Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,297,846 C→A 100% M150I (ATG→ATT)  alsE ← allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,8460CA98.8% 805.8 / 0.3 248M150I (ATG→ATT) alsEallulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (113/132);  total (113/135)
Fisher's exact test for biased strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

GGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGG  >  CP009273/4297701‑4297990
                                                                                                                                                 |                                                                                                                                                
 gCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAtt                                                                                                                                               >  1:2251805/1‑148 (MQ=255)
 gCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAtt                                                                                                                                               >  1:2702450/1‑148 (MQ=255)
  ccGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAttt                                                                                                                                              <  1:834231/148‑1 (MQ=255)
   cGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTc                                                                                                                                             <  1:13687/148‑1 (MQ=255)
    ggAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCa                                                                                                                                            <  2:3190358/148‑1 (MQ=255)
      aaGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAgg                                                                                                                                          <  1:2508028/148‑1 (MQ=255)
      aaGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAgg                                                                                                                                          >  2:1164098/1‑148 (MQ=255)
           ccGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGa                                                                                                                                     <  2:2548629/148‑1 (MQ=255)
           ccGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGa                                                                                                                                     <  2:1625474/148‑1 (MQ=255)
            cgacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGaa                                                                                                                                    <  1:576963/148‑1 (MQ=255)
             gacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAAc                                                                                                                                   >  2:661868/1‑148 (MQ=255)
                gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGt                                                                                                                                <  1:1666656/148‑1 (MQ=255)
                  tAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTg                                                                                                                              >  2:3520365/1‑148 (MQ=255)
                   aaaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGt                                                                                                                             <  2:1026934/148‑1 (MQ=255)
                          tCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCaa                                                                                                                      >  1:517345/1‑148 (MQ=255)
                           ccGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCaaa                                                                                                                     >  2:420370/1‑148 (MQ=255)
                             gCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGc                                                                                                                   <  2:1311625/148‑1 (MQ=255)
                              ccccTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGcc                                                                                                                  <  1:3094136/148‑1 (MQ=255)
                               cccTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCg                                                                                                                 <  1:225288/148‑1 (MQ=255)
                                 cTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCggg                                                                                                               >  1:896663/1‑148 (MQ=255)
                                  tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGa                                                                                                              >  2:3000181/1‑148 (MQ=255)
                                  tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCGTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGa                                                                                                              <  2:1911825/148‑1 (MQ=255)
                                   gccgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGAt                                                                                                             <  2:1575793/148‑1 (MQ=255)
                                     cgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCg                                                                                                           <  2:1942266/148‑1 (MQ=255)
                                      gccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGa                                                                                                          <  1:186838/148‑1 (MQ=255)
                                       ccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGAc                                                                                                         >  2:763746/1‑148 (MQ=255)
                                        cATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGCTCGACa                                                                                                        >  2:2174775/1‑148 (MQ=255)
                                        cATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACa                                                                                                        >  2:375992/1‑148 (MQ=255)
                                         aTCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAg                                                                                                       >  1:19358/1‑148 (MQ=255)
                                          tCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGt                                                                                                      >  1:875364/1‑148 (MQ=255)
                                          tCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGt                                                                                                      >  2:3149353/1‑148 (MQ=255)
                                          tCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGATTGTCCGGCAAAGCCGGGATCGACAGt                                                                                                      >  1:1885037/1‑148 (MQ=255)
                                           cAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                     <  2:1783831/148‑1 (MQ=255)
                                           cAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                     <  2:2939895/148‑1 (MQ=255)
                                           cAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                     >  1:505827/1‑148 (MQ=255)
                                            aGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCa                                                                                                    <  2:612268/148‑1 (MQ=255)
                                             gTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCAt                                                                                                   >  1:243490/1‑148 (MQ=255)
                                             gTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCAt                                                                                                   >  1:669438/1‑148 (MQ=255)
                                              tttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATg                                                                                                  <  1:3409131/148‑1 (MQ=255)
                                              tttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATg                                                                                                  <  2:3028152/148‑1 (MQ=255)
                                               ttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGa                                                                                                 >  2:2042616/1‑148 (MQ=255)
                                                  tCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCg                                                                                              >  2:549248/1‑148 (MQ=255)
                                                    gTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTa                                                                                            <  2:2111362/148‑1 (MQ=255)
                                                       aGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtt                                                                                         <  1:1628186/148‑1 (MQ=255)
                                                         ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtttt                                                                                       <  1:3405629/148‑1 (MQ=255)
                                                         ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtttt                                                                                       >  2:569196/1‑148 (MQ=255)
                                                                gTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGcc                                                                                >  2:1688352/1‑148 (MQ=255)
                                                                  tGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCtt                                                                              <  1:1171937/148‑1 (MQ=255)
                                                                  tGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCtt                                                                              <  1:235372/148‑1 (MQ=255)
                                                                      ggAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATgg                                                                          <  2:3127354/148‑1 (MQ=255)
                                                                      ggAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATgg                                                                          <  2:3404179/148‑1 (MQ=255)
                                                                      ggAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATgg                                                                          <  2:3404247/148‑1 (MQ=255)
                                                                        aaCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGat                                                                        <  1:324176/148‑1 (MQ=255)
                                                                        aaCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGat                                                                        <  2:492250/148‑1 (MQ=255)
                                                                        aaCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGat                                                                        <  1:29371/148‑1 (MQ=255)
                                                                          ccGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGatat                                                                      <  1:2116836/148‑1 (MQ=255)
                                                                               cACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAt                                                                 >  2:1868714/1‑148 (MQ=255)
                                                                                  cTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTc                                                              >  1:2552765/1‑148 (MQ=255)
                                                                                   tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCa                                                             <  1:172789/148‑1 (MQ=255)
                                                                                   tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCa                                                             >  2:2391940/1‑148 (MQ=255)
                                                                                    cAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCAt                                                            >  1:380970/1‑148 (MQ=255)
                                                                                    cAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCAt                                                            >  1:2957804/1‑148 (MQ=255)
                                                                                      aTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATgg                                                          >  2:1144178/1‑148 (MQ=255)
                                                                                       tttCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                         <  2:3045117/148‑1 (MQ=255)
                                                                                       tttCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                         >  2:417889/1‑148 (MQ=255)
                                                                                        ttCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGcc                                                        <  1:3337879/148‑1 (MQ=255)
                                                                                        ttCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGcc                                                        >  1:3434635/1‑148 (MQ=255)
                                                                                            tACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCaa                                                    >  1:1975380/1‑148 (MQ=255)
                                                                                             aCTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAAc                                                   <  1:2874203/148‑1 (MQ=255)
                                                                                               tCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTg                                                 >  2:2181745/1‑148 (MQ=255)
                                                                                                  aGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCg                                              <  2:3502458/148‑1 (MQ=255)
                                                                                                     ccTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGtct                                           <  1:2636732/148‑1 (MQ=255)
                                                                                                      cTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGtctc                                          >  2:2354741/1‑148 (MQ=255)
                                                                                                        tctcGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCg                                        <  1:3502080/148‑1 (MQ=255)
                                                                                                         ctcGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCgg                                       >  1:1054697/1‑148 (MQ=255)
                                                                                                             ttCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTa                                   <  2:498038/148‑1 (MQ=255)
                                                                                                                 cGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGa                               <  2:2898558/148‑1 (MQ=255)
                                                                                                                   ccATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAGTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATc                             >  2:2687516/1‑148 (MQ=255)
                                                                                                                      tGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGc                          <  1:1688471/148‑1 (MQ=255)
                                                                                                                       gCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcc                         >  1:1079258/1‑148 (MQ=255)
                                                                                                                          ttCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                      <  1:350618/148‑1 (MQ=255)
                                                                                                                          ttCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                      <  1:549248/148‑1 (MQ=255)
                                                                                                                          ttCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                      <  1:353854/148‑1 (MQ=255)
                                                                                                                            cAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACt                    <  2:3460939/148‑1 (MQ=255)
                                                                                                                             aGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGTCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACtt                   <  1:3111186/148‑1 (MQ=255)
                                                                                                                               ttCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtc                 >  2:317985/1‑148 (MQ=255)
                                                                                                                               ttCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtc                 >  2:1737495/1‑148 (MQ=255)
                                                                                                                                 cGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcat               >  1:1776468/1‑148 (MQ=255)
                                                                                                                                   gCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgt             <  1:3421034/148‑1 (MQ=255)
                                                                                                                                    cAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtc            >  1:722403/1‑148 (MQ=255)
                                                                                                                                     aaGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtca           <  1:1295544/148‑1 (MQ=255)
                                                                                                                                     aaGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtca           <  1:990597/148‑1 (MQ=255)
                                                                                                                                       gTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcatg         >  1:2339281/1‑148 (MQ=255)
                                                                                                                                       gTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcatg         <  1:2391940/148‑1 (MQ=255)
                                                                                                                                        tttATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcatgt        >  1:2020304/1‑147 (MQ=255)
                                                                                                                                        tttATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGa        <  2:517270/148‑1 (MQ=255)
                                                                                                                                        tttATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGa        <  2:517397/148‑1 (MQ=255)
                                                                                                                                        tttATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGa        >  2:1451808/1‑148 (MQ=255)
                                                                                                                                              cAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcgg  <  2:942961/148‑1 (MQ=255)
                                                                                                                                              cAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcgg  <  1:1077021/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGG  >  CP009273/4297701‑4297990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑