Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,266,318 G→T 100% R307L (CGT→CTT)  yjcC → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,266,3180GT97.2% 674.8 / 9.0 217R307L (CGT→CTT) yjcCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base T (110/100);  total (112/105)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

TTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAG  >  CP009273/4266173‑4266461
                                                                                                                                                 |                                                                                                                                               
 tACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTg                                                                                                                                              >  1:331056/1‑148 (MQ=255)
 tACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTg                                                                                                                                              <  2:1782075/148‑1 (MQ=255)
   ctctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGc                                                                                                                                            <  1:1597437/148‑1 (MQ=255)
      tGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTg                                                                                                                                         >  2:2737156/1‑148 (MQ=255)
        gCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGt                                                                                                                                       <  1:2733054/148‑1 (MQ=255)
         cTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTg                                                                                                                                      <  1:3081264/148‑1 (MQ=255)
           aCGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAg                                                                                                                                    >  2:146866/1‑148 (MQ=255)
           aCGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAg                                                                                                                                    <  1:136221/148‑1 (MQ=255)
             gTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCa                                                                                                                                  >  1:2885131/1‑148 (MQ=255)
               aTTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgg                                                                                                                                >  1:2169670/1‑148 (MQ=255)
               aTTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgg                                                                                                                                >  2:562449/1‑148 (MQ=255)
                      aaaaCTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaat                                                                                                                         >  1:2192574/1‑148 (MQ=255)
                       aaaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaata                                                                                                                        <  1:194638/148‑1 (MQ=255)
                       aaaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaata                                                                                                                        <  1:3264942/148‑1 (MQ=255)
                       aaaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaata                                                                                                                        >  2:1190314/1‑148 (MQ=255)
                            aTTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGaa                                                                                                                   >  2:509460/1‑148 (MQ=255)
                             tttATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAAt                                                                                                                  <  1:3174730/148‑1 (MQ=255)
                             tttATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAAt                                                                                                                  <  1:503320/148‑1 (MQ=255)
                             tttATCTCCCAAAAGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAAt                                                                                                                  <  1:3007334/148‑1 (MQ=255)
                                aTCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCg                                                                                                               >  1:2633202/1‑148 (MQ=255)
                                     ccAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCaga                                                                                                          >  2:919320/1‑148 (MQ=255)
                                      cAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCagag                                                                                                         >  1:1959865/1‑148 (MQ=255)
                                       aaaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGt                                                                                                        >  1:2247629/1‑148 (MQ=255)
                                        aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                       >  1:2112042/1‑148 (MQ=255)
                                        aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                       >  2:3213833/1‑148 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                      <  2:1461700/148‑1 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                      <  2:2145376/148‑1 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                      <  2:2974903/148‑1 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                      >  1:1687954/1‑148 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                      <  1:769097/148‑1 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTAGAAAAACATAAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                      <  1:2294090/148‑1 (MQ=255)
                                                     aCGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAATTGTATCGGCGCTGTAGCGTTGTTACTTTGGCCTGGTGAGGAGGGGGAAATAATGAATCCGGCAGAGTTTCTTCCGCTGGCa                                                                                          >  1:2066231/1‑148 (MQ=255)
                                                         gcCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGaaa                                                                                      <  2:570922/148‑1 (MQ=255)
                                                         gcCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGaaa                                                                                      >  1:3523115/1‑148 (MQ=255)
                                                                aaaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAggg                                                                               <  2:2723955/148‑1 (MQ=255)
                                                                 aaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAgggg                                                                              >  1:923222/1‑148 (MQ=255)
                                                                  aaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGga                                                                             >  1:3118176/1‑148 (MQ=255)
                                                                  aaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGga                                                                             >  1:2505995/1‑148 (MQ=255)
                                                                   aaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgat                                                                            <  2:1716307/148‑1 (MQ=255)
                                                                   aaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgat                                                                            >  2:1818297/1‑148 (MQ=255)
                                                                    aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                           <  2:3336145/148‑1 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                          >  2:3170788/1‑148 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                          <  2:2051039/148‑1 (MQ=255)
                                                                       tCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATa                                                                        >  2:1021065/1‑148 (MQ=255)
                                                                        cAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAg                                                                       <  1:577688/148‑1 (MQ=255)
                                                                         aaCTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGa                                                                      >  2:61503/1‑148 (MQ=255)
                                                                           cTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAAc                                                                    >  2:1425555/1‑148 (MQ=255)
                                                                            tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                   <  2:403414/148‑1 (MQ=255)
                                                                               gtatttATTACCAGCTAATAATCGATTTCAAAACAGTATTTTGTATCGGCGCTGAAGCGTTTTTAGTTTGGGCTGGTGAGCAGGGGCAAATAATGAATCAGGCAGTGTTTATTCCTATTGCAGAAAAGGAGGGGATGATAGAACAGAt                                                                <  1:1046468/145‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAACCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                              <  2:2157275/148‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATat                                                              <  1:2028673/148‑2 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                              >  1:3060767/1‑148 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                              <  1:2499089/148‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                              >  1:2854472/1‑148 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                              >  1:543786/1‑148 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                              <  2:1032948/148‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATACGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                              >  2:3029917/1‑148 (MQ=255)
                                                                                  tttATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAAc                                                             >  2:852497/1‑148 (MQ=255)
                                                                                   ttattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCCGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACt                                                            >  1:613937/1‑148 (MQ=255)
                                                                                   ttattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACt                                                            >  1:959650/1‑148 (MQ=255)
                                                                                    tattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTg                                                           >  2:2416626/1‑148 (MQ=255)
                                                                                           aGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGt                                                    >  2:2389558/1‑148 (MQ=255)
                                                                                            gCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGtt                                                   >  1:2861234/1‑148 (MQ=255)
                                                                                             ccAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTa                                                  <  1:1284864/148‑1 (MQ=255)
                                                                                             ccAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTa                                                  >  2:1995331/1‑148 (MQ=255)
                                                                                             ccAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTa                                                  <  2:1882448/148‑1 (MQ=255)
                                                                                             ccAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTa                                                  <  2:1682476/148‑1 (MQ=255)
                                                                                              cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                 >  1:2204105/1‑148 (MQ=255)
                                                                                              cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                 >  1:1823591/1‑148 (MQ=255)
                                                                                               aataatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAtt                                                >  1:831173/1‑148 (MQ=255)
                                                                                                ataatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTg                                               >  1:3047589/1‑148 (MQ=255)
                                                                                                   atCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATa                                            <  2:2216679/148‑1 (MQ=255)
                                                                                                      gATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATg                                         <  2:2634346/148‑1 (MQ=255)
                                                                                                       atatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGt                                        <  2:720478/148‑1 (MQ=255)
                                                                                                        tatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTc                                       >  1:609761/1‑148 (MQ=255)
                                                                                                         atCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCt                                      <  1:2651686/148‑1 (MQ=255)
                                                                                                          tCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCtt                                     <  2:507405/148‑1 (MQ=255)
                                                                                                          tCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCtt                                     >  1:1238164/1‑148 (MQ=255)
                                                                                                           cAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTc                                    <  2:2460/148‑1 (MQ=255)
                                                                                                             aaaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcg                                  >  2:2629398/1‑148 (MQ=255)
                                                                                                             aaaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcg                                  >  2:842825/1‑148 (MQ=255)
                                                                                                              aaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                                 >  2:2032812/1‑148 (MQ=255)
                                                                                                              aaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                                 >  1:1765106/1‑148 (MQ=255)
                                                                                                              aaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                                 >  1:2815042/1‑148 (MQ=255)
                                                                                                                cAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGa                               >  1:1743220/1‑148 (MQ=255)
                                                                                                                 aGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGAt                              <  1:692003/148‑1 (MQ=255)
                                                                                                                  gAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATc                             <  2:1372495/148‑1 (MQ=255)
                                                                                                                     aaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTgg                          >  1:992633/1‑148 (MQ=255)
                                                                                                                      aaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCGGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTggg                         >  1:1510947/1‑148 (MQ=255)
                                                                                                                       aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                        >  1:2375875/1‑148 (MQ=255)
                                                                                                                       aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGAGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                        >  2:2616963/1‑148 (MQ=255)
                                                                                                                            tCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTa                   <  1:1021065/148‑1 (MQ=255)
                                                                                                                            tCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTa                   >  2:310184/1‑148 (MQ=255)
                                                                                                                               gcgcTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                >  2:2827263/1‑148 (MQ=255)
                                                                                                                                   tGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCa            <  1:1096255/148‑1 (MQ=255)
                                                                                                                                     aaGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAac          >  2:1303658/1‑148 (MQ=255)
                                                                                                                                     aaGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAac          >  2:196173/1‑148 (MQ=255)
                                                                                                                                       gCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAacac        >  1:1213357/1‑148 (MQ=255)
                                                                                                                                       gCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAacac        >  1:3071182/1‑148 (MQ=255)
                                                                                                                                             ttACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAg  <  2:1600694/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAG  >  CP009273/4266173‑4266461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑