Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,184,471 A→T 64.5% I519I (ATT→ATA)  thiC ← phosphomethylpyrimidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,184,4710AT64.5% 217.5 / 196.9 188I519I (ATT→ATA) thiCphosphomethylpyrimidine synthase
Reads supporting (aligned to +/- strand):  ref base A (41/26);  new base T (51/70);  total (92/96)
Fisher's exact test for biased strand distribution p-value = 1.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

ATCGTGATAAGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCC  >  CP009273/4184327‑4184615
                                                                                                                                                |                                                                                                                                                
aTCGTGATAAGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATc                                                                                                                                               >  1:614255/1‑148 (MQ=255)
  cGTGATAAGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGACTTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCtt                                                                                                                                             >  2:1304981/1‑148 (MQ=255)
   gTGATAAGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTta                                                                                                                                            <  1:474449/148‑1 (MQ=255)
     gATAAGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTtata                                                                                                                                          >  1:1368857/1‑148 (MQ=255)
       tAAGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAgg                                                                                                                                        <  2:2354144/148‑1 (MQ=255)
        aaGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTCTCTTATAGGt                                                                                                                                       >  2:1999105/1‑148 (MQ=255)
                 cggTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGccc                                                                                                                              >  1:2459949/1‑148 (MQ=255)
                 cggTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGccc                                                                                                                              <  2:3086891/148‑1 (MQ=255)
                  ggTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGcccc                                                                                                                             <  2:2198897/148‑1 (MQ=255)
                  ggTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGcccc                                                                                                                             <  1:2061305/148‑1 (MQ=255)
                     aaaCGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGc                                                                                                                          <  2:486621/148‑1 (MQ=255)
                      aaCGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCt                                                                                                                         >  2:1703338/1‑148 (MQ=255)
                          ggTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAAc                                                                                                                     >  2:689847/1‑148 (MQ=255)
                            tCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACAt                                                                                                                   <  1:2242481/148‑1 (MQ=255)
                               aGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACAtctt                                                                                                                >  1:40402/1‑148 (MQ=255)
                               aGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACAtctt                                                                                                                <  2:1744604/148‑1 (MQ=255)
                                gggCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACAtcttc                                                                                                               <  2:2586522/148‑1 (MQ=255)
                                gggCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACAtcttc                                                                                                               <  2:2586497/148‑1 (MQ=255)
                                 ggCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACAtcttct                                                                                                              >  1:1402965/1‑148 (MQ=255)
                                 ggCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACAtcttct                                                                                                              <  1:492595/148‑1 (MQ=255)
                                    cAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTa                                                                                                           >  1:127192/1‑148 (MQ=255)
                                    cAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTa                                                                                                           >  1:2709357/1‑148 (MQ=255)
                                     aGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTAt                                                                                                          <  1:3272720/148‑1 (MQ=255)
                                       aTTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTg                                                                                                        >  1:1523543/1‑148 (MQ=255)
                                        ttAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTgg                                                                                                       >  1:505093/1‑148 (MQ=255)
                                        ttAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTgg                                                                                                       >  1:1095082/1‑148 (MQ=255)
                                         tAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTggg                                                                                                      >  1:1137582/1‑148 (MQ=255)
                                           aaCTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCa                                                                                                    >  1:1655683/1‑148 (MQ=255)
                                            aCTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAg                                                                                                   >  2:2267378/1‑148 (MQ=255)
                                             cTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGa                                                                                                  >  1:1579900/1‑148 (MQ=255)
                                             cTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGa                                                                                                  <  1:1104135/148‑1 (MQ=255)
                                             cTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGa                                                                                                  >  2:43595/1‑148 (MQ=255)
                                               gctCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGAcc                                                                                                <  2:3399729/146‑1 (MQ=255)
                                                gTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGAccc                                                                                               >  2:2823364/1‑148 (MQ=255)
                                                  cTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAg                                                                                             >  2:1906486/1‑148 (MQ=255)
                                                  cTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAg                                                                                             <  1:2687295/148‑1 (MQ=255)
                                                    tCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGAt                                                                                           <  2:130692/148‑1 (MQ=255)
                                                    tCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGAt                                                                                           <  2:2544337/148‑1 (MQ=255)
                                                     cccAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATg                                                                                          <  1:792577/148‑1 (MQ=255)
                                                      ccAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGc                                                                                         <  1:3127168/148‑1 (MQ=255)
                                                        aGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGctc                                                                                       <  1:2800697/148‑1 (MQ=255)
                                                            aaaTTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCtttt                                                                                   >  2:3425679/1‑148 (MQ=255)
                                                            aaaTTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCtttt                                                                                   <  2:3011088/148‑1 (MQ=255)
                                                                tCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCg                                                                               >  2:2593620/1‑148 (MQ=255)
                                                                 cGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGt                                                                              <  2:2084046/148‑1 (MQ=255)
                                                                 cGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGt                                                                              <  2:1432507/148‑1 (MQ=255)
                                                                  gAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGtt                                                                             <  2:274991/148‑1 (MQ=255)
                                                                   aaGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTa                                                                            <  2:1528048/148‑1 (MQ=255)
                                                                    aGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTAc                                                                           >  2:402163/1‑148 (MQ=255)
                                                                     gCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACg                                                                          >  1:2087434/1‑148 (MQ=255)
                                                                      cGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGt                                                                         <  2:1887567/148‑1 (MQ=255)
                                                                      cGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGt                                                                         >  2:2182675/1‑148 (MQ=255)
                                                                        ggCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTaa                                                                       >  1:2684680/1‑148 (MQ=255)
                                                                         gCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAAc                                                                      <  1:2267378/148‑1 (MQ=255)
                                                                            ttCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACaga                                                                   >  1:95895/1‑148 (MQ=255)
                                                                            ttCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACaga                                                                   <  2:3176171/148‑1 (MQ=255)
                                                                             tCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACagag                                                                  <  2:2555289/148‑1 (MQ=255)
                                                                              cGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGc                                                                 <  1:1982540/148‑1 (MQ=255)
                                                                                 cATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATc                                                              <  1:58238/148‑1 (MQ=255)
                                                                                       gTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAg                                                        >  2:2931101/1‑148 (MQ=255)
                                                                                        ttATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGc                                                       >  1:1910015/1‑148 (MQ=255)
                                                                                         tATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGcc                                                      >  1:1661382/1‑148 (MQ=255)
                                                                                         tATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGcc                                                      >  1:1736196/1‑148 (MQ=255)
                                                                                           tCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCaa                                                    >  1:489961/1‑148 (MQ=255)
                                                                                           tCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCaa                                                    >  1:56855/1‑148 (MQ=255)
                                                                                            cgcgAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCaaa                                                   >  1:1002514/1‑148 (MQ=255)
                                                                                            cgcgAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCaaa                                                   >  1:1002509/1‑148 (MQ=255)
                                                                                              cgAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAAcc                                                 <  2:1028494/148‑1 (MQ=255)
                                                                                                 aTTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGc                                              <  2:633523/148‑1 (MQ=255)
                                                                                                  tttGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCATCCAAACCAGcc                                             >  1:306742/1‑148 (MQ=255)
                                                                                                   ttGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCa                                            <  2:1089717/148‑1 (MQ=255)
                                                                                                       gcgcCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAatca                                        <  2:1553168/148‑1 (MQ=255)
                                                                                                        cgcCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAatcat                                       <  2:2350233/148‑1 (MQ=255)
                                                                                                        cgcCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAatcat                                       >  1:2199577/1‑148 (MQ=255)
                                                                                                          cccGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATcg                                     >  1:2477152/1‑148 (MQ=255)
                                                                                                           ccGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATcgc                                    <  1:2221574/148‑1 (MQ=255)
                                                                                                               aTGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCa                                <  2:167421/148‑1 (MQ=255)
                                                                                                               aTGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCa                                >  1:2258848/1‑148 (MQ=255)
                                                                                                                  cccTTTCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCa                             <  2:3278735/148‑1 (MQ=255)
                                                                                                                    cTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTGTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAAt                           >  2:2332863/1‑148 (MQ=255)
                                                                                                                     tttCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATc                          >  1:3548115/1‑148 (MQ=255)
                                                                                                                      ttCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATcc                         >  1:2441827/1‑148 (MQ=255)
                                                                                                                      ttCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATcc                         <  1:772508/148‑1 (MQ=255)
                                                                                                                      ttCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATcc                         <  2:1490538/148‑1 (MQ=255)
                                                                                                                       tCGCCAGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATccc                        <  1:2704593/148‑1 (MQ=255)
                                                                                                                            aGGTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACg                   >  1:937331/1‑148 (MQ=255)
                                                                                                                             ggTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGt                  >  2:1314891/1‑148 (MQ=255)
                                                                                                                              gTCAGCGGCGTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTg                 <  2:1402965/148‑1 (MQ=255)
                                                                                                                                  gcggcgTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGt             <  1:2978790/148‑1 (MQ=255)
                                                                                                                                  gcggcgTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGt             <  2:473158/148‑1 (MQ=255)
                                                                                                                                   cggcgTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGGGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTg            >  2:976681/1‑148 (MQ=255)
                                                                                                                                   cggcgTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTg            <  2:1137582/148‑1 (MQ=255)
                                                                                                                                   cggcgTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTg            >  1:857155/1‑148 (MQ=255)
                                                                                                                                    ggcgTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTgg           <  1:2390355/148‑1 (MQ=255)
                                                                                                                                     gcgTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGt          <  1:182658/148‑1 (MQ=255)
                                                                                                                                     gcgTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGt          >  2:569010/1‑148 (MQ=255)
                                                                                                                                      cgTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTc         >  2:1203164/1‑148 (MQ=255)
                                                                                                                                      cgTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTc         <  1:934195/148‑1 (MQ=255)
                                                                                                                                       gTGGGCAGCTATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCa        <  1:2303420/148‑1 (MQ=255)
                                                                                                                                            cagctATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGcc   >  1:2878180/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATCGTGATAAGCGCGGGCGGTAAACGGGTCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCC  >  CP009273/4184327‑4184615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑