Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 344,800 G→T 100% E136D (GAG→GAT)  prpB → 2‑methylisocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273344,8000GT100.0% 523.6 / NA 163E136D (GAG→GAT) prpB2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (79/84);  total (79/84)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATG  >  CP009273/344655‑344944
                                                                                                                                                 |                                                                                                                                                
tCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGatat                                                                                                                                                >  1:1898552/1‑148 (MQ=255)
 cGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtg                                                                                                                                               >  1:3260461/1‑148 (MQ=255)
 cGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtg                                                                                                                                               >  2:3060313/1‑148 (MQ=255)
   gTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggt                                                                                                                                             <  2:1706251/148‑1 (MQ=255)
   gTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggt                                                                                                                                             >  1:2927731/1‑148 (MQ=255)
       tGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggat                                                                                                                                         >  1:1038775/1‑148 (MQ=255)
       tGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggat                                                                                                                                         <  1:2125500/148‑1 (MQ=255)
          ttcttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcgg                                                                                                                                      <  1:2340659/148‑1 (MQ=255)
          ttcttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcgg                                                                                                                                      <  1:2278275/148‑1 (MQ=255)
             ttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcggatc                                                                                                                                   >  2:507974/1‑148 (MQ=255)
             ttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcggatc                                                                                                                                   >  2:837638/1‑148 (MQ=255)
              tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATcc                                                                                                                                  <  2:2862910/148‑1 (MQ=255)
              tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATcc                                                                                                                                  <  2:2862677/148‑1 (MQ=255)
                 gCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCcgcg                                                                                                                               >  1:1232448/1‑148 (MQ=255)
                  ccTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCcgcgc                                                                                                                              >  1:1500336/1‑148 (MQ=255)
                  ccTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCcgcgc                                                                                                                              >  1:1698658/1‑148 (MQ=255)
                  ccTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCcgcgc                                                                                                                              >  2:1186122/1‑148 (MQ=255)
                   cTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCcgcgcg                                                                                                                             <  1:2083423/148‑1 (MQ=255)
                    tttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCgcgg                                                                                                                            >  1:3535916/1‑148 (MQ=255)
                        aCGTGGCGCGCCCCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGt                                                                                                                        <  1:2568839/148‑1 (MQ=255)
                        aCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGt                                                                                                                        <  1:325809/148‑1 (MQ=255)
                        aCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGt                                                                                                                        <  1:365396/148‑1 (MQ=255)
                        aCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGt                                                                                                                        <  1:1314101/148‑1 (MQ=255)
                          gTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTgg                                                                                                                      <  2:3410564/148‑1 (MQ=255)
                           tGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGa                                                                                                                     <  1:2870948/148‑1 (MQ=255)
                             gcgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATg                                                                                                                   <  1:713230/148‑1 (MQ=255)
                                      gTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCg                                                                                                          <  2:3018720/148‑1 (MQ=255)
                                      gTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCg                                                                                                          >  2:179790/1‑148 (MQ=255)
                                      gTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCg                                                                                                          >  2:1980228/1‑148 (MQ=255)
                                      gTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCg                                                                                                          <  2:2739376/148‑1 (MQ=255)
                                       tGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGa                                                                                                         <  1:2000278/148‑1 (MQ=255)
                                        gAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGAt                                                                                                        <  1:3071845/148‑1 (MQ=255)
                                         aaaTCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATc                                                                                                       <  1:251024/148‑1 (MQ=255)
                                           aTCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCt                                                                                                     <  1:78874/148‑1 (MQ=255)
                                            tCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTg                                                                                                    <  1:1864116/148‑1 (MQ=255)
                                             cGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGa                                                                                                   <  2:1450437/148‑1 (MQ=255)
                                             cGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGa                                                                                                   <  2:1339848/148‑1 (MQ=255)
                                              gatgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAt                                                                                                  <  2:683739/148‑1 (MQ=255)
                                               atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                                                                                                 <  2:2584624/148‑1 (MQ=255)
                                               atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                                                                                                 <  2:2989041/148‑1 (MQ=255)
                                                        gCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCa                                                                                        >  2:3243712/1‑148 (MQ=255)
                                                        gCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCa                                                                                        <  1:678179/148‑1 (MQ=255)
                                                         ccGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCAt                                                                                       <  2:1973492/148‑1 (MQ=255)
                                                         ccGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCAt                                                                                       >  1:413444/1‑148 (MQ=255)
                                                           ggTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATgg                                                                                     <  1:1719216/148‑1 (MQ=255)
                                                                  cAGGATTGCATATTGAAGATCAGGTTGGTGCGCAACGCTGCGGTCATCGTCCGAATCAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAc                                                                              <  2:2824413/148‑1 (MQ=255)
                                                                     gATTGCATATTGAAGCTCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGa                                                                           <  1:2119473/148‑1 (MQ=255)
                                                                     gATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGa                                                                           <  2:3147936/148‑1 (MQ=255)
                                                                     gATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGa                                                                           <  2:2122562/148‑1 (MQ=255)
                                                                      aTTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGAt                                                                          <  1:2941374/148‑1 (MQ=255)
                                                                       ttGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATCACGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATg                                                                         <  2:1965359/148‑1 (MQ=255)
                                                                       ttGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATg                                                                         >  2:1932209/1‑148 (MQ=255)
                                                                        tGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGc                                                                        >  2:391385/1‑148 (MQ=255)
                                                                            tatTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTg                                                                    <  1:2310664/148‑1 (MQ=255)
                                                                               tGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCg                                                                 >  1:2745335/1‑148 (MQ=255)
                                                                                gAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATCTGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCgg                                                                <  2:1482678/148‑1 (MQ=255)
                                                                                 aaGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGt                                                               >  1:2538318/1‑148 (MQ=255)
                                                                                      cAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAgg                                                          >  1:3330133/1‑148 (MQ=255)
                                                                                        ggTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAgggg                                                        <  1:1045115/148‑1 (MQ=255)
                                                                                        ggTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAgggg                                                        >  1:2359396/1‑148 (MQ=255)
                                                                                         gTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGc                                                       >  1:437631/1‑148 (MQ=255)
                                                                                             gTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGa                                                   <  2:2212176/148‑1 (MQ=255)
                                                                                               gCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATg                                                 <  1:2710601/148‑1 (MQ=255)
                                                                                                   aaCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcggc                                             >  1:2093866/1‑148 (MQ=255)
                                                                                                     cGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATTGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGa                                           <  2:1755597/148‑1 (MQ=255)
                                                                                                     cGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGa                                           <  2:3157300/148‑1 (MQ=255)
                                                                                                                 cGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTgcgc                               <  2:3517003/148‑1 (MQ=255)
                                                                                                                   tCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAg                             <  1:3425859/148‑1 (MQ=255)
                                                                                                                   tCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAg                             <  1:3426210/148‑1 (MQ=255)
                                                                                                                   tCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAg                             <  1:3426282/148‑1 (MQ=255)
                                                                                                                       aaTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCt                         >  1:978239/1‑148 (MQ=255)
                                                                                                                        aTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTa                        >  2:1244581/1‑148 (MQ=255)
                                                                                                                        aTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTa                        <  2:188771/148‑1 (MQ=255)
                                                                                                                         tAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTAt                       >  2:1557567/1‑148 (MQ=255)
                                                                                                                         tAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTAt                       >  1:3032626/1‑148 (MQ=255)
                                                                                                                          aaaGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATg                      <  1:939746/148‑1 (MQ=255)
                                                                                                                           aaGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGt                     <  1:287347/148‑1 (MQ=255)
                                                                                                                           aaGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGt                     <  1:3060313/148‑1 (MQ=255)
                                                                                                                             gCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTg                   >  2:2098930/1‑148 (MQ=255)
                                                                                                                             gCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTg                   >  2:362835/1‑148 (MQ=255)
                                                                                                                             gCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTg                   <  2:1898552/148‑1 (MQ=255)
                                                                                                                              cGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGa                  <  1:837638/148‑1 (MQ=255)
                                                                                                                              cGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGa                  <  1:137752/148‑1 (MQ=255)
                                                                                                                               gATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGaa                 <  2:1232448/148‑1 (MQ=255)
                                                                                                                                aTCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAg                >  2:3060647/1‑148 (MQ=255)
                                                                                                                                aTCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAg                >  1:234663/1‑148 (MQ=255)
                                                                                                                                 tCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGc               >  2:1393441/1‑148 (MQ=255)
                                                                                                                                 tCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGc               >  2:2942652/1‑148 (MQ=255)
                                                                                                                                 tCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGc               >  1:894652/1‑148 (MQ=255)
                                                                                                                                 tCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGc               >  1:1939753/1‑148 (MQ=255)
                                                                                                                                    tctcGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCggg            >  2:2306094/1‑148 (MQ=255)
                                                                                                                                    tctcGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCggg            >  2:451364/1‑148 (MQ=255)
                                                                                                                                      tcGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTg          >  2:295438/1‑148 (MQ=255)
                                                                                                                                           agaagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag     >  1:1463924/1‑148 (MQ=255)
                                                                                                                                           agaagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag     <  1:3118855/148‑1 (MQ=255)
                                                                                                                                           agaagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag     <  2:2149796/148‑1 (MQ=255)
                                                                                                                                             aagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAt   >  1:1987508/1‑148 (MQ=255)
                                                                                                                                              agatATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAtg  >  2:3190293/1‑148 (MQ=255)
                                                                                                                                              agatATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAtg  >  2:2103270/1‑148 (MQ=255)
                                                                                                                                              agatATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAtg  <  1:1076367/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATG  >  CP009273/344655‑344944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑