Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,926 A→C 100% I72M (ATT→ATG)  yjcH ← DUF485 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,9260AC100.0% 820.8 / ‑6.7 204I72M (ATT→ATG) yjcHDUF485 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (113/89);  total (114/90)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

TTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTG  >  CP009273/4274781‑4275064
                                                                                                                                                 |                                                                                                                                          
ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCAt                                                                                                                                          <  1:1432577/148‑1 (MQ=255)
 tAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATc                                                                                                                                         <  1:3272427/148‑1 (MQ=255)
         aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAac                                                                                                                                 >  2:2936832/1‑148 (MQ=255)
         aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAac                                                                                                                                 >  1:3088715/1‑148 (MQ=255)
         aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAac                                                                                                                                 >  2:296431/1‑148 (MQ=255)
           aGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAacac                                                                                                                               >  2:452746/1‑148 (MQ=255)
             ggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCa                                                                                                                             <  1:2953164/148‑1 (MQ=255)
             ggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCa                                                                                                                             <  2:3496901/148‑1 (MQ=255)
                gtgtGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAtt                                                                                                                          <  2:1770406/148‑1 (MQ=255)
                 tgtgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTg                                                                                                                         >  2:422946/1‑148 (MQ=255)
                 tgtgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTg                                                                                                                         >  2:2213936/1‑148 (MQ=255)
                      cggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATa                                                                                                                    <  1:517431/148‑1 (MQ=255)
                       ggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAc                                                                                                                   <  1:2752227/148‑1 (MQ=255)
                             cgcCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCgtg                                                                                                             <  2:1535290/148‑1 (MQ=255)
                              gcCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCgtgt                                                                                                            <  2:3310928/148‑1 (MQ=255)
                                cGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGa                                                                                                          >  1:2696884/1‑148 (MQ=255)
                                  tCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCTCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACg                                                                                                        <  2:1640474/148‑1 (MQ=255)
                                  tCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACg                                                                                                        <  2:2118795/148‑1 (MQ=255)
                                   cAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGc                                                                                                       <  1:3352006/148‑1 (MQ=255)
                                    aGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCt                                                                                                      <  2:3341309/148‑1 (MQ=255)
                                        ctctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtg                                                                                                  >  2:1254555/1‑148 (MQ=255)
                                         tctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtgt                                                                                                 >  1:904071/1‑148 (MQ=255)
                                            tttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCg                                                                                              >  1:1081776/1‑148 (MQ=255)
                                            tttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCg                                                                                              >  2:295144/1‑148 (MQ=255)
                                                 tgatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTc                                                                                         >  2:145912/1‑148 (MQ=255)
                                                 tgatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTc                                                                                         <  1:1453527/148‑1 (MQ=255)
                                                 tgatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTc                                                                                         <  1:3225121/148‑1 (MQ=255)
                                                   atgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAg                                                                                       <  1:1533230/148‑1 (MQ=255)
                                                    tgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgc                                                                                      >  1:2714696/1‑148 (MQ=255)
                                                           aCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGc                                                                               >  1:2257865/1‑148 (MQ=255)
                                                             cTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                             >  2:1746225/1‑148 (MQ=255)
                                                             cTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                             >  2:3386934/1‑148 (MQ=255)
                                                              tCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                            >  2:196278/1‑148 (MQ=255)
                                                              tCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                            >  2:2500049/1‑148 (MQ=255)
                                                              tCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                            >  2:487170/1‑148 (MQ=255)
                                                               cATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccag                                                                           >  1:1337341/1‑148 (MQ=255)
                                                                   cAGGACTTCATTATTAAGACGGTCGAATTCGACGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGAGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagccag                                                                       >  2:2633475/1‑148 (MQ=255)
                                                                      gACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAACGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCg                                                                    >  2:3240636/1‑148 (MQ=255)
                                                                        cTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCggg                                                                  >  1:2920449/1‑148 (MQ=255)
                                                                         ttCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGgc                                                                 >  2:2792962/1‑148 (MQ=255)
                                                                         ttCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGgc                                                                 >  2:732617/1‑148 (MQ=255)
                                                                         ttCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGgc                                                                 >  1:2481192/1‑148 (MQ=255)
                                                                             ttattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGa                                                             >  2:2546212/1‑148 (MQ=255)
                                                                              tattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                            >  2:215925/1‑148 (MQ=255)
                                                                              tattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                            >  1:1961759/1‑148 (MQ=255)
                                                                                ttaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAgg                                                          >  2:63840/1‑148 (MQ=255)
                                                                                 taAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGc                                                         >  1:49365/1‑148 (MQ=255)
                                                                                 taAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGc                                                         >  1:1318488/1‑148 (MQ=255)
                                                                                   aGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGa                                                       <  1:1916091/148‑1 (MQ=255)
                                                                                    gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGAt                                                      <  2:378219/148‑1 (MQ=255)
                                                                                    gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGAt                                                      <  2:2882351/148‑1 (MQ=255)
                                                                                    gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGAt                                                      >  1:553151/1‑148 (MQ=255)
                                                                                     aCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATc                                                     >  1:3413512/1‑148 (MQ=255)
                                                                                        gTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATc                                                     <  1:2103553/145‑1 (MQ=255)
                                                                                         tCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                 >  1:1331379/1‑148 (MQ=255)
                                                                                            aaTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTaaaa                                              <  1:2069414/148‑1 (MQ=255)
                                                                                             aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                             >  2:3347404/1‑148 (MQ=255)
                                                                                             aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                             >  2:2010471/1‑148 (MQ=255)
                                                                                              ttCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATa                                            >  2:2396147/1‑148 (MQ=255)
                                                                                              ttCGCCGTTC‑CACGCAAGATGTAGATACCGGTGAGAACAAAGTAGAACACAATCACACCAACACCAATTTGAAAACCTCGTGTGACGCAGGTGTTCGGAATCACCGGCGAGCCCAGCCAGCAGGGCGCGAAGGCGATCAGTAAAATaa                                           >  2:2096394/1‑148 (MQ=255)
                                                                                               tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATaa                                           >  2:516918/1‑148 (MQ=255)
                                                                                               tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATaa                                           <  1:652701/148‑1 (MQ=255)
                                                                                                cGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATaaa                                          <  1:2308074/148‑1 (MQ=255)
                                                                                                 gCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAg                                         <  1:437839/148‑1 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                        <  1:904889/148‑1 (MQ=255)
                                                                                                     ttCGCCCGCCAGATGTAGATACCGGTGAGAACAACTGAGATCACCATCACTCCAACCCCATTTTGAATACCGCGTGTGACGCTTGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAATGCGATCAGTTAAATAAAGCCGa                                     <  2:1996135/148‑1 (MQ=255)
                                                                                                         cccgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGAtata                                 <  1:1105697/148‑1 (MQ=255)
                                                                                                          ccgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCGGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaa                                <  1:2342783/148‑1 (MQ=255)
                                                                                                           cgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaaa                               <  1:296431/148‑1 (MQ=255)
                                                                                                           cgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaaa                               <  1:215925/148‑1 (MQ=255)
                                                                                                           cgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaaa                               <  1:3418383/148‑1 (MQ=255)
                                                                                                             ccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACt                             >  1:803061/1‑148 (MQ=255)
                                                                                                              cAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTg                            <  1:2308380/148‑1 (MQ=255)
                                                                                                              cAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTg                            <  1:3263322/148‑1 (MQ=255)
                                                                                                                   gTAGATACCGGTGAGAGCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGc                       >  1:383635/1‑148 (MQ=255)
                                                                                                                    tAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCa                      <  1:487170/148‑1 (MQ=255)
                                                                                                                      gATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCata                    >  2:3432244/1‑148 (MQ=255)
                                                                                                                      gATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCata                    >  2:2193760/1‑148 (MQ=255)
                                                                                                                       aTACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCCGCataa                   >  2:3296119/1‑148 (MQ=255)
                                                                                                                        tACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCCATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataat                  <  1:930688/148‑1 (MQ=255)
                                                                                                                        tACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataat                  >  2:1195961/1‑148 (MQ=255)
                                                                                                                        gaCCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataat                  <  1:962277/147‑1 (MQ=255)
                                                                                                                          ccGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCAtaataa                <  1:290128/148‑1 (MQ=255)
                                                                                                                              tGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGa            <  2:1938720/148‑1 (MQ=255)
                                                                                                                              tGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGa            >  2:1531618/1‑148 (MQ=255)
                                                                                                                              tGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGa            <  2:2539105/148‑1 (MQ=255)
                                                                                                                              tGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGa            >  2:1531631/1‑148 (MQ=255)
                                                                                                                               gagaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGAc           >  2:3108162/1‑148 (MQ=255)
                                                                                                                                agaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACa          >  1:2046536/1‑148 (MQ=255)
                                                                                                                                agaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACa          <  1:3169900/148‑1 (MQ=255)
                                                                                                                                 gaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAg         >  1:1698766/1‑148 (MQ=255)
                                                                                                                                  aaCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAgg        >  1:472157/1‑148 (MQ=255)
                                                                                                                                    cAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAt      >  2:3044028/1‑148 (MQ=255)
                                                                                                                                    cAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAt      >  1:2114873/1‑148 (MQ=255)
                                                                                                                                    cAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAt      >  1:103390/1‑148 (MQ=255)
                                                                                                                                       aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt   >  2:2979714/1‑148 (MQ=255)
                                                                                                                                       aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt   >  2:2280891/1‑148 (MQ=255)
                                                                                                                                       aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt   >  1:2580332/1‑148 (MQ=255)
                                                                                                                                       aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt   >  2:492828/1‑148 (MQ=255)
                                                                                                                                        ggAGATCACCAGCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggtg  <  2:702945/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
TTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTG  >  CP009273/4274781‑4275064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑