Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,337,927 A→T 44.9% V212E (GTA→GAA)  dcuB ← C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,337,9270AT44.9% 87.9 / inf 252V212E (GTA→GAA) dcuBC4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
Reads supporting (aligned to +/- strand):  ref base A (65/74);  new base T (50/63);  total (115/137)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 255 total aligned reads displayed.

GATGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAGG  >  CP009273/4337780‑4338074
                                                                                                                                                   |                                                                                                                                                   
gATGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTa                                                                                                                                                     >  2:493218/1‑148 (MQ=255)
     aCGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGgag                                                                                                                                                <  1:383753/148‑1 (MQ=255)
      cGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGgaga                                                                                                                                               >  2:362057/1‑148 (MQ=255)
      cGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGgaga                                                                                                                                               >  2:2154825/1‑148 (MQ=255)
      cGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGgaga                                                                                                                                               <  1:3547435/148‑1 (MQ=255)
          ggTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGaa                                                                                                                                           <  1:974753/148‑1 (MQ=255)
          ggTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGaa                                                                                                                                           >  1:3528180/1‑148 (MQ=255)
                   cAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGaa                                                                                                                                  <  1:752521/148‑1 (MQ=255)
                     gCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAAct                                                                                                                                >  2:680407/1‑148 (MQ=255)
                      cACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAActc                                                                                                                               >  1:3073909/1‑148 (MQ=255)
                      cACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAActc                                                                                                                               >  2:2322521/1‑148 (MQ=255)
                      cACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAActc                                                                                                                               >  2:2414934/1‑148 (MQ=255)
                        ccAAGAAGGGCGACTACAGCGAGTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCtt                                                                                                                             <  1:3482224/148‑1 (MQ=255)
                         cAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTc                                                                                                                            >  2:1585939/1‑148 (MQ=255)
                         cAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCAAAATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTc                                                                                                                            >  2:297133/1‑148 (MQ=255)
                          aagaagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCg                                                                                                                           <  1:2161300/148‑1 (MQ=255)
                          aagaagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCg                                                                                                                           <  1:1988532/148‑1 (MQ=255)
                           agaagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGt                                                                                                                          <  2:392546/148‑1 (MQ=255)
                           agaagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGt                                                                                                                          >  1:2359073/1‑148 (MQ=255)
                             aagGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCt                                                                                                                        >  2:66489/1‑148 (MQ=255)
                               gggCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCttt                                                                                                                      >  2:2535614/1‑148 (MQ=255)
                                   gACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATcc                                                                                                                  <  1:3289258/148‑1 (MQ=255)
                                      tACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGa                                                                                                               <  1:3024203/148‑1 (MQ=255)
                                        cAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATc                                                                                                             <  1:1725583/148‑1 (MQ=255)
                                         aGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCt                                                                                                            <  1:2721712/148‑1 (MQ=255)
                                          gCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCtt                                                                                                           <  1:1699499/148‑1 (MQ=255)
                                           cGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCttt                                                                                                          <  2:2469960/148‑1 (MQ=255)
                                            gATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTa                                                                                                         <  1:3351605/148‑1 (MQ=255)
                                             aTTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTAc                                                                                                        >  2:3452618/1‑148 (MQ=255)
                                             aTTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTAc                                                                                                        <  2:1404392/148‑1 (MQ=255)
                                             aTTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTAc                                                                                                        <  2:3176442/148‑1 (MQ=255)
                                                 ccccGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCAAGATCTTTACagcg                                                                                                    >  2:1854369/1‑148 (MQ=255)
                                                   ccGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGa                                                                                                  >  1:2694800/1‑148 (MQ=255)
                                                     gAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAAc                                                                                                >  1:3464485/1‑148 (MQ=255)
                                                      aGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGGGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAAcc                                                                                               <  1:549934/148‑1 (MQ=255)
                                                        gAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAg                                                                                             >  1:249039/1‑148 (MQ=255)
                                                        gAAAATCCACATTGCCAGCAAGTTGCTTTTAGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTAACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACAGCGTAACCAg                                                                                             >  2:3481157/1‑148 (MQ=255)
                                                         aaaaTCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGc                                                                                            >  2:2375539/1‑148 (MQ=255)
                                                           aaTCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTg                                                                                          >  2:835698/1‑148 (MQ=255)
                                                            aTCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGa                                                                                         >  2:78984/1‑148 (MQ=255)
                                                            aTCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGa                                                                                         >  2:2791578/1‑148 (MQ=255)
                                                               cacaTTGCCAGCCAGTTGCTTTTCGGGAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGa                                                                                      <  2:3205803/148‑1 (MQ=255)
                                                               cacaTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGa                                                                                      <  2:3516601/148‑1 (MQ=255)
                                                                acaTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGAt                                                                                     >  2:556139/1‑148 (MQ=255)
                                                                acaTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATAATCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGAt                                                                                     >  1:1273610/1‑148 (MQ=255)
                                                                 caTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATa                                                                                    >  1:1610914/1‑148 (MQ=255)
                                                                  aTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATAc                                                                                   <  2:1714997/148‑1 (MQ=255)
                                                                  aTTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATAc                                                                                   >  1:1010381/1‑148 (MQ=255)
                                                                    tGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCg                                                                                 <  1:625972/148‑1 (MQ=255)
                                                                    tGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCg                                                                                 >  2:1177516/1‑148 (MQ=255)
                                                                     gccagccaGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGa                                                                                <  2:1931556/148‑1 (MQ=255)
                                                                     gccagccaGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGa                                                                                <  2:2974943/148‑1 (MQ=255)
                                                                      ccagccagTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGAt                                                                               <  1:889118/148‑1 (MQ=255)
                                                                         gccagTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGc                                                                            <  1:2079155/148‑1 (MQ=255)
                                                                          ccagTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCATATCTTTACCGCGGAACCAGCTGAAGATACCGATCGcc                                                                           >  1:2423790/1‑148 (MQ=255)
                                                                                gCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATa                                                                     <  1:2535575/148‑1 (MQ=255)
                                                                                  ttttCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATAcc                                                                   <  1:493218/148‑1 (MQ=255)
                                                                                     tCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGAt                                                                <  2:2947471/148‑1 (MQ=255)
                                                                                        gCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTaa                                                             >  1:1879168/1‑148 (MQ=255)
                                                                                         cAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAAc                                                            >  2:1201236/1‑148 (MQ=255)
                                                                                           gTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACga                                                          >  1:2604544/1‑147 (MQ=255)
                                                                                             tttttATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCg                                                        <  2:327568/148‑1 (MQ=255)
                                                                                             tttttATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCg                                                        <  2:325248/148‑1 (MQ=255)
                                                                                              ttttATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGa                                                       >  1:2707172/1‑148 (MQ=255)
                                                                                               tttATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGAt                                                      <  1:1468286/148‑1 (MQ=255)
                                                                                                ttATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATg                                                     >  2:934724/1‑148 (MQ=255)
                                                                                                   tCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGaa                                                  <  1:204368/148‑1 (MQ=255)
                                                                                                   tCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCAGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGaa                                                  <  2:2601662/148‑1 (MQ=255)
                                                                                                        cagcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTg                                             <  1:2375539/148‑1 (MQ=255)
                                                                                                        cagcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTg                                             <  1:2962363/148‑1 (MQ=255)
                                                                                                        cagcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTg                                             <  2:1987625/148‑1 (MQ=255)
                                                                                                        cagcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTg                                             >  1:535296/1‑148 (MQ=255)
                                                                                                         agcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGa                                            <  2:1434951/148‑1 (MQ=255)
                                                                                                          gcGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGAt                                           <  1:447448/148‑1 (MQ=255)
                                                                                                           cGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGAtt                                          >  2:179608/1‑148 (MQ=255)
                                                                                                              cgcgGTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGcc                                       >  1:3207857/1‑148 (MQ=255)
                                                                                                              cgcgGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGcc                                       >  2:192284/1‑148 (MQ=255)
                                                                                                                 ggTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCcagc                                    >  2:3094994/1‑148 (MQ=255)
                                                                                                                 ggTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCcagc                                    <  1:1040203/148‑1 (MQ=255)
                                                                                                                  gTATCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCcagca                                   >  2:3315807/1‑148 (MQ=255)
                                                                                                                    aTCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGa                                 <  2:2643971/148‑1 (MQ=255)
                                                                                                                     tCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGAt                                >  1:1817251/1‑148 (MQ=255)
                                                                                                                     tCACCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGAt                                <  2:3073909/148‑1 (MQ=255)
                                                                                                                       aCCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCg                              <  1:1410805/148‑1 (MQ=255)
                                                                                                                       aCCGTAAACATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCg                              <  1:2283043/148‑1 (MQ=255)
                                                                                                                            aaaCATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGaa                         >  2:3499900/1‑148 (MQ=255)
                                                                                                                             aaCATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAAc                        >  2:468480/1‑148 (MQ=255)
                                                                                                                             aaCATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAAc                        >  2:1157669/1‑148 (MQ=255)
                                                                                                                             aaCATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAAc                        >  2:1157558/1‑148 (MQ=255)
                                                                                                                              aCATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACt                       >  1:3040208/1‑148 (MQ=255)
                                                                                                                              aCATACTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACt                       >  1:1256550/1‑148 (MQ=255)
                                                                                                                                  aCTCACGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAg                   <  1:221392/148‑1 (MQ=255)
                                                                                                                                  aCTCACGGTTTTCCGGTACGTAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATAACGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAg                   <  1:2010048/148‑1 (MQ=255)
                                                                                                                                      aCGGTTTTCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATgg               <  2:1072164/148‑1 (MQ=255)
                                                                                                                                            ttCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCa         <  2:1273607/148‑1 (MQ=255)
                                                                                                                                            ttCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCa         <  2:1273627/148‑1 (MQ=255)
                                                                                                                                             tCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCAt        >  1:1135606/1‑148 (MQ=255)
                                                                                                                                             tCCGGTTCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCAt        >  1:1135566/1‑148 (MQ=255)
                                                                                                                                              ccGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATc       <  2:1845899/148‑1 (MQ=255)
                                                                                                                                                   aCGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAgg  <  1:2740173/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATGGACGCAGGTCCGAATCAGCACCAAGAAGGGCGACTACAGCGATTGCCCCGAGGAAAATCCACATTGCCAGCCAGTTGCTTTTCGGCAGTTTTTTATCCAGCAGCGTCGCGGTATCACCGTAAACATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAGG  >  CP009273/4337780‑4338074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑