Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,988,075 T→A 100% V65E (GTG→GAG)  yifL → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,988,0750TA99.1% 771.6 / ‑2.6 234V65E (GTG→GAG) yifLputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (107/124);  total (107/127)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 237 total aligned reads displayed.

CTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCT  >  CP009273/3987930‑3988219
                                                                                                                                                 |                                                                                                                                                
cTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGgaga                                                                                                                                                <  2:622804/148‑1 (MQ=255)
 tGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGaa                                                                                                                                               <  1:2789945/148‑1 (MQ=255)
 tGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGaa                                                                                                                                               <  2:2233073/148‑1 (MQ=255)
  gACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAAt                                                                                                                                              >  2:3344386/1‑148 (MQ=255)
       gCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTa                                                                                                                                         <  2:2332176/148‑1 (MQ=255)
            ggTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGt                                                                                                                                    <  1:514777/148‑1 (MQ=255)
            ggTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGt                                                                                                                                    <  2:1673751/148‑1 (MQ=255)
              tCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCACACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCa                                                                                                                                  <  1:181318/148‑1 (MQ=255)
              tCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCa                                                                                                                                  <  1:1023312/148‑1 (MQ=255)
               cTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAg                                                                                                                                 <  2:2452919/148‑1 (MQ=255)
                  aatggTGCGCTATATTTCCGGCCTGCAGATAAAAACTCACCGCCGCCGACCAAACAGGTTGAGACGAACACGCATTCCAGGGTTCCGTATAAAAACGCTCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGttt                                                                                                                              <  1:2545844/145‑1 (MQ=255)
                  aaaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGttt                                                                                                                              >  1:737118/1‑148 (MQ=255)
                   aaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTc                                                                                                                             <  2:2487914/148‑1 (MQ=255)
                    aGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCt                                                                                                                            <  2:1406831/148‑1 (MQ=255)
                     ggTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTg                                                                                                                           >  2:3348274/1‑148 (MQ=255)
                     ggTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTg                                                                                                                           >  2:2423985/1‑148 (MQ=255)
                      gTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCTATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGt                                                                                                                          >  1:3203164/1‑148 (MQ=255)
                       tCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACTCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTa                                                                                                                         <  2:2150362/148‑1 (MQ=255)
                         cGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTAcc                                                                                                                       <  2:1754188/148‑1 (MQ=255)
                            tctATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCcgc                                                                                                                    >  2:534073/1‑148 (MQ=255)
                                tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGa                                                                                                                <  2:3516818/148‑1 (MQ=255)
                                     cGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGa                                                                                                           <  1:2503282/148‑1 (MQ=255)
                                     cGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGa                                                                                                           <  1:2004770/148‑1 (MQ=255)
                                        cTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTa                                                                                                        <  1:1576445/148‑1 (MQ=255)
                                         tGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTaa                                                                                                       <  1:2730777/148‑1 (MQ=255)
                                          gCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTaaa                                                                                                      <  1:588684/148‑1 (MQ=255)
                                              aTAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAatgat                                                                                                  >  1:1310976/1‑148 (MQ=255)
                                                aaaaaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGc                                                                                                >  2:1042106/1‑148 (MQ=255)
                                                   aaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGt                                                                                             >  1:2188712/1‑148 (MQ=255)
                                                   aaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGt                                                                                             >  1:3369373/1‑148 (MQ=255)
                                                    aCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGtt                                                                                            <  1:436346/148‑1 (MQ=255)
                                                     cGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtc                                                                                           <  2:1439915/148‑1 (MQ=255)
                                                     cGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtc                                                                                           <  1:677730/148‑1 (MQ=255)
                                                     cGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtc                                                                                           <  2:74821/148‑1 (MQ=255)
                                                        aCCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCg                                                                                        <  2:1311781/148‑1 (MQ=255)
                                                        aCCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCg                                                                                        >  2:939193/1‑148 (MQ=255)
                                                         ccgccgccgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGa                                                                                       >  2:40035/1‑148 (MQ=255)
                                                           gccgccgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGaaa                                                                                     <  1:2333670/148‑1 (MQ=255)
                                                               ccgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGc                                                                                 <  2:97316/148‑1 (MQ=255)
                                                                cgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCa                                                                                <  1:1063634/148‑1 (MQ=255)
                                                                   ccAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATgg                                                                             <  1:1965781/148‑1 (MQ=255)
                                                                    cAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGc                                                                            >  2:1348885/1‑148 (MQ=255)
                                                                     aaaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGcc                                                                           <  1:3518116/148‑1 (MQ=255)
                                                                     aaaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGcc                                                                           <  1:1766067/148‑1 (MQ=255)
                                                                      aaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCt                                                                          <  1:1161523/148‑1 (MQ=255)
                                                                      aaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCt                                                                          <  1:3368585/148‑1 (MQ=255)
                                                                      aaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCt                                                                          <  2:288447/148‑1 (MQ=255)
                                                                       aCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCtt                                                                         <  2:3122180/148‑1 (MQ=255)
                                                                           gTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCa                                                                     >  1:373664/1‑148 (MQ=255)
                                                                            tAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCaa                                                                    <  2:2473720/148‑1 (MQ=255)
                                                                            tAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCaa                                                                    >  1:3265224/1‑148 (MQ=255)
                                                                              gagaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACg                                                                  <  1:749647/148‑1 (MQ=255)
                                                                              gagaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACg                                                                  <  1:749517/148‑1 (MQ=255)
                                                                                 acgcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtt                                                               >  2:1039314/1‑148 (MQ=255)
                                                                                  cgcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAttt                                                              <  2:1192176/148‑1 (MQ=255)
                                                                                   gcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtttt                                                             <  1:1848427/148‑1 (MQ=255)
                                                                                   gcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCGTGGCAACGAtttt                                                             <  1:1657219/148‑1 (MQ=255)
                                                                                       acgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAACGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATgg                                                         >  2:1267566/1‑148 (MQ=255)
                                                                                       acgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATgg                                                         >  1:3163553/1‑148 (MQ=255)
                                                                                       acgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATgg                                                         <  1:1379625/148‑1 (MQ=255)
                                                                                        cgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgt                                                        >  2:1083860/1‑148 (MQ=255)
                                                                                         gcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtc                                                       >  1:2947365/1‑148 (MQ=255)
                                                                                            aTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcgtc                                                    <  1:2084599/148‑1 (MQ=255)
                                                                                            aTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcgtc                                                    >  2:1974147/1‑148 (MQ=255)
                                                                                             tCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGtcgtcg                                                   >  1:842411/1‑148 (MQ=255)
                                                                                                    tGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGt                                            >  1:914152/1‑148 (MQ=255)
                                                                                                      ccGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTaa                                          <  2:528202/148‑1 (MQ=255)
                                                                                                       cGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAAc                                         >  1:339528/1‑148 (MQ=255)
                                                                                                           tAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAg                                     <  2:1855722/148‑1 (MQ=255)
                                                                                                            aaaaaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGa                                    >  1:127016/1‑148 (MQ=255)
                                                                                                              aaaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAAt                                  >  1:605022/1‑148 (MQ=255)
                                                                                                               aaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATg                                 <  2:475472/148‑1 (MQ=255)
                                                                                                               aaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATg                                 <  2:2270526/148‑1 (MQ=255)
                                                                                                                aCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGt                                <  2:3229358/148‑1 (MQ=255)
                                                                                                                aCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGt                                >  2:1528628/1‑148 (MQ=255)
                                                                                                                 cGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGACTGTc                               >  2:2415506/1‑148 (MQ=255)
                                                                                                                  gATCGCGCCACGGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCt                              <  1:2509172/148‑1 (MQ=255)
                                                                                                                    tCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCttt                            >  1:3057611/1‑148 (MQ=255)
                                                                                                                     cgcgCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAcgc                                               >  2:847482/1‑128 (MQ=255)
                                                                                                                     cgcgCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAcgc                                               >  2:2782309/1‑128 (MQ=255)
                                                                                                                     cgcgCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAcgc                                               <  1:847482/128‑1 (MQ=255)
                                                                                                                     cgcgCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                           >  1:2243031/1‑148 (MQ=255)
                                                                                                                     cgcgCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                           >  1:2243811/1‑148 (MQ=255)
                                                                                                                     cgcgCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                           >  2:1929161/1‑148 (MQ=255)
                                                                                                                     cgcgCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                           >  1:2633975/1‑148 (MQ=255)
                                                                                                                          cACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCa                      >  2:2304607/1‑148 (MQ=255)
                                                                                                                          cACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCa                      <  2:268908/148‑1 (MQ=255)
                                                                                                                           aCTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCAc                     >  2:663647/1‑148 (MQ=255)
                                                                                                                             tGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCg                   >  2:2829922/1‑148 (MQ=255)
                                                                                                                                 gATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGc               <  1:2003561/148‑1 (MQ=255)
                                                                                                                                 gATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGc               <  1:978196/148‑1 (MQ=255)
                                                                                                                                  aTGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCt              >  2:3083773/1‑148 (MQ=255)
                                                                                                                                      tCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGAtt          <  2:637591/148‑1 (MQ=255)
                                                                                                                                       ccATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtc         <  1:409852/148‑1 (MQ=255)
                                                                                                                                          tCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtcgtc      >  1:1886143/1‑148 (MQ=255)
                                                                                                                                           cccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtcgtcg     <  1:1474347/148‑1 (MQ=255)
                                                                                                                                            ccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGc    <  2:737118/148‑1 (MQ=255)
                                                                                                                                             cAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGcc   <  1:187792/148‑1 (MQ=255)
                                                                                                                                              aGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCt  >  1:335235/1‑148 (MQ=255)
                                                                                                                                              aGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCt  <  1:2058972/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCT  >  CP009273/3987930‑3988219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑