Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,366,322 A→C 100% intergenic (+63/‑48) ecnA → / → ecnB entericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,366,3220AC96.3% 934.4 / 8.2 238intergenic (+63/‑48)ecnA/ecnBentericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (8/1);  new base C (131/98);  total (139/99)
Fisher's exact test for biased strand distribution p-value = 8.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

TGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTG  >  CP009273/4366177‑4366469
                                                                                                                                                 |                                                                                                                                                   
    cACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGc                                                                                                                                               >  2:3304405/1‑148 (MQ=255)
    cACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGc                                                                                                                                               >  1:1346190/1‑148 (MQ=255)
    cACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATATCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGc                                                                                                                                               <  1:2370083/148‑1 (MQ=255)
      cGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCta                                                                                                                                             >  2:699444/1‑148 (MQ=255)
            gTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCa                                                                                                                                       <  2:2449484/148‑1 (MQ=255)
             tAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCaa                                                                                                                                      >  2:526829/1‑148 (MQ=255)
                  ccGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACata                                                                                                                                 >  1:3375898/1‑148 (MQ=255)
                     cTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataaat                                                                                                                              >  1:749608/1‑148 (MQ=255)
                         cgGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACa                                                                                                                          <  2:811766/148‑1 (MQ=255)
                            tttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTa                                                                                                                       <  2:2614429/148‑1 (MQ=255)
                            tttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTa                                                                                                                       <  2:3336876/148‑1 (MQ=255)
                             ttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTAc                                                                                                                      >  1:461586/1‑148 (MQ=255)
                                    aaGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAg                                                                                                               <  1:3296560/148‑1 (MQ=255)
                                     aGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAgg                                                                                                              <  1:1647133/148‑1 (MQ=255)
                                     aGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAgg                                                                                                              <  1:2021609/148‑1 (MQ=255)
                                        cATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAg                                                                                                           >  1:924224/1‑148 (MQ=255)
                                                 tctcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATgg                                                                                                  >  2:369274/1‑148 (MQ=255)
                                                 tctcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATgg                                                                                                  >  2:1176567/1‑148 (MQ=255)
                                                 tctcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATgg                                                                                                  >  2:437428/1‑148 (MQ=255)
                                                    cGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTga                                                                                               >  1:945321/1‑148 (MQ=255)
                                                     ggCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaa                                                                                              <  2:2460025/148‑1 (MQ=255)
                                                      gCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaag                                                                                             <  1:2063654/148‑1 (MQ=255)
                                                        aaCTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaagaa                                                                                           <  2:2806174/148‑1 (MQ=255)
                                                          cTCAATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTAGGGTGAagaaga                                                                                         >  2:1501536/1‑148 (MQ=255)
                                                           tCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGAc                                                                                        <  1:2173871/148‑1 (MQ=255)
                                                           tCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGAc                                                                                        >  2:1904587/1‑148 (MQ=255)
                                                           tCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGAc                                                                                        >  2:1904605/1‑148 (MQ=255)
                                                            ccATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACa                                                                                       <  2:1660145/148‑1 (MQ=255)
                                                                  ctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCa                                                                                 <  2:3294251/148‑1 (MQ=255)
                                                                    cgcgCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGc                                                                               >  1:1313438/1‑148 (MQ=255)
                                                                    cgcgCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTAGGGTGAAGAAGACAATTGCAGc                                                                               >  2:1846592/1‑148 (MQ=255)
                                                                     gcgcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCg                                                                              <  1:983201/148‑1 (MQ=255)
                                                                     gcgcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCg                                                                              <  1:699444/148‑1 (MQ=255)
                                                                      cgcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGa                                                                             >  2:1119286/1‑148 (MQ=255)
                                                                       gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGAt                                                                            <  2:569822/148‑1 (MQ=255)
                                                                         tGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCt                                                                          <  1:2938977/148‑1 (MQ=255)
                                                                         tGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCt                                                                          >  1:1453607/1‑148 (MQ=255)
                                                                          gCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCtt                                                                         >  2:2686297/1‑148 (MQ=255)
                                                                           ccAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCttt                                                                        <  1:521095/148‑1 (MQ=255)
                                                                            cAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCtttt                                                                       >  1:1108584/1‑148 (MQ=255)
                                                                            cAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCtttt                                                                       <  2:281488/148‑1 (MQ=255)
                                                                             aGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttc                                                                      >  1:1713871/1‑148 (MQ=255)
                                                                             aGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttc                                                                      >  1:2823437/1‑148 (MQ=255)
                                                                              gCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttct                                                                     >  2:942777/1‑148 (MQ=255)
                                                                               cTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctg                                                                    >  2:821514/1‑148 (MQ=255)
                                                                                tAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgt                                                                   <  2:3375898/148‑1 (MQ=255)
                                                                                tAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgt                                                                   >  1:2033706/1‑148 (MQ=255)
                                                                                 aaTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGAGGTTATGGTGAAGAAGACAATTGCAGCGATCTTtgctgtt                                                                  >  1:2161740/1‑148 (MQ=255)
                                                                                 aaTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgtt                                                                  <  1:2515791/148‑1 (MQ=255)
                                                                                  aTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgttc                                                                 <  1:127460/148‑1 (MQ=255)
                                                                                    ttttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgttctg                                                               <  1:955427/148‑1 (MQ=255)
                                                                                     tttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTgg                                                              >  1:3443572/1‑148 (MQ=255)
                                                                                      ttcttcTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAATGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGt                                                             >  1:2587773/1‑148 (MQ=255)
                                                                                       tcttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTg                                                            <  1:497248/148‑1 (MQ=255)
                                                                                         ttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCt                                                          <  1:1190465/148‑1 (MQ=255)
                                                                                             cttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCa                                                      >  1:2731828/1‑148 (MQ=255)
                                                                                                   aaaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTa                                                >  1:2117633/1‑148 (MQ=255)
                                                                                                   aaaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTa                                                <  2:1331575/148‑1 (MQ=255)
                                                                                                    aaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAt                                               >  2:2039034/1‑148 (MQ=255)
                                                                                                    aaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAt                                               >  2:2038404/1‑148 (MQ=255)
                                                                                                     aaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAtt                                              >  2:1596583/1‑148 (MQ=255)
                                                                                                       atcatcAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTaa                                            >  2:2524436/1‑148 (MQ=255)
                                                                                                       atcatcAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTaa                                            >  2:2524594/1‑148 (MQ=255)
                                                                                                       atcatcAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTaa                                            >  2:3089733/1‑148 (MQ=255)
                                                                                                         catcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAAct                                          >  2:2249104/1‑148 (MQ=255)
                                                                                                         catcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAAct                                          >  2:2564163/1‑148 (MQ=255)
                                                                                                         catcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAAct                                          >  2:1407840/1‑148 (MQ=255)
                                                                                                             aGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAActgcct                                      >  2:280639/1‑148 (MQ=255)
                                                                                                               aTTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAActgcctgc                                    >  2:1698862/1‑148 (MQ=255)
                                                                                                                gtCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCa                                   <  2:2120091/147‑1 (MQ=255)
                                                                                                                 tCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCaa                                  >  2:2699846/1‑148 (MQ=255)
                                                                                                                 tCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCaa                                  >  2:3235540/1‑148 (MQ=255)
                                                                                                                 tCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCaa                                  >  2:1758214/1‑148 (MQ=255)
                                                                                                                  cccATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAac                                 >  1:3016991/1‑148 (MQ=255)
                                                                                                                   ccATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAaca                                >  2:2852342/1‑148 (MQ=255)
                                                                                                                   ccATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAaca                                >  2:1072009/1‑148 (MQ=255)
                                                                                                                    catcatTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAacac                               <  1:100697/148‑1 (MQ=255)
                                                                                                                      tcatTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAAcacca                             <  1:1949002/148‑1 (MQ=255)
                                                                                                                        atTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcg                           >  1:2686712/1‑148 (MQ=255)
                                                                                                                          ttttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcgcg                         <  1:60933/148‑1 (MQ=255)
                                                                                                                          ttttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcgcg                         <  1:1866116/148‑1 (MQ=255)
                                                                                                                           tttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGt                        >  1:876246/1‑148 (MQ=255)
                                                                                                                             tGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTgg                      <  2:292320/148‑1 (MQ=255)
                                                                                                                               gCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCg                    <  1:310002/148‑1 (MQ=255)
                                                                                                                                cGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGt                   >  2:2231355/1‑148 (MQ=255)
                                                                                                                                  aTGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg                 >  2:2639854/1‑148 (MQ=255)
                                                                                                                                  aTGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg                 <  1:1176505/148‑1 (MQ=255)
                                                                                                                                  aTGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg                 >  1:3522685/1‑148 (MQ=255)
                                                                                                                                     ttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTg              >  1:1289100/1‑148 (MQ=255)
                                                                                                                                       gtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa            <  1:2367582/148‑1 (MQ=255)
                                                                                                                                          tattatTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGAc         <  1:1904604/148‑1 (MQ=255)
                                                                                                                                           attattCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCCGCAACACCACGCGTGGCGTTGGTGAAGACa        <  2:1346161/148‑1 (MQ=255)
                                                                                                                                            ttattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAt       <  1:2301551/148‑1 (MQ=255)
                                                                                                                                            ttattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAt       <  1:2639777/148‑1 (MQ=255)
                                                                                                                                            ttattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAt       <  1:2277602/148‑1 (MQ=255)
                                                                                                                                             tattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACagt      >  2:3004318/1‑146 (MQ=255)
                                                                                                                                             tattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAtt      <  2:2355006/148‑1 (MQ=255)
                                                                                                                                              attcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt     <  1:1322263/148‑1 (MQ=255)
                                                                                                                                              attcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt     >  2:635068/1‑148 (MQ=255)
                                                                                                                                              attaATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACACTTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt     <  2:3407814/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTG  >  CP009273/4366177‑4366469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑