Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,381,504 T→A 100% I28I (ATT→ATA)  orn → oligoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,381,5040TA99.5% 693.8 / ‑4.6 209I28I (ATT→ATA) ornoligoribonuclease
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (113/95);  total (114/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

AAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAA  >  CP009273/4381359‑4381647
                                                                                                                                                 |                                                                                                                                               
aaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGc                                                                                                                                               <  2:2051305/148‑1 (MQ=255)
aaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGc                                                                                                                                               <  2:2932220/148‑1 (MQ=255)
 aaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGcc                                                                                                                                              <  2:1962844/148‑1 (MQ=255)
  aCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCa                                                                                                                                             <  1:3298958/148‑1 (MQ=255)
  aCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCa                                                                                                                                             <  2:1501064/148‑1 (MQ=255)
    aTCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACg                                                                                                                                           <  2:850815/148‑1 (MQ=255)
    aTCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACg                                                                                                                                           >  2:1406099/1‑148 (MQ=255)
      cTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCt                                                                                                                                         <  1:2015472/148‑1 (MQ=255)
           aTACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGa                                                                                                                                    >  1:2892782/1‑148 (MQ=255)
           aTACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGa                                                                                                                                    <  2:2111197/148‑1 (MQ=255)
            tACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGAGAGCCACGCTGGTGAc                                                                                                                                   <  2:2327483/148‑1 (MQ=255)
               acGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGa                                                                                                                                >  1:953858/1‑148 (MQ=255)
                cGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGAt                                                                                                                               <  1:408624/148‑1 (MQ=255)
                  cAATTGTGTATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGAATTATTGAGATAGCCACGCTGGTGACCGATGc                                                                                                                             <  1:3378480/148‑1 (MQ=255)
                  cAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGc                                                                                                                             <  1:1722379/148‑1 (MQ=255)
                  cAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGc                                                                                                                             <  1:1176579/148‑1 (MQ=255)
                    aTTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCa                                                                                                                           <  2:1085541/148‑1 (MQ=255)
                     ttGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCaa                                                                                                                          >  2:2852862/1‑148 (MQ=255)
                      tgtgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAAc                                                                                                                         >  1:2928153/1‑148 (MQ=255)
                       gtgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAAcc                                                                                                                        <  1:44182/148‑1 (MQ=255)
                             cAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCAATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAata                                                                                                                  >  2:815031/1‑148 (MQ=255)
                               atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATAtt                                                                                                                >  1:2288622/1‑148 (MQ=255)
                               atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATAtt                                                                                                                <  2:1567895/148‑1 (MQ=255)
                                tataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                               >  2:363133/1‑148 (MQ=255)
                                tataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                               >  2:3482770/1‑148 (MQ=255)
                                tataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                               >  2:1265024/1‑148 (MQ=255)
                                 ataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCt                                                                                                              >  1:3451245/1‑148 (MQ=255)
                                   aGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTgg                                                                                                            <  2:3434819/148‑1 (MQ=255)
                                   aGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTgg                                                                                                            <  2:393241/148‑1 (MQ=255)
                                   aGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTgg                                                                                                            >  1:2463205/1‑148 (MQ=255)
                                   aGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTgg                                                                                                            <  1:1464654/148‑1 (MQ=255)
                                     tCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCa                                                                                                          >  2:3181112/1‑148 (MQ=255)
                                       aCTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGa                                                                                                        <  1:2201138/148‑1 (MQ=255)
                                         tgtgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAg                                                                                                      >  1:2995029/1‑148 (MQ=255)
                                            gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGc                                                                                                   >  2:247786/1‑148 (MQ=255)
                                               tGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGa                                                                                                >  2:1590003/1‑148 (MQ=255)
                                                gggTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGAc                                                                                               >  1:3157883/1‑148 (MQ=255)
                                                 ggTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGAcc                                                                                              <  1:1159869/148‑1 (MQ=255)
                                                  gTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCa                                                                                             <  2:1583765/148‑1 (MQ=255)
                                                   tGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAt                                                                                            <  1:778442/148‑1 (MQ=255)
                                                     gAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTg                                                                                          >  1:1303526/1‑148 (MQ=255)
                                                       caagaGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCGTGAGATGACCGGTCTGGATCCCGAGCGCGCTCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCa                                                                                        <  2:615090/144‑1 (MQ=255)
                                                        aaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAg                                                                                       >  1:2565504/1‑148 (MQ=255)
                                                          tAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTa                                                                                     >  1:1485584/1‑148 (MQ=255)
                                                               tGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCa                                                                                <  2:2013213/148‑1 (MQ=255)
                                                                gAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAg                                                                               >  2:2738772/1‑148 (MQ=255)
                                                                gAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAg                                                                               >  2:959930/1‑148 (MQ=255)
                                                                 aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGt                                                                              >  2:1003546/1‑148 (MQ=255)
                                                                 aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGt                                                                              >  2:249281/1‑148 (MQ=255)
                                                                  gTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTc                                                                             <  2:267657/148‑1 (MQ=255)
                                                                      cAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgat                                                                         <  2:2928153/148‑1 (MQ=255)
                                                                      cAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgat                                                                         >  1:1361914/1‑148 (MQ=255)
                                                                       aaTGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatg                                                                        >  1:686175/1‑148 (MQ=255)
                                                                       aaTGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatg                                                                        <  2:87335/148‑1 (MQ=255)
                                                                         tGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGaa                                                                      >  1:234448/1‑148 (MQ=255)
                                                                           aaaaCAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACa                                                                    >  1:3481104/1‑148 (MQ=255)
                                                                               caacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTg                                                                >  2:3504172/1‑148 (MQ=255)
                                                                               caacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTg                                                                <  2:1092257/148‑1 (MQ=255)
                                                                                   cTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGgcgc                                                            >  1:2618994/1‑148 (MQ=255)
                                                                                     gATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTg                                                          >  1:121404/1‑148 (MQ=255)
                                                                                      aTTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTga                                                         <  2:2381799/148‑1 (MQ=255)
                                                                                      aTTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGAAGTACACCAGTCTGATGAACAGCTGGCGCTga                                                         >  1:85201/1‑148 (MQ=255)
                                                                                         tGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatgg                                                      >  1:1576724/1‑148 (MQ=255)
                                                                                          ggATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatgga                                                     <  1:235786/148‑1 (MQ=255)
                                                                                             tcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGa                                                  <  2:3317474/148‑1 (MQ=255)
                                                                                                 tcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                              >  1:1915116/1‑148 (MQ=255)
                                                                                                  cTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGa                                             <  1:1072529/148‑1 (MQ=255)
                                                                                                   ttGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGaa                                            <  2:3062492/148‑1 (MQ=255)
                                                                                                    tGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAAc                                           >  1:1397850/1‑148 (MQ=255)
                                                                                                      agaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGt                                         <  2:716952/148‑1 (MQ=255)
                                                                                                       gaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTg                                        >  2:2430905/1‑148 (MQ=255)
                                                                                                          gACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgcgc                                     >  1:1966897/1‑148 (MQ=255)
                                                                                                              ggTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAccc                                 <  2:410580/148‑1 (MQ=255)
                                                                                                              ggTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAccc                                 <  2:3234506/148‑1 (MQ=255)
                                                                                                              ggTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAccc                                 <  2:923192/148‑1 (MQ=255)
                                                                                                                tCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCAt                               <  2:1843042/148‑1 (MQ=255)
                                                                                                                 cTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATa                              <  2:2239055/148‑1 (MQ=255)
                                                                                                                 cTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATa                              <  1:2035892/148‑1 (MQ=255)
                                                                                                                 cTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATa                              <  2:210007/148‑1 (MQ=255)
                                                                                                                   ggATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAcc                            >  1:572044/1‑148 (MQ=255)
                                                                                                                     aTCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGc                          >  2:2644367/1‑148 (MQ=255)
                                                                                                                      tCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGcc                         >  1:1885911/1‑148 (MQ=255)
                                                                                                                        ccGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAg                       >  2:506734/1‑148 (MQ=255)
                                                                                                                          gAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCg                     >  1:2604960/1‑148 (MQ=255)
                                                                                                                            gcgcGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                   >  2:1060137/1‑148 (MQ=255)
                                                                                                                            gcgcGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                   <  2:2311287/148‑1 (MQ=255)
                                                                                                                            gcgcGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                   >  2:1882200/1‑148 (MQ=255)
                                                                                                                            gcgcGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                   <  1:247922/148‑1 (MQ=255)
                                                                                                                             cgcgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGc                  <  1:891132/148‑1 (MQ=255)
                                                                                                                               cgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTg                >  1:952815/1‑148 (MQ=255)
                                                                                                                                gATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTgg               <  2:1458546/148‑1 (MQ=255)
                                                                                                                                  tCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTa             >  2:1744351/1‑148 (MQ=255)
                                                                                                                                  tCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTa             >  1:2724949/1‑148 (MQ=255)
                                                                                                                                   cGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTag            >  1:628450/1‑148 (MQ=255)
                                                                                                                                     cATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTagag          >  1:817857/1‑148 (MQ=255)
                                                                                                                                     cATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTagag          >  2:3357265/1‑148 (MQ=255)
                                                                                                                                         attGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGcgcg      >  2:1137221/1‑148 (MQ=255)
                                                                                                                                          ttGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGt     >  2:447338/1‑148 (MQ=255)
                                                                                                                                             agaTAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGaa  >  1:828170/1‑148 (MQ=255)
                                                                                                                                             agaTAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGAAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGaa  <  1:1264745/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAA  >  CP009273/4381359‑4381647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑