Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,219,790 A→T 61.9% L94Q (CTG→CAG)  pepE ← (alpha)‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,219,7900AT61.9% 191.4 / 256.1 215L94Q (CTG→CAG) pepE(alpha)‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (42/38);  new base T (58/75);  total (101/114)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 215 total aligned reads displayed.

TGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCA  >  CP009273/4219646‑4219935
                                                                                                                                                |                                                                                                                                                 
 gCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCaa                                                                                                                                               <  2:1376261/148‑1 (MQ=255)
   ggATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACt                                                                                                                                             <  2:2232315/148‑1 (MQ=255)
    gATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTg                                                                                                                                            >  2:427845/1‑148 (MQ=255)
      tCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGa                                                                                                                                          >  1:3171355/1‑148 (MQ=255)
      tCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGa                                                                                                                                          >  2:2161342/1‑148 (MQ=255)
        gACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGaaa                                                                                                                                        <  2:1875880/148‑1 (MQ=255)
          cAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGt                                                                                                                                      <  1:2327507/148‑1 (MQ=255)
           aaTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTa                                                                                                                                     <  1:2398154/148‑1 (MQ=255)
           aaTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTa                                                                                                                                     <  1:569033/148‑1 (MQ=255)
            aTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAt                                                                                                                                    >  2:557200/1‑148 (MQ=255)
            aTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAt                                                                                                                                    >  2:2447069/1‑148 (MQ=255)
            aTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTAt                                                                                                                                    <  2:3079089/148‑1 (MQ=255)
             tCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAtt                                                                                                                                   <  1:2111569/148‑1 (MQ=255)
             tCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTAtt                                                                                                                                   >  2:2523619/1‑148 (MQ=255)
              cGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTc                                                                                                                                  <  1:1390509/148‑1 (MQ=255)
              cGGCATATCGTGGGTGGTACGAAGAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTc                                                                                                                                  <  1:2025426/148‑1 (MQ=255)
               ggCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTcc                                                                                                                                 <  1:1712090/148‑1 (MQ=255)
                 cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccg                                                                                                                               >  2:724742/1‑148 (MQ=255)
                   tatCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgcc                                                                                                                             >  1:3072113/1‑148 (MQ=255)
                    atCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgccg                                                                                                                            >  1:3476979/1‑148 (MQ=255)
                      cGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgccgcc                                                                                                                          >  2:2247186/1‑148 (MQ=255)
                      cGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgccgcc                                                                                                                          >  2:2354359/1‑148 (MQ=255)
                            tggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAAt                                                                                                                    <  2:2028240/148‑1 (MQ=255)
                             ggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATc                                                                                                                   <  1:2386551/148‑1 (MQ=255)
                             ggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATc                                                                                                                   <  1:1150111/148‑1 (MQ=255)
                                  gAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAt                                                                                                              <  1:2079961/148‑1 (MQ=255)
                                   aaTAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAtc                                                                                                             >  1:1432240/1‑148 (MQ=255)
                                     tAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAtctc                                                                                                           >  1:658347/1‑148 (MQ=255)
                                        ttGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGc                                                                                                        >  1:1713323/1‑148 (MQ=255)
                                        ttGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGc                                                                                                        >  2:2087970/1‑148 (MQ=255)
                                          gggCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAt                                                                                                      <  2:27440/148‑1 (MQ=255)
                                          gggCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAt                                                                                                      <  2:3210726/148‑1 (MQ=255)
                                          gggCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAt                                                                                                      <  1:428937/148‑1 (MQ=255)
                                           ggCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAtt                                                                                                     <  2:2534566/148‑1 (MQ=255)
                                           ggCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAtt                                                                                                     <  2:628723/148‑1 (MQ=255)
                                              aagcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTc                                                                                                  <  2:1700237/148‑1 (MQ=255)
                                               agcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCa                                                                                                 >  2:1219737/1‑148 (MQ=255)
                                               agcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCa                                                                                                 >  1:829328/1‑148 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                >  1:3088427/1‑148 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                <  2:955199/148‑1 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                >  1:3088390/1‑148 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                >  2:3105773/1‑148 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                <  2:1442309/148‑1 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                <  2:3422907/148‑1 (MQ=255)
                                                 caagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAAt                                                                                               <  1:1938427/148‑1 (MQ=255)
                                                    ggTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATcgc                                                                                            >  2:905169/1‑148 (MQ=255)
                                                    ggTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATcgc                                                                                            >  2:2690150/1‑148 (MQ=255)
                                                    ggTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATcgc                                                                                            >  2:1946153/1‑148 (MQ=255)
                                                      ttAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCgg                                                                                          >  1:346196/1‑148 (MQ=255)
                                                      ttAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCgg                                                                                          <  1:1011845/148‑1 (MQ=255)
                                                       tAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGc                                                                                         >  1:523062/1‑148 (MQ=255)
                                                        aGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCa                                                                                        <  2:1242043/148‑1 (MQ=255)
                                                             cTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACggg                                                                                   >  1:2154497/1‑148 (MQ=255)
                                                                cgcTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATc                                                                                <  1:2247186/148‑1 (MQ=255)
                                                                  cTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGa                                                                              <  2:3361884/148‑1 (MQ=255)
                                                                     cAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCGACaa                                                                           <  1:1595362/148‑1 (MQ=255)
                                                                     cAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCGACaa                                                                           <  1:3360130/148‑1 (MQ=255)
                                                                        ccAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACCATCACGATCTCAGCATTTTCAATCGCGGCAACGGGCTCTACAACGc                                                                        >  2:826158/1‑148 (MQ=255)
                                                                        ccAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGc                                                                        >  1:706511/1‑148 (MQ=255)
                                                                        ccAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGc                                                                        >  2:205121/1‑148 (MQ=255)
                                                                         cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCGACAACGCt                                                                       <  1:458369/148‑1 (MQ=255)
                                                                         cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCt                                                                       >  2:2150161/1‑148 (MQ=255)
                                                                              taCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCAGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGaa                                                                  >  2:2780919/1‑148 (MQ=255)
                                                                                cAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATa                                                                <  2:3557089/148‑1 (MQ=255)
                                                                                 agagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATAc                                                               <  2:338947/148‑1 (MQ=255)
                                                                                          cGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAg                                                      >  2:3051470/1‑148 (MQ=255)
                                                                                           gTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGa                                                     <  1:1589787/148‑1 (MQ=255)
                                                                                              tAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAac                                                  <  2:1932765/148‑1 (MQ=255)
                                                                                              tAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACAGGATCTACAACGCTATGAATACCGGTGACAGAAac                                                  <  2:131073/148‑1 (MQ=255)
                                                                                               aaCCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAaca                                                 <  1:454227/148‑1 (MQ=255)
                                                                                                 ccACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACAcc                                               >  1:2211248/1‑148 (MQ=255)
                                                                                                  cACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACAccc                                              >  1:2541943/1‑148 (MQ=255)
                                                                                                    cGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCATTCGCGGAAACGGGATCGACAACGCTATGAATACCGGTGACAGAATCACCCAg                                            <  2:3542162/148‑1 (MQ=255)
                                                                                                    cGTCAGTAATTGGTGCCAGAAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAg                                            <  1:2621184/148‑1 (MQ=255)
                                                                                                       cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                         <  2:1902884/148‑1 (MQ=255)
                                                                                                       cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                         >  2:1590938/1‑148 (MQ=255)
                                                                                                          tAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAATGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGc                                      >  1:2064966/1‑148 (MQ=255)
                                                                                                           aaTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                     <  2:3086578/148‑1 (MQ=255)
                                                                                                           aaTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                     <  1:907213/148‑1 (MQ=255)
                                                                                                            aTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCga                                    >  2:971916/1‑148 (MQ=255)
                                                                                                            aTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCga                                    <  1:61687/148‑1 (MQ=255)
                                                                                                            aTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCga                                    <  2:2674618/148‑1 (MQ=255)
                                                                                                               ggTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGaa                                 <  1:2087970/148‑1 (MQ=255)
                                                                                                                  gCCAGCAGCCCGCACTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCg                              <  1:3166371/148‑1 (MQ=255)
                                                                                                                            cgcgCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtttt                    <  1:2416447/148‑1 (MQ=255)
                                                                                                                                 tCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtg               <  1:35627/148‑1 (MQ=255)
                                                                                                                                 tCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtg               <  1:1923164/148‑1 (MQ=255)
                                                                                                                                 tCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtg               <  1:1840104/148‑1 (MQ=255)
                                                                                                                                      gCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTaa          <  2:350190/148‑1 (MQ=255)
                                                                                                                                      gCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTaa          >  1:1360865/1‑148 (MQ=255)
                                                                                                                                      gCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTaa          >  1:856965/1‑148 (MQ=255)
                                                                                                                                       cACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAat         >  1:803248/1‑148 (MQ=255)
                                                                                                                                       cACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAat         >  1:2738423/1‑148 (MQ=255)
                                                                                                                                        aCTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc        <  2:1556726/148‑1 (MQ=255)
                                                                                                                                        aCTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc        <  2:706649/148‑1 (MQ=255)
                                                                                                                                          tGTTTCAGCAACTGTAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcat      >  1:601075/1‑148 (MQ=255)
                                                                                                                                           gTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcatc     <  1:2365506/148‑1 (MQ=255)
                                                                                                                                           gTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACTGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcatc     <  2:2381500/148‑1 (MQ=255)
                                                                                                                                           gTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcatc     >  2:150332/1‑148 (MQ=255)
                                                                                                                                            tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc    >  1:1158346/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCA  >  CP009273/4219646‑4219935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑