Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,802 C→A 100% I662I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,8020CA96.8% 535.1 / 4.4 194I662I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/9);  new base A (90/95);  total (90/104)
Fisher's exact test for biased strand distribution p-value = 3.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 194 total aligned reads displayed.

ACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCT  >  CP009273/32657‑32944
                                                                                                                                                 |                                                                                                                                              
acagCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                              <  1:3476542/145‑1 (MQ=255)
        tACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGcc                                                                                                                                      >  1:190295/1‑148 (MQ=255)
          cTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCg                                                                                                                                    >  1:2241165/1‑148 (MQ=255)
            tCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGa                                                                                                                                  >  1:1002187/1‑148 (MQ=255)
             cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGAt                                                                                                                                 <  1:2066031/148‑1 (MQ=255)
                 ccGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTa                                                                                                                             <  1:180479/148‑1 (MQ=255)
                    gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCg                                                                                                                          <  1:706134/148‑1 (MQ=255)
                    gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCg                                                                                                                          >  2:3151095/1‑148 (MQ=255)
                     tAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGa                                                                                                                         >  2:299384/1‑148 (MQ=255)
                          ctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTg                                                                                                                    <  2:263242/148‑1 (MQ=255)
                           tGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGc                                                                                                                   >  2:1722776/1‑148 (MQ=255)
                           tGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGc                                                                                                                   >  1:1993060/1‑148 (MQ=255)
                             gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCag                                                                                                                 >  1:279742/1‑148 (MQ=255)
                              aaGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCaga                                                                                                                >  2:890555/1‑148 (MQ=255)
                                gATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaag                                                                                                              >  1:2550524/1‑148 (MQ=255)
                                  tgtgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGAc                                                                                                            <  1:1642506/148‑1 (MQ=255)
                                   gtgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGAcc                                                                                                           <  2:333737/148‑1 (MQ=255)
                                      cTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAATACCGTg                                                                                                        <  2:1549807/148‑1 (MQ=255)
                                      cTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTg                                                                                                        <  1:878693/148‑1 (MQ=255)
                                       tGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGa                                                                                                       <  2:1964596/148‑1 (MQ=255)
                                        ggAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                      <  1:2374878/148‑1 (MQ=255)
                                        ggAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                      <  1:3205736/148‑1 (MQ=255)
                                        ggAAAACGTGCGTATCGAGAAGCCGAAAGGCGTGATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCGGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGTTGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                      <  1:1433185/148‑1 (MQ=255)
                                          aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACg                                                                                                    <  1:519981/148‑1 (MQ=255)
                                           aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                   <  1:3415418/148‑1 (MQ=255)
                                            aTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCt                                                                                                  >  2:2270373/1‑148 (MQ=255)
                                              cGTGCGTATCGAGAAGCCGAAAGGCTTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACGGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTc                                                                                                <  1:639425/148‑1 (MQ=255)
                                              cGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTc                                                                                                >  2:3509416/1‑148 (MQ=255)
                                                tGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCa                                                                                              >  1:659690/1‑148 (MQ=255)
                                                  cGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGATCGTGCAGAAGACCGTGAACGCTTCCAGc                                                                                            <  2:1622923/148‑1 (MQ=255)
                                                  cGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGc                                                                                            <  2:679111/148‑1 (MQ=255)
                                                        gagaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCgg                                                                                      <  1:1001338/148‑1 (MQ=255)
                                                          gaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGtt                                                                                    >  1:3466709/1‑148 (MQ=255)
                                                           aaGCCGAAAGGCGTTATCGTCCAGTACTGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                   >  1:2950136/1‑148 (MQ=255)
                                                           aaGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                   >  1:1528036/1‑148 (MQ=255)
                                                           aaGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                   >  1:1172170/1‑148 (MQ=255)
                                                           aaGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                   >  2:187496/1‑148 (MQ=255)
                                                           aaGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                   >  2:2853911/1‑148 (MQ=255)
                                                            aGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGa                                                                                  >  1:2732459/1‑148 (MQ=255)
                                                            aGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCACTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGa                                                                                  >  2:1076377/1‑148 (MQ=255)
                                                                 aaaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                             >  1:1191270/1‑148 (MQ=255)
                                                                  aaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTct                                                                            >  2:2945587/1‑148 (MQ=255)
                                                                   aGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctg                                                                           >  1:959246/1‑148 (MQ=255)
                                                                   aGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCAGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctg                                                                           >  2:93772/1‑148 (MQ=255)
                                                                    ggCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctga                                                                          <  2:3425685/148‑1 (MQ=255)
                                                                     gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                         >  1:432108/1‑148 (MQ=255)
                                                                     gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                         <  1:3076928/148‑1 (MQ=255)
                                                                      cGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaa                                                                        <  2:1067620/148‑1 (MQ=255)
                                                                       gTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaac                                                                       <  1:2453367/148‑1 (MQ=255)
                                                                        ttATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaact                                                                      <  1:285675/148‑1 (MQ=255)
                                                                         tATCGTCCTGTAGGGCGGTCAGACCCCGATGAAAGTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                     <  1:2903624/148‑1 (MQ=255)
                                                                         tATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                     <  2:309279/148‑1 (MQ=255)
                                                                           tCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaa                                                                   <  1:3160471/148‑1 (MQ=255)
                                                                            cGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaaa                                                                  >  2:1511460/1‑148 (MQ=255)
                                                                               ccAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaa                                                               >  2:2042241/1‑148 (MQ=255)
                                                                               ccAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaa                                                               >  1:1199398/1‑148 (MQ=255)
                                                                               ccAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaa                                                               >  2:2407462/1‑148 (MQ=255)
                                                                                     cggcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCg                                                         <  2:102920/148‑1 (MQ=255)
                                                                                     cggcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCg                                                         >  1:1783226/1‑148 (MQ=255)
                                                                                       gcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGaa                                                       <  2:2111057/148‑1 (MQ=255)
                                                                                              gACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCAcc                                                >  2:1833931/1‑148 (MQ=255)
                                                                                              gACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCAcc                                                >  2:1833957/1‑148 (MQ=255)
                                                                                                ccccGCTTAAAGGGGCGCGCGCGCTGGAAGCTGCGGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGt                                              <  1:2114788/148‑1 (MQ=255)
                                                                                                ccccGCTGAAACTGGCGGGCGCGCTGGACGCTGGTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGt                                              <  1:1017995/148‑1 (MQ=255)
                                                                                                     cTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCg                                         >  2:733770/1‑148 (MQ=255)
                                                                                                      tGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGc                                        <  1:299384/148‑1 (MQ=255)
                                                                                                       gAAACTGGCGCGCGCGCTGGCAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGACCGCTTCCAGCATGCGGTTGCGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGcg                                       <  1:378566/148‑2 (MQ=255)
                                                                                                       gAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCt                                       >  2:401291/1‑148 (MQ=255)
                                                                                                       gAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCt                                       >  1:2583366/1‑148 (MQ=255)
                                                                                                         aaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                     <  2:2226823/148‑1 (MQ=255)
                                                                                                              gcgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa                                <  2:2699106/148‑1 (MQ=255)
                                                                                                              gcgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa                                <  2:265671/148‑1 (MQ=255)
                                                                                                              gcgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa                                >  1:2623211/1‑148 (MQ=255)
                                                                                                               cgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAAt                               <  2:1199398/148‑1 (MQ=255)
                                                                                                               cgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAAt                               <  2:1150435/148‑1 (MQ=255)
                                                                                                               cgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAAt                               <  2:2928248/148‑1 (MQ=255)
                                                                                                               cgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAAt                               <  2:3302100/148‑1 (MQ=255)
                                                                                                                gcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATg                              <  1:3128367/148‑1 (MQ=255)
                                                                                                                 cgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATgg                             <  2:3322330/148‑1 (MQ=255)
                                                                                                                   cgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCg                           <  2:1002187/148‑1 (MQ=255)
                                                                                                                     cgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGt                         <  2:519094/148‑1 (MQ=255)
                                                                                                                       cTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTag                       <  2:1072226/148‑1 (MQ=255)
                                                                                                                          gAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga                    >  1:3120488/1‑148 (MQ=255)
                                                                                                                           aaGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa                   >  1:188564/1‑148 (MQ=255)
                                                                                                                            aGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                  <  1:3095170/148‑1 (MQ=255)
                                                                                                                            aGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                  >  2:1494693/1‑148 (MQ=255)
                                                                                                                            aGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                  >  2:2969118/1‑148 (MQ=255)
                                                                                                                              ctgctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGc                >  1:810891/1‑148 (MQ=255)
                                                                                                                               tgctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCg               <  1:1388547/148‑1 (MQ=255)
                                                                                                                                gctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGa              <  1:2367462/148‑1 (MQ=255)
                                                                                                                                 ctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGaa             <  1:1332808/148‑1 (MQ=255)
                                                                                                                                 ctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGaa             <  1:238089/148‑1 (MQ=255)
                                                                                                                                   ggCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAag           <  2:199534/148‑1 (MQ=255)
                                                                                                                                    gCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAaga          <  1:988914/148‑1 (MQ=255)
                                                                                                                                    gCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAaga          <  2:1791467/148‑1 (MQ=255)
                                                                                                                                        aCCGGTTATCGGCACCAGCCCGTATGCTATCGACCGTGAAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt      <  1:3313790/148‑1 (MQ=255)
                                                                                                                                        aCCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt      <  2:279742/148‑1 (MQ=255)
                                                                                                                                        aCCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt      >  2:2514186/1‑148 (MQ=255)
                                                                                                                                          cGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTgg    <  2:1337797/148‑1 (MQ=255)
                                                                                                                                           ggTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGc   >  2:3109262/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
ACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCT  >  CP009273/32657‑32944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑