Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,046,172 A→T 100% K256N (AAA→AAT)  hemN → coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,046,1720AT97.0% 563.3 / 4.9 179K256N (AAA→AAT) hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base T (93/80);  total (96/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 181 total aligned reads displayed.

GACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGC  >  CP009273/4046029‑4046316
                                                                                                                                               |                                                                                                                                                
gACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAatgatg                                                                                                                                              <  1:3512205/148‑1 (MQ=255)
  ccTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCt                                                                                                                                            >  1:499568/1‑148 (MQ=255)
    tGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCCGCGCAAAAACAAAGATGCTGa                                                                                                                                          >  2:2225603/1‑148 (MQ=255)
    tGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGa                                                                                                                                          >  1:3306197/1‑148 (MQ=255)
    tGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGa                                                                                                                                          <  1:2068471/148‑1 (MQ=255)
    tGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGa                                                                                                                                          <  1:2880769/148‑1 (MQ=255)
     gATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAc                                                                                                                                         <  1:119593/148‑1 (MQ=255)
      aTTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAcc                                                                                                                                        <  2:3371529/148‑1 (MQ=255)
      aTTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAcc                                                                                                                                        <  2:436480/148‑1 (MQ=255)
           cGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCg                                                                                                                                   <  1:599271/148‑1 (MQ=255)
             gCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAg                                                                                                                                 >  2:2712024/1‑148 (MQ=255)
                 gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCg                                                                                                                             >  1:2766058/1‑148 (MQ=255)
                 gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCg                                                                                                                             <  1:2534700/148‑1 (MQ=255)
                 gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCg                                                                                                                             >  1:989514/1‑148 (MQ=255)
                 gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCg                                                                                                                             >  1:1899030/1‑148 (MQ=255)
                 gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCGTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCg                                                                                                                             >  1:1898720/1‑148 (MQ=255)
                     aaaCAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcagc                                                                                                                         >  1:375224/1‑148 (MQ=255)
                       aCAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaa                                                                                                                       >  1:3313565/1‑148 (MQ=255)
                        cAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaa                                                                                                                      <  1:3202197/148‑1 (MQ=255)
                        cAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaa                                                                                                                      >  2:394199/1‑148 (MQ=255)
                        cAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaa                                                                                                                      <  1:1574729/148‑1 (MQ=255)
                          gACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaaaa                                                                                                                    <  1:1382691/148‑1 (MQ=255)
                           aCGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAAc                                                                                                                   >  1:3519870/1‑148 (MQ=255)
                           aCGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAAc                                                                                                                   >  1:316004/1‑148 (MQ=255)
                              ccGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCg                                                                                                                >  1:2989167/1‑148 (MQ=255)
                                   gagTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATc                                                                                                           <  1:247296/148‑1 (MQ=255)
                                    agTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATcc                                                                                                          >  1:807329/1‑148 (MQ=255)
                                       ttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGc                                                                                                       >  1:1819490/1‑148 (MQ=255)
                                        tCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCa                                                                                                      >  1:1236286/1‑148 (MQ=255)
                                         cGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAg                                                                                                     <  2:3139729/148‑1 (MQ=255)
                                          gCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAgg                                                                                                    <  2:2051935/148‑1 (MQ=255)
                                             tttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                                                                 >  2:3264512/1‑148 (MQ=255)
                                             tttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCATCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                                                                 >  1:2853750/1‑148 (MQ=255)
                                               tACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAcc                                                                                               <  1:1624388/148‑1 (MQ=255)
                                               tACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAcc                                                                                               <  1:3154637/148‑1 (MQ=255)
                                                aCCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCa                                                                                              <  2:196389/148‑1 (MQ=255)
                                                     gAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGcc                                                                                         <  2:2202152/148‑1 (MQ=255)
                                                       aaCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCtt                                                                                       >  1:2724920/1‑148 (MQ=255)
                                                        aCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTc                                                                                      >  1:3023565/1‑148 (MQ=255)
                                                        aCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTc                                                                                      <  2:560117/148‑1 (MQ=255)
                                                         cGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTcc                                                                                     <  1:2153186/148‑1 (MQ=255)
                                                           tgtgGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                                                                   >  2:2730755/1‑148 (MQ=255)
                                                           tgtgGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                                                                   >  2:3446476/1‑148 (MQ=255)
                                                                 ggAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCaa                                                                             <  1:383127/148‑1 (MQ=255)
                                                                 ggAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCaa                                                                             <  1:3264512/148‑1 (MQ=255)
                                                                  gAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCACAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAt                                                                            >  2:1447569/1‑148 (MQ=255)
                                                                  gAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAt                                                                            <  1:3465364/148‑1 (MQ=255)
                                                                    gCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCg                                                                          >  2:1725666/1‑148 (MQ=255)
                                                                       gAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGc                                                                       >  1:860717/1‑148 (MQ=255)
                                                                         aCCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTa                                                                     >  1:1139656/1‑148 (MQ=255)
                                                                         aCCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTa                                                                     <  2:2237108/148‑1 (MQ=255)
                                                                         aCCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTa                                                                     <  2:1105861/148‑1 (MQ=255)
                                                                          ccccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAt                                                                    <  1:887443/148‑1 (MQ=255)
                                                                          ccccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAt                                                                    >  1:2942707/1‑148 (MQ=255)
                                                                           cccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATc                                                                   >  1:1446265/1‑148 (MQ=255)
                                                                           cccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATc                                                                   <  2:1874173/148‑1 (MQ=255)
                                                                           cccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATc                                                                   <  2:1061230/148‑1 (MQ=255)
                                                                             cGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCCAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAg                                                                 >  2:3192751/1‑148 (MQ=255)
                                                                             cGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCATAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTATATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAg                                                                 <  2:819893/148‑1 (MQ=255)
                                                                             cGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAg                                                                 <  2:888535/148‑1 (MQ=255)
                                                                              gATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                >  1:1228141/1‑148 (MQ=255)
                                                                              gATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                >  2:1802993/1‑148 (MQ=255)
                                                                              gATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                <  2:3116538/148‑1 (MQ=255)
                                                                                tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGttt                                                              >  1:3045989/1‑148 (MQ=255)
                                                                                tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTACCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGttt                                                              >  1:1090695/1‑148 (MQ=255)
                                                                                   tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATc                                                           >  2:118691/1‑148 (MQ=255)
                                                                                      gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                        <  2:1622756/148‑1 (MQ=255)
                                                                                      gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                        <  1:1745639/148‑1 (MQ=255)
                                                                                       aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTa                                                       >  2:3315130/1‑148 (MQ=255)
                                                                                        gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAt                                                      >  1:90198/1‑148 (MQ=255)
                                                                                        gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAt                                                      <  1:3168731/148‑1 (MQ=255)
                                                                                          gtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATgg                                                    <  2:2100011/148‑1 (MQ=255)
                                                                                              ttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCa                                                <  2:1173688/148‑1 (MQ=255)
                                                                                                aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt                                              <  2:3077456/148‑1 (MQ=255)
                                                                                                aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt                                              <  2:375224/148‑1 (MQ=255)
                                                                                                aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt                                              <  1:574744/148‑1 (MQ=255)
                                                                                                aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt                                              <  2:3307832/148‑1 (MQ=255)
                                                                                                aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt                                              <  2:131849/148‑1 (MQ=255)
                                                                                                     cgcgCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGcc                                         <  1:3012303/148‑1 (MQ=255)
                                                                                                         cATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTc                                     >  2:2333222/1‑148 (MQ=255)
                                                                                                         cATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTc                                     >  1:1010426/1‑148 (MQ=255)
                                                                                                           tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCACTCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCg                                   >  1:1335857/1‑148 (MQ=255)
                                                                                                           tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCg                                   >  1:703483/1‑148 (MQ=255)
                                                                                                            cTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCgg                                  <  2:2593795/148‑1 (MQ=255)
                                                                                                                  aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGTCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACg                            <  1:2113016/148‑1 (MQ=255)
                                                                                                                   ccATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGa                           >  1:362055/1‑148 (MQ=255)
                                                                                                                    cATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAg                          <  2:362055/148‑1 (MQ=255)
                                                                                                                    cATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCATGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAg                          <  1:977859/148‑1 (MQ=255)
                                                                                                                     attatagctgctCAGCGCAAAATCAATGATGCTGACCAGCCGAGTCCGCAGCAAAAACTCGATATCCGGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGc                         <  2:45860/142‑1 (MQ=255)
                                                                                                                     aTTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGc                         <  2:953136/148‑1 (MQ=255)
                                                                                                                        tttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTgg                      <  2:2942707/148‑1 (MQ=255)
                                                                                                                            ctgctCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGt                  <  2:1899030/148‑1 (MQ=255)
                                                                                                                               ctCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGc               <  1:2603412/148‑1 (MQ=255)
                                                                                                                                       aaaaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTg       >  1:903743/1‑148 (MQ=255)
                                                                                                                                        aaaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGa      >  2:2416095/1‑148 (MQ=255)
                                                                                                                                        aaaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGa      >  1:2040631/1‑148 (MQ=255)
                                                                                                                                        aaaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGa      >  1:107930/1‑148 (MQ=255)
                                                                                                                                        aaaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGa      >  1:107908/1‑148 (MQ=255)
                                                                                                                                          aTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAg    >  2:1753392/1‑148 (MQ=255)
                                                                                                                                            cAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGc  >  2:3480755/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGC  >  CP009273/4046029‑4046316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑