148 (MQ=255)
                                                               tGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATc                                                                                <  1:3096044/148‑1 (MQ=255)
                                                                ggCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcc                                                                               <  2:741339/148‑1 (MQ=255)
                                                                 gCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcca                                                                              >  2:1907059/1‑148 (MQ=255)
                                                                   ttCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccacc                                                                            >  2:3346715/1‑148 (MQ=255)
                                                                   ttCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccacc                                                                            >  2:1825465/1‑148 (MQ=255)
                                                                    tCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccacca                                                                           <  1:1397413/148‑1 (MQ=255)
                                                                       cGGGACAGCGGCGCAGTCAGGTGAGAATTCACTTCGGCTGAAGCTTCAGCCGACGGGGTTACACAGGTCGATTGAATAACCTGACGATCACGCTCGCGATTCTCAAGTTTCTCGTTAAAGCGAGCCCAACGTTCGTCGGCCACCAgga                                                                        >  2:3471868/1‑147 (MQ=255)
                                                                         ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                      >  2:201818/1‑148 (MQ=255)
                                                                          gAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCa                                                                     >  2:2032279/1‑148 (MQ=255)
                                                                             aGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCCGtt                                                                  >  1:3005859/1‑148 (MQ=255)
                                                                             aGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGtt                                                                  >  1:2908431/1‑148 (MQ=255)
                                                                              gcggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTc                                                                 <  2:350270/148‑1 (MQ=255)
                                                                               cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCa                                                                <  1:3442335/148‑1 (MQ=255)
                                                                                 gcgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACg                                                              >  2:1497377/1‑148 (MQ=255)
                                                                                 gcgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACg                                                              >  2:3269005/1‑148 (MQ=255)
                                                                                    cAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAcc                                                           <  2:314615/148‑1 (MQ=255)
                                                                                     aGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCg                                                          <  1:2638673/148‑1 (MQ=255)
                                                                                      gTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGa                                                         <  2:2179545/148‑1 (MQ=255)
                                                                                       tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                        <  2:2992998/148‑1 (MQ=255)
                                                                                       tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                        >  2:3117557/1‑148 (MQ=255)
                                                                                       tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                        >  2:3128638/1‑148 (MQ=255)
                                                                                       tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                        >  1:3466255/1‑148 (MQ=255)
                                                                                        cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAtt                                                       >  1:2505025/1‑148 (MQ=255)
                                                                                        cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAtt                                                       >  1:3533508/1‑148 (MQ=255)
                                                                                             gAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGt                                                  <  2:2754533/148‑1 (MQ=255)
                                                                                              aGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTAGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTc                                                 >  2:1685170/1‑148 (MQ=255)
                                                                                                cATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCaa                                               >  2:1454109/1‑148 (MQ=255)
                                                                                                cATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGAGTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCaa                                               >  1:425675/1‑148 (MQ=255)
                                                                                                  ttCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTAACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTAAGTCaatc                                             >  1:2920191/1‑146 (MQ=255)
                                                                                                      cTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAg                                         <  2:3448642/148‑1 (MQ=255)
                                                                                                       ttCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                        >  2:1324398/1‑148 (MQ=255)
                                                                                                       ttCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                        >  2:2723154/1‑148 (MQ=255)
                                                                                                       ttCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGAGTTAAGAAGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                        >  1:1168502/1‑148 (MQ=255)
                                                                                                          ggCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATcc                                     >  1:2315962/1‑148 (MQ=255)
                                                                                                            cTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGc                                   >  2:3103071/1‑148 (MQ=255)
                                                                                                            cTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGc                                   >  1:2842012/1‑148 (MQ=255)
                                                                                                             tGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCa                                  <  1:1739349/148‑1 (MQ=255)
                                                                                                              gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAt                                 >  2:1035532/1‑148 (MQ=255)
                                                                                                               cAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGc                                                                        >  2:2911914/1‑108 (MQ=255)
                                                                                                               cAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGc                                                                        <  1:2911914/108‑1 (MQ=255)
                                                                                                                 gCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTAt                              <  1:206687/148‑1 (MQ=255)
                                                                                                                   ttCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCt                            >  2:3477843/1‑148 (MQ=255)
                                                                                                                    tCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCtt                           <  1:1957593/148‑1 (MQ=255)
                                                                                                                    tCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCtt                           <  1:2785250/148‑1 (MQ=255)
                                                                                                                         cgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGt                      <  1:986527/148‑1 (MQ=255)
                                                                                                                                 ttACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAg              <  2:1835501/148‑1 (MQ=255)
                                                                                                                                 ttACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAg              >  2:1364011/1‑148 (MQ=255)
                                                                                                                                 ttACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAg              <  1:216176/148‑1 (MQ=255)
                                                                                                                                     ccAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGa          <  2:3533508/148‑1 (MQ=255)
                                                                                                                                     ccAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGa          <  1:488880/148‑1 (MQ=255)
                                                                                                                                     ccAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGa          <  2:1395529/148‑1 (MQ=255)
                                                                                                                                      cAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGat         <  1:475365/148‑1 (MQ=255)
                                                                                                                                      cAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGat         >  1:875535/1‑148 (MQ=255)
                                                                                                                                      cAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGat         >  1:875834/1‑148 (MQ=255)
                                                                                                                                      cAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGat         <  1:154054/148‑1 (MQ=255)
                                                                                                                                       aGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGata        <  2:682799/148‑1 (MQ=255)
                                                                                                                                         gTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAAGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATAtt      >  2:1203690/1‑148 (MQ=255)
                                                                                                                                          tCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTc     <  2:525613/148‑1 (MQ=255)
                                                                                                                                             aTTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTgc  >  1:1687209/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGC  >  CP009273/3917409‑3917697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑