Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,376,178 C→A 100% E1004* (GAA→TAA)  mscM ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,376,1780CA96.7% 552.3 / 9.1 184E1004* (GAA→TAA) mscMmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (99/79);  total (102/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 184 total aligned reads displayed.

CGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGG  >  CP009273/4376033‑4376318
                                                                                                                                                 |                                                                                                                                            
 ggTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCt                                                                                                                                           <  2:2729805/148‑1 (MQ=255)
  gTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTa                                                                                                                                          <  1:3043371/148‑1 (MQ=255)
  gTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTAGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTa                                                                                                                                          <  1:1254960/148‑1 (MQ=255)
    gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTAtt                                                                                                                                        <  2:3057135/148‑1 (MQ=255)
       ccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGc                                                                                                                                     <  2:1292037/148‑1 (MQ=255)
        cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCa                                                                                                                                    <  1:1677130/148‑1 (MQ=255)
             cAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGc                                                                                                                               >  2:2228959/1‑148 (MQ=255)
             cAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGc                                                                                                                               >  2:2991867/1‑148 (MQ=255)
              aGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCa                                                                                                                              <  2:2631169/148‑1 (MQ=255)
               gcggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAg                                                                                                                             <  1:410212/148‑1 (MQ=255)
                cggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgg                                                                                                                            >  2:698307/1‑148 (MQ=255)
                cggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgg                                                                                                                            >  2:2386570/1‑148 (MQ=255)
                cggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgg                                                                                                                            <  1:2431057/148‑1 (MQ=255)
                  gcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgggg                                                                                                                          >  2:844674/1‑148 (MQ=255)
                  gcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgggg                                                                                                                          >  2:878777/1‑148 (MQ=255)
                    gTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGcc                                                                                                                        >  2:706262/1‑148 (MQ=255)
                    gTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGcc                                                                                                                        >  2:2083319/1‑148 (MQ=255)
                     tAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCg                                                                                                                       >  2:687273/1‑148 (MQ=255)
                      aaaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCgg                                                                                                                      >  1:110959/1‑148 (MQ=255)
                      aaaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCgg                                                                                                                      >  2:769601/1‑148 (MQ=255)
                         tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAt                                                                                                                   >  1:135686/1‑148 (MQ=255)
                         tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAt                                                                                                                   >  1:365810/1‑148 (MQ=255)
                         tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAt                                                                                                                   >  1:909330/1‑148 (MQ=255)
                         tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAt                                                                                                                   >  1:2064167/1‑148 (MQ=255)
                         tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGAGCCGGTAt                                                                                                                   >  1:909358/1‑148 (MQ=255)
                          aCGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATc                                                                                                                  >  2:640568/1‑148 (MQ=255)
                          aCGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATc                                                                                                                  >  2:640513/1‑148 (MQ=255)
                             cAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTCTCGTc                                                                                                               >  1:463586/1‑148 (MQ=255)
                              aGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCa                                                                                                              <  1:3037181/148‑1 (MQ=255)
                                   gAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcacc                                                                                                         >  2:292363/1‑148 (MQ=255)
                                     aaaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcaccac                                                                                                       >  2:1487935/1‑148 (MQ=255)
                                      aaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcg                                                                                                      >  2:2277984/1‑148 (MQ=255)
                                       aTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgc                                                                                                     >  1:1396870/1‑148 (MQ=255)
                                        tCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcg                                                                                                    >  2:933457/1‑148 (MQ=255)
                                         cTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcgc                                                                                                   >  1:2176187/1‑148 (MQ=255)
                                            aaTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTg                                                                                                >  2:3048641/1‑148 (MQ=255)
                                             aTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCCGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGa                                                                                               <  1:2637720/148‑1 (MQ=255)
                                             aTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGa                                                                                               >  2:667297/1‑148 (MQ=255)
                                              tCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAc                                                                                              >  1:2744543/1‑148 (MQ=255)
                                               ccccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAcc                                                                                             >  2:2764011/1‑148 (MQ=255)
                                                cccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                            >  2:1166772/1‑148 (MQ=255)
                                                  cTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAg                                                                                          >  2:3027434/1‑148 (MQ=255)
                                                    gTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTc                                                                                        <  2:3339381/148‑1 (MQ=255)
                                                         aGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagaga                                                                                   <  2:1565591/148‑1 (MQ=255)
                                                          gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagagag                                                                                  <  1:359674/148‑1 (MQ=255)
                                                          gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagagag                                                                                  >  2:3039480/1‑148 (MQ=255)
                                                            tCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCg                                                                                <  1:3040968/148‑1 (MQ=255)
                                                              caccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAc                                                                              <  2:664054/148‑1 (MQ=255)
                                                                   ggAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt                                                                         >  1:2945000/1‑148 (MQ=255)
                                                                   ggAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt                                                                         >  1:2014840/1‑148 (MQ=255)
                                                                    taaGACTTCCGGTGCGGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                        <  1:2790312/147‑1 (MQ=255)
                                                                    gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                        <  1:1008451/148‑1 (MQ=255)
                                                                    gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                        >  2:3240531/1‑148 (MQ=255)
                                                                    gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                        >  1:1661576/1‑148 (MQ=255)
                                                                     aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGa                                                                       >  1:2697073/1‑148 (MQ=255)
                                                                       gACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTCTTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATa                                                                     >  2:2547033/1‑148 (MQ=255)
                                                                       gACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATa                                                                     <  2:214379/148‑1 (MQ=255)
                                                                        aCTTCCGGTGCCGGTTTTTCGATCACCAGCGTGCAGCGAGGCGCTGCGGTGAGCAGTATTTCCGTAACTTCTTAGCTATTGGCTTCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGTGTCAGAGAGCGACCAGTTGATaa                                                                    <  1:131378/148‑1 (MQ=255)
                                                                         cTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATaaa                                                                   <  1:3147312/148‑1 (MQ=255)
                                                                              ggTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCt                                                              <  2:202375/148‑1 (MQ=255)
                                                                                tGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCg                                                            <  2:1601138/148‑1 (MQ=255)
                                                                                  ccGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGt                                                          >  2:306085/1‑148 (MQ=255)
                                                                                   cGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGta                                                         <  2:2648293/148‑1 (MQ=255)
                                                                                   cGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGta                                                         <  2:2759792/148‑1 (MQ=255)
                                                                                    gggTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaa                                                        <  2:2433794/148‑1 (MQ=255)
                                                                                       ttGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaataa                                                     <  1:3444522/148‑1 (MQ=255)
                                                                                        tGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATaaa                                                    <  1:2382666/148‑1 (MQ=255)
                                                                                        tGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATaaa                                                    <  1:2382565/148‑1 (MQ=255)
                                                                                        tGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATaaa                                                    <  2:807077/148‑1 (MQ=255)
                                                                                            gATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGcc                                                <  1:2805162/148‑1 (MQ=255)
                                                                                                  cAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTc                                          >  1:1937121/1‑148 (MQ=255)
                                                                                                  cAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTc                                          >  2:3392471/1‑148 (MQ=255)
                                                                                                      gAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCa                                      <  2:3558907/148‑1 (MQ=255)
                                                                                                         cagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGa                                   <  1:1699189/148‑1 (MQ=255)
                                                                                                          agcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAt                                  <  2:1530060/148‑1 (MQ=255)
                                                                                                          agcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAt                                  <  2:1529507/148‑1 (MQ=255)
                                                                                                            cGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                >  2:969878/1‑148 (MQ=255)
                                                                                                            cGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                <  1:1033379/148‑1 (MQ=255)
                                                                                                            cGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                <  2:2666964/148‑1 (MQ=255)
                                                                                                            cGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTa                                <  1:130715/148‑1 (MQ=255)
                                                                                                             gACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAt                               <  2:3426215/148‑1 (MQ=255)
                                                                                                             gACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAt                               <  1:687273/148‑1 (MQ=255)
                                                                                                               cgcgCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtct                             <  1:2472067/148‑1 (MQ=255)
                                                                                                                gcgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtctc                            <  1:1487935/148‑1 (MQ=255)
                                                                                                                 cgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATctct                           >  2:307239/1‑148 (MQ=255)
                                                                                                                  gcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCtt                          <  1:3527595/148‑1 (MQ=255)
                                                                                                                   cTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCttt                         >  2:1788434/1‑148 (MQ=255)
                                                                                                                          gAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTcc                  >  1:715046/1‑148 (MQ=255)
                                                                                                                             cAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAg               >  2:3463886/1‑148 (MQ=255)
                                                                                                                             cAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAg               >  2:3522793/1‑148 (MQ=255)
                                                                                                                                gATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCg            >  2:3159019/1‑148 (MQ=255)
                                                                                                                                   ttCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg         <  2:1033921/148‑1 (MQ=255)
                                                                                                                                   ttCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg         <  2:1663450/148‑1 (MQ=255)
                                                                                                                                   ttCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg         >  1:3278363/1‑148 (MQ=255)
                                                                                                                                   ttCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg         >  1:1689524/1‑148 (MQ=255)
                                                                                                                                    tCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGa        >  2:134296/1‑148 (MQ=255)
                                                                                                                                     ccGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAt       <  1:3039494/148‑1 (MQ=255)
                                                                                                                                       gTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtgg     >  1:1632543/1‑148 (MQ=255)
                                                                                                                                        tCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggt    >  2:1025267/1‑148 (MQ=255)
                                                                                                                                        tCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggt    >  2:3172372/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGG  >  CP009273/4376033‑4376318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑