Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,124,384 C→A 100% H208N (CAT→AAT)  katG → catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,124,3840CA98.2% 522.9 / 0.9 170H208N (CAT→AAT) katGcatalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (87/80);  total (88/82)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 170 total aligned reads displayed.

GGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGAAC  >  CP009273/4124239‑4124527
                                                                                                                                                 |                                                                                                                                               
ggCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAAt                                                                                                                                               >  1:2155285/1‑148 (MQ=255)
    gACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCgg                                                                                                                                           <  2:875573/148‑1 (MQ=255)
     aCCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGa                                                                                                                                          >  1:1837895/1‑148 (MQ=255)
      ccTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCGTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGaa                                                                                                                                         <  2:1170166/148‑1 (MQ=255)
       cTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAg                                                                                                                                        <  1:3160551/148‑1 (MQ=255)
         gTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAgcg                                                                                                                                      >  1:394175/1‑148 (MQ=255)
         gTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAgcg                                                                                                                                      >  1:2372220/1‑148 (MQ=255)
          tttATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGGTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAgcgc                                                                                                                                     >  1:3334396/1‑148 (MQ=255)
              tCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGc                                                                                                                                 >  1:2824727/1‑148 (MQ=255)
               ccTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCg                                                                                                                                >  2:2653270/1‑148 (MQ=255)
               ccTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCg                                                                                                                                >  2:2654281/1‑148 (MQ=255)
                  cgcgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGaaa                                                                                                                             <  2:1380698/148‑1 (MQ=255)
                   gcgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAg                                                                                                                            <  1:2023360/148‑1 (MQ=255)
                    cgGGTCACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTGTTGGTGCCGGTCGTGAAGACGTCTGGGACCCGGATCTGGATGTGAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTACTCCGGACGCGCTGGCGAAAGc                                                                                                                           >  2:3042223/1‑148 (MQ=255)
                    cgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGACGCGCTGGCGAAAGc                                                                                                                           >  2:3042177/1‑148 (MQ=255)
                    cgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGc                                                                                                                           >  2:1266125/1‑148 (MQ=255)
                     gggTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCa                                                                                                                          <  1:1305325/148‑1 (MQ=255)
                        tAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCg                                                                                                                       >  1:2620170/1‑148 (MQ=255)
                           cGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTg                                                                                                                    >  2:627931/1‑148 (MQ=255)
                           cGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTg                                                                                                                    <  1:1639632/148‑1 (MQ=255)
                                     gAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCg                                                                                                          >  2:1911172/1‑148 (MQ=255)
                                        aaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCgaga                                                                                                       <  1:3465849/148‑1 (MQ=255)
                                        aaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCgaga                                                                                                       <  1:2679512/148‑1 (MQ=255)
                                         aCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGAt                                                                                                      <  2:1847806/148‑1 (MQ=255)
                                         aCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGAt                                                                                                      <  2:2316513/148‑1 (MQ=255)
                                         aCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGAt                                                                                                      <  2:1229708/148‑1 (MQ=255)
                                          cTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATg                                                                                                     >  2:163758/1‑148 (MQ=255)
                                            ccGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATggg                                                                                                   >  2:1625598/1‑148 (MQ=255)
                                               gCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCt                                                                                                <  2:690280/148‑1 (MQ=255)
                                               gCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCt                                                                                                >  1:3253873/1‑148 (MQ=255)
                                               gCTTCCGTAACTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCt                                                                                                <  1:2608020/148‑1 (MQ=255)
                                                 ttCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGa                                                                                              >  1:3535869/1‑148 (MQ=255)
                                                   ccGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAtt                                                                                            <  2:1607364/148‑1 (MQ=255)
                                                   ccGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAtt                                                                                            <  2:3374437/148‑1 (MQ=255)
                                                   ccGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAtt                                                                                            <  2:2867166/148‑1 (MQ=255)
                                                       aCCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACg                                                                                        >  1:1246603/1‑148 (MQ=255)
                                                        ccTTCGGTTTTGGTGCCGGTCGTGAAGACGTATGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGt                                                                                       <  1:2451794/148‑1 (MQ=255)
                                                         cTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGtt                                                                                      >  1:1114606/1‑148 (MQ=255)
                                                         cTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGtt                                                                                      <  1:2666805/148‑1 (MQ=255)
                                                          ttCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTa                                                                                     <  1:625792/148‑1 (MQ=255)
                                                            cGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAAc                                                                                   <  1:941937/148‑1 (MQ=255)
                                                             ggTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAAcc                                                                                  <  2:673020/148‑1 (MQ=255)
                                                               ttttGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                                                                                >  2:1016559/1‑148 (MQ=255)
                                                               ttttGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                                                                                >  2:3503217/1‑148 (MQ=255)
                                                                  ttgTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGaa                                                                             <  1:3062109/146‑1 (MQ=255)
                                                                     tGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGc                                                                          <  2:1837895/148‑1 (MQ=255)
                                                                          gTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGa                                                                     <  2:2300589/148‑1 (MQ=255)
                                                                           tCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGAt                                                                    >  2:158326/1‑148 (MQ=255)
                                                                           tCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGAt                                                                    >  2:158325/1‑148 (MQ=255)
                                                                              tGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATcac                                                                 <  2:709183/148‑1 (MQ=255)
                                                                               gAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATcaca                                                                >  2:102293/1‑148 (MQ=255)
                                                                               gAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATcaca                                                                >  2:45706/1‑148 (MQ=255)
                                                                                 aGACGTCTGGTTACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTATCCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGTTTTACGTTAACCCGGAAGGCCCGTATcacatc                                                              <  2:1670508/148‑3 (MQ=255)
                                                                                 aGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgc                                                              >  1:83692/1‑148 (MQ=255)
                                                                                 aGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgc                                                              >  1:1743023/1‑148 (MQ=255)
                                                                                 aGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgc                                                              >  1:1743025/1‑148 (MQ=255)
                                                                                 aGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgc                                                              <  2:2083292/148‑1 (MQ=255)
                                                                                  gACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcg                                                             >  1:3295484/1‑148 (MQ=255)
                                                                                        tGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAAc                                                       <  2:2429635/148‑1 (MQ=255)
                                                                                        tGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAAc                                                       <  2:2429617/148‑1 (MQ=255)
                                                                                          ggAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCg                                                     >  2:950794/1‑148 (MQ=255)
                                                                                          ggAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCg                                                     >  2:649122/1‑148 (MQ=255)
                                                                                           gAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGc                                                    >  2:2734616/1‑148 (MQ=255)
                                                                                            aaCCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCt                                                   <  1:2907074/148‑1 (MQ=255)
                                                                                             aCCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCtt                                                  >  2:583698/1‑148 (MQ=255)
                                                                                             aCCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCtt                                                  <  2:2372220/148‑1 (MQ=255)
                                                                                              ccGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCttt                                                 <  1:3275787/148‑1 (MQ=255)
                                                                                               cGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTc                                                <  2:3295484/148‑1 (MQ=255)
                                                                                               cGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTc                                                <  1:1514822/148‑1 (MQ=255)
                                                                                                   tCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCg                                            >  1:1845449/1‑148 (MQ=255)
                                                                                                    cTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCgg                                           >  1:3161753/1‑148 (MQ=255)
                                                                                                    cTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCgg                                           >  1:3161594/1‑148 (MQ=255)
                                                                                                      ggATTTTAACTGGGGTGATTAAAAAGCCTGGCTGAATCACAGTAATCCGGAATCGCTGGCGAAATCACCGCTGGGTGCAACCGAGATGGGTCTTATTTAAGTTAACACGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGgca                                         >  2:1639650/1‑148 (MQ=255)
                                                                                                       gATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGgcag                                        <  2:2725880/148‑1 (MQ=255)
                                                                                                           ttAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCagcagc                                    <  1:3298465/148‑1 (MQ=255)
                                                                                                            tAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCt                                   >  2:2856246/1‑148 (MQ=255)
                                                                                                              aCTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTAt                                 <  1:2087931/148‑1 (MQ=255)
                                                                                                              aCTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTAt                                 <  1:102293/148‑1 (MQ=255)
                                                                                                              aCTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTAt                                 >  2:2727674/1‑148 (MQ=255)
                                                                                                              aCTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTAt                                 <  1:45706/148‑1 (MQ=255)
                                                                                                               cTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATc                                <  1:2101836/148‑1 (MQ=255)
                                                                                                                       atgaAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGAc                        >  1:125706/1‑148 (MQ=255)
                                                                                                                         gaAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCt                      <  2:1069992/148‑1 (MQ=255)
                                                                                                                          aaaaaGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCtt                     <  2:3411634/148‑1 (MQ=255)
                                                                                                                            aaaGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCg                   >  1:1770344/1‑148 (MQ=255)
                                                                                                                             aaGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGAGCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCgg                  >  1:2454320/1‑148 (MQ=255)
                                                                                                                              aGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGc                 >  1:1623804/1‑148 (MQ=255)
                                                                                                                                ccTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCaa               <  2:394175/148‑1 (MQ=255)
                                                                                                                                ccTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGAACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCaa               >  1:3359765/1‑148 (MQ=255)
                                                                                                                                   ggCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACAt            >  1:975235/1‑148 (MQ=255)
                                                                                                                                   ggCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACAt            <  2:2975050/148‑1 (MQ=255)
                                                                                                                                    gCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATg           >  1:434397/1‑148 (MQ=255)
                                                                                                                                     cTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATgg          <  2:2396214/148‑1 (MQ=255)
                                                                                                                                       gACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGc        <  2:1946830/148‑1 (MQ=255)
                                                                                                                                        aCTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCa       <  2:3531153/148‑1 (MQ=255)
                                                                                                                                          tCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATg     <  2:2155285/148‑1 (MQ=255)
                                                                                                                                           cACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGa    <  1:1542600/148‑1 (MQ=255)
                                                                                                                                            aCCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGaa   <  1:649122/148‑1 (MQ=255)
                                                                                                                                            aCCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGaa   <  1:950794/148‑1 (MQ=255)
                                                                                                                                             ccGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGAac  >  1:1685560/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGAAC  >  CP009273/4124239‑4124527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑