Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,058,809 G→T 100% I106I (ATC→ATA)  yihO ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,058,8090GT97.0% 585.2 / 6.6 212I106I (ATC→ATA) yihOputative transporter
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base T (94/110);  total (100/112)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

CGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGC  >  CP009273/4058664‑4058952
                                                                                                                                                 |                                                                                                                                               
cgAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTAt                                                                                                                                               >  2:57608/1‑148 (MQ=255)
  aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATaa                                                                                                                                             >  1:347317/1‑148 (MQ=255)
  aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATaa                                                                                                                                             >  1:2495799/1‑148 (MQ=255)
    cTGCGCTCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAAc                                                                                                                                           <  2:1639608/148‑1 (MQ=255)
      gcgcgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTg                                                                                                                                         <  1:299862/148‑1 (MQ=255)
            gcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGt                                                                                                                                   <  2:2786263/148‑1 (MQ=255)
            gcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGt                                                                                                                                   <  1:1839126/148‑1 (MQ=255)
             cTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGtt                                                                                                                                  <  2:279243/148‑1 (MQ=255)
                  tCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAAt                                                                                                                             <  1:1145196/148‑1 (MQ=255)
                    gggTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTa                                                                                                                           <  2:2020551/148‑1 (MQ=255)
                     ggTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTaa                                                                                                                          >  1:431512/1‑148 (MQ=255)
                     ggTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTaa                                                                                                                          >  1:2313170/1‑148 (MQ=255)
                       tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                        <  2:529844/148‑1 (MQ=255)
                       tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                        <  2:911805/148‑1 (MQ=255)
                       tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                        <  2:2432579/148‑1 (MQ=255)
                          ttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgccg                                                                                                                     >  2:1239495/1‑148 (MQ=255)
                            ggTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACgccgcc                                                                                                                   <  2:2494336/148‑1 (MQ=255)
                              tAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCgg                                                                                                                 >  2:1549723/1‑148 (MQ=255)
                               aaTTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGa                                                                                                                <  2:381414/148‑1 (MQ=255)
                                aTTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGaa                                                                                                               <  2:3097655/148‑1 (MQ=255)
                                  tGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAAcc                                                                                                             >  2:3195236/1‑148 (MQ=255)
                                    ccGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                           <  2:1842343/148‑1 (MQ=255)
                                      gggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTg                                                                                                         <  2:2129965/148‑1 (MQ=255)
                                       ggATCATCGCACCATACGAGCAGTTCATAAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTGTTAAACCGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACAGCTGc                                                                                                        >  1:2627467/1‑148 (MQ=255)
                                         atcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCat                                                                                                      <  2:2590506/148‑1 (MQ=255)
                                             tcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGACCCGCTGCCTataa                                                                                                  >  2:3508757/1‑148 (MQ=255)
                                             tcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataa                                                                                                  <  2:902243/148‑1 (MQ=255)
                                             tcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataa                                                                                                  >  2:1858859/1‑148 (MQ=255)
                                              cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                                                                                 <  2:921231/148‑1 (MQ=255)
                                              cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                                                                                 <  1:1046227/148‑1 (MQ=255)
                                               gCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaa                                                                                                <  1:1071751/148‑1 (MQ=255)
                                                   cATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataaa                                                                                            <  1:518655/148‑1 (MQ=255)
                                                   cATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataaa                                                                                            >  1:761441/1‑148 (MQ=255)
                                                      aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                         <  2:2890732/148‑1 (MQ=255)
                                                      aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                         <  1:2271075/148‑1 (MQ=255)
                                                      aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                         >  2:1786839/1‑148 (MQ=255)
                                                       cGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGa                                                                                        <  2:505646/148‑1 (MQ=255)
                                                         aGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACg                                                                                      >  1:328469/1‑148 (MQ=255)
                                                         aGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACg                                                                                      >  1:252890/1‑148 (MQ=255)
                                                         aGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACg                                                                                      >  2:2973129/1‑148 (MQ=255)
                                                          gCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGa                                                                                     <  2:3411740/148‑1 (MQ=255)
                                                             gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAATGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAt                                                                                  >  1:791877/1‑148 (MQ=255)
                                                              ttCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAtt                                                                                 >  2:131911/1‑148 (MQ=255)
                                                               tcatcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAttt                                                                                <  2:3316923/148‑1 (MQ=255)
                                                                catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTg                                                                               <  2:696514/148‑1 (MQ=255)
                                                                   caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTTGTGGCGATAAACTGGAGCGGTGCCATTAACGCCGCCGGAACCGCAGCATAAAAAAAAAAGGGACGAAATTTTCCt                                                                            >  2:602928/1‑148 (MQ=255)
                                                                   caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCt                                                                            <  1:1412141/148‑1 (MQ=255)
                                                                    aGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCtt                                                                           >  2:200116/1‑148 (MQ=255)
                                                                    aGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCtt                                                                           >  2:1294773/1‑148 (MQ=255)
                                                                    aGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCtt                                                                           <  2:568999/148‑1 (MQ=255)
                                                                     gACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCttt                                                                          <  2:2914160/148‑1 (MQ=255)
                                                                          tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGcc                                                                     <  2:1059728/148‑1 (MQ=255)
                                                                          tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGcc                                                                     <  2:1911422/148‑1 (MQ=255)
                                                                               aaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAata                                                                <  2:56359/148‑1 (MQ=255)
                                                                                 gTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                              <  2:796874/148‑1 (MQ=255)
                                                                                  tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttt                                                             >  2:404112/1‑148 (MQ=255)
                                                                                  tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttt                                                             <  1:3382198/148‑1 (MQ=255)
                                                                                   ccAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtttt                                                            >  1:3152055/1‑148 (MQ=255)
                                                                                     aaaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTc                                                          <  2:3007797/148‑1 (MQ=255)
                                                                                     aaaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTc                                                          <  2:1251867/148‑1 (MQ=255)
                                                                                     aaaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTc                                                          <  2:88766/148‑1 (MQ=255)
                                                                                     aaaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTc                                                          <  2:2495799/148‑1 (MQ=255)
                                                                                      aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTcc                                                         >  2:2772641/1‑148 (MQ=255)
                                                                                      aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTcc                                                         >  2:3127990/1‑148 (MQ=255)
                                                                                      aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTcc                                                         >  2:781142/1‑148 (MQ=255)
                                                                                         atcatAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCcgcg                                                      >  1:1787923/1‑148 (MQ=255)
                                                                                                aCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                               <  2:861851/148‑1 (MQ=255)
                                                                                                 cAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATTAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTTCCTTTAGGCCCAATATTTTTGCGcgaggcgagt                                              >  1:2710183/1‑148 (MQ=255)
                                                                                                    cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaaa                                           <  2:1349127/148‑1 (MQ=255)
                                                                                                    cgcgGTGGCAATCGTCGTTTTAAACGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAAGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAATTGCCTTTAGGCCCAATATTTTTACGCGAGTCGAGTaaa                                           >  1:690920/1‑148 (MQ=255)
                                                                                                    cgcgGTGGCAATCGTCGTGTTAACCGGCAAACAAAAGGTGGTTGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAATGGACGAAATTTGCCTTTAGGCCCAATATTTTTACGCGAGTCGAGTaaa                                           >  1:691039/1‑148 (MQ=255)
                                                                                                           gCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCgg                                    <  2:1917756/148‑1 (MQ=255)
                                                                                                            cAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGt                                   <  1:3195236/148‑1 (MQ=255)
                                                                                                            cAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGt                                   >  2:113031/1‑148 (MQ=255)
                                                                                                                cgtcgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGc                               <  1:2338911/148‑1 (MQ=255)
                                                                                                                cgtcgtTTTAACCCGCAAAAAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCCCCCGAACCGCTGCAAATAAAATAAATGGACGAAAATTTCCATTAGGCCCAAAATTTTTACGCGAGTAGAGTAAAAAAACGGTGAGc                               >  2:3224846/1‑148 (MQ=255)
                                                                                                                  tcgtTTTAACCGGCAAACAATAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCat                             <  1:486734/148‑1 (MQ=255)
                                                                                                                  tcgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCat                             <  1:630821/148‑1 (MQ=255)
                                                                                                                   cgtTTTAACCGGCAAAGAAAAGGTGGTGGCTATTAAGTGGAGCGTTGCTATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCatt                            <  2:2627467/148‑2 (MQ=255)
                                                                                                                   cgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCata                            >  2:3213212/1‑148 (MQ=255)
                                                                                                                     ttttAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATc                          >  1:2915643/1‑148 (MQ=255)
                                                                                                                      tttAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                         <  1:131911/148‑1 (MQ=255)
                                                                                                                           ccGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTaaa                    >  2:106888/1‑148 (MQ=255)
                                                                                                                           ccGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTaaa                    >  1:3371889/1‑148 (MQ=255)
                                                                                                                           ccGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTaaa                    >  2:107330/1‑148 (MQ=255)
                                                                                                                             ggCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAcg                  <  2:2312766/148‑1 (MQ=255)
                                                                                                                                  acaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTa             >  1:1269583/1‑148 (MQ=255)
                                                                                                                                    aaaaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaa           >  1:3052751/1‑148 (MQ=255)
                                                                                                                                    aaaaGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaa           >  1:354746/1‑148 (MQ=255)
                                                                                                                                     aaaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaa          >  1:160172/1‑148 (MQ=255)
                                                                                                                                     aaaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaa          >  1:2165782/1‑148 (MQ=255)
                                                                                                                                      taGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGATGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa         <  2:1878754/147‑1 (MQ=255)
                                                                                                                                      aaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa         >  2:1472233/1‑148 (MQ=255)
                                                                                                                                        ggtggtGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAAc       <  1:2777362/148‑1 (MQ=255)
                                                                                                                                         gtggtgGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACt      <  2:3400953/148‑1 (MQ=255)
                                                                                                                                          tggtggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACtt     <  2:2971112/148‑1 (MQ=255)
                                                                                                                                          tggtggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACtt     <  1:288371/148‑1 (MQ=255)
                                                                                                                                            gtggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCg   >  1:3261117/1‑148 (MQ=255)
                                                                                                                                            gtggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCg   >  2:2084826/1‑148 (MQ=255)
                                                                                                                                             tggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGc  <  1:2046356/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGC  >  CP009273/4058664‑4058952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑