Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,994,247 A→T 100% V57E (GTG→GAG)  yigE ← DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,994,2470AT100.0% 761.7 / ‑6.7 233V57E (GTG→GAG) yigEDUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (109/122);  minor base G (0/1);  total (110/123)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

ATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCT  >  CP009273/3994103‑3994390
                                                                                                                                                |                                                                                                                                               
aTGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTccc                                                                                                                                              >  2:2269915/1‑148 (MQ=255)
 tGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCg                                                                                                                                             >  1:702950/1‑148 (MQ=255)
 tGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCg                                                                                                                                             >  2:1220503/1‑148 (MQ=255)
    aCAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGctc                                                                                                                                          >  1:1066221/1‑148 (MQ=255)
      aaaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTg                                                                                                                                        <  1:962777/148‑1 (MQ=255)
        aCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGtt                                                                                                                                      <  2:1978166/148‑1 (MQ=255)
        aCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGtt                                                                                                                                      <  2:1278527/148‑1 (MQ=255)
         ccGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCAGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGttt                                                                                                                                     >  1:2184631/1‑148 (MQ=255)
              cggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGa                                                                                                                                <  2:2989224/148‑1 (MQ=255)
                gcgcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAtt                                                                                                                              <  1:227146/148‑1 (MQ=255)
                  gcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTaa                                                                                                                            <  2:1782436/148‑1 (MQ=255)
                  gcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGGTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTaa                                                                                                                            <  2:1813754/148‑1 (MQ=255)
                    aTAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACg                                                                                                                          >  2:2514511/1‑148 (MQ=255)
                       gCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGta                                                                                                                       >  1:1777142/1‑148 (MQ=255)
                       gCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGta                                                                                                                       >  1:1777147/1‑148 (MQ=255)
                        cTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtat                                                                                                                      >  1:280752/1‑148 (MQ=255)
                           tcatcaTAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACg                                                                                                                   <  2:2620575/148‑1 (MQ=255)
                           tcatcaTAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACg                                                                                                                   <  2:2726530/148‑1 (MQ=255)
                              tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCt                                                                                                                >  2:28997/1‑148 (MQ=255)
                              tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCt                                                                                                                >  2:1260570/1‑148 (MQ=255)
                              tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGAATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCt                                                                                                                <  1:2634428/148‑1 (MQ=255)
                                 tAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTa                                                                                                             <  2:395975/148‑1 (MQ=255)
                                  aGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTAc                                                                                                            >  1:3072945/1‑148 (MQ=255)
                                  aGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTAc                                                                                                            >  2:2892624/1‑148 (MQ=255)
                                     tGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGt                                                                                                         <  1:1002653/148‑1 (MQ=255)
                                          ccgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCg                                                                                                    >  1:1207464/1‑148 (MQ=255)
                                          ccgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCg                                                                                                    <  1:865708/148‑1 (MQ=255)
                                           cgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGt                                                                                                   <  2:981451/148‑1 (MQ=255)
                                              tCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCgg                                                                                                >  2:3469967/1‑148 (MQ=255)
                                               cATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGa                                                                                               <  2:1967146/148‑1 (MQ=255)
                                               cATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGa                                                                                               <  1:3216696/148‑1 (MQ=255)
                                               cATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGa                                                                                               <  1:1448324/148‑1 (MQ=255)
                                                aTCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCCGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGAt                                                                                              <  2:891489/148‑1 (MQ=255)
                                                 tCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATc                                                                                             <  1:1292652/148‑1 (MQ=255)
                                                   gCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTg                                                                                           <  2:266488/148‑1 (MQ=255)
                                                     cATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTgag                                                                                         >  2:1472658/1‑148 (MQ=255)
                                                      aTCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTgaga                                                                                        <  2:3054687/148‑1 (MQ=255)
                                                        cTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGt                                                                                      >  2:2166891/1‑148 (MQ=255)
                                                          gCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTgc                                                                                    >  2:2357639/1‑148 (MQ=255)
                                                             cctgaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCa                                                                                 >  2:1116483/1‑148 (MQ=255)
                                                               tgaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAat                                                                               >  1:2772334/1‑148 (MQ=255)
                                                               tgaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAat                                                                               >  1:508102/1‑148 (MQ=255)
                                                                 accctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatca                                                                             >  1:1569125/1‑148 (MQ=255)
                                                                 accctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatca                                                                             <  2:731368/148‑1 (MQ=255)
                                                                  ccctgacTATTAATCTCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatcat                                                                            <  1:3393613/148‑1 (MQ=255)
                                                                   cctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatcatc                                                                           <  1:2892624/148‑1 (MQ=255)
                                                                   cctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatcatc                                                                           <  1:1180394/148‑1 (MQ=255)
                                                                   cctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatcatc                                                                           <  2:166039/148‑1 (MQ=255)
                                                                       acTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCg                                                                       >  2:848237/1‑148 (MQ=255)
                                                                         tATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGc                                                                     >  2:2527525/1‑148 (MQ=255)
                                                                          aTTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCa                                                                    <  2:2399625/148‑1 (MQ=255)
                                                                           ttAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCaa                                                                   <  1:3114506/148‑1 (MQ=255)
                                                                            tAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAAc                                                                  >  2:471582/1‑148 (MQ=255)
                                                                               tatCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAat                                                                               >  1:823126/1‑132 (MQ=255)
                                                                                atCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCa                                                              >  1:1353117/1‑148 (MQ=255)
                                                                                 tCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCaa                                                             <  1:605772/148‑1 (MQ=255)
                                                                                    gCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACa                                                          <  1:3508184/148‑1 (MQ=255)
                                                                                         cagAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGa                                                     <  2:672395/148‑1 (MQ=255)
                                                                                         cagAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGa                                                     <  2:1066221/148‑1 (MQ=255)
                                                                                          agagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGaa                                                    <  1:1472658/148‑1 (MQ=255)
                                                                                             gCATGTAACGTTCCCCACTCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTa                                                 <  2:2789006/148‑1 (MQ=255)
                                                                                             gCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTa                                                 >  1:1047401/1‑148 (MQ=255)
                                                                                             gCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTa                                                 <  1:2081663/148‑1 (MQ=255)
                                                                                               aTGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAg                                               <  2:2124382/148‑1 (MQ=255)
                                                                                                tGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAgg                                              <  1:1518694/148‑1 (MQ=255)
                                                                                                 gTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGt                                             >  1:3250045/1‑148 (MQ=255)
                                                                                                  tAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTg                                            <  2:280752/148‑1 (MQ=255)
                                                                                                  tAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTg                                            >  2:1046336/1‑148 (MQ=255)
                                                                                                   aaCGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGa                                           <  2:2279350/148‑1 (MQ=255)
                                                                                                   aaCGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGa                                           <  1:2038068/148‑1 (MQ=255)
                                                                                                     cGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAgg                                         >  2:777036/1‑148 (MQ=255)
                                                                                                      gTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAggg                                        >  2:2553088/1‑148 (MQ=255)
                                                                                                       ttCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGc                                       >  1:1848033/1‑148 (MQ=255)
                                                                                                             aCGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaa                                 <  1:1124373/148‑1 (MQ=255)
                                                                                                              cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaaa                                <  1:317546/148‑1 (MQ=255)
                                                                                                              cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaaa                                <  1:384536/148‑1 (MQ=255)
                                                                                                                 ttCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATc                             >  1:3522350/1‑148 (MQ=255)
                                                                                                                  tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATcc                            >  1:2078517/1‑148 (MQ=255)
                                                                                                                  tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATcc                            >  2:3501521/1‑148 (MQ=255)
                                                                                                                      cATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACATGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                        >  2:1096188/1‑148 (MQ=255)
                                                                                                                      cATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                        <  2:2312399/148‑1 (MQ=255)
                                                                                                                         tGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGACGTAAGGTGAGGGCGAGAAAAATCCGTTTgag                     >  2:1845628/1‑148 (MQ=255)
                                                                                                                         tGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTgag                     <  1:3020071/148‑1 (MQ=255)
                                                                                                                         tGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTgag                     <  1:603683/148‑1 (MQ=255)
                                                                                                                           gCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAt                   >  1:1111200/1‑148 (MQ=255)
                                                                                                                           gCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAt                   >  2:3344084/1‑148 (MQ=255)
                                                                                                                               tttGCCAGTACATTTTCTCCCTCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCTCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTTAGGGCGAGAAAAATCCGTTTGAGATTCaa               <  1:2305839/148‑1 (MQ=255)
                                                                                                                                 tGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAgg             <  2:3196688/148‑1 (MQ=255)
                                                                                                                                   ccAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTg           <  2:1991953/148‑1 (MQ=255)
                                                                                                                                    cAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGa          <  2:3280327/148‑1 (MQ=255)
                                                                                                                                     aGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGAt         <  2:3174131/148‑1 (MQ=255)
                                                                                                                                     aGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGAt         <  2:2553811/148‑1 (MQ=255)
                                                                                                                                      gTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATc        <  2:632416/148‑1 (MQ=255)
                                                                                                                                        aCATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAt      >  1:2491567/1‑148 (MQ=255)
                                                                                                                                        aCATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAt      >  1:430294/1‑148 (MQ=255)
                                                                                                                                        aCATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAt      >  2:1683315/1‑148 (MQ=255)
                                                                                                                                          aTTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTc    <  1:3416500/148‑1 (MQ=255)
                                                                                                                                           ttttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTcc   <  1:784862/148‑1 (MQ=255)
                                                                                                                                           ttttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTcc   >  2:2040117/1‑148 (MQ=255)
                                                                                                                                            tttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCt  >  1:2506802/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCT  >  CP009273/3994103‑3994390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑