Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,967,474 G→T 100% A169A (GCG→GCT)  rffH → glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,967,4740GT96.7% 562.2 / 7.6 182A169A (GCG→GCT) rffHglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (93/83);  total (97/85)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 185 total aligned reads displayed.

TCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGGCGC  >  CP009273/3967330‑3967618
                                                                                                                                                |                                                                                                                                                
tCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACACTTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCtgtg                                                                                                                                               <  2:3373650/148‑1 (MQ=255)
tCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCtgtg                                                                                                                                               <  2:3373195/148‑1 (MQ=255)
 cATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGa                                                                                                                                              <  2:3353659/148‑1 (MQ=255)
      tGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCggg                                                                                                                                         <  2:1335633/148‑1 (MQ=255)
        cggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCt                                                                                                                                       <  2:2677882/148‑1 (MQ=255)
         ggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCtt                                                                                                                                      <  1:1346067/148‑1 (MQ=255)
          gcgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCttt                                                                                                                                     >  1:3242262/1‑148 (MQ=255)
          gcgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCttt                                                                                                                                     >  1:2442066/1‑148 (MQ=255)
          gcgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCttt                                                                                                                                     <  2:2865952/148‑1 (MQ=255)
          gcgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCttt                                                                                                                                     >  2:1946346/1‑148 (MQ=255)
            gcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAt                                                                                                                                   <  1:2717527/148‑1 (MQ=255)
             cgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAtt                                                                                                                                  <  1:3151868/148‑1 (MQ=255)
             cgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAtt                                                                                                                                  <  1:2624668/148‑1 (MQ=255)
                   gAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACg                                                                                                                            >  2:1396619/1‑148 (MQ=255)
                   gAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACg                                                                                                                            >  2:1398022/1‑148 (MQ=255)
                    aaGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGa                                                                                                                           <  2:2353271/148‑1 (MQ=255)
                    aaGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGa                                                                                                                           >  1:320702/1‑148 (MQ=255)
                    aaGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGa                                                                                                                           >  1:320606/1‑148 (MQ=255)
                       gggCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAg                                                                                                                        <  1:2552022/148‑1 (MQ=255)
                       gggCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAg                                                                                                                        <  1:616519/148‑1 (MQ=255)
                        ggCGACGGTTTTTGTCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGt                                                                                                                       <  1:1237839/148‑1 (MQ=255)
                          cGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTaa                                                                                                                     <  1:433271/148‑1 (MQ=255)
                            aCGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAg                                                                                                                   <  1:3538210/148‑1 (MQ=255)
                            aCGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAg                                                                                                                   <  1:1776222/148‑1 (MQ=255)
                             cGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCTCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGt                                                                                                                  >  1:1574984/1‑148 (MQ=255)
                             cGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGt                                                                                                                  <  1:2052889/148‑1 (MQ=255)
                             cGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGt                                                                                                                  <  2:3506593/148‑1 (MQ=255)
                               gTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCg                                                                                                                <  1:1684837/148‑1 (MQ=255)
                                tttttGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGt                                                                                                               >  1:2078099/1‑148 (MQ=255)
                                      gCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTa                                                                                                         <  1:1116026/148‑1 (MQ=255)
                                       cTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTAc                                                                                                        >  2:1755828/1‑148 (MQ=255)
                                          tCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCa                                                                                                     >  1:2039509/1‑148 (MQ=255)
                                            aGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAGACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCaaa                                                                                                   <  2:738249/148‑1 (MQ=255)
                                             ggTGATGGACCCGGAACGCTTTGGCGTGGTGGCGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAg                                                                                                  <  2:1592645/148‑1 (MQ=255)
                                               tGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCa                                                                                                <  1:2398324/148‑1 (MQ=255)
                                                 aTGGACCCGGAACGCTTGGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAgg                                                                                              <  2:1547057/148‑1 (MQ=255)
                                                  tGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGt                                                                                             >  2:3428351/1‑148 (MQ=255)
                                                   ggACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTg                                                                                            >  2:1029222/1‑148 (MQ=255)
                                                    gACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGa                                                                                           >  1:3005159/1‑148 (MQ=255)
                                                      cccGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAg                                                                                         <  2:2966201/148‑1 (MQ=255)
                                                          gAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGt                                                                                     <  2:1914191/148‑1 (MQ=255)
                                                          gAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGt                                                                                     <  1:3224605/148‑1 (MQ=255)
                                                           aaCGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTc                                                                                    >  1:2346104/1‑148 (MQ=255)
                                                             cGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCgg                                                                                  <  2:2976436/148‑1 (MQ=255)
                                                               cTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAg                                                                                >  1:1396128/1‑148 (MQ=255)
                                                               cTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAg                                                                                <  2:497164/148‑1 (MQ=255)
                                                               cTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAg                                                                                >  2:1929522/1‑148 (MQ=255)
                                                                tttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGc                                                                               >  1:2205054/1‑148 (MQ=255)
                                                                         gtggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAAc                                                                      <  2:3182189/148‑1 (MQ=255)
                                                                         gtggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAAc                                                                      <  2:310724/148‑1 (MQ=255)
                                                                          tggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACt                                                                     >  2:2790117/1‑148 (MQ=255)
                                                                          tggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACt                                                                     >  2:3130726/1‑148 (MQ=255)
                                                                           ggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTg                                                                    >  1:3384272/1‑148 (MQ=255)
                                                                            gAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTgg                                                                   <  2:2296161/148‑1 (MQ=255)
                                                                            gAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTgg                                                                   >  2:3530832/1‑148 (MQ=255)
                                                                              gTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGgag                                                                 <  1:187624/148‑1 (MQ=255)
                                                                               tttGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGgaga                                                                >  1:1044182/1‑148 (MQ=255)
                                                                               tttGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGgaga                                                                >  1:130251/1‑148 (MQ=255)
                                                                               tttGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGgaga                                                                >  1:1929478/1‑148 (MQ=255)
                                                                                ttGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGAt                                                               >  2:2551602/1‑148 (MQ=255)
                                                                                  gacgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTa                                                             <  1:207497/148‑1 (MQ=255)
                                                                                   acgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAc                                                            <  1:936055/148‑1 (MQ=255)
                                                                                   acgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAc                                                            >  1:3428772/1‑148 (MQ=255)
                                                                                     gacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCt                                                          <  2:777778/148‑1 (MQ=255)
                                                                                      acAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTc                                                         <  2:1227374/148‑1 (MQ=255)
                                                                                       cAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTcc                                                        >  1:1943749/1‑148 (MQ=255)
                                                                                         aTTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCAt                                                      >  2:2147400/1‑148 (MQ=255)
                                                                                           ttCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCa                                                    >  2:3323538/1‑148 (MQ=255)
                                                                                            tCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCaa                                                   <  2:57206/148‑1 (MQ=255)
                                                                                              cgcgCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAAcc                                                 <  1:1594066/148‑1 (MQ=255)
                                                                                                cgcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAg                                               >  2:3025201/1‑148 (MQ=255)
                                                                                                cgcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAg                                               >  2:1247144/1‑148 (MQ=255)
                                                                                                cgcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAg                                               >  1:2270564/1‑148 (MQ=255)
                                                                                                 gcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGa                                              <  1:3491666/148‑1 (MQ=255)
                                                                                                 gcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGa                                              <  1:1860209/148‑1 (MQ=255)
                                                                                                  cTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGAt                                             >  2:269759/1‑148 (MQ=255)
                                                                                                  cTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGAt                                             >  2:3079969/1‑148 (MQ=255)
                                                                                                  cTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGAt                                             <  1:2173441/148‑1 (MQ=255)
                                                                                                   tATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATg                                            >  2:290968/1‑148 (MQ=255)
                                                                                                   tATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATg                                            <  2:2078099/148‑1 (MQ=255)
                                                                                                       tcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTAcc                                        >  1:3494220/1‑148 (MQ=255)
                                                                                                        cGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCt                                       <  2:2538113/148‑1 (MQ=255)
                                                                                                        cGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCt                                       >  1:678087/1‑148 (MQ=255)
                                                                                                        cGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCt                                       >  1:678084/1‑148 (MQ=255)
                                                                                                            ggAAGAAAAGCCAAAACAGCCGACGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAg                                   <  2:89843/148‑1 (MQ=255)
                                                                                                            ggAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAg                                   <  2:701124/148‑1 (MQ=255)
                                                                                                              aagaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGc                                 <  1:1422984/148‑1 (MQ=255)
                                                                                                               agaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCg                                >  2:2819968/1‑148 (MQ=255)
                                                                                                               agaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCg                                >  1:2034968/1‑148 (MQ=255)
                                                                                                                 aaaaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCggg                              >  1:1743980/1‑148 (MQ=255)
                                                                                                                   aaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCa                            >  2:3554093/1‑148 (MQ=255)
                                                                                                                   aaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCa                            <  1:2685111/148‑1 (MQ=255)
                                                                                                                            cAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCg                   >  1:816306/1‑148 (MQ=255)
                                                                                                                             aGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGt                  >  2:763844/1‑148 (MQ=255)
                                                                                                                                  aaGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAAc             <  1:1029222/148‑1 (MQ=255)
                                                                                                                                    gTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTg           <  2:2284339/148‑1 (MQ=255)
                                                                                                                                     tCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGc          >  2:537300/1‑148 (MQ=255)
                                                                                                                                         aCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCgg      >  1:3472245/1‑148 (MQ=255)
                                                                                                                                            gggCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGgcg   >  2:1160708/1‑148 (MQ=255)
                                                                                                                                             ggCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGgcgc  >  2:2356771/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGGCGC  >  CP009273/3967330‑3967618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑