Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,411,145 T→G 9.6% L450R (CTG→CGG)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,411,1450TG 9.6% 327.0 / 3.5 182L450R (CTG→CGG) ulaAL‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base T (74/84);  new base G (18/5);  total (93/89)
Fisher's exact test for biased strand distribution p-value = 6.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

CACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAC  >  CP009273/4410999‑4411292
                                                                                                                                                  |                                                                                                                                                   
cACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTg                                                                                                                                                    <  2:2799800/148‑1 (MQ=255)
        tGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCaga                                                                                                                                            <  1:1666709/148‑1 (MQ=255)
         gAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATAGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaa                                                                                                                                           >  2:2364240/1‑148 (MQ=255)
             gCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaag                                                                                                                                       <  2:693453/148‑1 (MQ=255)
             gCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaag                                                                                                                                       <  1:41048/148‑1 (MQ=255)
              ccTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaaga                                                                                                                                      <  1:2254425/148‑1 (MQ=255)
              ccTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaagaaga                                                                                                                                      <  1:3528983/148‑1 (MQ=255)
              ccTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCcgaagaaga                                                                                                                                      >  1:181310/1‑148 (MQ=255)
                  gATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCCGCACAAGATGCa                                                                                                                                  >  1:793696/1‑148 (MQ=255)
                       gCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGaaaa                                                                                                                             <  2:1219185/148‑1 (MQ=255)
                        cATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGaaaaa                                                                                                                            >  2:281084/1‑148 (MQ=255)
                        cATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGaaaaa                                                                                                                            <  1:3440422/148‑1 (MQ=255)
                          tGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaaca                                                                                                                          >  1:2626188/1‑148 (MQ=255)
                          tGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaaca                                                                                                                          >  2:1931135/1‑148 (MQ=255)
                            gCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaacaac                                                                                                                        <  2:2853409/148‑1 (MQ=255)
                              ggACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTg                                                                                                                      <  2:3546279/148‑1 (MQ=255)
                               gACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTgg                                                                                                                     >  2:2024286/1‑148 (MQ=255)
                                aCTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGc                                                                                                                    <  2:2309115/148‑1 (MQ=255)
                                aCTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCCGAAGAAGATTCAGAAAAACAACTGGc                                                                                                                    >  1:138629/1‑148 (MQ=255)
                                  tGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAg                                                                                                                  >  2:235662/1‑148 (MQ=255)
                                    gTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGaa                                                                                                                <  1:118132/148‑1 (MQ=255)
                                      cGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACa                                                                                                              <  2:2611317/148‑1 (MQ=255)
                                         ttCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTc                                                                                                           <  1:1445506/148‑1 (MQ=255)
                                         ttCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTc                                                                                                           <  2:3531018/148‑1 (MQ=255)
                                         ttCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTc                                                                                                           <  1:3218065/148‑1 (MQ=255)
                                         ttCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTc                                                                                                           <  1:3217986/148‑1 (MQ=255)
                                          tCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAAAAACAGGCAGAAACGTCt                                                                                                          >  1:2832224/1‑148 (MQ=255)
                                            tGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGc                                                                                                        >  1:2295910/1‑148 (MQ=255)
                                              gCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCtt                                                                                                      <  2:1441045/148‑1 (MQ=255)
                                                      gatgatGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGa                                                                                              <  2:1538782/148‑1 (MQ=255)
                                                      gatgatGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGa                                                                                              <  1:2507199/148‑1 (MQ=255)
                                                       atgatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                                                                             >  2:1062028/1‑148 (MQ=255)
                                                       atgatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                                                                             <  2:555708/148‑1 (MQ=255)
                                                       atgatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                                                                             >  2:3286410/1‑148 (MQ=255)
                                                         gatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGGGCGGCGCGCAGAAGAAGATGCAGAAAAAAAAATTGCAGAACACTCTGCTTAATAAGGAGtt                                                                                           >  1:1832017/1‑148 (MQ=255)
                                                         gatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                                                                           <  2:2339654/148‑1 (MQ=255)
                                                         gatgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACAGGCAGAACAGTCTGCTTAATAAGGAGtt                                                                                           >  1:2567505/1‑148 (MQ=255)
                                                           tgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtttt                                                                                         <  1:336204/148‑1 (MQ=255)
                                                           tgCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtttt                                                                                         >  1:1871723/1‑148 (MQ=255)
                                                              aaGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAt                                                                                      <  1:2971450/148‑1 (MQ=255)
                                                              aaGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAt                                                                                      <  2:1513279/148‑1 (MQ=255)
                                                                ggCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAATGAGTTTTGATTa                                                                                    >  1:2678240/1‑148 (MQ=255)
                                                                 gCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTAt                                                                                   <  2:712593/148‑1 (MQ=255)
                                                                 gCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTAt                                                                                   <  1:2876434/148‑1 (MQ=255)
                                                                 gCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTAt                                                                                   <  1:2876431/148‑1 (MQ=255)
                                                                      ttctCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCCCAGAAGAAGATGAAGAAAAAAAAAAAGCACAACACTCAGCATAATAACGACTGTTTATTATTAccc                                                                              >  1:1323647/1‑147 (MQ=255)
                                                                      ttctCCATCGGTATCGCCTTGATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCCGCAGAAAAATCACAAAAAAAACGTGCACAACAGTCTGCATAATAAGGAGATTTGATTATGAccc                                                                              >  1:3099750/1‑147 (MQ=255)
                                                                           cATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacgt                                                                         <  1:1626431/148‑1 (MQ=255)
                                                                            aTCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGTCcgtacgga                                                                        >  2:2802885/1‑146 (MQ=255)
                                                                            aTCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacgta                                                                        >  2:2161346/1‑148 (MQ=255)
                                                                            aTCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACAGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATTAAcgtacgta                                                                        >  2:2715464/1‑148 (MQ=255)
                                                                            aTCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAAAAACAGGCAGAACAGTCTGCTTAATAAGGATTTTTGATTATTTCcgtaagta                                                                        >  2:932839/1‑148 (MQ=255)
                                                                               ggTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTc                                                                     <  1:1931135/148‑1 (MQ=255)
                                                                                 tATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTg                                                                   >  2:1124322/1‑148 (MQ=255)
                                                                                 tATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTg                                                                   >  2:1789268/1‑148 (MQ=255)
                                                                                   tCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGc                                                                 >  2:1470699/1‑148 (MQ=255)
                                                                                    cGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCt                                                                >  2:1900089/1‑148 (MQ=255)
                                                                                     gCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtg                                                               <  2:302667/148‑1 (MQ=255)
                                                                                     gCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAAAAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtg                                                               >  1:2386510/1‑148 (MQ=255)
                                                                                      ccTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgt                                                              <  2:1398786/148‑1 (MQ=255)
                                                                                        tttATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATTACCGTAAGTATTATGGCtgtgt                                                            >  2:1557837/1‑148 (MQ=255)
                                                                                        tttATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgt                                                            >  1:2086309/1‑148 (MQ=255)
                                                                                         ttATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATTCAGAAAAAAAAATGGCACAACAGTCTGCTTAATAAGGCGTTTTGATTATGACCGTACGTATTATTGCtgtgtg                                                           >  1:2526658/1‑148 (MQ=255)
                                                                                          tATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGGGGCGCGCCGAAGCAGATTCAGAAAAAAAAAAGGCAGTACACTCTGGGTAAAAAGGAGTTTTTATTATGACCGGACGGTTTCTGGCtgtgtgt                                                          >  1:363821/1‑148 (MQ=255)
                                                                                          tATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtgt                                                          <  1:2795523/148‑1 (MQ=255)
                                                                                            tGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTgg                                                        >  2:992572/1‑148 (MQ=255)
                                                                                               ccGTCATCATTGTAATTGCACTGGCTTATATGGTCGTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCaa                                                     <  2:2916820/148‑1 (MQ=255)
                                                                                                   catcatTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGa                                                 >  1:2046010/1‑148 (MQ=255)
                                                                                                     tcatTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCCGAAGAAGATGCAGAAAAAAAACAGGCCGAACACTGTGGGTAATAATGAGTTTTTTTTATTAACGTACGTATTATGGCTGTGTGTGGGAAAGGACa                                               >  1:477925/1‑148 (MQ=255)
                                                                                                      catTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGGGCTGCGCGCAGAAGAAGATTCAGAAAAACAACTGGCAGAACAGTCTTCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTTTGGCAACGGACaa                                              >  2:3289009/1‑148 (MQ=255)
                                                                                                      catTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATTCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGTTTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACaa                                              >  1:584335/1‑148 (MQ=255)
                                                                                                      catTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACaa                                              >  2:2371026/1‑148 (MQ=255)
                                                                                                        ttGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTTATTATGAACGTACGTATTCTGGCTGTGTGTGGCAACGGACAAgg                                            >  2:427452/1‑148 (MQ=255)
                                                                                                        ttGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAgg                                            >  2:3350671/1‑148 (MQ=255)
                                                                                                         tGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGc                                           >  2:3069764/1‑148 (MQ=255)
                                                                                                            aaTTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAATAAGATGAAGAAACACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGt                                        <  1:1240389/148‑1 (MQ=255)
                                                                                                            aaTTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGt                                        <  1:1857150/148‑1 (MQ=255)
                                                                                                             aTTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGtt                                       >  2:3169589/1‑148 (MQ=255)
                                                                                                               tGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGttgc                                     >  2:287099/1‑146 (MQ=255)
                                                                                                                   cTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGa                                 <  1:2154973/148‑1 (MQ=255)
                                                                                                                    tGGCTTATATGTTCTTCGCTGGCTGCGCGCTGCGCGCAGAAGAAGATGCAGAAACACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGAt                                <  2:252863/148‑1 (MQ=255)
                                                                                                                    tGGCTTATATGTTCTTCGCTGGCTGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGAt                                <  2:252799/148‑1 (MQ=255)
                                                                                                                     ggCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATc                               <  1:2746593/148‑1 (MQ=255)
                                                                                                                     ggCTTATATGTTCTTCGCTGGCCGCGCGCGGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCCTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCCAGATc                               >  2:3033512/1‑148 (MQ=255)
                                                                                                                          atttGTTCTTCGCTGGCCGCGCGCTGCGCGGTTAAGAAGATGCAGAAAAACAACTGTCTGAAGAGTCTGCTTTATAAGGATTTTTGATTTTGACCGTAGGTATTGTGGATGTGTGTGGCAACGGTCAAGGCAGTTCCATGATCATGaa                          <  2:2571715/145‑1 (MQ=255)
                                                                                                                              gTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATg                      >  2:3448005/1‑148 (MQ=255)
                                                                                                                                tcttcGCTGGCCGCGCGCTGCGCGCCGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTTATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCCTGATCATGAAGATGaa                    >  2:2481123/1‑148 (MQ=255)
                                                                                                                                tcttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTTATTATTACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGaa                    >  1:2089954/1‑148 (MQ=255)
                                                                                                                                tcttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGaa                    <  1:1183918/148‑1 (MQ=255)
                                                                                                                                tcttcGCTGGCCGCGCGCGGCGCGCAGAAGAAGATTCAGAAAAACAACGGGCAGAACAGTCGGCTTAATAAGGAGTTTTGATTATGACCGTAAGGATTCTGGCTGTGTGTGGCAACGGACAAGGCAGGTCCATGATCATGAAGATGaa                    >  1:1156881/1‑148 (MQ=255)
                                                                                                                                 cttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGaaa                   <  2:1434249/148‑1 (MQ=255)
                                                                                                                                  ttcGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAg                  >  2:1414899/1‑148 (MQ=255)
                                                                                                                                       tGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGAc             <  2:27254/148‑1 (MQ=255)
                                                                                                                                         gCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCACTTCCATGATCATGAAGATGAAAGTGGACCa           >  2:432954/1‑148 (MQ=255)
                                                                                                                                             cgcgcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGttt       <  2:2295910/148‑1 (MQ=255)
                                                                                                                                             cgcgcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGttt       <  2:2291070/148‑1 (MQ=255)
                                                                                                                                               cgcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGttttt     >  1:1669749/1‑148 (MQ=255)
                                                                                                                                                gcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTa    <  1:2326389/148‑1 (MQ=255)
                                                                                                                                                gcTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTa    <  1:3303812/148‑1 (MQ=255)
                                                                                                                                                 cTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTaa   >  1:3300822/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAGATGAAAGTGGACCAGTTTTTAAC  >  CP009273/4410999‑4411292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑