Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,290,080 G→T 100% I256I (ATC→ATA)  mdtP ← outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,290,0800GT100.0% 593.1 / ‑6.7 182I256I (ATC→ATA) mdtPouter membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (76/105);  total (76/106)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

GCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGT  >  CP009273/4289935‑4290224
                                                                                                                                                 |                                                                                                                                                
gCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAt                                                                                                                                                <  1:3259848/148‑1 (MQ=255)
 cAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAtt                                                                                                                                               >  2:1994036/1‑148 (MQ=255)
 cAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAtt                                                                                                                                               <  1:3320402/148‑1 (MQ=255)
    aTCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGc                                                                                                                                            >  2:2129755/1‑148 (MQ=255)
    aTCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGc                                                                                                                                            >  2:1385526/1‑148 (MQ=255)
     tCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGcc                                                                                                                                           >  1:2843971/1‑148 (MQ=255)
      ccGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGccc                                                                                                                                          <  1:2030295/148‑1 (MQ=255)
        gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCtt                                                                                                                                        <  1:2652008/148‑1 (MQ=255)
        gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCtt                                                                                                                                        <  1:2078818/148‑1 (MQ=255)
        gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCtt                                                                                                                                        <  1:2078807/148‑1 (MQ=255)
         ggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCttt                                                                                                                                       >  2:634803/1‑148 (MQ=255)
         ggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCttt                                                                                                                                       >  2:2735790/1‑148 (MQ=255)
         ggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCttt                                                                                                                                       >  1:912759/1‑148 (MQ=255)
          gCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                      >  2:338191/1‑148 (MQ=255)
            gTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGAc                                                                                                                                    <  2:971955/148‑1 (MQ=255)
               tGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGc                                                                                                                                 <  1:3470105/148‑1 (MQ=255)
               tGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGc                                                                                                                                 <  1:470091/148‑1 (MQ=255)
                    aGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaacaa                                                                                                                            <  2:1512415/148‑1 (MQ=255)
                         cTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGt                                                                                                                       <  1:693855/148‑1 (MQ=255)
                         cTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGt                                                                                                                       <  1:1098054/148‑1 (MQ=255)
                         cTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGt                                                                                                                       <  1:1724586/148‑1 (MQ=255)
                          tCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCATTGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGtt                                                                                                                      <  1:2254578/148‑1 (MQ=255)
                           cATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGttt                                                                                                                     <  2:1425637/148‑1 (MQ=255)
                             tAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTAt                                                                                                                   <  2:115829/148‑1 (MQ=255)
                             tAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTAt                                                                                                                   <  2:3528074/148‑1 (MQ=255)
                              aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATc                                                                                                                  >  2:2261730/1‑148 (MQ=255)
                               agagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                                                 >  2:2023547/1‑148 (MQ=255)
                                gagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                                                >  1:421895/1‑148 (MQ=255)
                                gagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                                                >  1:2907987/1‑148 (MQ=255)
                                 agagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                                               <  1:1839207/148‑1 (MQ=255)
                                     tgtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGcc                                                                                                           >  1:2907095/1‑148 (MQ=255)
                                          ccGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAAt                                                                                                      <  2:1044914/148‑1 (MQ=255)
                                            ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCt                                                                                                    >  2:3014019/1‑148 (MQ=255)
                                              aaTGTCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGa                                                                                                        <  1:2311375/142‑1 (MQ=255)
                                              aaTGTCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGa                                                                                                        >  2:2311375/1‑142 (MQ=255)
                                               aTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgtg                                                                                                 <  1:1940194/148‑1 (MQ=255)
                                                tGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGc                                                                                                >  1:359640/1‑148 (MQ=255)
                                                 gCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGcc                                                                                               <  2:3225699/148‑1 (MQ=255)
                                                 gCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGcc                                                                                               <  2:1161312/148‑1 (MQ=255)
                                                         ggACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAGTCTGTGCCCGCGCGcc                                                                                       >  2:665125/1‑148 (MQ=255)
                                                             ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTgg                                                                                   >  2:2858891/1‑148 (MQ=255)
                                                               gCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCCATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaa                                                                                 <  2:3159202/148‑1 (MQ=255)
                                                               gCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaa                                                                                 <  1:2299699/148‑1 (MQ=255)
                                                               gCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaa                                                                                 <  2:3158639/148‑1 (MQ=255)
                                                                 ggTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAg                                                                               <  1:1480403/148‑1 (MQ=255)
                                                                  gTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAgg                                                                              <  1:928249/148‑1 (MQ=255)
                                                                     aTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCAc                                                                           <  1:278855/148‑1 (MQ=255)
                                                                      tGCCACCGGTCTGATCTCCGTCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACt                                                                          <  2:654557/148‑1 (MQ=255)
                                                                           ccGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcgc                                                                     <  2:2520955/148‑1 (MQ=255)
                                                                             ggTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCtt                                                                   >  2:3511096/1‑148 (MQ=255)
                                                                             ggTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCtt                                                                   >  2:394299/1‑148 (MQ=255)
                                                                              gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTc                                                                  >  1:2929702/1‑148 (MQ=255)
                                                                              gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTc                                                                  <  2:1175399/148‑1 (MQ=255)
                                                                                cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                                                >  1:407236/1‑148 (MQ=255)
                                                                                  gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGa                                                              >  1:100762/1‑148 (MQ=255)
                                                                                   aTCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAc                                                             >  2:235475/1‑148 (MQ=255)
                                                                                    tctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAcc                                                            <  1:2043057/148‑1 (MQ=255)
                                                                                    tctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAcc                                                            >  1:2141849/1‑148 (MQ=255)
                                                                                     ctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCg                                                           <  1:2468915/148‑1 (MQ=255)
                                                                                     ctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCg                                                           >  2:2860985/1‑148 (MQ=255)
                                                                                       ccGGCATATCGCTGGCTCCCGCGCCAATCACTGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTg                                                         <  2:2540387/148‑1 (MQ=255)
                                                                                          gCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcgc                                                      <  1:1689645/148‑1 (MQ=255)
                                                                                           cATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGcc                                                     >  2:1602464/1‑148 (MQ=255)
                                                                                           cATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGcc                                                     >  1:461929/1‑148 (MQ=255)
                                                                                             tatCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCAc                                                   >  1:2274246/1‑148 (MQ=255)
                                                                                                cGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACttt                                                <  2:2391757/148‑1 (MQ=255)
                                                                                                cGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACttt                                                <  2:2709843/148‑1 (MQ=255)
                                                                                                 gCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTa                                               <  2:2843971/148‑1 (MQ=255)
                                                                                                  cTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTAc                                              >  1:1204039/1‑148 (MQ=255)
                                                                                                   tGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTAct                                             <  2:359640/148‑1 (MQ=255)
                                                                                                    ggCTCCCGCGCCAATCACTGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActc                                            <  1:2469614/148‑1 (MQ=255)
                                                                                                    ggCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActc                                            >  2:1552304/1‑148 (MQ=255)
                                                                                                     gCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActct                                           >  1:1857573/1‑148 (MQ=255)
                                                                                                      cTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTg                                          <  2:2026375/148‑1 (MQ=255)
                                                                                                       tCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTgg                                         <  2:2907987/148‑1 (MQ=255)
                                                                                                       tCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTgg                                         >  1:1290551/1‑148 (MQ=255)
                                                                                                            cgcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgcgc                                    >  2:1853549/1‑148 (MQ=255)
                                                                                                             gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCt                                   <  1:3462386/148‑1 (MQ=255)
                                                                                                             gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCt                                   <  1:2735790/148‑1 (MQ=255)
                                                                                                             gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCt                                   <  2:869440/148‑1 (MQ=255)
                                                                                                             gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCt                                   <  2:296437/148‑1 (MQ=255)
                                                                                                               caatcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCttt                                 >  2:766927/1‑148 (MQ=255)
                                                                                                               caatcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCttt                                 <  1:236950/148‑1 (MQ=255)
                                                                                                                      tGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcgcg                          <  2:433331/148‑1 (MQ=255)
                                                                                                                        cACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTa                        <  1:1421567/148‑1 (MQ=255)
                                                                                                                         aCGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaa                       <  1:1601132/148‑1 (MQ=255)
                                                                                                                            cAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatca                    <  2:509890/148‑1 (MQ=255)
                                                                                                                              gagaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaat                  >  2:1709517/1‑148 (MQ=255)
                                                                                                                                gaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAatcaatca                <  1:2262642/148‑1 (MQ=255)
                                                                                                                                gaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAatcaatca                <  1:497192/148‑1 (MQ=255)
                                                                                                                                 aTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATcac               <  1:1614614/148‑1 (MQ=255)
                                                                                                                                 aTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATcac               <  2:3464600/148‑1 (MQ=255)
                                                                                                                                   tctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACAt             >  2:1325049/1‑148 (MQ=255)
                                                                                                                                   tctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACAt             <  1:394299/148‑1 (MQ=255)
                                                                                                                                     tcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCg           <  1:2858895/148‑1 (MQ=255)
                                                                                                                                     tcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCg           <  1:1552304/148‑1 (MQ=255)
                                                                                                                                      cgcgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGt          >  1:221052/1‑148 (MQ=255)
                                                                                                                                      cgcgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGt          >  1:642280/1‑148 (MQ=255)
                                                                                                                                           ttCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGtt     >  2:1663108/1‑148 (MQ=255)
                                                                                                                                              ggTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGt  <  2:1660505/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGT  >  CP009273/4289935‑4290224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑