Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,020,520 G→T 12.6% D212Y (GAT→TAT)  fre → NAD(P)H‑flavin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,020,5200GT12.6% 659.3 / 43.7 206D212Y (GAT→TAT) freNAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand):  ref base G (76/103);  new base T (16/11);  total (92/114)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

AGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGT  >  CP009273/4020375‑4020667
                                                                                                                                                 |                                                                                                                                                   
aGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGAt                                                                                                                                                   <  2:2345789/148‑1 (MQ=255)
aGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGAt                                                                                                                                                   <  1:1723931/148‑1 (MQ=255)
 gtggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATc                                                                                                                                                  >  2:1597598/1‑148 (MQ=255)
    gtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGt                                                                                                                                               <  2:484169/148‑1 (MQ=255)
    gtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGt                                                                                                                                               <  2:3408418/148‑1 (MQ=255)
      gCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGttt                                                                                                                                             <  2:3225674/148‑1 (MQ=255)
      gCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGttt                                                                                                                                             >  2:1840498/1‑148 (MQ=255)
        cGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTg                                                                                                                                           >  1:1933569/1‑148 (MQ=255)
          gtggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCa                                                                                                                                         >  2:2791718/1‑148 (MQ=255)
           tggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAg                                                                                                                                        >  2:112371/1‑148 (MQ=255)
               tGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAg                                                                                                                                    >  2:1896310/1‑148 (MQ=255)
                 aacaacCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCg                                                                                                                                  <  1:2993022/148‑1 (MQ=255)
                   caacCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTa                                                                                                                                >  1:2279171/1‑148 (MQ=255)
                   caacCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTa                                                                                                                                <  1:2187118/148‑1 (MQ=255)
                            gCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTGTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCggg                                                                                                                       >  1:1006638/1‑148 (MQ=255)
                             cGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGa                                                                                                                      <  2:1831617/148‑1 (MQ=255)
                               ggCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAg                                                                                                                    >  1:1239956/1‑148 (MQ=255)
                                gCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTTGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGa                                                                                                                   <  2:1319277/148‑1 (MQ=255)
                                   ggCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCg                                                                                                                <  1:2844861/148‑1 (MQ=255)
                                     cGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGcc                                                                                                              <  2:2018096/148‑1 (MQ=255)
                                     cGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTATTTTGCAGTGAGCGTAATGCGCGGGAAGATCGcc                                                                                                              <  1:2142581/148‑1 (MQ=255)
                                        gggCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGt                                                                                                           <  1:3125375/148‑1 (MQ=255)
                                          gCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTGTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAGTGCGCGGGAAGATCGCCTGttt                                                                                                         >  1:1375103/1‑148 (MQ=255)
                                            gTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTgg                                                                                                       <  1:2426076/148‑1 (MQ=255)
                                                tGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGAt                                                                                                   >  1:167195/1‑148 (MQ=255)
                                                tGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGAt                                                                                                   >  1:246773/1‑148 (MQ=255)
                                                tGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGAt                                                                                                   >  2:2420023/1‑148 (MQ=255)
                                                 ggCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATg                                                                                                  <  2:815598/148‑1 (MQ=255)
                                                  gCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGc                                                                                                 >  2:103878/1‑148 (MQ=255)
                                                     ccGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGtt                                                                                              <  2:257678/148‑1 (MQ=255)
                                                        tgtTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGc                                                                                           >  1:801309/1‑148 (MQ=255)
                                                          ttAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCAt                                                                                         <  1:1983251/148‑1 (MQ=255)
                                                          ttAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCAt                                                                                         >  1:775863/1‑148 (MQ=255)
                                                            aaCGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCAttt                                                                                       <  1:732701/148‑1 (MQ=255)
                                                             aCGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTa                                                                                      <  2:714326/148‑1 (MQ=255)
                                                              cggcggTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTAt                                                                                     <  2:775863/148‑1 (MQ=255)
                                                              cggcggTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTAt                                                                                     <  2:3141970/148‑1 (MQ=255)
                                                              cggcggTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTGTTGAGATGGCGAAAATTGCCCGCGATCTGGTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTAt                                                                                     >  1:513678/1‑148 (MQ=255)
                                                              cggcggGATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTAt                                                                                     <  2:2240479/148‑1 (MQ=255)
                                                                 cggTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTg                                                                                  <  1:2621278/148‑1 (MQ=255)
                                                                       tGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGAGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGAATTTATCTGAGatat                                                                            >  2:2173132/1‑148 (MQ=255)
                                                                           ggATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTGTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATaaaa                                                                        >  1:109813/1‑148 (MQ=255)
                                                                            gATCACGGTACGCTGGCAGAGCATGATATCTATATTGACGGACGTTTTGAGTTGGCGAAAATTTCACCCGATCTGTTTTGCCGTGAGAGTAATGAGCGGGCAGATCGCCGGTTTTTCGATCCGATTGCATTTATATCTGATAGaaata                                                                       >  1:1819331/1‑146 (MQ=255)
                                                                            gATCACGGTACGCTGGCAGAGCATGATATCTATAGTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATaaaaa                                                                       <  2:3557077/148‑1 (MQ=255)
                                                                             aTCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATaaaaaa                                                                      >  1:3127445/1‑148 (MQ=255)
                                                                              tCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATaaaaaaa                                                                     >  2:1462572/1‑148 (MQ=255)
                                                                               cACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAAc                                                                    >  2:2380042/1‑148 (MQ=255)
                                                                                aCGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAAcc                                                                   <  1:2791714/148‑1 (MQ=255)
                                                                                  ggTACGCTGGCAGAGCATGATATCTAGATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCg                                                                 <  2:2531040/148‑1 (MQ=255)
                                                                                   gTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGc                                                                >  1:3132024/1‑148 (MQ=255)
                                                                                     aCGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGccc                                                              >  2:2861316/1‑148 (MQ=255)
                                                                                        cTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTg                                                           <  2:7946/148‑1 (MQ=255)
                                                                                          ggCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGAc                                                         <  1:2243580/148‑1 (MQ=255)
                                                                                           tcAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACa                                                        >  2:2713127/2‑148 (MQ=255)
                                                                                              gagCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGc                                                     <  2:1573266/148‑1 (MQ=255)
                                                                                                 cATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCggg                                                  >  2:1489088/1‑148 (MQ=255)
                                                                                                  aTGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGga                                                 <  2:634301/148‑1 (MQ=255)
                                                                                                   tGATATCTATATTGCCGGACGTTTTGAGCTGGCGAAAATTGCCCGTGACCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaa                                                <  1:1529248/148‑1 (MQ=255)
                                                                                                   tGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaa                                                >  2:620015/1‑148 (MQ=255)
                                                                                                   tGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaa                                                >  1:3174543/1‑148 (MQ=255)
                                                                                                   tGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaa                                                >  1:1070460/1‑148 (MQ=255)
                                                                                                   tGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaa                                                >  1:1069087/1‑148 (MQ=255)
                                                                                                    gATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaag                                               >  2:2349325/1‑148 (MQ=255)
                                                                                                     atatCTCTATGGCCGGACGTTTAGAGATGGCGAAAAGTGCCCGCGATCTGTTTCGCAGTGAGCGTAATGCGCGGGACGCTCGCCTGTTTGGCGATGCGATCGCATAGATCGGAGATATACAAAAACCCGCCCCTGACAGGCGGgcaga                                              <  2:1720483/148‑1 (MQ=255)
                                                                                                     atatCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaaga                                              <  2:3482832/148‑1 (MQ=255)
                                                                                                     atatCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaaga                                              <  2:1961209/148‑1 (MQ=255)
                                                                                                      tatCTATATTGCCTGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAAACCGCCCAAGACAGGCGGgaagac                                             >  2:3261062/1‑147 (MQ=255)
                                                                                                       atCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAAc                                            <  2:39022/148‑1 (MQ=255)
                                                                                                       atCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAAc                                            <  1:2631387/148‑1 (MQ=255)
                                                                                                       atCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAAc                                            >  1:1955923/1‑148 (MQ=255)
                                                                                                       atCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAAc                                            >  1:1867857/1‑148 (MQ=255)
                                                                                                        tCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACg                                           >  1:979363/1‑148 (MQ=255)
                                                                                                         cTATATTGCCGGACGTTTTGAGATGGCGAAAATTTCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACgg                                          >  1:1649662/1‑148 (MQ=255)
                                                                                                           atatTGCCGGACGTTTTGAGATGGCGAACATTGCCCGCGATCTGTTTGGCAGTGAGCGTAATGGGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCa                                        <  2:3547651/148‑1 (MQ=255)
                                                                                                           atatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCa                                        >  1:3239421/1‑148 (MQ=255)
                                                                                                           atatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCa                                        >  1:3243537/1‑148 (MQ=255)
                                                                                                            tatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCaa                                       >  1:231994/1‑148 (MQ=255)
                                                                                                            tatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCaa                                       >  1:3343607/1‑148 (MQ=255)
                                                                                                                gCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTaa                                   <  2:1835794/148‑1 (MQ=255)
                                                                                                                 ccGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTaaa                                  <  1:1896310/148‑1 (MQ=255)
                                                                                                                    gACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTg                               <  2:1226081/148‑1 (MQ=255)
                                                                                                                      cGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGtt                             <  1:3409638/148‑1 (MQ=255)
                                                                                                                           tGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCa                        <  1:2349325/148‑1 (MQ=255)
                                                                                                                            gagaTGGCGAAAAGTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAg                       <  2:2198517/148‑1 (MQ=255)
                                                                                                                               aTGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTgg                    >  2:786694/1‑148 (MQ=255)
                                                                                                                               aTGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTgg                    >  2:2924613/1‑148 (MQ=255)
                                                                                                                                tGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGc                   <  1:1199685/148‑1 (MQ=255)
                                                                                                                                tGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGc                   <  1:2533322/148‑1 (MQ=255)
                                                                                                                                 ggCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCa                  <  1:266090/148‑1 (MQ=255)
                                                                                                                                 ggCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCa                  <  1:1332274/148‑1 (MQ=255)
                                                                                                                                  gCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAt                 <  1:3554049/148‑1 (MQ=255)
                                                                                                                                    gAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAttt               <  1:2825975/148‑1 (MQ=255)
                                                                                                                                    gAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAttt               <  2:810517/148‑1 (MQ=255)
                                                                                                                                      aacttGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAg             <  2:519338/145‑1 (MQ=255)
                                                                                                                                      aaaTTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAg             >  1:998079/1‑148 (MQ=255)
                                                                                                                                      aaaTTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAg             >  1:2736104/1‑148 (MQ=255)
                                                                                                                                        aTTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGAt           <  2:3507228/148‑1 (MQ=255)
                                                                                                                                        aTTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGAt           >  2:1158109/1‑148 (MQ=255)
                                                                                                                                          tGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCt         <  2:735629/148‑1 (MQ=255)
                                                                                                                                             ccGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATg      <  2:2051736/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGT  >  CP009273/4020375‑4020667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑