| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,958,885 | G→T | 6.2% | Q36K (CAG→AAG) | rhlB ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,958,885 | 0 | G | T | 6.2% | 882.1 / 20.8 | 241 | Q36K (CAG→AAG) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (106/120); new base T (9/6); total (115/126) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
Only 100 of 241 total aligned reads displayed.
CTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGC > CP009273/3958739‑3959030 | tGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGaa < 2:2971990/148‑1 (MQ=255) ttCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGACTgg > 2:2863797/1‑148 (MQ=255) ttCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATgg > 2:2316405/1‑148 (MQ=255) tCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATggg < 2:2056512/148‑1 (MQ=255) aCCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAACCGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCg < 2:707342/148‑1 (MQ=255) aCCTGGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGGTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCg < 2:2646660/148‑1 (MQ=255) cgtGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTa < 2:1855252/146‑1 (MQ=255) cTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTa > 1:850338/1‑148 (MQ=255) tGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACa > 1:459444/1‑148 (MQ=255) gCGATCGGCAAGCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCTGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAg > 1:1043916/1‑148 (MQ=255) cGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGt < 1:3105711/148‑1 (MQ=255) aTCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTa > 1:1455846/1‑148 (MQ=255) aTCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTa < 1:1851837/148‑1 (MQ=255) ggCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGa < 2:3008980/148‑1 (MQ=255) gCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGaa < 2:769379/148‑1 (MQ=255) aTCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAAcc > 1:2738763/1‑148 (MQ=255) cGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑t > 2:3552734/1‑148 (MQ=255) cGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑t > 1:2953677/1‑148 (MQ=255) ggATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑ttttt < 1:313936/148‑1 (MQ=255) gATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑tttttt < 2:1875668/148‑1 (MQ=255) aTGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑ttttttt > 1:109125/1‑148 (MQ=255) gagagagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑ttttttttt > 1:563916/1‑147 (MQ=255) gagagagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑ttttttttt > 1:3531325/1‑147 (MQ=255) agagagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCa > 1:1590459/1‑148 (MQ=255) gagagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑tttttttttca > 1:1010552/1‑145 (MQ=255) gagagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCaa > 1:283806/1‑148 (MQ=255) agagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCTGAAGGCCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑tttttttttcaa < 2:1050982/148‑5 (MQ=255) gaagaTAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCTGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGc < 2:779319/148‑1 (MQ=255) gaTAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTc > 2:2461210/1‑148 (MQ=255) aTAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCt > 1:2997662/1‑148 (MQ=255) tAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCt > 2:2846142/1‑148 (MQ=255) tGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACaa > 2:2216602/1‑148 (MQ=255) aaaCGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAAc < 1:2495170/148‑1 (MQ=255) aaaCGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAAcc > 2:3253796/1‑148 (MQ=255) aaCGTTGCCGTAAGAAACGCCATCGTTTTCCCGGGACCGGGTTGCGCCGGCCCGGCTACGTCACGCCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTTTCAAGGGCTTCTACAAc < 1:1283730/148‑1 (MQ=255) aaCGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAAcc > 2:3322786/1‑148 (MQ=255) aaCGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCt > 1:960925/1‑148 (MQ=255) cGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCtt < 2:3102643/148‑1 (MQ=255) aCGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATg < 2:2968144/148‑1 (MQ=255) tAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCGGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCa < 2:591774/148‑1 (MQ=255) aaGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAg > 2:2531348/1‑148 (MQ=255) aaGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAg > 2:1501585/1‑148 (MQ=255) aGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTTAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAggg > 1:2702521/1‑148 (MQ=255) gAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAggg > 1:1181562/1‑148 (MQ=255) aaCGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCg > 1:1373338/1‑148 (MQ=255) aCGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGa > 1:2018985/1‑148 (MQ=255) aCGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGaa > 2:862051/1‑148 (MQ=255) ccATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGt < 1:1084530/148‑1 (MQ=255) ccATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTc > 1:2025171/1‑148 (MQ=255) aTCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCgg > 1:3356863/1‑148 (MQ=255) aTCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCgg > 1:3356904/1‑148 (MQ=255) cGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGa > 1:2461695/1‑148 (MQ=255) ttttCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaaaa > 2:420252/1‑148 (MQ=255) tttCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaaaa < 1:838582/148‑1 (MQ=255) tttCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaaaa < 2:459444/148‑1 (MQ=255) cccGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACtt > 2:788402/1‑148 (MQ=255) ccGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttc < 2:160973/148‑1 (MQ=255) cGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctg < 1:714928/148‑1 (MQ=255) ggTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgt < 1:3394570/148‑1 (MQ=255) gTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGACACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttgtgt > 2:1914801/1‑148 (MQ=255) gTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgat > 2:1919075/1‑146 (MQ=255) tACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgtt > 1:1602348/1‑148 (MQ=255) aCCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct < 1:774494/148‑1 (MQ=255) ccGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct > 2:3084809/1‑148 (MQ=255) cGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttctg > 1:2148267/1‑148 (MQ=255) ttGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaa > 1:2305733/1‑148 (MQ=255) tGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaaa < 2:3174701/148‑1 (MQ=255) ccTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAtgtg > 2:716958/1‑148 (MQ=255) cTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATgtgt < 1:1960808/148‑1 (MQ=255) tGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGtt > 2:1210833/1‑148 (MQ=255) tGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGttt < 2:1150746/148‑1 (MQ=255) ggCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGtt < 2:1788650/147‑1 (MQ=255) ccGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTg < 2:563916/148‑1 (MQ=255) cTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCa > 1:3176624/1‑148 (MQ=255) aCGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAg > 1:3118459/1‑148 (MQ=255) cGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAg < 2:850338/148‑1 (MQ=255) cGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAg < 2:2119057/148‑1 (MQ=255) tCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAgtg > 1:3129213/1‑148 (MQ=255) cGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGt < 1:2612984/148‑1 (MQ=255) cGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGt < 1:382206/148‑1 (MQ=255) gACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGt < 1:2947048/148‑1 (MQ=255) gACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGt < 1:2680639/148‑1 (MQ=255) ccGCCAGCGTCAGCGGAAGGGCCAGTGCCTTAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGaa > 2:2932917/1‑148 (MQ=255) ccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGa < 2:1266806/148‑1 (MQ=255) cGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAat < 2:337887/148‑1 (MQ=255) ccAGCGTCAGCGGAAGGGCCAGTGCCTTAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAatat < 2:2702521/148‑1 (MQ=255) aGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATc > 2:1277619/1‑148 (MQ=255) cGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGc > 2:1749750/1‑148 (MQ=255) aGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTa < 1:1709361/148‑1 (MQ=255) gCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACt < 1:1464904/148‑1 (MQ=255) gtaGGGCCAGTGACTGAATGGGCGTACAGTTATTAAACCCT‑TTTTTTTTCAAGGGATTCTACAACCTTCTGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGATCATAGTGTGGTAGAATATCAGCTTACTAt < 1:51079/146‑1 (MQ=255) gAAGGGCCAGTGCCTGAATGGTCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTAt < 2:2948551/148‑1 (MQ=255) agtgCCAGTGCATGAATTGTCGTACATTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTg < 2:2503399/145‑1 (MQ=255) aGGGCCTGTGCCTGAATGGGCGTAAAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTg < 2:2013920/148‑1 (MQ=255) gggCCAGTGCCTTAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCt > 2:1471489/1‑148 (MQ=255) ggCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCtt > 2:2416736/1‑148 (MQ=255) gCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCttt > 1:234977/1‑148 (MQ=255) cAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTAc > 2:1391013/1‑148 (MQ=255) gCCTGAATGGGCTTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGATTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGaa < 2:3183846/148‑1 (MQ=255) cTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGc < 1:1281486/148‑1 (MQ=255) | CTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGC > CP009273/3958739‑3959030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |