Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,288,086 G→T 100% F366L (TTC→TTA)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,288,0860GT96.5% 518.0 / 9.5 170F366L (TTC→TTA) fdhFformate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (75/89);  total (78/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 170 total aligned reads displayed.

CTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGG  >  CP009273/4287941‑4288228
                                                                                                                                                 |                                                                                                                                              
cTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTaa                                                                                                                                              >  2:1750099/1‑148 (MQ=255)
    cGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtc                                                                                                                                          <  1:2250308/148‑1 (MQ=255)
    cGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtc                                                                                                                                          <  1:1341909/148‑1 (MQ=255)
    cGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtc                                                                                                                                          <  1:1342054/148‑1 (MQ=255)
     gACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtct                                                                                                                                         >  1:3375422/1‑148 (MQ=255)
          cTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCgg                                                                                                                                    >  2:2696595/1‑148 (MQ=255)
          cTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCgg                                                                                                                                    <  1:1520505/148‑1 (MQ=255)
          cTCCGCGTAAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCgg                                                                                                                                    <  1:1109759/148‑1 (MQ=255)
             cgcgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTa                                                                                                                                 >  1:2480067/1‑148 (MQ=255)
               cgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGc                                                                                                                               >  2:2533085/1‑148 (MQ=255)
               cgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGc                                                                                                                               <  1:2118557/148‑1 (MQ=255)
               cgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGc                                                                                                                               <  1:2118545/148‑1 (MQ=255)
                 tCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCg                                                                                                                             >  2:1825615/1‑148 (MQ=255)
                 tCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCg                                                                                                                             >  2:280785/1‑148 (MQ=255)
                     tttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATc                                                                                                                         >  2:2515516/1‑148 (MQ=255)
                     tttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATc                                                                                                                         >  1:3096279/1‑148 (MQ=255)
                     tttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGACATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATc                                                                                                                         <  1:1355280/148‑1 (MQ=255)
                      ttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCt                                                                                                                        <  2:449596/148‑1 (MQ=255)
                      ttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCt                                                                                                                        <  1:694704/148‑1 (MQ=255)
                        gTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCAGGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCttt                                                                                                                      <  2:317269/148‑1 (MQ=255)
                         tAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTc                                                                                                                     <  2:2798476/148‑1 (MQ=255)
                                tCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                              >  2:2696219/1‑148 (MQ=255)
                                tCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                              >  1:2352920/1‑148 (MQ=255)
                                tCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                              <  1:2562145/148‑1 (MQ=255)
                                 cTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTg                                                                                                             >  2:379673/1‑148 (MQ=255)
                                 cTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTg                                                                                                             >  2:2171551/1‑148 (MQ=255)
                                 cTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTg                                                                                                             >  2:2171632/1‑148 (MQ=255)
                                   tCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGt                                                                                                           <  1:2227339/148‑1 (MQ=255)
                                      cccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                        <  1:837074/148‑1 (MQ=255)
                                      cccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                        <  1:1288904/148‑1 (MQ=255)
                                      cccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                        >  1:2175628/1‑148 (MQ=255)
                                      cccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGAGAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                        <  1:1681620/148‑1 (MQ=255)
                                       ccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGAGAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATcc                                                                                                       <  2:2320542/148‑1 (MQ=255)
                                         aTAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCg                                                                                                     >  2:2879895/1‑148 (MQ=255)
                                         aTAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCg                                                                                                     >  2:1922064/1‑148 (MQ=255)
                                            aTGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGAt                                                                                                  <  1:3228103/148‑1 (MQ=255)
                                             tGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATa                                                                                                 <  2:1598216/148‑1 (MQ=255)
                                               tACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGCTAGCCGGATCTTTCACGTACTGGTATCCCGGATACg                                                                                               <  1:702464/148‑1 (MQ=255)
                                                 cgcgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTa                                                                                             <  2:2214142/148‑1 (MQ=255)
                                                  gcgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTAt                                                                                            <  2:455069/148‑1 (MQ=255)
                                                   cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                           >  2:1166979/1‑148 (MQ=255)
                                                   cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                           >  1:2447220/1‑148 (MQ=255)
                                                     gcacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCg                                                                                         >  1:2675011/1‑148 (MQ=255)
                                                     gcacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCGTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCg                                                                                         >  2:2361682/1‑148 (MQ=255)
                                                      cacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCgg                                                                                        >  1:2571636/1‑148 (MQ=255)
                                                       acgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGc                                                                                       <  2:1471835/148‑1 (MQ=255)
                                                             ttCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgcg                                                                                 >  2:3406677/1‑148 (MQ=255)
                                                                   aTGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCata                                                                           <  1:3274987/148‑1 (MQ=255)
                                                                    tgcgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCatat                                                                          <  1:122759/148‑1 (MQ=255)
                                                                    tgcgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCatat                                                                          >  1:487716/1‑148 (MQ=255)
                                                                     gcgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTNGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATc                                                                         >  1:1806274/1‑148 (MQ=255)
                                                                      cgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCg                                                                        <  1:904940/148‑1 (MQ=255)
                                                                       gctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGc                                                                       <  2:3419461/148‑1 (MQ=255)
                                                                       gctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGc                                                                       <  1:2370923/148‑1 (MQ=255)
                                                                          gcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAgg                                                                    <  1:1503445/148‑1 (MQ=255)
                                                                          gcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAgg                                                                    <  1:629795/148‑1 (MQ=255)
                                                                            gcgGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCACAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATAACGCAGGCa                                                                  <  1:1624358/148‑1 (MQ=255)
                                                                                tGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCt                                                              >  2:2563687/1‑148 (MQ=255)
                                                                                  cGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGa                                                            <  1:1642061/148‑1 (MQ=255)
                                                                                  cGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGa                                                            <  1:1191033/148‑1 (MQ=255)
                                                                                  cGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGa                                                            >  2:1166074/1‑148 (MQ=255)
                                                                                   ggCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGaa                                                           <  2:3314198/148‑1 (MQ=255)
                                                                                     cAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACg                                                         <  2:2520325/148‑1 (MQ=255)
                                                                                     cAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACg                                                         >  2:1937133/1‑148 (MQ=255)
                                                                                     cAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACg                                                         >  1:2531248/1‑148 (MQ=255)
                                                                                         tCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttgt                                                     <  2:166736/148‑1 (MQ=255)
                                                                                          cGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttgtt                                                    >  2:1312235/1‑148 (MQ=255)
                                                                                           gCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTc                                                   >  2:2015685/1‑148 (MQ=255)
                                                                                              gATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGa                                                >  2:2854641/1‑148 (MQ=255)
                                                                                               aTGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGAc                                               >  1:2708922/1‑148 (MQ=255)
                                                                                               aTGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGGTCTGAc                                               >  2:2363522/1‑148 (MQ=255)
                                                                                                 gCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCa                                             <  1:1993648/148‑1 (MQ=255)
                                                                                                 gCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGTACGTTGTTCTGACCa                                             <  2:2497607/148‑1 (MQ=255)
                                                                                                     tAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGaa                                         >  2:2637349/1‑148 (MQ=255)
                                                                                                       gCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAcc                                       >  1:3124114/1‑148 (MQ=255)
                                                                                                             aTGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTa                                 <  2:1382777/148‑1 (MQ=255)
                                                                                                                     gCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGc                         >  1:300393/1‑148 (MQ=255)
                                                                                                                     gCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGc                         >  1:3495076/1‑148 (MQ=255)
                                                                                                                       aGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACGGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCAt                       <  2:847266/148‑1 (MQ=255)
                                                                                                                            ttCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGTCAGCGCGCACATATCGCAGGCACCATGAACGTTGTACTGACCACGAACCGCTTTAACAACCGCATGCgtc                  >  2:2077381/1‑146 (MQ=255)
                                                                                                                            ttCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGc                  >  2:1090024/1‑148 (MQ=255)
                                                                                                                             tCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCt                 <  2:1940251/148‑1 (MQ=255)
                                                                                                                               cACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTa               <  2:2571592/148‑1 (MQ=255)
                                                                                                                               cACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTa               <  2:2571636/148‑1 (MQ=255)
                                                                                                                               cACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTa               >  2:1371629/1‑148 (MQ=255)
                                                                                                                                aCGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAc              >  1:1142326/1‑148 (MQ=255)
                                                                                                                                  gCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCg            <  1:855538/148‑1 (MQ=255)
                                                                                                                                     ccAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAgg         <  2:192528/148‑1 (MQ=255)
                                                                                                                                     ccAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAgg         >  1:3333230/1‑148 (MQ=255)
                                                                                                                                     ccAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAgg         <  2:1994300/148‑1 (MQ=255)
                                                                                                                                      cAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGt        <  2:3375422/148‑1 (MQ=255)
                                                                                                                                      cAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGt        <  1:2581016/148‑1 (MQ=255)
                                                                                                                                        ggCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTa      <  2:1826593/148‑1 (MQ=255)
                                                                                                                                        ggCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGtga      >  2:490993/1‑146 (MQ=255)
                                                                                                                                        ggCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTa      >  2:1558193/1‑148 (MQ=255)
                                                                                                                                         gCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAc     <  1:379673/148‑1 (MQ=255)
                                                                                                                                         gCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAc     >  1:2626646/1‑148 (MQ=255)
                                                                                                                                         gCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAc     <  1:2171551/148‑1 (MQ=255)
                                                                                                                                          cTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATCTCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAcc    <  2:3502230/148‑1 (MQ=255)
                                                                                                                                          cTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAcc    >  1:1891297/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGG  >  CP009273/4287941‑4288228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑